Quantitative analysis of protein-protein interaction in BACTH system using flow cytometry

蛋白-蛋白相互作用是所有生命活动的基础，其研究有助于人们更好地理解蛋白质功能、构建相互作用网络、发现药物靶点、发展疾病诊断和治疗的方法。现有的蛋白-蛋白相互作用研究方法主要分为体外检测和体内检测两种。等温滴定量热法、表面等离子体共振法、荧光偏振法等是常用的体外检测方法，这类方法往往需要使用昂贵的仪器检测以及繁琐的蛋白纯化与标记过程；而酵母双杂交、蛋白质片段重组、合成致死等体内检测方法则只需在细胞和基因水平操作，不仅省去了蛋白质纯化步骤，而且能够真实地反映蛋白质在细胞中的相互作用情况。其中，酵母双杂交系统作为最常用的蛋白-蛋白相互作用研究方法，具有灵敏度高，适用范围广，操作相对简便等优点。其原理...Protein-protein interactions (PPIs) are fundamental to virtually every aspect of life processes. Characterizing the interacting partners of a protein is important in the understanding of protein function, deciphering biochemical networks, discovering novel drug targets, and developing diagnostic and therapeutic assays. Current approaches for PPI study in vitro include isothermal titration calorime...学位：理学博士院系专业：化学化工学院_化学生物学学号：2052011015371

Design and Implementation of Electronic Commerce Platform for Guizhou Alcoholic Commodities

随着酒类销售活动变得越来越多，为提高酒类销售管理的工作效率，减轻商场人员的工作压力，建立一个科学和合理的酒类销售管理系统是非常有必要的。 本文探讨商场酒类销售管理系统的设计和开发，主要从需求分析、系统设计、系统实现和系统测试这三个方面来进行。 1、通过与商场的商品管理人员以及实际操作员工的沟通，获得了贵州酒类电子商务平台的整体业务需求信息，确定系统的总体流程，然后使用UML工具进行系统的业务需求详细分析，获得了系统的功能性需求和非功能性需求。 2、研究基于B/S模式的贵州酒类电子商务平台。系统在开发时采用了自底向上的开发方法，利用了易开发、易部署、高安全性、高效率的J2EE技术。而且在系...As the liquor sales activities become more and more, in order to improve the liquor sales management work efficiency, reduce the store personnel working pressure, the establishment of a scientific and reasonable liquor sales management system is very necessary. This dissertation discussed the design and development of liquor sale management system for market,Mainly from three aspects, which is th...学位：工程硕士院系专业：软件学院软件工程系_工程硕士(软件工程)学号：X201223092

Epidemiological investigation and researches on biology and molecular biology of human hookworms in urban and rural areas of Southern Fujian

钩虫感染仍为当今人体重要的肠道寄生虫感染之一，严重危害人们尤其农民和矿工的身体健康。作者从2005年3月至2009年1月，完成了以下几个部分调查和研究，以期阐明闽南城乡和矿区人体钩虫病的流行病学特征，并对钩虫的生物学及分子生物学进行研究，为设计防治措施提供科学依据。获得了以下主要研究结果： 1．闽南城乡人体肠道寄生虫调查：在厦门、漳州、龙岩和南平等4市调查13个点，共检查了13314人，检出钩虫、蛔虫、鞭虫和蛲虫等肠道寄生虫，总感染率10.31％，感染人数分别为1070、98、33和172，感染率依次为8.04%、0.74%、0.25%、1.29%，构成比分别为77.91％:7.17％:2....To understand infection status and epidemic characteristics and biology of human hookworms in urban and rural and mineral areas of Southern Fujian ,and form control strategy, a study for nearly four years (from March 2005 to January 2009) on human hookworms was carried out , with the help of Professor Cangsang Pan. The main results were as follows: 1. Total 13314 persons were investigated at 13 s...学位：理学博士院系专业：生命科学学院生物学系_动物学学号：2012005140312

A case of drugster infecting Strongyloides stercoralis

本文报道了吸毒者感染粪类圆线虫1例,用肠虫清按常规治疗,治愈。This article reported one case of drugster infecting Strongyloides stercoralis.The patient was cured with albendazole

Discussion about problems concerning design of compaction by blasting in the code

以试验数据为依据,对爆破排淤填石的机理深入分析,进而剖析爆破设计参数的内涵,并对其不足之处进行讨论,通过算例结果指出设计参数的适用情况,供工程技术人员在设计、施工时参考

