多校区建设中的若干问题分析——国家教育行政学院第32期高校领导干部进修班赴西安考察报告

【中文摘要】 2008年3月14-16日,国家教育行政学院第32期高校领导干部进修班全体学员赴西安考察了长安大学等四所高校。考察围绕多校区校园建设主题展开,重点就多校区管理模式、多校区建设与校园文化、多校区建设中的债务问题、新校区运行成本与效益等四个方面的问题进行了深入分析,并提出了相应的对策。 更多还

Separation of terpenes and oxygenated compounds from citrus oil by supercritical CO_2 extraction

以冷榨柑橘精油为原料,采用gC/MS对柑橘精油原料进行定性及定量分析,确定了柑橘精油中的7种萜烯类化合物成分作为分离考察对象。实验探讨了超临界萃取压力、萃取温度、萃取时间和CO2流量等因素对含氧化合物分离效果的影响。实验结果表明,萃取相中萜烯类化合物的回收率总体上随着萃取压力、温度、时间和CO2流量的增大而增大。当萃取压力为12MPA,萃取温度为45℃,萃取时间为4H以及CO2流量为1.0l/MIn时,分离效果最佳,其萃取相中萜烯类化合物的回收率高达90.03%。Supercritical CO2 extraction was employed to separate terpenes and oxygenated compounds from citrus oil.The raw oil,the extract,and the raffinate were qualitatively and quantitatively analyzed by GC/MS,respectively,with the terpenes being represented by seven typical compounds.The influences of several experimental factors including extration pressure,extraction temperature,extraction time and CO2 flow rate on the separation of the oxygenated compounds were studied.The results indicate that the recovery rate of terpenes in the extract generally increases with the increasing of extraction pressure,temperature,time and CO2 flow rate.The optimum conditions for extracting terpenes are as follows: pressure 12 MPa,temperature 45 ℃,extraction time 4 h,and CO2 flow rate 1.0 L/min.Under these optimum conditions,the recovery rate of terpenes in the extract is 90.03%.福建省新世纪人才支持项目(0000-X04157

赤潮生物毒素的药理作用研究进展

20 0 4年度厦门市科技创新基金项目 ;; 2 0 0 4年福建省海洋与渔业局科技项目 ;; 国家自然科学基金 (40 0 760 31 ;40 3760 32 )资

以课题式实验教学提高学生科研兴趣和科研能力的探索——以“遗传学实验”为例

传统的实验教学多为验证性实验,实验结果可预测且大同小异,缺乏新意和挑战,对于拔尖学生来说如同鸡肋,因此在经典传统的实验教学中进行内容改革,提高难度势在必行。本文以遗传学实验中的经典实验模式生物果蝇为例,开展了课题式实验教学改革,以果蝇诱变为命题,大大激发了学生的科研兴趣,同时拓展了科学思维,培养了科研能力,体现了研究性学习的创造性和挑战性,是生物学实验教学改革的有益探索。“基础学科拔尖学生培养计划”研究课题(20180214,20170606

p38MAPK induces apoptosis of glioma cell

目的　研究 p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响 .方法　利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系 C6中 ,用免疫细胞化学染色检测其在细胞转染前后的表达情况 ,用 HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响 .结果　转染 p CMV5 - p38MAPK质粒组 p38MAPK蛋白表达阳性 ,细胞形态发生变化 ,贴壁性降低 ,出现大量凋亡细胞 .结论　转染 p38MAPK基因可诱导胶质瘤细胞凋

Impact of p38MAPK and RGS16 to the apoptosis and cell cycle of the glioma C6 cells

目的　探讨p38MAPK和RGS16对大鼠胶质瘤C6细胞的生物学特性的影响 .方法　利用脂质体介导法将p38MAPK和RGS16基因分别或共转染导入C6细胞中 ;用p38MAPK抑制剂SB 2 0 2 190处理处于对数生长期的转染和未转染 p38MAPK的C6细胞 ,2 4～ 36h后在倒置显微镜下观察细胞形态变化和贴壁情况 ;免疫细胞化学法检测转染前后p38MAPK和RGS16蛋白的表达情况 ;流式细胞仪检测细胞周期变化和细胞是否有凋亡发生 .结果　转染 pCMV5 p38和 /或pCMV5 RGS16质粒 36h后 30 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ;p38MAPK和RGS16蛋白均表达阳性 ;转染pCMV5 p38组出现 33.8%的凋亡峰 ,细胞周期结果显示G1期细胞百分数增加 17% ,而S期细胞百分数减少 14 % ;转染pCMV5 RGS16组无凋亡发生 ,细胞周期结果显示G1期细胞百分数减少 10 % ,而S期细胞百分数增加 14 % ;共转染pCMV5 P38和pCMV5 RGS16组未出现的凋亡峰 ,细胞周期结果显示G1期和S期细胞百分数变化与未处理组之间没有明... 【英文摘要】 AIM To study the effects of p38MAPK and RGS16 on the biological characteristics of glioma C6 cells. METHODS pCMV5 p38 and pCMV5 RGS16 were respectively or jointly transfected into C6 cells by lipofectin. SB 202190 was used to treat the transfected and nontransfected pCMV5 p38 C6 cells. The morphological and adhesive changes of the cells were observed under an inverted microscope. Expression of p38 and RGS16 was examined by immunocytochemical method both before and after the transfection. Flow Cytometr...高等学校骨干教师资助计划项目 ;; 留学归国人员科研启动基金项目 ([1 999] 747号

