Progress of interfacial geochemistry of mercury

国家自然科学基金资助项目(20077016);; 中国博士后基金资助项目(2002032148

基于探针内嵌超分子囊泡的半胱氨酸荧光传感

高结构相似度同系物中特定化合物的检测往往需要复杂的分析流程,采用模块化组装的策略制备了一类嵌含光学分子探针的超分子囊泡,在此基础建立了一种集分离与检测功能为一体的新荧光传感模式,提供了解决该问题的一种新思路。将生物硫醇的广普性探针四氟对苯二甲腈嵌入由四(戊硫代)四硫富瓦烯与十二烷基-β-D-麦芽糖苷构成的超分子囊泡膜层后,囊泡对半胱氨酸展现出高选择性、宽动态范围的荧光响应,实现了半胱氨酸的便捷、灵敏检测。由四烃基四硫富瓦烯和表面活性剂共组装形成的空白囊泡制备简单、光谱背景低,可作为构建此类荧光传感体系的通用底版,为这种新传感模式的推广提供有力的支撑。国家自然科学基金(21775129,21475111

基于探针内嵌超分子囊泡的半胱氨酸荧光传感

针对高结构相似度同系物中特定化合物的检测分析流程复杂的问题,采用模块化组装的策略制备了一类嵌含光学分子探针的超分子囊泡,并在此基础上建立了一种集分离与检测功能于一体的新型荧光传感模式.将生物硫醇的广普性探针四氟对苯二甲腈嵌入由四(戊硫代)四硫富瓦烯与十二烷基-β-D-麦芽糖苷构成的超分子囊泡膜层后,囊泡对半胱氨酸展现出高选择性、宽动态范围的荧光响应,实现了半胱氨酸的便捷、灵敏检测.由四烃基四硫富瓦烯和表面活性剂共组装形成的空白囊泡制备简单,光谱可见光区背景吸收低,可作为构建此类荧光传感体系的通用模板,为这种新型传感模式的推广提供有力的支撑.国家自然科学基金(21775129,21475111

Analysis of recombinant human endostatin injection off label use in 120 inpatients in our hospital

目的:分析某院住院患者恩度用药医嘱的合理性,促进临床合理用药。方法:收集2016年1月-2016年12月期间出院的住院患者的恩度用药医嘱,将患者诊断、用法用量与恩度药品说明书进行对比分析,统计超药品说明书情况。结果:120例使用恩度的患者中,男性多于女性,超说明书适应证医嘱24条,占20.00%,超说明书用法用量医嘱117条,占97.50%;有1例发生不良反应,表现为发热。结论:恩度超药品说明书较为普遍,需要设计合理、大样本的随机对照双盲研究提供依据支持

Study on Existent Form of Mercury in Sediments of Miyun Reservoir,Beijng

在改进Tessier方法的基础上,利用冷原子吸收对北京密云水库沉积物中汞的形态进行研究,发现密云水库沉积物的总汞含量较高,沉积物中的汞主要以气态汞、有机汞和硫化物汞的形式存在,生物可以直接利用的汞较低,但一些目前不能直接利用的汞在一定条件下可以转化为可利用的汞,需要对此加以关注。On the bases of modified Tessier method,the existent forms of mercury in sediments of Miyun reservoir,Beijing were determined with cold vapor atomic absorption(CVAA).The results showed that total mercury in the sediments is high,and that gaseous mercury,organic mercury and sulfide mercury(residual mercury) are the main form of mercury.Most of them can not be used by biomass,but,what should be noted is that some unusable mercury can be transformed usable mercury under special conditions..国家自然科学基金(20077016);; 中国博士后基金(2002032148);; 厦门大学行动计划联合资助项

数字图书馆的智能检索技术

信息检索是数字图书馆建设的关健技术之一, 将人工智能新技术与信息检索技术相结合, 设计一种基于 本体论的智能检索工具, 它可更准确、全面地满足用户检索信息的目的

因特网与数字图书馆

因特网与数字图书馆有联系也有区别, 本文从概念、功能和体系结构等方面对这两个概念加以分析, 得出 结论是因特网是数字图书馆的基础设施, 数字图书馆的建设进一步丰富因特网的信息资源

白介素-18在ANCA相关性小血管炎肾损害患者中的表达及其临床意义

目的通过检测抗中性粒细胞胞浆抗体（ANCA）相关性小血管炎（AASV）肾损害患者血清及尿液白介素-18（IL-18）水平,探讨IL-18在AASV肾损害应用中的可能价值。方法比较健康对照组与AASV组、AASV活动组与缓解组间血/尿IL-18水平,分析尿IL-18与传统病情活动性指标如ANCA滴度、血肌酐、C-反应蛋白（CRP）、伯明翰系统性血管炎活动度评分量表（BVAS）评分值等的相关性,绘制尿IL-18评估AASV活动性的ROC曲线,评价IL-18在AASV肾损害中的意义。结果AASV组尿IL-18水平比健康对照组显著升高（P<0.05）,AASV活动组尿液与血液IL-18水平均高于缓解组（P<0.05）。治疗随访半年后,病情由活动转为缓解的患者尿IL-18水平较治疗前明显下降（P<0.05）。AASV患者尿IL-18与血清ANCA滴度、BVAS评分值呈正相关（P<0.05）,与血肌酐、胱抑素C、CRP、尿微量白蛋白/肌酐比值、尿β2-微球蛋白等无明显线性相关。AASV活动期患者的血、尿IL-18水平呈正相关（P<0.05）。尿IL-18评估AASV病情活动性的曲线下面积（AUC）为0.816（P<0.05）,当检测的截点值为0.130 pg·mL-1·Cr-1时,其敏感度和特异度分别为85.7%和71.4%。结论AASV肾损害患者尿液IL-18水平升高,与疾病活动性相关,在早期预测AASV病情及活动性上有一定价值。福建省自然科学基金(No.2014J01430

