两个蛇毒基因克隆及cDNA序列多态性再分析

α-银环蛇毒素(α-bungarotoxin)是一种突触后神经毒素,广泛存在于眼镜蛇科蛇类的毒腺中,对于该基因cDNA多态性是否真实一直存有争议。本研究从银环蛇基因组DNA中克隆到α-银环蛇毒素基因序列,并对其中5个克隆进行测序和序列比对分析。作为参照,从同一次反转录得到的cDNA混合物中,克隆了蛇毒神经生长因子cDNA,并对其进行测序、比对和突变情况分析。综合各研究组报道的α-银环蛇毒素cDNA序列、α-银环蛇毒素基因序列和神经生长因子cDNA序列的突变情况,发现α-银环蛇毒素cDNA的多态性在基因组模板上不存在对应的变化,因此推测这种多态性不是从不同的转录本而来,同时考虑到不同研究小组报道的序列突变位点并没有出现相同的情况,因此其多样性也不是RNA编辑的结果。可见这种cDNA序列上的多样性很可能是由反转录过程以及基因克隆过程中人为引入的错误造成的

日本語母語話者教師の判定に基づく発音項目の指導重要度に関する検討 : 中国語を母語とする日本語学習者のために<研究論文>

中国語を母語とする日本語学習者に対する発音指導の重要性を定量的に検討するため，本研究では現役の日本語母語話者教師 34 名を対象に，36 の発音項目についてアンケート調査を行った。各発音項目の指導重要度を「1.まったく重要ではない」から「6.非常に重要である」の 6 段階で評定させた結果，分節音よりも超分節音のほうが，また，分節音では母音よりも子音のほうが高い重要度を示した。一方，超分節音では特殊拍・アクセント・イントネーション・ポーズの各重要度に有意差がなかった。さらに，母音では 4 項目の各重要度に有意差がない一方，子音では子音を分類する基準である調音場所・調音方法・有声無声のいずれも重要度に影響を与えた。アクセントでは，各項目間に重要度の差がみられなかったが，特殊拍では連母音よりも長音と促音のほうが重要度が有意に高かった。イントネーションでは，フォーカスが疑問文の文末および態度のイントネーションより重要度が有意に低かった。以上の結果は，今後の中国語を母語とする日本語学習者を対象とした発音指導にとって，重要な知見となることが期待される。textapplication/pdfdepartmental bulletin pape

The Application of Bioinformatics in the Research of Alternative Splicing

基因选择性剪接现象是真核生物基本而又重要的调控机制。由于基因的选择性剪接在形成生物复杂性和多样性上具有极其重要的作用,同时选择性剪接与许多人类疾病也密切相关。因此,研究基因选择性剪接是一项十分重要的工作。生物信息学作为一门新兴的学科在研究基因选择性剪接上起关键的作用,尤其在研究基因表达调控机制、选择性剪接基因预测以及选择性剪接基因进化上。文章综述了这方面的最新研究进展,为更深入了解真核生物基因的表达调控机理提供依据。Alternative splicing,a fundamental and important regulatory mechanism in eukaryotes,allows one pre-(mRNA) to be processed into many different mature forms within a cell,each of which can have distinct functions.As alternative splicing is associated with human diseases,the study of alternative splicing becomes quite important.Bioinformatics is a new subject for the study of alternative splicing,especially for its regulatory mechanism,prediction and origin.Of course,bioinformatics must be combined with experimental research so as to clarify these aspects of alternative splicing.This paper reviewed the recent research progress in this field in the hope to gain a deeper understanding of eukaryotic gene expression regulation.国家自然科学基金项目(编号:60305001,30470938)资助~

Demand-supply coordination in financial service of Taiwanese invested agricultural enterprises——Taking Taiwan farmers business bases in Fujian as a case

以福建省台资农业企业金融服务为切入点,从金融网点分布的有限性、授信条件的局限性、金融产品服务的单一性以及金融服务意识的被动性等方面,阐述福建省金融服务供给滞后于金融服务需求的情况,进而提出针对性建议,以期加快福建省台资农业企业金融服务的创新。The current status of the financial service supply lagging behind the demand for it in Fujian Province is dealt with in terms of the limit of financial network layout,the limit pf credit extension conditions,the singleness of financial product,and the passivism of financial service awareness,taking the financial service of Taiwanese-invested agricultural enterprises in Fujian Province as a case.The countermeasures are proposed to accelerate the innovation of financial service of Taiwanese-invested agricultural enterprises in Fujian Province

