16 research outputs found
Study on the Comprehensive Textbook of Cultural Factors for Teaching Chinese as a Second Language —Taking New Practical Chinese Reader Textbook (5) (6) \ Developing Chinese Advanced Comprehensive Course (Ⅰ)(Ⅱ)as Examples
上世纪60年代起,由于社会语言学、功能语言学、社会心理学等学科的发展,第二语言教学界发生了文化研究的转向,受此影响,上世纪80年代初我国对外汉语教学界也发起了一场关于语言教学和文化教学的大讨论,掀起了一场“文化热”。经过30余年的探索和研究,有关文化因素的定性、定位、定量的研究涌现出了很多成果,但多集中在理论层面,有关文化因素的具体分析及编写设计则较为薄弱。本文在前人研究的基础上,运用文献研究、典型案例、数据统计、描写分析等方法,以综合教材为切入点,选取了两套使用率较高的综合教材《新实用汉语课本》(5)(6)[刘珣主编.新实用汉语课本[M].北京:北京语言大学出版社,2002.]、《发展汉语》...Affected by the development of the sociolinguistics, functional lingu-stic and social psychology in the last 60’s, the importance of cultural items has been recognized already. As a result of this, a lively discussion was initiated in the Chinese TCSL field about instruction of language and culture in 1980’s. After 3o years’ research, abundant achievements about quantitative, positioning and anal...学位:教育学硕士院系专业:海外教育学院_汉语国际教育硕士学号:2652013115133
Cloning and Preliminary Functional Analysis of OsL-RLK1 and OspPIR2 of Rice
水稻细菌性条斑病是由Xanthomonasoryzaepv.Oryzicola侵染引起的细菌性病害,简称细条病,现已成为威胁我国南方稻区水稻生产的重要病害,也是我国重要的检疫性水稻病害。由水稻细菌性条斑病所引起的产量损失通常为15-25%,严重时可达40-60%。 OsL-RLK1是在水稻细菌性条斑病侵染应答的差异蛋白质组学研究中发现的一个水稻中编码植物受体类蛋白激酶的基因,OspPIR2为病程相关蛋白基因。本实验对OsL-RLK1和OspPIR2基因进行了生物信息学的分析,并根据蛋白序列分析结果,设计PCR引物,得到了目的克隆,为了更好地研究它们的功能,及其在水稻抗病反应中的作用,构建了相...Bacterial Leaf Streak (BLS) caused by the pathogen Xanthomonas oryzae pv. Oryzicola (Xooc.) is one of the major rice diseases in South China. It leads to decreased rice production usually by 15-25%; 40-60% can be serious in prevailing years. OsL-RLK1 is a hypothetical resistant-related gene that has been detected from rice genome based on the study of differential proteomics from rice leaves i...学位:理学硕士院系专业:生命科学学院生物学系_细胞生物学专业学号:2172006115218
Preliminary Study on Regulation of XIOsPR10 Gene Expression in Rice
为探索水稻Pr10蛋白在水稻抗细菌性条斑病中的作用,构建了XIOSPr10基因的植物过量表达载体P1301-XIOSPr10及rnAI表达载体PdS1301-XIOSPr10,通过农杆菌介导分别转化水稻愈伤组织,获得了相应的再生植株。经guS检测和PCr分析,证实XIOSPr10基因以及rnAI片段分别整合到水稻再生植株基因组中;半定量rT-PCr分析显示,过量表达植株中XIOSPr10基因的表达量高于对照,而rnAI转基因植株中XIO-SPr10基因的表达被抑制。