Distribution and Species of Mercury in Water and Sediments from Huangpu River

黄浦江江水的总汞、溶解态汞和颗粒汞含量变化较大,其平均值分别为(0.4±0.44)ng/mL、(0.27±0.42)ng/mL和(0.13±0.10)ng/mL,江水中汞以溶解态汞为主.黄浦江沉积物的总汞含量为70.52ng/g~387.30ng/g,平均汞含量为(204.03±97.41)ng/g.江水和沉积物中汞的沿江分布具有中游高,上游和下游低的特征,西渡—南浦大桥江段汞含量为整个黄浦江最高的江段,汞的分布特征与两岸工农业布局相一致.沉积物总汞与有机质显著相关,沉积物中高汞含量的地点都在高水汞点的下游,与河流的动力沉积特点一致.沉积物中汞以可交换态、腐殖酸结合态、残渣态为主,少量为碳酸盐结合态.从上游到下游,沉积物中可交换态汞具有两端高中间低的特点,而残渣态汞与此相反.在剖面方向上,沉积物中的汞主要集中在残渣态,少量为腐殖酸结合态,可交换态及碳酸盐结合态,随着深度增加残渣态所占比例不规则增加.愈接近长江口,沉积物中的重金属愈容易被重新激活.Levels of total mercury, soluble mercury and particle mercury in water of Huangpu River change greatly, their average values are (0.40.44)ng/mL, (0.270.42)ng/mL, (0.130.10)ng/mL respectively. Mercury in water is mainly in the form of soluble mercury. Average mercury content in sediment of Huangpu River is relative high and up to (204.0397.41)ng/g, with a range of 70.52ng/g387.30ng/g. Mercury content is high in the middle reach of Huangpu River, especially in section of Xidu-Nanpu Bridge, and low in upstream and downstream. Distribution of mercury is hightly related with distribution of industry plants and farming. Locations with high mercury content in sediment are in the downstream of locations with high mercury content in water. Mercury (in sediments) is mainly in the form of residue, exchangeable ions, and humics-bound, seldom is in the form of carbonate-bound. Contrary to residue-bound mercury, exchangeable mercury is low in the middle reach, and high in upstream and downstream. There mainly are residue-bound mercury and little humics-bound mercury, exchangeable mercury, and carbonate-bound mercury in sediment in profile, and the residue-bound mercury increases irregularly with depth. Nearing the Mouth of Yangtze River, mercury in sediment becomes more active.国家自然科学基金资助项目(20077016);; 中国博士后基金项目(2002032148);; 厦门大学行动计划联合资助项目(0660-X01114

The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors

本文采用一步法制备了1-芘丁酸/石墨烯复合物(PBA/G)，研究了其电化学性质. 采用铁氰化钾和亚铁氰化钾电化学探针测定了电化学阻抗滴定曲线，确定了PBA/G的表观pKa为6.2. 此外，将葡萄糖氧化酶(GOD)共价键合在PBA/G表面构建了葡萄糖电化学传感器，其电化学响应与葡萄糖浓度（5 mmol L-1浓度范围内）呈线性，检测限为0.085 mmol L-1. 实验还测定了固定在PBA/G表面的GOD的表观米氏常数为5.40 mmol L-1，表明固定化的GOD对葡萄糖有较高的催化活性。The electrochemical properties of 1-pyrenebutyric acid/graphene composites (PBA/G) obtained by one-step synthesis via &pi;-&pi; stacking was investigated. The electrochemical impedance titration curve shows the surface charge changes as function of solution pH by using ferricyanide/ferrocyanide redox couple as the probe. An apparent pKa value is estimated as 6.2 according to the impedance titration curve. In addition, a glucose biosensor was constructed by immobilizing glucose oxidase (GOD) on the surface of PBA/G via covalent interaction. This biosensor shows a linear response to glucose within the concentration up to 5 mmol L-1 with a detection limit of 0.085 mmol L-1. A small apparent Michaelis-Menten constant (5.40 mmol L-1) of the immobilized GOD suggests that the immobilized GOD retains its bioactivity and shows high catalytic activity to glucose.This work was financially supported by the National 973 Basic Research Program (No. 2012CB933804), the National Natural Science Foundation of China(No. 21035002, No. 21121091) and the Natural Science Foundation of Jiangsu province (BK2010009)This work was financially supported by the National 973 Basic Research Program (No. 2012CB933804), the National Natural Science Foundation of China(No. 21035002, No. 21121091) and the Natural Science Foundation of Jiangsu province (BK2010009)作者联系地址：南京大学 化学化工学院，生命分析化学国家重点实验室，江苏 南京 210093Author's Address: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China通讯作者E-mail：[email protected]