闽南肝癌高发区肝细胞癌与HBV复制的相关性分析

目的分析闽南肝癌高发区乙型肝炎病毒 (HBV)复制与原发性肝细胞癌 (PHCC)的关系。方法用实时荧光定量聚合酶链反应 (PQ- PCR)技术测定 6 1例 PHCC患者、40 7例不同病程的 HBV感染者及 17例健康人血清中 HBV DNA的含量 ,对照分析 HBV标志物 (HBVM) ,同时检测 PHCC患者抗 - HCV- Ig G和 HCV RNA。结果PHCC组 HBV DNA阳性率高达 80 .3% (49/ 6 1) ,高于其他肝病组 ,差异具显著性 (P<0 .0 2 ) ,HBV DNA含量各组间差异无显著性。PHCC组抗 - HCV- Ig G和 HVC RNA阳性率为 0。结论闽南肝癌高发区 PHCC患者 HBV DNA阳性率较高 ,HBV感染并持续复制 ,可能是该地区 PHCC的主要致病因

Hypoglycemic action of acidic polysaccharide in leaf of Gynura divaricata and its effect on related indexes

与西药相比,传统中药及其提取物具有经济、安全的优点,同时还可避免西药的许多副作用[1]。中药材白子草又名白背三七,为菊科(COMPOSITAE)三七属(gynurA CASS.)植物白子菜〔gynurA dIVArICATA(lInn.)dC.〕的干燥叶,具有清热、舒筋、止血、祛痰等功效[2]。在民间,白子菜的茎和叶可药食两用,用于治疗高血压、糖尿病和高血脂症等[3],其化学成分复杂,目前已分离获得多糖类[3]、黄酮类[4-6]、生物碱[4,7-8]和脂肪酸[9]等成分。Gynura divaricata acidic polysaccharide( GDAP) was extracted and purified from dry leaf of Gynura divaricata( Linn.) DC.Effects of successive lavage GDAP for 0,7,14 and 21 d with daily dose of 15,37 and 60 mg·kg- 1on blood glucose content of diabetic model mice were analyzed,and differences in SOD activity and MDA content in serum and glycogen content in liver of mice were compared.The results show that with prolonging of successive administration time and increasing of GDAP daily dose,blood glucose content of diabetic model mice generally appears the decreasing trend.With successive administration for 14 and 21 d,blood glucose content of different GDAP treatment groups is generally lower than that of CK group( 0.9% Na Cl injection with daily dose of 10 m L·kg- 1) but higher than that of T1group( metformin with daily dose of 200 mg·kg- 1).In which,after successive administration GDAP for 21 d with daily dose of 37 and60 mg·kg- 1,blood glucose content of diabetic model mice is significantly or extremely significantly lower than that of CK group but significantly or extremely significantly higher than that of T1 group,while SOD activity in serum of diabetic model mice is significantly higher and its MDA content is significantly lower than those of CK group,but there is no significant difference with those of T1 group.Glycogen content in liver of diabetic model mice of different GDAP treatment groups is higher than that of CK group,but lower than that of T1 group.It is suggested that GDAP is the main hypoglycemic components in polysaccharide of G.divaricata leaf, and it has dose-effect relationship in hypoglycemic activity.Hypoglycemic mechanism of GDAP is probably related to improving antioxidant enzyme ability in the body,enhancing activity of scavenging oxygen free radicals,relieving liver damage,and increasing glycogen storage capacity.福建省自然科学基金资助项目(2012J01400

Spatial niches of dominant zooplankton species in Sanmen Bay,Zhejiang Province of East China

