Study on the New Industrialization Development Issues of State-owned Industrial Groups in Chongqing

工业化是现代化的基础和前提，高度发达的工业社会是现代化的重要标志。党的十六大报告指出：在本世纪头二十年我国经济建设的主要任务之一，是基本实现工业化，并明确提出“要坚持以信息化带动工业化，以工业化促进信息化，走出一条科技含量高、经济效益好、资源消耗低、环境污染少、人力资源得到充分发挥的新型工业化路子”。本论文根据党的十八大和重庆市第四次党代会关于新型工业化的基本要求，通过对国内外新型工业化的发展现状的分析研究，总结其经验教训；再结合重庆实际，深入剖析“十一五”期间市属工业集团在新型工业化发展过程中取得的成绩，存在的不足，面临的机遇和挑战，提出“十二五”期间重庆国有工业集团新型工业化的宏观战略和具...Industrialization is the basis and premise of modernization, a highly developed industrial society is an important symbol of modernization. The Sixteenth CPC National Congress had indicated that one of the main tasks of China’s economy construction in the first two decades of this century was basically accomplishing industrialization. It also indicated the definition of New Industrialization at th...学位：工商管理硕士院系专业：管理学院高级经理教育中心（EMBA项目）_工商管理硕士(高级管理人员工商管理硕士)学号：X201315632

Analysis about ultrasonic misdiagnosis of lower extremity venous thrombosis

目的通过对下肢静脉血栓超声患者的超声资料进行回顾性分析,总结分析误诊漏诊的原因方法对70例临床怀疑为下肢静脉血栓的病人进行常规静脉彩超检查,对确诊为下肢静脉血栓的患者再行CT静脉造影检查,而后对两者诊断结果进行对照分析。结果 70例临床疑似为下肢静脉血栓病人中,确诊率为57.1%（40/70）,超声误诊率32%（8/25）。结论超声检查无创伤、重复性高,可准确诊断下肢静脉血栓形成。Objective The data of patients with lower extremity venous thrombosis were analyzed retrospectively, and the causes of misdiagnosis and missed diagnosis were summarized. Methods 70 patients with clinical suspicion of lower extremity venous thrombosis were treated by routine venous color Doppler ultrasonography, and the venous thrombosis was diagnosed by CT venous angiography.Results Among 700 patients with clinical suspicion of lower extremity venous thrombosis, the accuracy rate was 57.1%, and the misdiagnosis rate was 32%. Conclusion Because ultrasound examination has high repeatability and no trauma, it can be used to diagnose exactly the lower extremity venous thrombosis.昌吉回族自治州科技局项目（TG-201405

Over-expression of Pygo2 Promotes C6 Cells Proliferation of Glioma

目的通过构建过表达PygO2的重组体上调PygO2表达,探讨其在大鼠胶质瘤C6细胞增殖中的作用及机制。方法重组体经ECOr I和HIndⅢ双酶切鉴定和dnA测序后,用脂质体2000将其转染大鼠胶质瘤C6细胞,采用WESTErn blOT检测外源PygO2蛋白表达,应用克隆形成实验和MTT法检测细胞增殖,流式细胞术检测细胞周期,采用WESTErn blOT检测过表达PygO2对C6细胞中CyClInd1、β-CATEnIn水平的影响,并采用细胞免疫荧光法检测其对C6细胞中CyClInd1、β-CATEnIn亚细胞定位的影响。结果双酶切和测序鉴定结果证实插入序列正确,重组体能有效上调PygO2表达。将重组体转染C6细胞上调PygO2表达后,细胞的生长增殖被显著促进,克隆形成显著增多,细胞周期进程从g1期至S期转变显著增强;且CyClInd1水平随之增高,亚定位无改变,β-CATEnIn水平和亚细胞定位无明显改变。结论成功构建了过表达PygO2的重组体,过表达PygO2通过增高CyClInd1水平,促进细胞从g1期进入S期,从而促进大鼠胶质瘤C6细胞增殖。Objective To up-regulate expression of Pygopus2(Pygo2) by construction of the recombinant vectors of over-expression of Pygo2 protein,and to explore the role and mechanism of over-expression of Pygo2 in C6 cells proliferation of glioma.Methods The recombinant plasmids were digested with EcoRⅠ and Hind Ⅲ to execute the restriction endonuclease identification,then the sequence analysis was assayed by DNA sequencing.The recombinant plasmids were transfected into cultured glioblastoma C6 cells using lipofectamineTM 2000.The exogenous Pygo2 protein level of C6 cells was detected by Western blot analysis.Colony forming assay and MTT assay were used to detect the cell proliferation,and cell cycle analysis was performed by flow cytometry analysis.The effect of Pygo2 over-expression on the level of cyclinD1 and β-catenin of C6 cells was detected by Western blot analysis,and the expression and subcellular location of cyclinD1 and β-catenin of C6 cells were further quantified by immunofluorescent staining.Results The recombinant plasmids were completely coincided with the designs by the restriction map and the sequence analysis,which up-regulated Pygo2 expression of C6 cells efficiently.After Pygo2 expression were up-regulated by transfected C6 cells with the recombinant plasmids,cells proliferation was promoted and colony forming was increased significantly,cell cycle progression from G1 to S transition was enhanced notably.Furthermore,the expression level of cyclinD1 was significantly increased without change of subcellular location,and the expression level and subcellular location of β-catenin were not changed obviously.Conclusion The recombinant vectors of Pygo2 over-expression were constructed successfully.Over-expression of Pygo2 promotes the growth of glioma cells by an increased expression of cyclinD1 to improve G1/S transition.重庆市自然科学基金资助项目(cstc2011jjA10110);重庆市教委科技基金资助项目(KJ100504);福建省自然科学基金资助项目(2009D002