Materials Genome Initiative and Nuclear Fuel Element Material

核能由于其高能量密度和低污染排放等优点,已经成为未来能源的重要组成部分。然而,民用核燃料材料因其特殊的放射性,实验研究的安全防护成本极高,尤其是; 经过辐照后的核燃料材料,分析和表征手段极其有限,如果采用传统的试错法材料研发方法,将会使材料的研发成本大幅提高,因此,材料基因工程的研究思路正是; 适合于新型民用核燃料材料研究的技术路线。本研究组多年来以开发新型民用核燃料元件材料为目标,通过第一性原理和CALPHAD技术的结合,先后建立了U; 、Pu等锕系元素的多组元热力学数据库,并建立了辐照场作用下的热力学模型,对辐照场作用下核燃料材料的相变热力学和动力学进行了深入研究,在热力学数据; 库的基础上,运用相场动力学模型对核燃料元件材料的凝固和时效过程组织演化规律进行了系统的研究。这种基于材料基因工程的多尺度、多组元的材料设计研发思; 路为我国新一代具有自主知识产权的民用核燃料元件材料的成分设计、组织控制、工艺优化、性能改善及服役时间预测提供了重要的理论基础,同时对材料基因工程; 方法在材料开发中的广泛应用具有重要意义。Nuclear energy is an important part of the future source of energy due; to their higher energy density and lower emission of pollutants.; However, the traditional research method of "trial-and-error" may result; in higher costs and lower efficiency because of the radioactivity of the; nuclear fuel element material. The idea of Materials Genome; Initiative(MGI) is suitable for the research and development of the; nuclear fuel element material. Focused on the nuclear fuel element; material,our research group developed a multi-component thermodynamic; database including U,Pu and other elements,by coupling CALPHAD method; and the first-principle method. Based on the thermodynamic database, the; thermodynamic model under irradiation was established and the phase; transformations under irradiation were systematically investigated. The; microstructure evolutions during solidification and aging were simulated; by using the Phase-Field method. The present multiscale and; multi-component materials design method based on MGI can provide; important information for the design of composition,microstructure; controlling and property improvement of nuclear fuels materials.中央高校基本科研业务费; 国家自然科学基金资助项

Expression and functional analysis of recombinant α-bungarotoxin (P22-A31)

α-银环蛇毒素在神经科学研究以及在临床和制药上有重要作用,为获得大量的α-银环蛇毒素,我们用已构建的pGEX-BgTX(P22-A31)质粒在大肠杆菌BL21(DE3)中表达,并用谷胱甘肽Sepharose FF纯化GST-α-银环蛇毒素融合蛋白,再用凝血酶切掉融合标签谷胱苷肽转移酶(Glutathione S-transferase,GST),得到了较纯的重组α-银环蛇毒素同工毒素,得率约为1.225mg/L。用重组α-银环蛇毒素制备多克隆抗体,经ELISA和Western杂交鉴定后可知重组α-银环蛇毒素与天然α-银环蛇毒素的抗原性一致。小鼠毒性试验表明,重组α-银环蛇毒素同工毒素的半致死剂量LD50为1.598mg/kg,约为天然α-银环蛇毒素的1/5。小鼠化学法镇痛试验显示,重组α-银环蛇毒素有一定的镇痛药效,1/4LD50剂量的镇痛百分率为55.2%,1/8LD50剂量的镇痛百分率为20.5%,在外周镇痛作用中呈一定的量效关系。α-bungarotoxin plays very important role in neuroscience research, clinical application and the pharmaceutical industry. In order to acquire quantitites of α-bungarotoxin, we expressed GST-α-bungarotoxin fusion protein using constructed plasmid pGEX-BgTX (P22-A31) in E.coli BL21 (DE3)cell, purified GST-α-bungarotoxin using glutathione-Sepharose FF, cleavaged Glutathione S-transferase(GST) tag by thrombin in the column, and finally, we obtained recombinant α-bungarotoxin with yields of about 1.225 mg/L. In order to compare the immunogenicity of recombinant protein and crude α-bungarotoxin, we prepared multi-antibody using recombinant α-bungarotoxin. The results of both ELISA and Western blot showed that recombinant α-bungarotoxin has the same antigenicity as natural α-bungarotoxin. In vivo toxicity tests showed that the LD_ 50 of recombinant α-bungarotoxin was 1.598 mg/kg, about 1/5 that of natural α-bungarotoxin. Analgesis percentages with doses of 1/4 LD_ 50 and 1/8 LD_ 50 were 55.2% and 20.5% respectively, indicating that recombinant α-bungarotoxin possesses analgesic efficiency.国家自然科学基金(No.30470938);; 教育部留学回国人员启动基金资助~

