EFFECTS OF TACHYPLESIN AND N-SODIUM BUTYRATE ON THE MORPHOLOGY AND ULTRASTRUCTURE CHANGES OF HUMAN GASTRIC ADENOCARCINOMA CELLS LINE BGC-823

目的以正丁酸钠为平行对照,比较观察鲎素和癌细胞诱导分化物对人胃癌细胞形态与超微结构变化的影响。方法采用光镜、扫描电镜与透射电镜,观察经2.0 mg/L鲎素、2.0 mmol/L正丁酸钠和1.0 mg/L鲎素+1.0 mmol/L正丁酸钠组合处理的人胃腺癌BGC-823细胞。结果光镜观察显示,鲎素和正丁酸钠以及鲎素+正丁酸钠处理的BGC-823细胞形态均较为一致,细胞体积增大、趋于扁平铺展状态,细胞核质比例减小,细胞核形状较圆整,核仁数量减少。扫描与透射电镜观察可见经鲎素、正丁酸钠及其组合处理的BGC-823细胞均出现细胞表面微绒毛稀少,细胞边缘丝状伪足减少,片状伪足增多;细胞核形态较规则,核内异染色质减少,常染色质增多:细胞质内线粒体增多,结构较为一致,高尔基复合体呈较为典型发达状态,并出现粗面内质网增多和多聚核糖体减少等变化。结论鲎素具有与正丁酸钠等诱导分化物相似的改变人胃癌细胞形态与超微结构恶性表型特征的作用,和与诱导分化物协同加成的诱导分化效果。Objective To compare the morphology and ultrastructure changes of human gastric adenocarcinoma cell line treated by tachyplesin and n-sodium butyrate. Methods Light microscope,scanning electron microscope and transmission electron microscope were used to observe human gastric adenocarcinoma cell line BGC-823 treated by 2.0?mg/L tachyplesin,2.0?mmol/L n-sodium butyrate and 1.0?mg/L tachyplesin in combination with 1.0?mmol/L n-sodium butyrate respectively. Results Light microscope observation showed that BGC-823 cells treated by tachyplesin,n-sodium butyrate and tachyplesin+n-sodium butyrate possessed the similar cell modality as follow: the volume of cell increased,the shape of cell became flat and outspread,nucleo-cytoplasmic ratio decreased,the shape of nucleus was rounde,the number of nucleolus decreased.Scanning electron microscope and transmission electron microscope observation showed that,in the BGC-823 cells which were treated with tachyplesin,n-sodium butyrate and tachyplesin in combination with n-sodium butyrate respectively,microvilli and filopodia reduced,sheed pseudopodia increased,the shape of nucleus became regular,heterochromatin decreased while euchromatin increased,the number of mitochondria increased and its structure appeared consistent,Golgi complex turned to be typical,the amount of rough endoplasmic reticulum increased and that of polyribosome decreased.Conclusion All of these results showed that tachyplesin possessed the similar effects to n-sodium butyrate on changing morphology and ultrastructure of human gastric adenocarcinoma cells and possessed an additive role of inducing tumor cells to differentiate cooperatively with n-sodium butyrate to induce carcinoma cell differentiation.国家自然科学基金资助[30170724

Clinical analysis of perimenopausal women presenting initially with dry eye symptom

目的探讨围绝经期妇女的干眼症状与干眼病诊断的相关性。方法分析56例具有干眼症状的围绝经期妇女眼部检查的临床资料,观察主诉为干眼症状患者的干眼病确诊率及其关系。结果56例以干眼症状为主要表现的围绝经期妇女,确诊水液缺乏型干眼17例(30.4%),蒸发过强型干眼12例(21.4%),混合型干眼8例(14.3%);不能诊断干眼病的19例(33.9%),最终诊断为围绝经期综合征。结论围绝经期妇女干眼病确诊率较高(66.1%),但部分干眼症状可以只是围绝经期综合征的精神神经症状的一种表现,临床诊断干眼应慎重。 【英文摘要】 Objective Diagnosis and treatment of dry eye are important for improving living quality.Present paper was to investigate the correlation between dry eye manifestations and diagnosis of dry eye disease in perimenopausal women.MethodsThe clinical data of 56 perimenopausal women presenting initially with dry eye symptoms including ocular symptoms,slit-lamp examination,tear film break-up time,basal and reflex Schirmer test,vital staining(fluorescence and rose bangle),and meibomian gland dysfunction examination ..

Fish species composition and community pattern in the continental shelf of northwestern South China Sea