Transformation of enhanced green fluorescent protein gene in Paecilomyces lilacinus mediated by Agrobacterium tumefaciens

为了实现增强型绿色荧光蛋白基因(EgfP)在生防真菌淡紫拟青霉9410菌株中的转化,借助中间质粒PCdnA3.1(-)构建nPTⅡ-EgfP融合基因的表达载体PuPngT,然后采用根癌农杆菌介导的转化法将EgfP基因转化到淡紫拟青霉9410菌株中。PCr检测和SOuTHErn blOTTIng分析结果表明,EgfP基因以单拷贝形式整合到淡紫拟青霉9410的基因组中。荧光显微镜观察结果显示,转化子在488nM光源的激发下能产生绿色荧光。这些结果说明EgfP基因已成功转化至淡紫拟青霉9410菌株并获得表达。这些工作可为淡紫拟青霉在不同条件下的防效评价、环境安全评价等提供新的途径和方法。The main aim of this study was to transform the enhanced green fluorescent protein gene (egfp) into biocontrol fungus Paecilomyces lilacinus strain 9410.We constructed the expression vector pUPNGT of the fusion gene nptII-egfp using pcDNA3.1(-) as a helper plasmid.The egfp gene was then transformed into P.lilacinus strain 9410 via Agrobacterium tumefaciens-mediated transformation.PCR and Southern blotting analysis showed that the egfp gene was integrated into the genomes of the tested transformants and the integration manner was single-copy.The transformants could generate green fluorescence when they were excited by 488 nm blue laser.These results indicated that the egfp gene had been successfully transformed into P.lilacinus 9410 and expressed in the tested transformants.Our work may provide a new approach to assess environmental safety and practical biocontrol efficacy of P.lilacinus under different conditions.福建省重点项目(No.2008N01050731)资助---

有机光波导放大器最新进展

稀土掺杂平面光波导放大器是一类重要的光子学元件,它是光通信密集波分复用(DWDM)传输系统的重要组成部分,可以和调制器、光开关、隔离器、阵列波导光栅等任何有损耗的器件集成在一起,补偿光传输过程中的各类损耗。综述了应用于第二(1.33μm)、第三(1.53μm)标准通信窗口的有机光波导放大器的研究现状,阐述了不同类型材料制备的器件存在的优缺点、制备过程中存在的问题,并对其发展前景进行了展望

Synthesis of Novel Thiazolo[3,2-a]pyrimidine Compounds

5-乙氧羰基-4-芳基-6-甲基-3,4-二氢嘧啶-2-酮与丁炔二酸二甲酯反应,合成了系列新的噻唑并[3,2-A]嘧啶类化合物,反应具有时间短、收率高、后处理简单等优点.采用nMr(1H,13C,COSy,HSQC和HMbC),Ir等多种谱学技术,结合元素分析对产物进行详细表征,通过对目标产物的1,3-偶极环加成的衍生化产物进行X射线单晶衍射而确定目标产物的结构.A new class of thiazolo[3,2-a]pyrimidine compounds were synthesized by the reaction of 5-carbethoxyl-4-aryl-6-methyl-1,2,3,4-tetrahydropyrimidin-2-ones with dimethyl acetylenedicarboxylate.The reaction has the advantages of excellent yields and simple workup procedures.The products were char-acterized thoroughly by IR and NMR(1H,13C,COSY,HSQC and HMBC) together with elemental analysis.The X-ray crystallographic analysis of the 1,3-dipolar cycloaddition derivative of the title product provided the direct information for the structure of the product.国家自然科学基金(Nos.20772027;20803020);中国博士后科学基金(No.20070410805);卫生部科学研究基金-福建省卫生教育联合攻关计划(No.WKJ2008-2-036);厦门市重大疾病攻关研究基金(No.3502Z20051027);湖南科技大学博士基金(No.E50839)资助项

Theoretical Study on Intramolecular Proton Transfer Reaction in 2-(2-Mercaptophenyl)benzoxazole