生态位与种间竞争、资源利用密切联系,体现了物种在群落中利用资源的能力.为探明三门湾浮游动物分布格局的形成和影响因素,分别采用SHAnnOn公式和PETrAIlIS指数测定了浮游动物优势种生态位宽度和生态位重叠,并通过典范对应分析研究生态位分化状况.结果表明:百陶带箭虫、背针胸刺水蚤、短尾类幼虫等沿岸种的空间生态位较宽,而肥胖软箭虫、中华假磷虾等外海种的空间生态位较窄;具有捕食-被捕食关系的不同类群物种(如箭虫、仔鱼和水母分别与桡足类)有较高的生态位重叠,而同一类群物种(如桡足类及箭虫)间的生态位重叠值较低;浮游动物分布受温度、盐度和叶绿素A的影响较大,受营养盐影响较小.浮游动物空间生态位与生活类型、种间竞争、摄食等多种因素相关.Ecological niche has close relations with inter-species competition and resources utilization,and thus,can be used as an indicator to symbolize the resources utilization capability of certain groups of related species.In this paper,the niche breadth and overlap of the dominant zooplankton species in the Sanmen Bay of Zhejiang were determined by Shannon' s formula and Petraitis index,respectively,and the differentiation of the niche was studied by canonical correspondence analysis(CCA),aiming to approach the distribution features of coastal zooplankton and related affecting factors in the Bay.In the study area,some coastal species such as Zonosagitta bedoti,Centropages dorsispinatus,and brachyuran larvae had wider niche breadth than the pelagic species such as Flaccisagitta enflata and Pseudeuphausia sinica,and the species of different groups with prey-predator relation had a greater niche overlap than those of the same groups without this relation.The CCA analysis showed that the spatial distribution of the zooplankton was more affected by water temperature,salinity,and chlorophyll a content rather than nutrients.The spatial niche of the zooplankton species was correlated with their living habit,inter-species competition,and prey-predator relationship.国家重点基础研究发展计划项目(2010CB428903); 海洋公益性行业科研专项(201305043-3); 浙江省自然科学基金项目(Y5110166); 浙江省海水养殖重点科技创新团队项目(2010R50025); 国家海洋局第二海洋研究所基本科研业务费专项(JG1221;JG1222)资

Epidemiological Analysis of Endemic Fluorosis in Hebei Province

Endemic fluorosis is prevalent in many areas of Hebei Province, China, and represents a considerable health hazard for the local population. An investigation was accordingly conducted from 1980 to 1982 to determine the fluoride concentration in the drinking water, soil, crops, and atmosphere, as well as the fluoride level in human urine and the prevalence of mottled enamel and osteosclerosis, Patients with endemic fluorosis numbered 5,672,539 persons living in 126 cities, 1,200 communes, and 10,727 villages throughout the province. These patients represent 10% of the total resident population. The high incidence areas divide geographically into plateaus, mountains, basins, plains, and coastal regions, Most of the sources of drinking water showing high fluoride levels were shallow wells, although some deep wells, springs, and rivers were similarly contaminated. Fluoride concentrations were generally higher in the northern part of the province and lower in the south. No significant relation obtained between the prevalence of fluorosis and the fluoride levels in grain and vegetable crops or the atmosphere. Although atmospheric fluoride levels were higher in areas of high prevalence of fluorosis, its source was attributed to smoke from coal burned as fuel. The prevalence mottled teeth was found to increase sharply when the fluoride level in the drinking water rose above. 1.0 mg/1, and accounted for 100% of the population when the fluoride level was greater than 4.0 mg/1. In patients from areas of relatively low concentrations, the tooth enamel showed an opaque whiteness. Patients from areas of high concentrations exhibited pitted enamel. Osteosclerosis was also found to increase in severity with higher concentrations of fluoride in the drinking water. As result of this investigation, we conclude that endemic fluorosis in Hebei province is caused by high fluoride in the drinking water. Accordingly, lowering the fluoride concentra-tion in drinking water is the primary measure for preventing this condition

Spectrum-effect relationship of immunosuppressive effects and toxicity of Tripterygium wilfordii HooK F.

目的:通过谱效分析探讨雷公藤药材提取物高效液相色谱图中免疫抑制活性及肾毒性相关色谱峰,为确定雷公藤免疫抑制活性及毒性物质基础提供依据。方法:对8个产地雷公藤药材分别进行提取纯化,采用高效液相色谱法(HPLC)建立各产地雷公藤提取物的指纹图谱。利用密度梯度离心法分离新鲜人外周血单个核细胞(PBMC),给予凝集素(PHA)和脂多糖(LPS)共刺激造模,分为空白组、模型组(PHA+LPS)、雷公藤处理组。给予雷公藤处理48 h后检测上清中IFN-γ和IL-2的含量,并计算IC50值。以小鼠肾小球系膜细胞(GNM-SV40)为模型利用CCK8法考察雷公藤的肾毒性,计算IC50值。将各产地雷公藤的指纹图谱信息及免疫抑制药效、细胞毒性数据进行标准化处理后利用偏最小二乘法进行谱效关系分析。结果:免疫抑制药效试验中,雷公藤提取物可显著抑制PHA和LPS诱导PBMC免疫细胞因子IFN-γ和IL-2的分泌(P0.3);当以IL-2 IC50值为毒性指标时,1,8,3,5号峰为主要贡献峰(回归系数绝对值>0.3),16~29号峰为次要贡献峰(回归系数绝对值0.3),15~29号峰为次要贡献峰(回归系数绝对值0.3);peak 1,8,5 and3 contributed greatly to decrease of IL-2(absolute value of coefficient>0.3);peak1,8 and3 contributed greatly to toxicity on GNM-SV40 cells(absolute value of coefficient>0.3).CONCLUSION Immunosuppressive activity and toxicity of TWHF have the closest correlation with peak1,3 and 8in HPLC fingerprints.Peak2 and 5 are main effective peaks without toxicity.Peak 15-29 have certain effects on immunosuppressive activity and toxicity,but not significant.福建省自然科学基金项目(编号:2012I1001