Separation and identification of auraptene from grapefruit peel oil

橙油素是广泛存在于柑橘类果实中的天然抗癌活性成分。选用SP70大孔吸附树脂分离纯化葡萄柚精油中的橙油素,该树脂对于橙油素的吸附容量和解吸率分别达到14.53 Mg/g和83.32%,并成功地从树脂床层的洗脱液中结晶分离出橙油素晶体。对所得晶体分别采用差示扫描量热仪(dSC)、紫外吸收光谱(uV)、红外吸收光谱(Ir)、电喷雾质谱(ESI-MS)进行定性分析,分析结果与橙油素标准品相符合。进一步采用高效液相色谱(HPlC)对所得晶体进行定量分析,结果表明所得晶体中橙油素的质量分数可达85%。Auraptene exists widely in the peels and juice sacs of citrus species and is reported to be an effective inhibitor of chemical carcinogenesis in some rodent models.The separation and purification of auraptene from the grapefruit peel oil was performed by SP70 macroporous resin adsorption.The amount adsorbed and the recovery of auraptene were 14.53 mg/g and 83.32% respectively.Auraptene was crystallized out from the concentrated eluate of macroporous resin bed successfully.Differential scanning calorimetric(DSC),ultraviolet spectrum(UV),infrared spectroscopy(IR) and electrospray ionization mass spectroscopy(ESI-MS) were all used to analyze the obtained crystals qualitatively.The analytical results are in accordance with that of auraptene standard.Furthermore,high performance liquid chromatography(HPLC) was utilized to quantify auraptene of the crystals obtained.The mass fraction of auraptene in the obtained crystals can reach 85%.福建省新世纪人才支持项目(0000-X04157

什么是好的教育政策研究

为进一步加强教育政策研究,更好服务新时代中国特色社会主义教育事业发展,在教育部-上海市人民政府共建的教育经济宏观政策研究院和华东师范大学国家宏观教育政策研究院的支持下,我们特别邀请全国知名教育政策研究专家围绕\"什么是好的教育政策研究\"展开笔谈。笔谈按作者姓氏笔画排序。华东师范大学国家宏观教育政策研究院委托项目“学界有影响力研究成果评判

p38MAPK induces apoptosis of glioma cell

目的　研究 p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响 .方法　利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系 C6中 ,用免疫细胞化学染色检测其在细胞转染前后的表达情况 ,用 HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响 .结果　转染 p CMV5 - p38MAPK质粒组 p38MAPK蛋白表达阳性 ,细胞形态发生变化 ,贴壁性降低 ,出现大量凋亡细胞 .结论　转染 p38MAPK基因可诱导胶质瘤细胞凋亡 【英文摘要】 AIM To study the effect of p38MAPK transfection on the biological characteristics of glioma cell C6. METHODS p38MAPK was transfected into glioma cell C6 by lipofectin. Expression of p38MAPK was detected by immunocytochemistry. HE staining and flow cytometry were adopted to measure the cell morphology, adhesion and cell cycle. RESULTS p38MAPK was expressed in transfected glioma cells, with cell biological characteristics changing and apoptotic cells emerging. CONCLUSION Apoptosis of glioma cell could be ...高等学校骨干教师计划资

Effects of transfected HSP70 on p38MAPK signal pathway

目的　探讨热休克蛋白 (HSP70 )在人胶质瘤细胞BT 32 5 p38MAPK信号通路中的作用。方法　用脂质体介导法将hsp70基因导入人胶质瘤细胞BT 32 5中 ,倒置显微镜观察转染细胞的形态学及粘附性变化 ,紫外线照射 30min后 ,采用免疫组化和Western blot方法测定转染前后HSP70的表达水平及照射前后 p38MAPK表达情况。结果　免疫组化和Western blot证实hsp70基因成功转染入BT 32 5中 ,转染细胞受到紫外线照射后 p38MAPK表达减弱。结论　体外转染hsp70基因可抑制紫外线照射后BT 32 5细胞 p38MAPK的表达 【英文摘要】 Objective To study the role of HSP70 in p38MAPK signal transduction of human glioma cells BT 325.Methods pBBS212 hsp70 gene was transfected into BT 325 cells by lipofectin. The morphological and adhesive changes of the cells were observed under an inverted microscope. The level of HSP70 was measured by immunohistochemistry. Then the transfected cells were put into ultraviolet (UV) for 30 minitues, and expression of p38MAPK and HSP70 were examined by immunohistochemistry and Western blot methods bo...国家自然科学基金资助项目 (30 1 0 0 2 1 8);; 高等学校骨干教师资助计划 (2 0 0 0 - 65 - 66);; 留学归国人员科研启动基金资助项目 (1 999- 747