Expression and clinical significance of RRM1,CDX2 in gastric cancer tissues

目的探讨RRM1、CDX2在胃癌组织中的表达,并分析其与胃癌侵袭与转移的关系。方法选取90例术前未行放化疗的胃癌石蜡标本及与其对应正常胃黏膜蜡标本30例,采用SP免疫组化方法检测RRM1和CDX2在两种组织中表达情况,分析二者表达与分化程度、浸润深度、淋巴结转移、TNM分期之间的关系。结果 （1）RRM1、CDX2在胃癌中的阳性表达率均显著高于正常胃黏膜组（86.7%vs 40.0%,P〈0.01;61.1%vs 16.7%,P〈0.01）。（2）90例胃癌中,RRM1与CDX2二者之间呈正相关关系（r_s=0.223,P=0.034）;RRM1、CDX2表达与分化程度密切相关（P〈0.05）,CDX2表达与浸润深度、淋巴结转移、TNM分期密切相关（P〈0.05）,RRM1表达与浸润深度、淋巴结转移、TNM分期无相关（P〉0.05）。结论 RRM1、CDX2可作为较为理想的肿瘤标志物,用于胃癌的诊断和预后的预警。Objective To explore the expression of RRM1 and CDX2 in gastric cancer tissues and its relationship with invasion and metastasis of gastric cancer. Methods Expression of RRM1 and CDX2 was detected in 90 cases of gastric cancer without preoperative chemoradiotherapy and 30 cases of normal gastric mucosa using immunohistochemistry SP method. The relationships of RRM1 and CDX2 expression with differentiation degree,tumor invasion depth,TNM stage and lymph node metastasis were analyzed. Results（1）The positive expression rates of RRM1 and CDX2 were both significantly higher in gastric cancer tissues than in those of normal gastric mucosa（86. 7% vs 40. 0%,P 〈 0. 01; 61. 1% vs 16. 7%,P 〈 0. 01）.（2） The expression of RRM1 in 90 cases of gastric cancer was positively correlated with CDX2（ r_s= 0. 223,P = 0. 034）. The expression of RRM1 and CDX2 was related with differentiation degree（ P〈 0. 05）. The expression of CDX2 was related with tumor invasion depth,TNM stage and lymph node metastasis（ P 〈 0. 05）. The expression of RRM1 was not related with tumor invasion depth,TNM stage,lymph node metastasis（ P 〉 0. 05）. Conclusion RRM1 and CDX2 can be used as ideal tumor markers in diagnosis of gastric cancer and prognosis.福建省教育厅B类科技基金资助项目（JB12319

Self-Cleaning Glass Antireflection Films Prepared by Sol-Gel Method

在酸催化体系中以正硅酸乙酯(TEOS)和纳米锐钛矿型二氧化钛粉末为SIO2-TIO2复合溶胶原料,采用提拉法在高硼硅玻璃基片表面制备出具有增透和自清洁功能的薄膜。研究了制备工艺对薄膜增透和光致超亲水性能的影响。制备的样品在400~800 nM波段,光线垂直入射时,透射率增加3%,紫外光照射一定时间后,样品对水接触角不超过5°,具有光致超亲水作用。The self-cleaning glass antireflection film was prepared on the surface of high borosilicate glass substrate by using TEOS and nanometer anatase type of titanium dioxide powder as sol-gel SiO2-TiO2 composite raw materials and using Czochralski method.The effect of preparing technique on antireflection and photoinduced super hydrophilicity properties was investigated.The experiments show that the light transmittance of prepared sample increased about 3% at wavelength between 400nm and 800nm under light vertical incidence.After UV irradiation for a certain time,the water contact angle was ≤5° which exhibited the characteristic of photoinduced super hydrophilicity.福建省区域科技重大项目(2011H41010083

In situ probing electrified interfacial water structures at atomically flat surfaces using Raman spectroscopy

