Identification of Candidate Plasma Altered miRNA Associated with Bone Metastasis of Lung Cancer

目的：应用高通量miRNA芯片及荧光定量PCR技术筛选及鉴定肺癌骨转移血浆中差异表达的miRNA。 方法：搜集2013至2015年在厦门大学附属第一医院入院的45例肺癌病人外周血标本。选取4例肺癌骨转移与4例肺癌无骨转移病人血浆，应用miRNA芯片技术对差异表达的miRNA进行筛选。应用IPA分析差异miRNA参入的信号通路。应用Real-timePCR检测候选差异表达miRNA，进行验证。 结果：通过比对，成功建立了肺癌骨转移与肺癌无骨转移血浆miRNA芯片表达谱，筛选出了12个差异表达miRNA，其中在肺癌骨转移患者血浆中表达上调的miRNA是5个，下调的是7个。选定miRNA-874...Objective: The main aim of this study is to compare expression profile of miRNAs between lung cancer patients with bone metastasis and lung cancer patients without bone metastasis and to identify different expressed miRNA associated with bone metastasis by using miRNA microarray technology and quantitative real time PCR. Methods: Blood samples were obtained from 48 lung cancer patients including ...学位：医学硕士院系专业：医学院_肿瘤学学号：2452013115347

The Study of Firm In the Bionic View

本文综合运用管理学、经济学、生物学、统计学等理论与方法，对企业仿生进行了系统的研究。在介绍企业仿生相关理论、构建企业生命体假设、研究企业进化与环境关系、引入企业基因重组模型后，特别以IBM公司为例，运用DEA模型对其企业效率进行实证研究。具体研究内容及研究成果包括： 第一章，全面介绍了本文的研究背景、理论基础以及研究的方法和内容。 第二章，通过汲取企业遗传理论中的有关研究理论和方法，构筑企业基因的基本概念框架。在参阅了大量文献的基础上重新定义企业基因、企业DNA的概念，构建了企业基因概念体系。 第三章，通过对企业外部环境的概念的界定，揭示了企业外部环境对企业进化的影响，探析了企业对其外部...In this dissertation,a systematic research has been conducted on the enterprise bionics,employing the knowledge and methodology of the management science,economics,biology and statistic comprehensively. Some important theories and concepts,such as enterprise bionics theory,enterprise gene theory,the relation of the business evolution and environment,as well the mechanism of Enterprise Gene Recombi...学位：经济学硕士院系专业：经济学院计划统计系_国民经济学学号：1542009115166

我国货币市场和资本市场连通程度的动态分析

本文通过实证研究发现,我国货币市场与资本市场之间不存在紧密的联系。通过动态分析,我们认为一个市场上的资金变动和价格变动无法影响另一个市场上的资金变动和价格变动,虽然这些价格和资金分隔的情况在逐渐消除。我们还发现交易所债券市场的即期利率初步具备了合理的期限结构图形,而银行间债券市场还没有。本文最后为两个市场的连通提出政策建议。Dynamic ananlysis shows no evidence of close interconnection between China's money market and capital market .The changes of trading volume and price in one market can not influence those of the other market,though the segmentation decreases over time.We also find that the term structure of interest rate in bond market in Shanghai Stock Exchange is more effective than in the inter-bank market.Finally we offers some policy suggestions as for the interconnection between these two markets.国家自然科学基金资助项目(70403011)的资助;; 中国博士后科学基金项目(2004035292

Promotion of Agricultural Management through Member Organization Activities of Agricultural Cooperatives ーThe Case of JA Osaka Nakakawachi and Kashiwabara House Grape Shippers Associationー

This paper focuses on the Kashiwabara House Grape Shippers Association, a member organization of JA Osaka Nakakawachi. First, we would like to analyze what kind of activities the member organization is performing to demonstrate its organizational strength in the agricultural cooperative organization, and clarify its functions and issues, as well as the direction of its future development. Specifically, what role do member organizations play in bringing out the organizational strength of agricultural cooperatives? Alternatively, we would like to examine what conditions are necessary to strengthen organizational strength. In this paper, we will survey past research on the organizational strength of agricultural cooperatives and, based on the results of field interviews, examine the role of member organizations and their role in bringing out the organizational strength of agricultural cooperatives. JA Osaka Nakakawachi is blessed with natural conditions suitable for agricultural production, but has been strongly affected by the urbanization of Osaka Prefecture. As a result, agricultural management in JA Osaka Nakakawachi has developed in the form of urban agriculture, and the size of farmland and management is quite limited compared to the national average. The main agricultural products are vegetables and fruit trees such as grapesdepartmental bulletin pape

