Distribution of HPV genotypes of cervical lesions patients in Zhangzhou district

目的：了解漳州地区人乳头瘤病毒（human papilomavirus,HPV）基因型与宫颈病变的关系.方法：应用基因芯片技术对200例宫颈病变患者石蜡组织标本进行24种HPV基因型检测,以组织病理学诊断作为宫颈病变确诊的金标准.结果：（1）200例标本中,HPV阳性率为51.50 %;检出20种基因型,常见基因型依次是HPV16（27.97 %）、HPV18（8.39 %）、HPV58（8.39 %）、HPV59（7.69 %）、HPV54（7.69 %）;其中低危型HPV占16.08 %,以HPV54、HPV6和HPV70多见;高危型HPV为83.92 %,以HPV16、HPV18、HPV58和HPV59多见;HPV单一感染率与多重感染率分别为67.96 %和32.04 %,以合并HPV16的多重感染为最常见,占42.42 %.（2）HPV感染率随着年龄增加而增加,以年龄≥60岁年龄段阳性率（66.67 %）最高,但各年龄段HPV感染率差异无统计学意义（P＆gt;0.05）.（3）不同类型HPV基因型可引起不同程度宫颈病变,慢性宫颈炎、低级别宫颈上皮内瘤变（L-CIN）、高级别宫颈上皮内瘤变（H-CIN）和宫颈鳞癌,常见基因型分别依次是HPV54、HPV16/HPV51/HPV58;HPV59、HPV53/HPV58;HPV16、HPV58/HPV59;HPV16、HPV18、HPV51/HPV54,而HPV16、HPV18与宫颈病变相关（P＆lt;0.05）.（4）HPV感染率随着宫颈病变进展而增加（P＆lt;0.05）;不同宫颈病变中多重感染率低于单一感染率（P＆lt;0.05）;多重感染、低危型多重感染、高危型感染、高危型多重感染、高低混合型多重感染的各组感染率在不同级别宫颈病变差异有统计学意义（P＆lt;0.05）.结论：随着宫颈病变进展HPV感染率呈升高的趋势,持续高危型感染和多重感染是加重宫颈病变的重要因素,HPV16、HPV18基因型感染不但多见而且使宫颈癌的患病风险增加.Objective：To investigate the relationship between human papilomavirus （HP） genotypes and cervical lesions in Zhangzhou district. Method： The gene chip technology was adopted to test 24 types of HPV genotypes in paraffin tissue from ：200 cases of patients with cervical lesions, and histopathological diagnosis was used as gold standard for cervical lesions confirmation. Results： （1）In the 200 specimens, the positive rate of HPV was 51.50% （103/200）, 20 genotypes were detected, and the common types were HPV16 （27.97 % ）, HPV18 （8.39 % ）, HPV58 （8.39 % ）, HPV59 （7.69 % ） and HPV54 （7.69 % ）. In the HPV posi- tive cases, the rates of low - risk type of HPV were 16.08%, and the common types were HPV54, HPV 6 and HPV70; the rates of high - risk type of HPV were 83. 92 %, and the common types were HPV16, HPV18, HPV58 and HPV59 ; the rates of simple infection and multiple infection were 67.96 % and 32.04 % respective ly, and HPV 16 merged multiple infection was the most common, accounting for 42.42 %. （2） The infection rate of HPV increased with the increase of age, the posi- tive rate in above 60 years group was the highest （66.67 % ）, but there were no significant differences among different age groups （ P 〉 0.05 ）. （~ HPV of different genotype could induce different cervical lesions, such as chronic cervicitis, low - CIN, high - CIN, squamous cell carcinoma, and the common types were HPV54, HPVI6 / HPV51 / HPV58, HPV59, HPV53 / HPV58, HPV16, I-IPV58 / HPV59, HPV16, HPV18, HPV51 / HPV54. HPVI6 and HPVI8 were closely correla- ted with the cervical lesions （ P 〈 0.05 ）. The infection rate of HPV increased with the progression of cervical lesions （ P 〈 0.05 ） ; The rates of multiple HPV in- fection were significantly lower than that of single HPV infection in different cervical lesions （ P 〈0.05 ） ; There were statistical dift~rences in the rates of the multiple r infection, low - risk multiple infection, high - risk infection, high - risk漳州市自然科学基金科技项目（ZZ2012J21

Expression and clinical significance of RRM1,CDX2 in gastric cancer tissues

目的探讨RRM1、CDX2在胃癌组织中的表达,并分析其与胃癌侵袭与转移的关系。方法选取90例术前未行放化疗的胃癌石蜡标本及与其对应正常胃黏膜蜡标本30例,采用SP免疫组化方法检测RRM1和CDX2在两种组织中表达情况,分析二者表达与分化程度、浸润深度、淋巴结转移、TNM分期之间的关系。结果 （1）RRM1、CDX2在胃癌中的阳性表达率均显著高于正常胃黏膜组（86.7%vs 40.0%,P〈0.01;61.1%vs 16.7%,P〈0.01）。（2）90例胃癌中,RRM1与CDX2二者之间呈正相关关系（r_s=0.223,P=0.034）;RRM1、CDX2表达与分化程度密切相关（P〈0.05）,CDX2表达与浸润深度、淋巴结转移、TNM分期密切相关（P〈0.05）,RRM1表达与浸润深度、淋巴结转移、TNM分期无相关（P〉0.05）。结论 RRM1、CDX2可作为较为理想的肿瘤标志物,用于胃癌的诊断和预后的预警。Objective To explore the expression of RRM1 and CDX2 in gastric cancer tissues and its relationship with invasion and metastasis of gastric cancer. Methods Expression of RRM1 and CDX2 was detected in 90 cases of gastric cancer without preoperative chemoradiotherapy and 30 cases of normal gastric mucosa using immunohistochemistry SP method. The relationships of RRM1 and CDX2 expression with differentiation degree,tumor invasion depth,TNM stage and lymph node metastasis were analyzed. Results（1）The positive expression rates of RRM1 and CDX2 were both significantly higher in gastric cancer tissues than in those of normal gastric mucosa（86. 7% vs 40. 0%,P 〈 0. 01; 61. 1% vs 16. 7%,P 〈 0. 01）.（2） The expression of RRM1 in 90 cases of gastric cancer was positively correlated with CDX2（ r_s= 0. 223,P = 0. 034）. The expression of RRM1 and CDX2 was related with differentiation degree（ P〈 0. 05）. The expression of CDX2 was related with tumor invasion depth,TNM stage and lymph node metastasis（ P 〈 0. 05）. The expression of RRM1 was not related with tumor invasion depth,TNM stage,lymph node metastasis（ P 〉 0. 05）. Conclusion RRM1 and CDX2 can be used as ideal tumor markers in diagnosis of gastric cancer and prognosis.福建省教育厅B类科技基金资助项目（JB12319

On the Discourse of China in Anthropology ——A Recording of The 6th Summit Forum on Anthropology

就“人类学的中国话语”主题，学者们各抒己见，认为中国人类学要有自己对于田野材料的理论提升即“话语权”，就必须做好研究，进行系统的学科建设，以及跨学科整合。Centering on the theme of ＂the Discourse of China in Anthropol- ogy＂, scholars have voiced their own opinions and agreed that China needs to make researches and systematic discipline construction if it were to have its own theories on fieldwork, or discourse power