自然界中水无处不在，人们对水分子的研究已经长达一个多世纪。特别是在材料表面，从原子结构层面理解界面水所发生的各种物理和化学过程，将有利于指导能源和环境领域中开发更好的技术和器件。文献中采用振动光谱已经推知了水在金属界面的不同构型，如四配位水、三配位水、表面特性吸附水、自由水等。然而，仍然缺乏这些界面水在不同电位下清晰的构型图像。李剑锋教授课题组采用不受体相水干扰的表面增强拉曼光谱，首次在金（111）和（100）单晶电极表面上获得了界面水的拉曼信号，并且在析氢反应过程中原位观测到了界面水的两种构型转变。发现界面水随着电位的负移，由“平行”结构向“单端氢朝下”，再向“双端氢朝下”变化。程俊教授课题组采用从头算分子动力学理论方法，模拟出不同电位下，在双电层中界面水的三种构型以及相应的氢键数目，与实验数据很好地吻合，进一步揭示了双电层的原子级结构。该研究首次在实验和理论层面，将界面水的构型转变以及氢键断裂与精确的电极电势标尺进行关联，对探知双电层的三维结构具有指导性意义。 该研究工作通过厦门大学校内合作完成，化学化工学院李剑锋教授和程俊教授为通讯作者，田中群教授提供了重要指导。李超禹博士（现美国麻省理工学院博士后）和乐家波博士（现能源材料化学协同创新中心博士后）为共同第一作者，王耀辉博士生在实验上提供了帮助，物理系的陈舒博士和杨志林教授为本工作开展了电磁场增强计算【Abstract】Molecular structures of solid/liquid interfaces are of fundamental interest, and play significantly in the efficiencies of energy storage and conversion. To elucidate the structures of electric double layers at electrochemical interfaces under bias potentials, we have collaborated in situ Raman spectroscopy and ab initio molecular dynamics, and for the first time distinguished two structural transitions of interfacial water at electrified Au(111) and Au(100) single crystal electrode surfaces. Towards negative potentials, the interfacial water molecules evolve from structurally “parallel” to “one-H-down”, and further to “two-H-down”.Concurrently, the number of hydrogen bonds among the interfacial water shows an overall decrease along with the negative shift of the potential, and undergoes two transitions as well. Our findings shed light on fundamental understanding of electric double layers and electrochemical processes at the interfaces.National Natural Science Foundation of China (Grants No. 21373166, 21522508, 21775127, 21521004, 21321062 and 21621091). 该工作得到国家自然科学基金委的大力资助，也得到了固体表面物理化学国家重点实验室、谱学分析与仪器教育部重点实验室、能源材料化学协同创新中心的支持

Controller of central air-conditioning fan coil

本实用新型涉及中央空调风机盘管控制技术,具体说是一种基于无线通信的中央空调风机盘管控制器。包括控制模块、传感器模块、按键模块、液晶显示模块和控制输出模块五部分;其中,控制模块存有控制程序,用于运行控制策略,通过网络控制器与上位机通信;传感器模块采集环境温度信息;按键模块接受用户的按键操作指令;液晶显示模块显示中央空调风机盘管控制器的运行状态、中央空调风机盘管控制器的运行模式和环境温度;控制输出模块用于控制中央空调风机盘管的运行。本实用新型适用于大型建筑的节能改造,可根据环境温度实现对中央空调风机盘管的调节,降低空调系统运行能耗,同时由于采用了无线通信技术,可节省大量布线,降低系统造价

基于无线通信的阀门泄漏检测系统

阀门泄漏的在线监测一直是困扰流程工业的难题,采用传统有线监控方式需要巨大的布线安装成本。本文通过结合无线通信技术和超声检测技术,研究并开发了一种基于无线通信的阀门泄漏检测系统,可极大地降低测控系统的投资和使用成本,通过实时检测阀门的工作状态,及时发现并定位泄漏位置,实现工业生产的节能降耗

Wireless network based wireless transmission equipment

本发明涉及无线网络集中抄表系统，具体说是一种基于无线网络的无线透传设备。本发明安装于电表集中器中，包括无线透传模块、指示灯、电源处理模块、电平转换模块和电源输出模块；其中，无线透传模块经电平转换模块与电表集中器中的中央处理器进行串行通信，无线透传模块通过无线网络与用户终端电表进行通信，无线透传模块的工作电源端通过电源处理模块接收外供电源，电源处理模块的输出端还通过电源输出模块与电表集中器中的光耦相连，无线透传模块的输出端接有指示灯。本发明采用无线的方式进行定期集中抄表，即提高了工作效率，又减少了人为的误抄，还大大节约了成本，并且可以实现自动化控制

