Metal Atomic Wires and Single Molecular Junction: Nanoconstruction by STM and Their Quantum Transport

金属原子线和金属-分子-金属结(分子结)等异质结是纳/分子电子学中研究最广泛的基本单元，它们表现出特殊的量子输运性质，如何构建这些分子尺度的异质结、表征其电荷输运行为并且探究调控电导的因素，是该领域当前的重要研究内容。STM裂结法（Scanningtunnelingmicroscopebreakjunction,STMBJ）是目前研究金属原子线和分子结的成熟方法之一，不仅可以快速重复地构建异质结，得到大量的可用于统计分析的电导曲线，也可以对异质结进行I–V表征。本论文主要运用STMBJ技术，从构建金属原子线到金属-分子金属结，研究金属原子线的量子电导和一系列分子结的电荷输运行为；在此基础上引入...Atomic wire and metal/molecule/metal junctions (molecular junctions) are the basic units of molecular electronic. Such heterojunctions show special features of quantum transport. How to build those molecular heterojunctions and to characterize their charge transport and explore the influencing factors are the currently important research contents in the field of molecular electronics. The technolo...学位：理学博士院系专业：化学化工学院_物理化学学号：2052010015366

高含有大豆イソフラボン代謝産物を用いた口腔乾燥症に対する効果とその作用機序の検討

研究成果の概要（和文）：本課題ではエストロゲン様作用を示し抗酸化物質である大豆イソフラボンであるエクオールが唾液分泌に与える影響を検討することを目的とした。対象は中高年の女性、無作為プラセボ対照のクロスオーバー比較試験にてエクオール含有イソフラボンサプリメントを摂取することによる唾液分泌量、唾液・尿中酸化ストレス値、主観的評価の変化を検討した結果は実測値を用いた解析において試験食品群では摂取後に唾液分泌量の増加傾向を認め、尿中のエクオール値は有意に増加した。酸化ストレスマーカーはエクオール摂取により減少する割合が増加した。主観的評価では口や歯の調子が悪いことが気になる、便秘、白髪で有意な改善が認められた。研究成果の概要（英文）：Dry mouth (xerostomia) don’t make enough saliva to keep mouth wet. It is a multi-factorial disease and reduced quality of life (QOL). In addition, the disease has been reported common in menopausal women. This study was conducted to test of equol affect salivary secretion, oxidative stress markers, equol concentrations and subjective measurements. Nine patients were given equol orally at a dosage of 10mg/day for 1 month, and salivary secretion, oxidative stress markers, equol concentrations in the urine and subjective measurements were analyzed before and after treatment. The results showed a significant improvement based on the equol and self-completed questionnaire. Subjective parameters that improved significantly after treatment score, included discomfort in teeth and mouth, constipation and white hair. The saliva flow rate results are increase after 1 month.・2016(平成28)年度 科学研究費補助金 基盤研究(C) 研究成果報告書・研究期間 (年度)：2014-04-01 – 2017-03-31・研究分野：医歯薬

Exonuclease Ⅲ-mediated DNA Molecular Cloning

DNA克隆技术,作为最基本的现代分子生物学实验技术之一,已经成为生物医学研究领域的重要研究手段。传统的分子克隆方法需要经过限制性内切酶酶切和DNA连接酶连接的步骤,是否存在合适的酶切位点和DNA连接酶的效率成为影响克隆的重要限制因素。本文描述了一种由外切核酸酶Ⅲ介导的,以3'-5'外切核酸酶活性和细菌细胞内DNA修复机制为理论基础的DNA分子克隆方法,称为不依赖连接酶的分子克隆(ligation-independent cloning,LIC);证明了该方法的高效性和可靠性,并进一步对酶的用量、反应温度、反应时间、片段载体比例和量等多个参数进行了优化,建立了一种快速、简便和高效的DNA克隆方法。As a basic technique of modern molecular biology,molecular cloning has become an important tool for biomedical research. The traditional method for molecular cloning usually includes two key processes: restriction digestion and ligation. The success of a traditional cloning depends on suitable restriction sites and high ligation efficiency to a great degree. In the present study,we have developed a DNA cloning method mediated by exonuclease Ⅲ. Because this method is based on the 3' to 5' exonuclease activity of exonuclease Ⅲ and the intrinsic DNA repair machinery of bacteria,and is independent of DNA ligase,we designated it as ligation-independent cloning( LIC). We optimized the reaction parameters including the quantity of enzyme for each reaction,reaction time,reaction temperature and the ratio of vector to fragment,and found that this method showed high efficiency and reliability under optimal condition. We have thus established a rapid,simple and efficient DNA cloning method.国家自然科学基金项目(No.81402285)资助~

广东中山水栖寡毛类区系调查

中山市位于珠江三角洲中南部,区内分布江河、溪流、水库、池塘等多种水体,迄今未见对该地区水栖寡毛类区系的研究报道。通过2005年6月和12月两次调查,记述中山市水栖寡毛类31种,隶属4科16属,包括1个国内新纪录种即扇形管盘虫(Aulophorus flabelliger)以及19个广东省新纪录种。区系分析表明,中山有较多的喜温种类