Research progress of toxicity mechanism of Tripterygium wilfordii

目的:介绍近年来对雷公藤毒性作用机制的研究进展,探讨雷公藤成分与毒性的关系,为临床合理使用雷公藤及其制剂提供指导。方法:系统查阅近年来的国内外文献,综述关于雷公藤毒性作用机制的研究进展情况。结果:雷公藤毒性涉及多器官多系统,其毒性成分同时也是药效成分,雷公藤毒性作用机制的研究对雷公藤高效低毒制剂研发有指导意义,并为指导临床合理用药提供理论依据。结论:雷公藤毒性作用机制的研究已逐渐成为雷公藤研究的热点并取得了很多进展,但由于化学成分的复杂性及中药药理的整体性目前关于雷公藤毒性作用机制的研究还不够完善和系统,尚需进一步研究。OBJECTIVE To update recent progress in toxic mechanism of Tripterygium wilfordii,discuss relationship between its toxicity and constituents,and provide a guidance for rational drug use in clinical practice.METHODS By referring to recent literatures at home and abroad systematically,toxic mechanism of Tripterygium wilfordii was reviewed.RESULTSToxicity of tripterygium wilfordii was involved in multiple organs and systems,and toxic components were also considered as effective components.Research on toxic mechanism of Tripterygium wilfordii is helpful to develop preparations of high efficiency and low toxicity,and provide a guidance for rational drug use in clinical practice.CONCLUSION Toxic mechanism of Tripterygium wilfordii has become a focus,and some progresses have been achieved.However,studies on acion mechanism are still not complete and systemic for Tripterygium wilfordii due to complexity of its chemical components and integrity of TCM pharmacology,and further studies are still needed.福建省科技计划重大项目(编号:2012I1001

Seasonal variations of zooplankton community distribution and environmental impact factors in Yueqing Bay

