Functions of DHP and its nuclear receptor on reproductive neuroendocrine in zebrafish (Danio rerio)

本文研究了DHP对野生型和孕酮核受体敲除纯合型两种类型雄性斑马鱼垂体中促性腺激素β亚基基因（fshb和lhb）、脑中多巴胺合成限速酶酪氨酸羟基化酶基因（th2）及多巴胺二型受体基因（drd2a和drd2b）和促性腺激素释放激素基因（gnrh2和gnrh3）mRNA表达的影响。主要研究结果如下： 1.野生型雄性斑马鱼垂体中促性腺激素β亚基基因（fshb和lhb）mRNA表达呈现周日变化趋势，可分为基础表达期、上升表达期和高峰表达期3个期，高峰表达期的mRNA表达量显著高于其它2个期。野生型雄性斑马鱼活体暴露于DHP水溶液后，垂体中fshb和lhbmRNA表达量随DHP暴露浓度的增加和暴露时间的...We examined the effects of DHP on the fshb, lhb, th2, drd2a, drd2b, gnrh2 and gnrh3 mRNA expression in WT and pgr-/-. The main results were as follows: 1. fshb and lhb mRNA expression of WT showed diurnal change. The level of peak expression phase was significantly higher than those of other two expression phases. After WT were exposed to DHP in vivo, fshb and lhb mRNA expression showed a upward ...学位：理学博士院系专业：海洋与地球学院_海洋生物学学号：2232012015345

Studies on the regulation of membrane microdomains (lipid rafts) / Flot to mitogen-activated protein kinase signaling pathway and its effect on reversing multidrug resistance of hepatocellular carcinoma cells

目的： 1.构建耐药肝癌细胞模型。 2.检测Flot下调对ERK1/2信号通路的影响，明确能否通过Flot调控ERK1/2信号通路从而实现肝癌细胞耐药性的逆转。 3.检测脂筏破坏剂甲基-β-环糊精破坏脂筏结构对ERK1/2的影响，探索脂筏与ERK1/2之间的关联。 4.检测下调Flot对耐药肝癌细胞侵袭能力的影响。 5.构建过表达质粒Flot1/pcDNA3.1（+）、Flot2/pcDNA3.1（+），为后续研究工作奠定基础。 方法： 1.用阿霉素大剂量冲击法诱导细胞，Celltiter-Glo荧光细胞活性检测法检测细胞对阿霉素的敏感性。 2.设计siRNA和shRNA，Re...Objective： 1. To induce poly multidrug resistant (MDR) cell models from human hepatocellular carcinoma (HCC) cell lines by chemotherapy drugs and detect resistance of the developed HCC MDR cells. 2. To detect the effects of ERK1/2 pathway by down-regulating Flot on HCC drug-resistant cells, thus to explore if down-regulating Flot could affect ERK1/2 pathway and reverse resistance of HCC MDR cell...学位：医学硕士院系专业：医学院_病理学与病理生理学学号：2452012115310

Network Business Marketing Strategy Research of HXB

摘要 随着现代信息化技术的高速发展，民众的传统生活方式发生了重大转变，进而对于传统行业的营销手段以及方式也提出了新的要求以及挑战。就我国商业银行为例，在信息化高速发展的今天，通过网络业务的方式开设，我国商业银行能够有效的降低在客户服务过程中的运营成本，如何有效的通过网络业务的开展，强化商业银行市场竞争力以及服务质量、效率的提升成为了制约当代商业银行网络业务发展的关键。 网络业务营销是今后商业银行的重要营销方式，同时也是今后商业银行竞争的重要战略市场体现，在优化自身网络业务营销策略推行的过程中，商业银行必须优化以网络业务营销基础平台为依托，同时结合现代客户的需求转变点为基础，通过针对化的...Abstract With the rapid development of modern information technology, people's traditional way of life has changed greatly, and it also puts forward new requirements and challenges for the traditional industry marketing tools and methods. As an example, in the development of information technology, the commercial banks can effectively reduce the operating costs in the process of customer service....学位：工商管理硕士院系专业：管理学院_工商管理硕士(高级管理人员工商管理硕士)学号：X201315619

Exploration on Social Assistance to the Poor Elderly in Fuzhou

社会救助作为伴随社会发展而出现的一项社会保障制度，对于缓解老年贫困，提高老年人的福祉具有重要意义。贫困老年群体因其收入低、健康状况差而被低保、医疗救助、特殊困难群体救助等多项救助制度覆盖。在我国社会经济发展进步的过程中，老年社会救助制度也逐渐系统化、科学化。然而我国老年社会救助还存在救助水平不高，救助内容偏少，救助覆盖范围比较窄等问题。 不论是对于建立和完善具有中国特色的老年社会救助制度，还是为经济发展提供一个稳定的社会环境，对贫困老年社会救助这项制度的研究都具有重要意义。本文以福州为个案，通过实证分析，试图对福州市“十二五”期间贫困老年社会救助情况进行梳理与深入分析，在此基础上提出完善贫困...Social assistance,as one of the social security system accompanied with the development of society, is of great significance to alleviate poverty and improve the welfare of the elderly. The poor elderly population, due to their low income and poor health,is well protected by the subsistence allowance, medical assistance and special relief and other relief system. With the improvement of China's so...学位：公共管理硕士院系专业：公共事务学院_公共管理硕士学号：1392011115043