The Development of and Challenges for Agricultural Cooperatives in Japan

departmental bulletin pape

新規就農者支援における農業協同組合の役割 : JA大阪中河内, JA松本ハイランドの事例を中心に

Currently, Japan is facing a chronic shortage of agricultural labor due tothe serious problem of young farmers leaving farming. Needless to say,securing new farmers has a significant impact on the maintenance anddevelopment of the agricultural sector.Under these circumstances, securing new farmers is attracting attentionas one of the solutions to Japan’s agricultural successor problem. Supportpolicies for new farmers are considered to be a major key to increasing thenumber of new farmers and improving the retention rate. The role ofagricultural cooperatives is thought to have a certain impact on securing acertain number of new farmers and improving the retention rate.In this paper, we use Matsumoto Highland Agricultural Cooperative inNagano Prefecture and Osaka Nakakawachi Agricultural Cooperative inOsaka Prefecture as case studies to analyze the policies of eachagricultural cooperative with regard to support for new farmers and toclarify the support system and challenges faced by agriculturalcooperatives in securing new farmers

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

Nitrogen transports and transformations in the Wuchuan headwater stream

利用氮稳定同位素法、综合常规水样监测以及微环境培养方法探讨了五川农业溪流沿程氮的迁移转化过程。结果表明,溪流沿程nO3-n浓度变化范围为0.5--4.5 Mg l-1,平均为1.26 Mg l-1,且多数月份总体上呈逐渐升高的趋势,同时nO-3的δ15n存在降低趋势,两者呈线性负相关(r2>0.80),表现明显的硝化过程典型特征。各采样点的nO2-n、dO、dOC浓度与nO3-n也存在一定的线性相关关系,说明了硝化过程的发生和氮在不同形态之间的转化。溪流水体微环境培养实验证明了水体中的nH4-n和nO3-n浓度在培养期内并无明显变化,以上数据和相关关系充分表明了流域沿程地表径流氮素输入和溪流系统沉积物和水体界面的硝化过程共同作用可能是五川溪流沿程nO3-n与nH4-n浓度升高,而nO-3的δ15n值减小的主要原因。因此,在农业流域的污染源评价中,需要重新审视和调查河道系统内部氮的转化和贡献。Nitrogen isotope and traditional water sampling,together with microcosm incubations were used to investigate processes of nitrogen downstream transports and transformations within the Wuchuan stream.Results showed NO3-N concentrations varied downstream ranging from 0.5 to 4.5 mg L-1 for most of sampling months with a mean of 1.26 mg L-1 in the stream.However,nitrate isotope δ15N decreased downstream,comparing to increased trend in NO3-N concentration.The relationship between downstream NO3-N concentrations and nitrate δ15N with a R2 exceeding 0.8 can be characterized as a typical process of nitrification.The relationships between NO3-N concentrations and NO2-N,dissolved oxygen,dissolved organic carbon concentrations for the sampling sites also indicated strong nitrification processes and consequent nitrogen transformations between nitrogen forms in the stream.Microcosm study of surface water from the stream showed that no significant change in NH4-N and NO3-N concentrations occurred during the five-day incubations.All the information and relationships showed that the downstream increases in NH4-N and NO3-N concentrations, and the downstream decreases in nitrate δ15N within the stream obviously resulted from the nitrogen input from surface runoff flowing into the channel,and also nitrogen transformations by nitrification occurred between stream sediment and overlying water within the channel.Therefore,it is essential to take the sources from nitrogen transformations within streams into account and to evaluate their contributions in pollution assessments at catchment scales.国家自然科学基金资助项目(40671116);福建省自然科学基金重点资助项目(D0720002);国家教育部新世纪优秀人才支持计划资助项目;福建省高校新世纪优秀人才支持计划资助项目;厦门市科技计划指导性资助项目(3502Z20077014

Application Assessment of Psychological Counseling through Hotline during the SARS Epidemic Period

目的　探讨热线电话心理咨询在SARS流行期间的作用 ,评价突发公共卫生事件时电话心理咨询者的一般情况及咨询问题类型。方法　对 5月份SARS流行期间来自全国的 366人次的心理咨询记录进行分析 ,比较不同地区、性别、年龄和咨询内容的差异。结果　在来自全国的热线电话咨询者中 ,以北京为多 ,女性为多众 ,青壮年为主体 ,情绪问题较为突出。结论　在疫情较重的时期和地区 ,情绪问题占首位 ,心理咨询热线电话是公众寻求心理支持的有效途径

中国海南黎族人群15 个STR 位点的遗传多态性

【目的】研究海南黎族族人群15个STR位点D3S1358、TH01、D21S11、D18S51、VWA、CSF1PO、D8S1179、TPOX、FGA、D5S818、D13S317、D7S820、D16S539、D19S433、D2S1338的遗传多态性。【方法】通过人类短串联重复序列(short tandem repeat，STR)复合扩增、基因扫描、基因分型调查了110名黎族无关个体15个STR位点等位基因分布情况。【结果】15个STR位点均符合Hardy-Weinberg平衡，共检出149个STR等位基因，其频率分布在0.0045～0.5045之间，杂合度(heterozygosity，H)为0.6716～0.8754，个体识别力(discrimination power，DP)为0.8770～0.9673，非父排除率(probabilities of paternity exclusion，EPP)为0.4383～0.7396，多态信息含量(polymorphic information content，PIC)为0.6265～0.8574。【结论】选用的15个STR位点均能检出等位基因遗传多态性，并具有较丰富的信息含量，为进一步研究中华民族STR遗传结构提供了基础资料