磷酰化氨基酸酯的合成、波谱表征及生物活性

磷酰化氨基酸及其酯属于磷酰胺类,它们作为天然氨基酸的含磷类似物,具有良好的生物活性,它的合成与性质的研究引起人们的浓厚兴趣.本工作用亚硫酰氯先合成磷酰化酰氯,再由其与氨基酸甲酯反应合成了一系列磷酰化氨基酸酯,产物经红外光谱,核磁共振氢谱、碳谱、磷谱,质谱及元素分析鉴定,并初步研究这些化合物的抗菌与抗肿瘤的生物活性

五配位氧磷烷分子间配体交换反应-RNA水解和融合过程的化学模型（英文）

具有五元环和三配体结构的五配位氧磷烷(ab2)在碱催化条件下自发进行分子间的配体交换反应,产生不同配体组合的全部三种五配位氧磷烷(a3,b3和a2b)。如果把其中a3与b3作为父代分子,其配体交换产生的五配位氧磷烷a2b和ab2可以视作子代分子,从而自发实现了分子结构的多样化。因此,五配位氧磷烷分子间配体交换反应可以作为研究生命过程中具有五配位磷中间体结构化学性质的模型,对理解基因转录和生命信息储存等过程中涉及的RNA分子剪接、水解和融合等重要生命过程的分子机制提供了重要依据

五配位氧磷烷分子间配体交换反应-RNA水解和融合过程的化学模型（英文）

具有五元环和三配体结构的五配位氧磷烷（ab2）在碱催化条件下自发进行分子间的配体交换反应,产生不同配体组合的全部三种五配位氧磷烷（a3,b3和a2b）.如果把其中a3与b3作为父代分子,其配体交换产生的五配位氧磷烷a2b和ab2可以视作子代分子,从而自发实现了分子结构的多样化.因此,五配位氧磷烷分子间配体交换反应可以作为研究生命过程中具有五配位磷中间体结构化学性质的模型,对理解基因转录和生命信息储存等过程中涉及的RNA分子剪接、水解和融合等重要生命过程的分子机制提供了重要依据.Project supported by the National Natural Science Foundation of China(Nos.21778042,41876072,21772163,41576081)the Xiamen Southern Oceanographic Center(No.17GYY002NF02)the Fundamental Research Funds for the Central Universities(No.20720170069)~

集美大学2002级福建籍新生肝炎感染现状

目的　了解集美大学 2 0 0 2级福建籍新生甲、乙、戊 3型肝炎感染情况。方法　采用血清流行病学调查方法。结果　学生中抗HAV -IgG ,HBsAg和抗HEV -IgG阳性率分别为 77.0 8% ,1 5 .2 3 % ,1 7.1 5 % ,地区差异明显 ;HAV ,HEV无性别差异 ,而HBV以男性为高 ;农村HAV和HBV感染率均高于城镇 ,而HEV感染率无城乡差别。结论　应该加强对本地区大学生的甲、乙肝预防 ,急需研究出一种有效的戊肝疫