Study on Embryotoxicity and Bioaccumulation of the Combined Exposure of Typical Antibiotics in Oryzias Melastigma Embryos

抗生素以其优良的抗菌作用，被人类广泛地使用，致使其以各种途径，大量地进入到自然环境中，尤其是水环境中。其中，磺胺类和氟喹诺酮类药物因其广谱的抗菌性而被大量使用，因此成为水环境中检出率最高、检出浓度最大的两类抗生素。目前，环境中抗生素的研究主要集中于痕量检测，对于水生生物毒性研究多针对于大型溞、浮萍、月芽藻等低等水生生物，而对低剂量、长期暴露下抗生素对鱼类的生理毒性效应，及其在生物体内的富集研究相对较少，其在水环境中潜在的生态毒理效应尚未有明确的评价，有必要对相关的生态毒理数据进行完善，同时，研究手段上偏重生物学方法，与化学相结合来研究毒性作用机制非常欠缺。 本研究从磺胺类和氟喹诺酮两大类抗生...Antibiotics have been widely used by people with its excellent antibacterial effect, which result in a large number of antibiotics into the environment through a variety of ways, especially water environment. The sulfonamides and fluoroquinolones were widely used, and this has caused that they became one of the highest detection rate and quantity antibiotics. Currently, most reseach focus on trace...学位：理学硕士院系专业：海洋与地球学院_海洋化学学号：2232012115130

Study on the formation mechanism of gelation of konjac glucomannan

利用直流电，在添加CaCl2、MgCl2、FeCl3、钨酸钠的条件下，分别制备了KGM-Ca、KGM-Mg、KGM-Fe、KGM-T凝胶，对获得凝胶后电处理KGM溶胶电极两侧pH进行了测试,并对KGM-T凝胶进行了FTIR、Raman、SEM、XRD、DSC表征。结果表明，正极附近KGM溶胶为酸性，负极一侧KGM溶胶为碱性。SEM表明KGM在粉末状态下，外表呈现规则的微纤维状，KGM分子间有氢键；KGM溶胶冻干后呈现大小孔洞不均匀的支架结构，直流电处理的KGM溶胶冻干后的扫描电镜没有发生明显的变化，电处理未使KGM链的糖苷键发生断裂；KGM-Ca凝胶的表面形貌呈现大小孔洞不规则的絮状结构，KG...Konjac glucomannan (KGM) -Ca 、KGM-Mg 、KGM-Fe 、KGM-tungsten (KGM-T) gels were successfully produced under DC electric fields in the presence ofCaCl2、MgCl2、FeCl3 and sodium tungstate. pH experiments were conducted tomeasure pH value of DC treated KGM sol. And KGM-T gels were characterized byFTIR、Raman、SEM、XRD and DSC. pH experiments showed that KGM solcontaining Na2WO4.2H2O in the vicinity of the p...学位：博士后院系专业：材料学院_软物质与功能材料学号：201317006

Fission life-time calculation using a complex absorbing potential

A comparison between the semi-classical approximation and the full quantum calculation with a complex absorbing potential is made with a model of the fission of 258Fm. The potential barrier is obtained with the constrained Skyrme HF+BCS theory. The life-time obtained by the two calculations agree with each other the difference being only by 25%.Comment: 5 pages, 2 figures, Conference proceedings of CNR*15 workshop, Tokyo, October 2015 to be published in EPJ Web of Conference

An Empirical Study of Cross-industry Brand Alliances

伴随着扁平化世界的到来，跨行业品牌联合为企业带来了新鲜活力，越来越多的企业投身到该联合中来。部分学者采用实验法对虚拟品牌或者真实品牌的虚拟联合进行了探究，鲜有将跨行业品牌联合作为一个专门的研究课题。为了展现当前跨行业品牌联合的实际状况以及投资者对企业联合声明的态度，本文以国内外实际采用了跨行业联合行为的130对企业共计286家企业为样本，对这些企业参与跨行业品牌联合的基本形态进行了分析。结果发现：从联合目的而言68.2%的企业参与跨行业品牌联合是为了获得资金与销售上的优势，只有91家企业是为了获得声誉或者形象上的提高。形式上有54.6%对企业采用的是促销形式的联合，其次是在产品与渠道上的合作，...With the arrival of the flat world, more and more enterprises tend to enter different types of brand alliances with varying motives. Some scholars used real or hypothetical brand in hypothetical alliances with experimental method, however, little work has been done on the exsiting cross- industry brand alliances and the attitudes about stockholders to the alliance announcements. In this paper, we...学位：文学硕士院系专业：新闻传播学院广告学系_传播学学号：3192008115308