在b3lyP/6-31g(d,P)水平上研究了2-(2-巯苯基)苯并噁唑气态中五种异构体(E1,E2,E3,E4和k)在气态中的稳定性及其在基态下的质子转移,同时结合极化连续介质模型(PCM)研究了水、二甲亚砜、乙腈、乙醇、苯胺和环己烷等对2-(2-巯苯基)苯并噁唑溶剂化作用的影响.研究结果表明,醇式异构体E1为2-(2-巯苯基)苯并噁唑的优势构型;在E1向k(酮式异构体)转变过程中,存在一个较小的能垒;当考虑零点振动能(zPVE)后,逆向能垒消失.在溶液中,随着溶剂极性的增强,醇式异构体E1与k之间的反应平衡向k方向移动,在非极性溶剂环己烷中,E1为优势构型,而在强极性水溶液中,k为优势构型。The tautomers(E1,E2,E3,E4,and K) and the ground state intramolecular proton transfer reaction of 2-(2-mercaptophenyl)benzoxazole were studied at the B3LYP/6-31G(d,p) level.The effect of solvent(water,dimethylsulfoxide,acetonitrile,ethanol,aniline,and cyclohexane) was studied at the B3LYP/6-31G(d,p) level,using the polarizable continuum model.The results of density functional calculations indicate that the enol form E1 is the most stable tautomer at the ground state.In these solvents there is an equilibrium for 2-(2-mercaptophenyl)benzoxazole in the ground state between E1 and K,and the equilibrium shifts toward the tautomer K as the polarity of the solvent increases.E1 is the preferential conformation in cyclohexane,but K is the more stable tautomer in water.国家自然科学基金(Nos.20772027;20803020);973子课题(No.2003CB716005);中国博士后科学基金(No.20070410805)资助项

NMR Studies on Interactions between Diperoxovanadate and 1-Ethyl-1H-Imidazole

为探讨过氧钒配合物中有机配体对反应平衡的影响,在模拟生理条件下(0.15MOl·l-1nACl溶液),应用多核(1H、13C和51V)多维(COSy和HSQC)核磁共振(nMr)以及变温技术等谱学方法研究双过氧钒配合物[OV(O2)2l]-(l=d2O或HOd,与之配位的过氧钒物种简写为bPV)和[OV(O2)2ll′]n{-n=1-2,ll′=3-羟基-皮考啉酸根(3-OH-PIC),2-(2′-吡啶)-咪唑(Py-IM),1,10-邻菲啰啉(PHEn),与它们配位的含钒物种分别简写为bPV(3-OH-PIC)、bPV(Py-IM)和bPV(PHEn)}与n-乙基咪唑(n-ET-IM)的相互作用.实验结果表明,n-ET-IM与4种双过氧钒配合物反应性从强到弱的顺序为bPV>bPV(3-OH-PIC)>bPV(Py-IM)>bPV(PHEn).研究表明,金属中心上配体的配位能力、空间位阻和分子量等因素都对反应平衡产生较大的影响,同时竞争配位的结果导致新的6配位过氧物种[OV(O2)2(n-ET-IM)]-的生成.利用上述谱学方法有助于揭示此类相互作用体系的反应过程和配位方式.To understand the effects of organic ligands on the reaction equilibrium, interactions between a series of diperoxovanadate complexes [OV(O2)2L]- (L=D2O or HOD, the corresponding peroxovanadate species (bpV)) and [OV (O2)2LL′]n-{n=1-2; LL′ =3-hydroxyl-picolinate (3-OH-pic), 2-(2′-pyridine)-imidazole (py-im), 1,10-phenanthroline (phen), the corresponding peroxovanadate species bpV(3-OH-pic), bpV(py-im), and bpV(phen)}and 1-ethyl-1H-imidazole (N- Et-im) in solution were explored using multinuclear (1H, 13C, and 51V) magnetic resonance, COSY (correlated spectroscopy), HSQC (heteronuclear single quantum coherence) and variable temperature nuclear magnetic responance (NMR) using 0.15 mol·L-1 NaCl ionic medium to mimic physiological conditions.Experimental results indicated that the reactivity of these four complexes with 1-ethyl-1H-imidazole decreased as follows: bpV>bpV(3-OH-pic)>bpV(py-im)> bpV(phen).The coordinating ability, the steric effect, and the molecular weight of these organic ligands affected the reaction equilibrium.A new six-coordinated peroxovanadate species [OV(O2)2(N-Et-im)]- was formed because of competitive coordination.国家自然科学基金(20772027;20803020);中国博士后科学基金(20070410805);卫生部科学研究基金-福建省卫生教育联合攻关计划(WKJ2008-2-036);厦门市重大疾病攻关研究基金(3502Z20051027)资助项目---