Research and development on life ball system for mine disaster rescue

设计了一个矿难救险设施。介绍了生命球的系统组成、结构、受力分析及仿真和生命球与地面连通的整体系统。通过相关的仿真模拟,证明了该设计结构的合理性和可行性。福建省自然科学基金资助项目(Z0511011

Determination of Iron in Coastal Zone Water with Improved National Standard Method

为了更加准确地检测海岸带水体中的铁含量,将邻菲啰啉分光光度法测定水质铁(国标法)的方法进行了改进。在邻菲啰啉分光光度法的基础上,在p H调节方式、比色皿皿差和显色剂三个方面进行改进。结果表明,改进后方法的线性、准确度和精密度都优于国标法。但改变p H调节方式和扣除比色皿皿差后检测低浓度铁的水样的精密度依然较差(RSD&gt;5%),说明邻菲啰啉的灵敏度较低。以5-Br-PADAP为显色剂用分光光度法检测水样时,方法的准确度和精密度较好(RSD&lt;5%,加标回收率在96.0%~106%之间),该方法可以用于海岸带水体中铁的检测

Synthesis and cellular uptake mechanism of Tat decorated Au-Au_2S nanoparticles

［中文文摘］为了实现对肿瘤的靶向性药物/基因治疗,通过化学还原法制备了细胞穿膜肽Tat修饰的Au-Au2S纳米药物载体。采用透射电镜、表面增强拉曼光谱仪、紫外分光光度计对Tat/Au-Au2S纳米粒子进行表征,采用流式细胞仪、激光共聚焦显微镜研究Tat/Au-Au2S纳米粒子的穿细胞膜机制。理化分析结果表明,Tat可通过Au—S键接枝于Au-Au2S纳米粒子表面,直径约50 nm的Tat/Au-Au2S纳米粒子具有近红外敏感性。细胞内化途径示踪物共定位分析和抑制剂阻断实验表明,Tat/Au-Au2S纳米粒子以脂筏介导的巨胞饮途径进入Hela细胞,而以受体和脂筏共介导的巨胞饮途径进入骨髓间充质干细胞(BMSCs)。［英文文摘］　To realize an efficient and targeted cancer therapy by the drug/ gene carrier , cell penet rating peptide Tat decorated Au2Au2 S nanoparticles were prepared by a redox method. Transmission elect ron microscopy ( TEM) ,surface2enhanced Raman scattering ( SERS) and UV2vis spect rometer were used for characterizing Tat/ Au2Au2 S nanoparticles , and confocal laser scanning microscope (CLSM) and flow cytometer (FACS) were used to investigate the mechanism of cellular uptake. The chemicophysical result s indicate that Tat peptide could be conjugated onto Au2Au2 S nanoparticles via Au —S bonds , and Tat/ Au2Au2 S nanoparticles present as 50 nm2 diameter sphericities with NIR sensitivity. Co 2location and endocytosis inhibition experiment s suggest that Tat/ Au2Au2 S nanoparticles may enter Hela cells via a lipid raf t mediated endocytosis pathway , whereas via a combined endocytosis pathway of lipid raf t2dependent and receptor2dependent into bone marrow st romal cells (BMSCs).973计划项目(2007CB935603); 国家自然科学基金(30670559);福建省自然科学基金(2006J0121);福建省新世纪优秀人才计划项目

低温催化裂解烷烃法制备碳纳米管

低温催化裂解烷烃法制备碳纳米管陈萍，王培峰，林国栋，张鸿斌，蔡启瑞（厦门大学化学系固体表面物理化学国家重点实验室，厦门，３６１００５）关键词碳纳米管，催化裂解，甲烷碳纳米管的制备与研究是国际上新材料领域的探索热点［１］．由于具有纳米级的管径，碳纳米管..