A Portable Environment Parameters Detector Designed Based on MCU AT89C52

本文设计研究了一种便携式环境参数检测仪。该仪器以AT89C52单片机为核心,选用相应的传感器并设计了采集电路,实现对温度、湿度和CO浓度的检测,可显示、查询相关参数,实现人机交互功能;湿度查表子程序中,把划分的湿度小区间视作线性区间,简化MCU计算,提高了系统工作效率。In this paper,a portable environment parameters detector was designed.It mainlydesigned with the adoption of the MCU AT89C52 together with special sensor. It can detect thetemperature, humidity and CO concentration by designing the proper acquisition circuit and itcan also work interactively when displaying, and requesting relative data. To improve the sys-tem's performance, the linearization method was utilized for those small space intervals onthe humidity look-up data designing to simplify the MCU processing.厦门大学科技创新项目(项目编号:20041015

三种湿地植物抗寒性的初步研究

在阶段性降温条件下,研究了三种常用湿地植物黄鸢尾(Iris pseudacorus L.)、花菖蒲(Iris ensata Thunb.)及水芹(Oenabthe javanica)叶片抗寒性相关生理指标(可溶性蛋白质含量、可溶性糖含量、游离脯氨酸含量、丙二醛含量、超氧化物歧化酶活性、过氧化物酶活性、过氧化氢酶活性以及细胞质膜相对透性)的变化。结果表明,冷锻炼使湿地植物通过对抗氧化酶活性及渗透性的调节来提高其机体的抗寒性。低温条件下,黄鸢尾的抗氧化酶活性显著增加,5℃时SOD活性是初始值的5.77倍;花菖蒲保持较高的CAT活性,5℃时其CAT活性是黄鸢尾CAT活性的8.95倍,是水芹CAT活性的3.77倍;水芹叶片中脯氨酸含量明显提高,温度和脯氨酸含量呈负相关(R=-0.755,P=0.050)。综合比较各项生理指标可以得到这三种湿地植物的耐寒性大小顺序依次为:黄鸢尾>花菖蒲>水芹

Methodology proposal for technology transfer through the creation of academic Spin Offs

Orientador: Antonio BatocchioTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia MecânicaResumo: Neste trabalho desenvolveu-se uma metodologia de estímulo a transferência de tecnologia por meio da criação de spin offs acadêmicos que pode ser utilizado por diversas instituições de pesquisa dentro e fora do Brasil. Esta metodologia foi aplicada por três anos consecutivos na UNICAMP por meio de um programa de 4 meses de duração chamado de Desafio Unicamp de Inovação Tecnológica. Pelas três edições do programa passaram mais de 510 participantes, sendo um spin off acadêmico criado e dois em processo final de criação. A metodologia desenvolvida conta com várias etapas: capacitação empreendedora, escolha e análise de tecnologias depositadas pela instituição de pesquisa, desenvolvimento de modelos de negócios para as tecnologias protegidas escolhidas, teste e validação das hipóteses que suportam os modelos de negócios criados, elaboração de uma apresentação final, premiação dos melhores trabalhos e suporte pós-programa aos melhores projetos. Ao final das três edições conclui-se que a metodologia conseguiu estimular a avaliação de mais de 70 tecnologias da instituição de pesquisa mostrando diversos modelos de negócios pelo quais estas poderiam ser exploradas comercialmente. Porém, o estudo também mostrou que para aumentar o número de spin offs acadêmicos será necessário instituir uma política de apoio maior, estruturando principalmente fundos para financiamento dos testes de prova de conceito necessários para o desenvolvimento da empresa em seus estágios iniciais. Além disto, será necessário capacitar melhor os NITs (Núcleo de Inovação Tecnológica) das instituições de pesquisa para que estes possam fornecer apoio e consultoria aos empreendedores em gestão de projetos e aumento da rede de relacionamentos, pois estes foram os pontos que inviabilizaram a maioria das iniciativas empreendedoras iniciadas durante a aplicação da metodologiaAbstract: In this study, it was developed and implemented a method to encourage technology transfer through academic spin-offs that could be used by research institutions in Brazil and around the world. This method was applied for three consecutive years at UNICAMP through four months duration program called "Desafio Unicamp de Inovação Tecnológica". In these three editions, more than 510 participants were trained, one academic spin-off was created and other two are in the final process of creation. The developed method used in the program has multiple stages, as follow: entrepreneurial empowerment, analysis and choice of technologies, development of business models, testing and validation of hypotheses that support the business model created, preparing a pitch and an awarding ceremony. At the end of the three editions, it was concluded that the method could successfully stimulate the evaluation of more than 70 technologies through the creation of more than 150 business models. However, the study also indicated that if Brazil wants to increase the number of academic spin-offs it needs to create a policy to support the creation of funds for financing the proof of concept tests, so necessary for the development of an early stage technology company. Additionally, further personal development is needed in order to enable the research institutions¿ NIT personnel (Technology Innovation Nuclei) to support and advise entrepreneurs in project management and increase their business network. Errors in these supports and advices were shown to contribute in the failure of most entrepreneurial endeavors carried on this studyDoutoradoMateriais e Processos de FabricaçãoDoutor em Engenharia Mecânic

デザインの類似性の分類法に関する感性工学的アプローチ

The cluster analysis has been applied as a practical method to classify similarities of various designs, including package design, where the distance among the two coordinates evaluated numerically, from the image level of design factors, provides the criterion to classify the similarity. The coordinates have definite values. However, from the view-point of Kansei Engineering, it is more rational to define it on fuzzy logic on account of the fuzziness implicated in the image level of design factors. This paper proposes a new approach to classify the similarity of design, using the coordinates expressed with the expectation and standard deviation of the image level of design factors obtained from fuzzy logic