红色荧光蛋白在毕赤酵母中的高效表达

报道了红色荧光蛋白(DsRFP)在毕赤酵母中的高水平表达.利用PCR技术从YEpFLAG- 1 -DsRFP扩增出DsRFP编码序列,克隆到毕赤酵母胞内表达载体pPIC3. 5K构建重组表达载体pPIC3. 5K- DsRFP,电击转化进毕赤酵母,G418 RDB平板双重筛选后获得重组转化子,经MM/MD平板培养与PCR鉴定,重组子全部为HIS+MUT+表型.重组菌株诱导表达48h后获得了高效表达,摇瓶中表达水平达到细胞内总蛋白的12%,表达量达到1. 8g/L.经硫酸铵分级沉淀,DEAE- 5PW阴离子交换柱层析与S- HyperD阳离子交换柱层析后获得电泳纯蛋白.说明我们建立的毕赤酵母表达应用平台具有高效表达外源蛋白的能力

System Design and Application of a Miniature Climbing Robot

针对一种小型的双足爬壁机器人,设计开发了基于DSP2812处理芯片的控制系统.该机器人系统采用双足真空吸盘式结构和用3个电机驱动5个关节的欠驱动结构.双足真空吸盘式结构使其可以在光滑的墙面和天棚行走,又能够在交接面之间完成跨步行走.而欠驱动结构减少了电机的数目,从而减小了机器人的尺寸和降低了机器人的质量和能量消耗,但它也给机器人的控制和运动规划带来了新的挑战.已完成的系统设计包括运动模式设计、关节控制、通信模块设计和吸盘足控制等.实验结果证明了所提出方案的可行性

ANALYSIS OF HYDROLOGIC SECTION OFF ZHUJIANG RIVER ESTUARY IN NORTHERN SOUTH CHINA SEA DURING VARIOUS SOUTHWEST MONSOON PHASES

根据2000年7月及2001年5月南海北部珠江口外断面CTD调查资料、同期气象资料,并结合该海域历史资料,对调查断面珠江冲淡水扩展范围、跃层变动情况及上升流特征进行了分析,观察到对应于夏季西南季风的不同阶段,调查断面跃层分布与珠江冲淡水影响范围均发生明显变动,升降流的影响也呈现出不同特征:(1)西南季风较强时,断面陆架区上表层受冲淡水影响明显,海区的层化结构明显加强;(2)西南季风较强时,调查断面出现上升流和下降流。研究结果表明:(1)局地风应力与热通量的变化控制了调查断面跃层或混合层的温度和深度的变化,影响着珠江冲淡水的扩展范围,西南季风较强时珠江冲淡水扩展范围变大,调查断面跃层或混合层强度变大,深度变深;(2)夏季西南季风强时调查断面存在上升流,其形成机制为风产生的离岸水体Ekman输运的补偿效应,底地形的变化虽然也造成较弱的外海次表层水涌升,但可能只是加强了上升的速度或强度;(3)夏季西南季风强时调查断面上存在上升流区与下降流区毗邻的现象,下降流成因可能有二,一为近岸流和陆坡流呈相反方向运动形成弱的反气旋涡,二为“上升与下降因相互水体补充的需要而共生”。Based on the CTD data from two cruises off the Zhujiang River Estuary in the northern South China Sea in July, 2000 and in May, 2001, and combining with the meteorological data in the corresponding period as well as historical observations and modeling results in this area, some features at the observed section ZJ are discussed, including the diluted water from the Zhujiang River, spring layer and mixed layer, upwelling and downwelling. It was observed that these sectional hydrological features varied with different seasons: (1) with the strengthening of the southwest monsoon, the surface layer was influenced more intensively by the diluted water from the Zhujiang River and the stratification structure became more evident at section ZJ; and (2) there existed upwellings and downwellings at the section when the southwest monsoon was strong enough. Some conclusions have been reached as below. (1) Local wind and solar flux dominate the temperature and depth of the spring layer and mixed layer, and affect the extension of the diluted water from the Zhujiang River in a positive way. (2) The southwest monsoon plays the most important role in leading to the appearance of upwellings, which transport water across the shelf through the Ekman effect; the topography of the investigated section may enhance the intensity of the upwellings, but can not result in upwellings when the monsoon is weak. (3) The upwelling and downwelling areas coexist at the section when the southwest monsoon is strong. There may be two explanations on the appearance of downwellings, one is weak anticyclonic eddy resulted from the flows in opposite flowing directions along the slope and along the continent, and the other is that "the upwelling and downwelling coexist in need of the water complement".国家自然科学基金资助项目(49636220

