晚期前列腺癌并发症的处理(附42例报告)

目的　提高晚期前列腺癌并发症的治疗水平。方法　对 42例晚期前列腺癌患者分别出现的骨痛、贫血、尿路梗阻、顽固性血尿及下肢水肿等并发症分别给予多种止痛剂、博宁、氯化锶、外放射、间断输血、经尿道前列腺电切术等治疗。结果　 42例患者中 1 8例死亡 ,2 4例存活 ;结论　对晚期前列腺癌患者常见并发症的处理应采取综合措

尿细胞角蛋白19在膀胱移行细胞癌术后监测中的应用

目的　评价尿细胞角蛋白19片段(CK19,又称CYFRA2 1 1)在膀胱移行细胞癌(BTCC)术后监测中的作用。方法　对67例保留膀胱的BTCC术后患者定期行膀胱镜检查发现2 1例复发,采用电化学发光免疫分析(ECLIA)技术测定复发组(2 1例)、未复发组(4 6例)和正常对照组(2 4例)的尿CKl9含量。结果　复发组尿CKl9的平均值为(19.60±18.5 7) μg/L ,明显高于未复发组(2 .3 3±1.64 ) μg/L和正常对照组(1.82±0 .83 ) μg/L。复发组与未复发组及正常对照组比较,差异有统计学意义(P <0 .0 1)。尿CKl9对BTCC复发的敏感性为90 .5 % ,特异性为76.0 %。结论　尿CKl9是监测BTCC术后复发较为敏感、特异而且快速无创的指

Comparison of efficacy between mPCNL with holmium laser and EMS standard tract PCNL in the treatment of complex renal calculi under ultrasound guidance

目的比较b超引导下微通道经皮肾镜钬激光碎石术(MPCnl)与标准通道经皮肾镜气压弹道联合超声碎石术(标准通道PCnl)治疗复杂性肾结石的临床疗效。方法对78例复杂性肾结石患者分别行MPCnl(35例)和标准通道PCnl(43例)治疗,术后对2组患者的手术时间、一期结石清除率和手术并发症等指标进行比较分析。结果 2组患者分别成功建立16f和24f经皮肾通道并一期行碎石术。MPCnl组手术时间明显长于标准通道PCnl组;一期结石清除率MPCnl组低于标准通道PCnl组,差异均具有统计学意义(P均38.5℃1例。2组并发症差异无统计学意义(P>0.05)。结论 b超引导下MPCnl与标准通道PCnl治疗复杂性肾结石安全有效、并发症发生率低,标准通道PCnl联合应用EMS第4代气压弹道联合超声碎石清石系统碎石清石效率高,较MPCnl钬激光碎石明显有优势,值得推广。Objective To compare the outcomes of the surgical treatment of complex renal calculi with minimally invasive percutaneous nephrolithotomy(mPCNL) and EMS percutaneous nephrolithotomy(standard tract PCNL) under ultrasound guidance.Methods A total of 78 patients with complex renal calculi were allocated to either mPCNL treatment group(mPCNL group,n=35) or EMS(swiss,the fourth generation) standard tract PCNL treatment group(standard tract PCNL group,n=43).The operative time,primary stone-free rate and operative complications in the two groups were compared.Results 16F and 24F percutaneous renal accesses were successfully established in mPCNL group and standard tract PCNL group respectively,and then the first lithotrity-lithiasis cleaning procedure was performed.The operation time in mPCNL group was longer than that in the standard tract PCNL group 〔(102±31) min vs(67±22) min,P38.5 ℃ occurred in one case of mPCNL group.There was no significant difference in operative complications in two groups(P>0.05).Conclusions Both the treatments of complex renal calculi with mPCNL and EMS(the fourth generation) standard tract PCNL under ultrasound guidance are similarly safe,but the standard tract PCNL has the superiority of higher primary stone-free rate and shorter operation time over the holmium Laser mPCNL.Therefore the standard tract PCNL merits propagation for treating complex renal calculi

前列腺移行带腺体定量分析

目的通过定量分析良性前列腺增生(BPH)患者前列腺和成人正常前列腺移行带腺体各成分含量及相关组织学形态的变化,探讨良性前列腺增生症治疗方案的选择。方法用计算机图像分析系统对经HE染色的40例良性前列腺增生症患者手术切除的前列腺和10例成人正常前列腺移行带组织标本进行形态学定量分析,分别测定腺体、腺腔、上皮的平均面积及上皮细胞高度。结果(1)正常前列腺和BPH前列腺移行带上皮细胞高度分别为(24.87±4.53)μm和(19.06±5.49)μm(P0.05)。结论BPH前列腺组织主要以基质成分增生为主;BPH的治疗应以α受体阻滞剂和手术治疗为主

深度闭合性肾裂伤的非手术治疗

总结25例深度闭合性肾裂伤的救治经验。认为对深度闭合性肾裂伤,可根据具体情况,在严密监护下行非手术治疗,效果满意

模拟氮沉降对干旱半干旱温带草原土壤细菌群落结构的影响

随着社会经济发展,活性氮源随人为活动逐年递增,导致氮沉降相应增加,由此带来的生态环境问题也日益严重.本研究基于细菌16S rRNA基因高通量测序技术,分析长期人工模拟氮沉降氮输入量为0、1、4、8、32 g·(m~2·a)~(-1)对干旱半干旱区草原土壤微生物群落结构的影响.结果表明,模拟氮沉降7 a增加了土壤中可利用氮含量,引起草原土壤酸化.高通量测序结果表明,细菌OTU(operational taxonomy unit)数量随氮输入量的增加呈现增加的趋势,高氮处理[8 g·(m~2·a)~(-1)和32g·(m~2·a)~(-1)]显著高于低氮和对照处理;细菌α多样性指数呈现先增加后降低的趋势.不同氮输入水平下土壤细菌群落结构差异显著,且与土壤p H和氮的有效性显著相关.不同细菌相对丰度在纲的水平对氮输入的响应趋势不同.氮的长期大量输入可直接或间接通过改变土壤性状而影响草地土壤细菌群落结构和多样性

EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells

National Natural Science Foundation of China [21035006]EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit-8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid-mediated pcDNA3.1(+)-EphA2, markedly enhanced the cell growth and invasion in vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of Eph.A2 was significantly associated with Gleason grade, total prostate-specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EptLA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer

Europium-labeled activity-based probe through click chemistry: Absolute serine protease quantification using 153Eu isotope dilution ICP/MS

Click and analyze: The titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with 153Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one 153Eu(NO3)3 isotopic standard. Copyright 漏 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim