Design and implementation of the Yunnan College of Business Management Enrollment Management Statistical Analysis System

随着高等职业教育的大众化和办学模式多样化，以及民办高职院校扩招规模的不断扩大引起了激烈的生源竞争，尤其是优质教育资源的竞争，为了保证生源，民办高职院校间的竞争越来越激烈，在这种状况之下，民办高职院校要适应形势的发展，争取生源，做好招生工作就显得十分必要。 本课题的主要任务是根据云南经济管理学院招生管理业务的发展需求设计并实现一个基于MicrosoftVisualStudio.NET开发平台的三层架构的招生管理情况统计分析系统。论文首先分析了云南经济管理学院招生工作的现状，简要说明了论文的研究内容。其次，对云南经济管理学院招生管理统计分析系统进行了详细的功能需求分析，包括历年招生录取综合情况统...With the higher vocational education popularization and running mode of diversification, and private higher vocational college enrollment scale unceasing expansion causes the fierce competition for students, especially high quality education resources competition, to ensure the students, competition between colleges and universities is becoming more and more fierce. Under this situation, private h...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201323134

Design and Implementation of Administrative Institution Assets Tracking System

伴随着事业单位规模的不断扩大，原始的、手工地进行固定资产管理工作已经不能适应行政事业单位固定资产管理的现状，更无法满足行政事业单位对管理工作的快速、准确的要求。而计算机信息管理技术的迅速发展恰恰解决了这个问题，它使计算机技术与现代的管理技术相互配合，来更加准确、高速的完成企业日常的固定资产管理工作，使企业能够以最少的人员来完成更多的工作，工作的可靠性和效率有了大幅度的提高。本系统采用了Silverlight技术，极大地提高了软件用户体验，将资产数据展现出丰富的图表样式。采用条码管理的技术，盘点人员可以直接通过专门的手持终端对固定资产进行盘点。同时很好的兼容了国家对行政事业单位的十一个大类，方便...With the constant expansion of enterprises, the original, manual management work on fixed assets can not adapt to the present development of enterprises, and can't satisfy speed and accuracy requirements of enterprise management. But the rapid developing computer information management technology can solve the problem, it makes computer technology and modern management technology cooperate , to co...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201023015

Determination of Organophosphorus Pesticides in Seawater by GC-FPD Using Two Columns

本文提出了一种GC-FPD测定海水中28种有机磷农药残留的新方法。采用双柱法,即选用DB-1701柱进行定性、定量分析,用HP-5柱验证。既发挥了FPD检测器灵敏、定量准确的优点,又利用了双柱法的定性功能。方法对各目标物均有良好的线性关系,回收率和RSD分别在70.2%~103.2%、8.7%~17.5%之间,适合于海洋水体中痕量有机磷农药的分析。A GC method has been developed to determine 28 kinds of OPPs in seawater simultaneously by GC-FPD.The columns were DB-1701 and HP5.The column DB-1701 used for analysis and the column HP-5 used for validate.The linear dynamic ranges were well.the recovery was from70.2 %～103.2 %,the RSD was from 8.7 %～17.5 %.The method was used for the determination of trace OPPs in sea water.国家863项目(项目编号:2003AA635180

Optimization of separation methods and culture system of chicken embryonic stem cells in vitro

背景:胚胎干细胞是从动物早期胚胎的内细胞团或原始生殖细胞分离出来的具有发育全能性的一种未分化的无限增殖细胞系。而鸡胚胎干细胞则是从X期鸡胚的胚盘分离而来。目的:优化鸡胚胎干细胞分离方法和离体培养体系。方法:采用滤纸纸环-发环的方法从X期鸡胚分离胚盘细胞,并采用STO细胞作为饲养层和大鼠肝细胞(brl)条件培养基(CM)+细胞因子作为离体培养体系对分离的胚盘细胞进行培养。结果与结论:滤纸纸环-发环法获得的完整胚盘率为75%~85%,克隆形成率约为50%。brl-CM+饲养层培养体系,鸡胚胎干细胞可传至7代,而brl-CM+饲养层+细胞因子培养体系,鸡胚胎干细胞可传至25代。分离到的鸡胚胎干细胞,经碱性磷酸酶染色、SSEA-1染色鉴定,表明鸡胚胎干细胞处于未分化状态。提示,实验不仅优化了鸡胚胎的分离方法,获得完整且杂质少的胚盘,而且进一步优化了鸡胚胎干细胞体外培养体系。BACKGROUND:Embryonic stem cells are undifferentiated permanent cell line derived from inner cell mass cells and primordial germ cells of animal's early embryos.Chicken embryonic stem cells are derived from the blastodermal of a X-stage embryo.OBJECTIVE:To optim the separation method and in vitro cultural system of chicken embryonic stem cells.METHODS:The X-stage chicken embryos were isolated by using a small square of ?lter paper with a hole punched in the center,and the blastodermal cells were isolated by using the hair loop.STO cells were used to make feeder layer;at the same time,BRL-CM and cytokine were also used for chicken embryonic stem cells in vitro cultural system.RESULTS AND CONCLUSION:The filter paper loop and the hair loop could obtain complete the blastoderm,and the successful percentage was 75%-85%.The colony formation rate was about 50%.After culture in the BRL-CM + feeder layer + cytokine culture system,the passage of CES cells is the seventh generation;BRL-CM + feeder layer + cytokines,cultured chicken embryonic stem cells could passage to the 25th generation.Isolated chicken embryonic stem cells were in an undifferentiated state detected by alkaline phosphatase staining and SSEA-1 staining.The findings indicate that this experiment not only optimized the isolation method of chicken embryonic stem cells to obtain complete and pure embryos,but also further improved the in vitro culture system of chicken embryonic stem cells.国家973项目(2009CB941600)资助;国家自然科学基金项目(31072101)资助---