In order to study the function of rice XIOsPR10 gene which is related to the plant disease resistant reaction,we constructed plant over expression and RNAi expression vector and integrated into the genome of rice via Agrobacterium tumefaciens EHA105,respectively.The transgenic cultivars were identified by PCR and GUS gene expression detection.The analysis result showed that the transcription level of XIOsPR10 of over-expression transgenic rice was higher than that of control.In construct,XIOsPR10 gene expression was blocked in RNAi transgenic rice.科技部863专题(2007AA10Z132);国家重大科技专项(2008ZX08001-001;2009ZX08009-045B);教育部重点项目(01102
Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak,Xanthomonas Oryzae pv.Oryzicola
水稻细菌性条斑病由XAnTHOM OnAS OryzAEPV.OryzICOlA引起,是目前威胁亚洲稻区水稻生产的重要病害之一。以水稻品种9311为研究对象,通过差异蛋白质组学方法,研究了病菌侵染48 H后水稻叶片的差异表达蛋白质,通过分析比对、质谱分析以及数据库检索,从中选择了7个上调表达的鉴定蛋白,包括4个水稻lrk类基因,2个nbS-lrr类基因和1个Pr-10基因家族成员基因。并设计相应的PCr引物,从水稻CdnA中获得相关基因片段做探针进行nOrTHErn杂交分析,结果显示,上述基因在接种病原菌12 H或48 H后表达量增加,表明这些蛋白质参与了抗病反应。Rice bacterial leaf streak(BLS) caused by the pathogen Xanthomonas oryzae pv.oryzicola(Xooc) is one of the major diseases in Asia.In this paper,the protein differently expressed after infected for 48 h by BLS was studied with rice 9311 as material through proteomic approach.Based on the result of protein blast,fingerprints and data search analysis,seven identified proteins up-regulated were selected including four LRK like genes,two NBS-LRR type genes and one PR-10 gene.Relevant primers were designed to amplify the genes from rice cDNA as probe for Northern blotting.The results showed that the mRNA levels of these genes increased significantly after infected by BLS for 12 h and 48 h,indicating these proteins have participated in disease resistance.国家863计划项目(2007AA10Z132);国家重大科技专项(2008ZX08001-001;2009ZX08009-045B);教育部重点项目(重点01102)资
群体平衡方程在搅拌反应器模拟中的应用
群体平衡方程(population balance equation,PBE)是描述多相流体系中分散相大小与分布随时空变化的通用方程。搅拌反应器内多为多相流体系,考虑到颗粒聚并、破碎等微观机制对颗粒大小、分布、粒数密度等宏观参量的影响,采用PBE对搅拌槽内多相流体系进行数值模拟,可以较准确预测搅拌槽内流场和颗粒的大小与分布。对群体平衡方程在搅拌反应器数值模拟中的应用进行了综述,在简要介绍PBE的基本形式后,讨论了PBE的主要数值求解方法,然后着重介绍近年来采用PBE对搅拌槽内液固沉淀过程、气液及液液体系进行数值模拟的情况,并对今后的研究方向进行了展望
微混合器的研究进展
微混合器作为全微分析系统、生物芯片等的重要部件,引起了学术界广泛的研究与重视。本文从微混合存在的困难、微混合器的原理、分类、加工方法等方面对目前国内外在微混合器方面的研究进行了详细的综述,阐述了目前存在的各类微混合器的优缺点,并提出了自身得到的研究成果,最后就发展前景作了展望
群体平衡方程在搅拌反应器模拟中的应用
群体平衡方程(population balance equation,PBE)是描述多相流体系中分散相大小与分布随时空变化的通用方程。搅拌反应器内多为多相流体系,考虑到颗粒聚并、破碎等微观机制对颗粒大小、分布、粒数密度等宏观参量的影响,采用PBE对搅拌槽内多相流体系进行数值模拟,可以较准确预测搅拌槽内流场和颗粒的大小与分布。对群体平衡方程在搅拌反应器数值模拟中的应用进行了综述,在简要介绍PBE的基本形式后,讨论了PBE的主要数值求解方法,然后着重介绍近年来采用PBE对搅拌槽内液固沉淀过程、气液及液液体系进行数值模拟的情况,并对今后的研究方向进行了展望