Zooplanktonic diversity in the western Pacific

西太平洋区是全球海洋生物种源中心,许多类群的最高物种多样性都出现于该区域,因此,在该区开展种类多样性的研究不仅重要和必要,而且具有在跨国尺度上进行综合管理和相互合作的迫切性。本文在西太平洋的浮游动物样品鉴定分类、编目、文献资料整理和分析的基础上,记录和编入西太平洋10个浮游动物类群2,658种(含亚种),隶属于206科675属,其中水螅水母类99科251属697种,栉水母类12科22属59种,浮游软体动物14科35属86种,介形类8科89属416种,桡足类51科156属908种,糠虾类4科58属202种,磷虾类2科8属56种,十足类8科22属105种,毛颚类5科8属48种,被囊类5科26属81种。The western Pacific region has been operating as a centre for the origin of marine biodiversity: the richest diversity of many marine taxa was found in these waters.Therefore,biodiversity research and con-servation efforts in this area are necessary in order to promote the integrative and international management of this resource.The present work is a compilation of numbers of all the families,genera and species of ma-jor taxa of zooplankton known in the western Pacific Ocean(106°–150°E,0°–44°N).In all,2,658 zooplank-tonic species(including subspecies) belonging to 206 families and 675 genera have been recorded from taxonomic identifications and literature,99 families,251 genera and 697 species belong to the Medusozoa,12 families,22 genera and 59 species to the Ctenophora,14 families,35 genera and 86 species to the pelagic Molluscs(Pteropoda and Heteropoda),8 families,89 genera and 416 species to the Ostracoda,51 families,156 genera and 908 species to the Copepoda,4 families,58 genera and 202 species to the Mysidcea,2 fami-lies,8 genera and 56 species to the Euphausiacea,8 families,22 genera and 105 species to the Decapoda,5 families,8 genera and 48 species to the Chaetognatha,5 families,26 genera and 81 species to the Tunicata.908专项“中国海洋生物种类名录和图谱”(908-ZC-II-02)和908专项海洋生物样品库;科技部基础工作专项“我国和邻近西北太平洋海洋生物物种编目和分布图集编制”(2006FY220700

Prevalence of hepatitis B virus infection and phylogenetic analysis of HBV S gene among high school students

目的了解江苏省海门市高中生乙型肝炎感染情况及其HbV S基因的变异情况。方法采用整群抽样方法抽取江苏省海门市职业中心校的407名高三学生进行乙肝血清学调查;采用酶联免疫吸附法(ElISA)和巢氏聚合酶链式反应(PCr)扩增方法检测血清样本的乙型肝炎表面抗原(HbSAg)、乙型肝炎病毒表面抗体(抗-HbS)和乙型肝炎病毒(HbV)S基因并进行进化分析。结果 407名高中生中乙肝表面抗原(HbSAg)阳性率为6.39%;在家中、乡镇卫生院和县级及以上医院出生学生的HbSAg阳性率分别为11.76%、8.02%和2.27%,差异有统计学意义(P<0.05);抗-HbS滴度≥10u/l者占72.5%,且呈u形分布;不同乡镇、不同性别学生抗-HbS滴度差异有统计学意义(P<0.01);407名高中生HbV S基因均为C基因型,主要变异在蛋白疏水区,抗原决定簇A区未发生免疫原性改变。结论出生时接受的医疗水平对HbV感染有影响;海门市高中生HbV S基因受到正选择作用而出现变异,但未发生免疫原性改变。Objective To understand the hepatitis B infection and HBV S gene variation among high school students in Haimen city,Jiangsu province.Methods A serological survey of hepatitis B was conducted in a high school with cluster sampling method.HBsAg and anti-HBs were detected with ELISA,and phylogenetic analysis for HBV S gene with nested PCR.Results HBsAg positive rate was 6.39% and the rate for the students born at home,township health centers,and hospital above the county level was 11.76%,8.02%,and 2.27%,respectively,with significant difference(P<0.05).The percentage of the students with anti-HBs titer ≥ 10U/l was 72.5%,and the titer distribution was U-shaped.The difference in anti-HBs titer between boys and girls,as well as different towns,was statistically significant(P<0.01).All genotype of HBV S gene was C-type,and the main variation was in protein hydrophobic area.The change of immunogenicity in antigenic determinant "a" zone was not observed.Conclusion The level of health care at birth can affect the HBV infection among high school students in Haimen.S gene variation occurs through positive selection,but not antigenicity.国家科技支撑计划(2006BAI02A03);传染病重大专项(2008ZX10002-012);上海市重点学科项目(B118

Synthesis, spectral characterization and biological activities of phosphoryl amino acid esters

Phosphoryl amino acids and phosphoryl amino acid esters, as phosphorus analogs of amino acid, have attracted much attention because they show wide biological activities. In this paper, the tide compounds, 2-isopropoxy-(2-isopropyl-5-methyl-cyclohexyloxy)-phosphoryl amino acid esters, were synthesized in the presence of sulfuryl chloride with reasonable yields. Their structures were confirmed by IR, H-1 NMR, C-13 NMR, P-31 NMR and MS spectra, and the antibacterial and antitumor activities of these compounds were given in this paper