Advances in the M_2 Tide Wave Research in the Taiwan Strait

台湾海峡内各种与潮波相关的现象,可以归因于在地形及岸线的影响下自台湾海峡南北两端进入的潮波的相互作用。近20余年来台湾海峡潮波的研究获得显著进展,然而即使作为台湾海峡主要分潮的M2分潮,其相关研究中也还存在着诸多分歧。从台湾海峡M2分潮潮波分布特征及解释研究出发,并针对以往各种研究中差别较大的M2分潮潮流的分布及变化特征,归纳总结了相关研究的主要分歧:(1)对台湾海峡M2分潮潮波的研究的争议主要集中在2点,包括对自台湾海峡南、北两端进入海峡的潮波的作用范围的研究以及台湾海峡内部潮波系统形成机制的探讨;(2)台湾海峡M2分潮潮位分布研究的主要分歧在于对台湾岛西岸潮时潮差的分布特征的解释;(3)台湾海峡M2分潮潮流研究中最大的差异在于对最大潮流同潮时线分布特征的描述,其实质仍为对潮波作用范围的描述;对应于M2分潮最大潮流同潮时线研究观点的差异,在M2分潮圆流点是否存在、圆流点存在位置及圆流点旋转方向的研究上也存在着较大的差异;对台湾海峡内部椭圆率为零的分隔线位置的讨论,主要分歧在于该椭率零线靠近台湾岛附近及澎湖列岛附近时的分布状况。此外,最强流区的位置判定及极值区潮流流速量值的大小问题上也存在差异。Phenomena relating to the tidal wave in the Taiwan Strait can be attributed to the interaction between the tidal waves coming from the north and south entrances of the Taiwan Strait under the influence of topography and coastline. Although significant progress in the studies on tidal waves in the Taiwan Strait has been made in the past 20 years, there are still many differences in the relevant studies. In this paper, staring from the studies on characterisitcs and interpretation of the M_2 tidal wave distributions in the Taiwan Strait and aiming at the differences in relevant studies on the M_2 tidal current distributions and variations, the main differences in relevant studies are summarized as follows: (1) the main controversies over the M_2 tidal wave in the Taiwan Strait are concentrated on two aspects, one is the action ranges of the tidal waves coming from the two entrances of the Taiwan Strait, and the other is the formation mechanison of tidal wave system in the Taiwan Strait; 2 the main controversies over the M_2 tidal level distributions are the interpretations of the tidal phase and range distribution characteristics along the west coast of Taiwan Island; 3the great differences in the M_2 tidal current studies are the description of cotidal lines of maximum tidal current, including the controversies over the existence, location and rotational direction of the M_2 tidal current amphidromic point and the location of the separation line with ellipticity being equal to zero in the Taiwan Strait. In addition, there are also the controversies over the location of the maximum tidal current area and the magnitude of tidal current speeds in the extremum area.国家自然科学基金资助项目——台湾海峡生源要素生物地球化学研究(49636220;; NFS-C"九五"重点项目);; 中国科学院南海海洋研究所热带海洋环境动力实验室开放课题——台湾海峡生态系统动力学模型研

N-苯基-3-氯-6-(3,5-二甲基-1-吡唑基)吡啶-2-甲酰胺及3-氯-6-(3,5-二甲基-1-吡唑基)吡啶-2-甲酸的合成和表征

以3,6-二氯吡啶-2-甲酸为原料经过酰胺化、肼取代、环合、水解等反应合成了标题化合物,并通过红外光谱、核磁共振谱和质谱对其结构进行了表征。广西民族大学学校科研启动项目(0409032);广西民族大学学校重大项目(0409012);教育部科学技术重点项目(205121);广西民族大学学校重点项目(0509ZD047

Multi-sensor system for inspection robot

本发明涉及一种巡检机器人多传感器系统及巡检方法，包括传感器系统采集巡检机器人当前状态以及外部环境信息，并发送到地面基站；地面基站接收到传感器系统发送的巡检机器人当前状态以及外部环境信息后，向巡检机器人发送控制命令。本发明解决巡检机器人行驶过程中很难获取输电线路全面、准确的环境信息的问题，为巡检机器人越障过程中的自主抓线和动态质心调整提供了保证

一种双臂巡检机器人质心自动调整控制系统和方法

本发明涉及一种双臂巡检机器人质心自动调整控制系统和方法，倾角传感器设置于机器人的控制箱内，且通过A/D转换电路连接处理器，检测机器人的倾斜角度并输出对应的模拟电压信号，通过A/D转换电路转换为数字信号发送给处理器；位移传感器设置于每个关节的运动电机上，且连接处理器，采集各个关节相对于各自零点的位移，发送到处理器；处理器通过RS232通信转换器连接关节电机驱动器，发送控制命令到关节电机驱动器，驱动电机运动。本发明能够在存在建模误差和干扰的情况下，使机器人在双臂交错运动时保持稳定状态

Multi-sensor system for inspection robot

本发明涉及一种巡检机器人多传感器系统及巡检方法，包括传感器系统采集巡检机器人当前状态以及外部环境信息，并发送到地面基站；地面基站接收到传感器系统发送的巡检机器人当前状态以及外部环境信息后，向巡检机器人发送控制命令。本发明解决巡检机器人行驶过程中很难获取输电线路全面、准确的环境信息的问题，为巡检机器人越障过程中的自主抓线和动态质心调整提供了保证