Cloning and Polymorphism of MHC Class IA Gene from Epinephelus akaara

采用rACE技术,成功克隆获得赤点石斑鱼(EPInEPHEluS AkAArA)非特异性免疫因子MHC IA基因的4个CdnA全序列,序列全长为1 680bP,含有3′uTr、启动子、多肽结合区(α1)、IgC区(α2)、跨膜区、胞质区和5′uTr区,编码区大小为1 074~1 080bP,共编码357~359个氨基酸,推测蛋白质分子质量约41.66ku.氨基酸序列分析发现赤点石斑鱼MHC IA分子具有经典MHC蛋白分子的空间结构,在多肽结合区则存在极其丰富的变异.利用rEAl TIME PCr和SSCP技术研究了赤点石斑鱼MHC IA的组织表达特异性和表达多态性,发现其在头肾、心、肝等12个组织器官中均能有效表达,在头肾,脾,胸腺等免疫组织表达量不仅高,表达多态性也异常丰富,而在与外界环境接触较多的鳃、肌肉、肠等组织表达较弱.此外,根据MHC IIb分子中相对保守的IgC区氨基酸序列构建了脊椎动物的系统进化树,也表明了该分子的IgC区氨基酸序列可以作为研究鱼类物种间进化关系的良好标记.The major histocompatibility complex molecules(MHC I and MHC II) are fundamental components of imumune response to foreign protein antigens,and occur extensively in all vertebrate organisms.For the first time,4 complete cDNA sequences of MHC class IA chain,one of the important non-specific immune genes,were obtained from Epinephelus akaara by RACE technique.The whole sequences had 1 680 bp,which consist of:a 3′UTR,promoters,a peptide-binding region(α1),an immunoglobulin-like region(α2),a transmembrane region,a cytoplasmic region and 5′UTR,and the size of their coding region is 1 074-1 080 bp,which encode a 155 amino acid protein and has a molecular weight about 30.25 ku.The tested gene has classic MHC 3D molecular structure,and exhibits abundant polymorphisms in the peptide-binding region.Using realtime PCR and SSCP technique,the MHC IA gene was observed to be expressed in all the 12 tissues studied in this paper,and high levels of expression and polymorphism were observed in immune organs such as head-kidney,spleen and thymus,while in organs such as gills and muscles that are more likely to be frequently exposed to bacterial antigens,the expression and polymorphism levels were lower.Furthermore,a phylogenetic tree was also constructed based on Neighbour-joining methods with IGC region of MHC IIB gene.The satisfactory result also meaned that the amino acid sequence of the IGC region of MHC IA should be potentially informative for phylogenetic studies in fishes.福建省种业创新与产业化工程项目(闽种石斑004号

Uncovered canal flux measurement system based on wireless sensor networks

设计了一种将无线传感器网络和超声波测距技术相结合的巴歇尔槽明渠流量测量系统,该系统中将超声波测距元件与无线传感器节点相连,来测量液位的高度值,进而换算得到该流量值,无线传感器网络节点遵从ZigBee无线通信协议进行通信,并在人机界面显示各流量值

水合物分解渗流导致出砂测量的实验方法及实验装置

本发明提供一种水合物分解渗流导致出砂测量的实验方法及实验装置，包括以下步骤：向样品管中填充实验材料，模拟水合物沉积层；将所述样品管放置在8～30MPa、0～10℃的环境中；向所述样品管中充甲烷气体和水，以模拟水合物分解和开采；通过滤网模拟水合物分解渗流，所述样品管中的水砂气混合物经滤网后甲烷气体、水与部分砂混合物流出；甲烷气体向上流经流量计并获得体积，水砂混合物向下流经核磁共振测量模块并获得砂的体积。本发明可以实时测量水合物分解渗流过程中温度与压力、出砂量、出气量和出水量，为水合物开采出砂预测和防治措施制定提供科学依据和技术支持