2006年10月、2007年1月、4月及7月对乐清湾海域浮游动物群落的种类组成、优势种、生态类群、水平分布的季节性变化特征进行了调查,分析了浮游动物群落分布与环境因子的关系。结果表明,在调查海域共鉴定出浮游动物82种(包括浮游幼体11种),隶属于15大类,其中秋季为46种、夏季为42种、春季为25种和冬季为16种。乐清湾浮游动物可分为近岸低盐类群、暖水性近海类群、暖温带近海类群和暖水性广布类群4个生态类群,其中近岸低盐类群在全年均占优势,其它类群则呈现明显的季节变化。中华哲水蚤CAlAnuS SInICuS、真刺唇角水蚤lAbIdOCErA EuCHAETA、针刺拟哲水蚤PArACAlAnuS ACulEATuS和背针胸刺水蚤CEnTrOPAgES dOrSISPInATuS为调查海域主要优势种。浮游动物生物量年平均值为82.7Mg/M3,其大小依序为:夏季(121.1Mg/M3)>秋季(119.2Mg/M3)>春季(48.5Mg/M3)>冬季(42.2Mg/M3);丰度年平均值为82.1个/M3,其大小依序为:夏季(193.4个/M3)>秋季(73.7个/M3)>春季(53.4个/M3)>冬季(9.8个/M3)。相同季节浮游动物生物量和丰度的平面分布趋势类似,季节间则存在明显差异。相关性分析结果表明,浮游动物物种数与水温、盐度、叶绿素A质量浓度和浮游植物细胞密度均呈极显著相关;丰度与水温、叶绿素A质量浓度和浮游植物细胞密度呈极显著相关;生物量与水温、叶绿素A质量浓度呈极显著相关。与历史资料相比,近30A来浮游动物数量呈下降趋势,但群落结构和组成没有发生明显改变。The community composition,dominant species,ecological groups and plane distribution of zooplankton in Yueqing Bay were investigated in October 2006,January,April and July 2007 at 18 stations and the relationships between the community distribution and environmental factors were analyzed.Totally 82 species of 15 major categories have been identified,including 25 species in spring,42 in summer,46 in autumn and 16 in winter,which can be divided into 4 ecological groups of coastal low salinity species,estuarine brackish water species,coastal warm water species and warm water eurytopic species.The coastal low salinity species is always dominant throughout the year,while the others have distinct seasonal variations.The main dominant species in the study area are Calanus sinicus,Labidorcera euchaeta,Paracalanus aculeatus and Centropages dorsispinatus.The annual average biomass of zooplankton is 82.7 mg/m3,and summer has the largest value(121.1 mg/m3),next is autumn(119.2 mg/m3),spring(48.5 mg/m3) and winter(42.2 mg/m3).The annual average abundance is 82.1 ind/m3,with summer(193.4 ind/m3)being the highest,followed by autumn(73.7 ind/m3),spring(53.4 ind/m3)and winter(9.8 ind/m3).The patterns of horizontal distribution of biomass and abundance are similar in same season and have distinct differences in different seasons.Studies show that the species number of zooplankton relates closely to water temperature,salinity,mass concentration of chlorophyll a and phytoplankton cell density,abundance relates to water temperature,mass concentration of chlorophyll a and phytoplankton cell density,while biomass primarily to water temperature and mass concentration of chlorophyll a.Compared with the historical data,there are no considerable changes for dominant species and ecological groups in Yueqing Bay since 1980s,but the zooplankton biomass and abundance has sharply reduced.“973”计划资助项目(2010CB428903);国家海洋公益性行业科研专项经费资助项目(200805069);国家海洋局第二海洋研究所基本科研业务费专项资助项目(JT0806

Isolation,purification and identification of polysaccharides from Gynura divaricata

对白子草中的多糖进行分离纯化,并对得到的多糖组分的理化性质进行分析。将脱脂后的白子草药材经热水提取,乙醇沉淀,木瓜蛋白酶-SEVAg法去蛋白后,再经dEAE-SEPHArOSE fAST flOW离子交换柱色谱分离,透析除盐纯化得白子草多糖,采用高效液相色谱法,红外光谱和~1H-nMr对其进行组成研究和分析。白子草多糖中的gdPS-2的纯度为87.3%,相对分子质量为2.03x10~4 dA;gdPS-3的纯度为90.9%,相对分子质量为4.29x10~4dA。红外光谱无法确定gdPS-2是否有α构型的呋喃糖苷,但提示gdPS-3可能有α构型的呋喃糖苷键,~1H-nMr分析初步判断gdPS-2和gdPS-3的糖苷键连接方式均为α型。gdPS-2由葡萄糖醛酸和木糖组成,摩尔比为1.1:0.63;gdPS-3由鼠李糖、葡萄糖醛酸、半乳糖、木糖和半乳糖醛酸组成,摩尔比为0.32:6.0:0.21:1.75:4.3。The purpose of this study was to isolate and purify polysaccharide from Gynuia divancaia and analyze its monosaccharide composition.A water-soluble crude polysaccharide was obtained by hot water extraction,ethanol precipitation and deproteinization after degreasing.The crude polysaccharide then purified with DEAE-Sepharose Fast Flow column chromatography and dialysis.The monosaccharide composition and structure were analyzed by HPLC,UV spectrophotometer and H-NMR.The results showed that the purity and molecular weight of GDPS-2 and GDPS-3 were 87.3%,2.03×10~4 Da and 90.9%,4.29×10~4 Da,respectively.The UV spectrophotometer and ~1H-NMR data suggested that glycosidic bond of GDPS-2 and GDPS-3 were α type.Both GDPs-2 and GDPs-3 were homogeneous polysaccharides,and GDPs-2 was mainly composed of glucuronic acid and xylose at a molar ratio of 1.1:0.63.GDPs-3 was mainly composed of rhamnose,glucuronic acid,galactose,xylose and galacturonic acid at a molar ratio of 0.32:6.0:0.21:1.75:4.3.福建省自然科学基金项目(2012J01400