50型轮胎式装载机异常噪声故障诊断

某单位生产的轮胎式装载机在出厂试车时发现部分装载机有异常噪声。通过对有异响和无异响装载机在不同转速下进行的噪声对比测量、分析及计算,查出了异常噪声原因,提出诊断意见和建议措施,使问题得以解决

SiO2 supported IB metals alloyed Pd single-atom catalysts (SACs) for selective hydrogenation of acetylene to ethylene in excess ethylene

SiO2 supported IB metals alloyed Pd single-atom catalysts (SACs) for selective hydrogenation of acetylene to ethylene in excess ethylen

5个构树地方品种耐寒性状研究

【目的】为了筛选构树可以用于全基因组关联分析的耐寒指标。【方法】在构树越冬前芽组休眠的过程中截取枝条顶端部位,测定含水量、木质素含量以及木质部占横截面的比例,分析不同种源构树耐寒性。【结果】在越冬前休眠的过程中,北方来源的构树枝条含水量低于南方来源的构树,木质部占横截面的比例以及木质素含量高于南方来源的构树,而枝条含水量、木质部占横截面的比例以及木质素含量直接影响植株的耐寒性。【结论】南北方构树在越冬的过程中对低温的抵抗能力有显著的差异,枝条含水量、木质部占横截面的比例以及木质素含量直接影响构树的安全越冬

水孔蛋白协助玉米副卫细胞中Ｈ２Ｏ２ 的跨膜转运

【目的】探索玉米气孔副卫细胞中Ｈ２Ｏ２ 的来源，以阐明禾本科植物气孔运动过程中保卫细胞和副卫 细胞协同调节的机理。【方法】采用细胞化学方法对Ｈ２Ｏ２ 进行亚细胞荧光定位，以Ｔｕｂｕｌｉｎ 和ＧＡＰＤＨ 为内参基 因，利用ｑＰＣＲ技术，对玉米水孔蛋白基因ＺｍＰＩＰ２；４、ＺｍＰＩＰ２；５和ＺｍＰＩＰ２；６表达量进行分析，从而确定水孔蛋 白协助玉米副卫细胞中Ｈ２Ｏ２ 的跨膜转运。【结果】光暗处理组，加水孔蛋白抑制剂ＡｇＮＯ３ 与不加ＡｇＮＯ３ 副卫细胞 中的Ｈ２Ｏ２ 积累相反；外源Ｈ２Ｏ２ 引起副卫细胞中Ｈ２Ｏ２ 积累，先加入水孔蛋白抑制剂ＡｇＮＯ３ 再加外源Ｈ２Ｏ２ 处理 后副卫细胞中Ｈ２Ｏ２ 不积累；短细胞发生后，副卫细胞中的Ｈ２Ｏ２ 开始积累，同时ＺｍＰＩＰ２；５基因的相对表达量上调； 随着水分胁迫程度增加，ＺｍＰＩＰ２；４、ＺｍＰＩＰ２；５、ＺｍＰＩＰ２；６基因的相对表达量随短细胞发生上调。【结论】水孔蛋白具 有转运Ｈ２Ｏ２ 的功能，玉米叶片下表皮副卫细胞中的Ｈ２Ｏ２ 是外源的，其积累和清除可能与水孔蛋白ＺｍＰＩＰ２；５的转运 有关。</p

Ga-Fe_3O_4的室温合成和谱学表征

采用氧化-沉淀法在室温下合成了不同Ga含量的Ga取代磁铁矿,并对上述产物进行了IR,XRD,Mossbauer等谱学解析和磁滞回线的测量。结果表明：Ga取代磁铁矿的生成可能经过Ga取代的绿锈中间体,但这种中间体在热N2干燥的条件下就能转化成产物磁铁矿;随Ga进入尖晶石结构中,产物的XRD分析表明其晶面间距减小,IR光谱分析表明标志MT—O—MO的振动吸收峰向高波数移动。少量的Ga进入尖晶石结构优先占据A位,且初始溶液中含有少量Ga^3＋能一定程度抑制非磁性的Fe2O3的生成;随Ga含量的增加,其进入B位的趋势增加