根据2006—2007年在南海西北部陆架区海域进行的4航次底拖网调查数据,对该海域鱼类的种类组成和群落格局进行分析。调查共采获鱼类262种,隶属于20目102科。以冬季出现种类数最多,为166种;夏季和秋季次之,分别为161种和155种;春季最少,为135种。运用聚类分析和非度量多维标度(nMdS)方法分析了南海西北部陆架区鱼类群落结构的空间分布。研究表明,该海域鱼类可划分为5个群落,分别为粤西沿岸群落(群落Ⅰ)、海南岛东南沿岸群落(群落Ⅱ)、海南岛东部深海群落(群落Ⅲ)、过渡群落(群落Ⅳ)和混合群落(群落V)。AnOSIM检验表明,各群落间鱼类组成的差异极为显著(r=0.847-0.939,P<0.001)。鱼类群落格局的变化与海流和水深关系密切,有较明显的沿水深梯度分布的规律。咸淡水鱼类在雷州半岛东部的冷涡区域,形成群落Ⅰ;南海暖流控制区域内的深海鱼类,形成群落Ⅲ;在南海暖流最为强劲的冬季,研究区域内大部分鱼类集结为混合性群落V;琼东南沿岸的上升流区内形成群落Ⅱ;沿岸流强劲的夏、秋季,在沿岸流和上升流交汇处,形成过渡性群落Ⅳ。To understand fish species composition,community pattern,dominant species and seasonal variation in the continental shelf of northwestern South China Sea,four otter trawl surveys were conducted in January and August 2006,and April and November 2007,representing winter,summer,spring and autumn,respectively.Sixteen stations were assigned in each otter trawl survey along the coastlines from eastern Leizhou Peninsula to southeastern Hainan Island.Fish collection and data analysis followed the National Marine Survey Standards(GB/T12763.6—2007).Totally 262 fish species,from 102 families and 20 orders,were collected in the four seasonal surveys.The results revealed that the highest species diversity was in winter with 166 species recorded and the lowest was in spring with 135 species recorded;161 and 155 species were recorded in summer and autumn,respectively.The Order Perciformes was the most important fish group in the continental shelf of northwestern South China Sea;the highest species diversity recorded was the Perciformes in all four seasons,and 40 out of 72 species recorded year-round belonged to the Perciformes.Species found in all four seasons contributed to 80.34% of total fish biomass.Ninety-one species found only in a single season contributed to 1.62% of total fish biomass.The spatial pattern of fish communities was analyzed using the hierarchical clustering and non-metric multi-dimensional scaling(NMDS),five fish groups were indentified.The Group Ⅰ,recorded in all four seasons,was located in the coastal waters of western Guangdong Province and consisted of 3 or 4 stations.The scale and the distribution area of the Group Ⅰ were stable among seasons.The Group Ⅱ,recorded in spring,summer and autumn,was located in the coastal waters of southeastern Hainan Island and consisted of 6—9 stations.The scale and the distribution area of the Group Ⅱ had seasonal variation.The Group Ⅲ,recorded in spring,summer and autumn,was located in the deep waters of eastern Hainan Island and consisted of 4 stations.The scale and the distribution area of the Group Ⅲ were stable.The Group Ⅳ as a transitional community,recorded in summer and autumn only,was located in the deep waters of eastern Hainan Island and consisted of 1—3 stations.The scale and the distribution area of the Group Ⅳ were small and changed largely with season.The Group Ⅴ as a mixed community,recorded in winter only,was also located in the deep waters of eastern Hainan Island and consisted of 13 stations.The scale and the distribution area of the Group Ⅴ were the largest among the 5 groups.Based on the analysis of similarities(ANOSIM),the results showed the significant differences(R=0.847-0.939,P <0.001) in fish community pattern for all four seasons.Although the station combinations in each fish community had seasonal variation,the correlation coefficients on the community similarities between spring and summer,between summer and autumn and between autumn and winter were 0.780,0.630 and 0.777,respectively.The variations of fish community pattern are closely related to ocean currents and depth.The brackish fishes distributed in the cold eddy area of eastern Leizhou Peninsula and contributed to the Group Ⅰ.The deep sea fishes along the South China Sea Warm Current in eastern Hainan Island contributed to the Group Ⅲ.In winter with the strongest South China Sea Warm Current recorded in the deep waters of eastern Hainan Island,the area was dominated by fishes from the mixed Group Ⅴ.Because of the upwelling in the coastal waters of southeastern Hainan Island the area was dominated by fishes from the Group Ⅱ.In summer and autumn,the coastal current was strong and mixed with the upwelling in the deep waters of eastern Hainan Island;therefore,the area was dominated by fishes from the transitional Group Ⅳ.This study provides scientific evidences for further sustainable fisheries development and conservation in the continental shelf of northwestern South China Sea.我国近海海洋综合调查与评价专项(908-01-ST08); 中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2009TS08;2010YD01和2010YD10); 农业部近海渔业资源调查项目(070404); 海洋公益性行业科研专项(201005012); 广东省海洋渔业资源综合评价资助项目(GD908-02-05

附属幼稚園における保育実践の見直し－「あそびまつり」の取り組み－

幼稚園の保育を捉えなおす試みについての研究．子どもたちの遊びが途中で途切れることがないような保育を計画し実践した．幼稚園の園舎，園庭のどこででも遊ぶことができる日を設定し，「あそびまつり」と名付けた．子どもたちは，その環境の下で自己発揮するとともに，保育者にとっても多様な学びがあった．子ども理解や関りのあり方がより深く捉えられるようになった．保育者の専門性の自覚が深まったなどである．積極的な保育の見直しにより，カリキュラムマネージメントがなされた．キーワード：保育の見直し，制限の少ない遊び環境，保育者の認め合

中国海及邻近区域碳库与通量综合分析

中国海总面积约470万平方公里,纵跨热带、亚热带、温带、北温带等多个气候带.其中,南海北依\"世界第三极\"青藏高原、南邻\"全球气候引擎\"西太平洋暖池,东海拥有全球最宽的陆架之一,跨陆架物质运输显著,黄海是冷暖流交汇区域,渤海则是受人类活动高度影响的内湾浅海.中国海内有长江、黄河、珠江等大河输入,外邻全球两大西边界流之一的黑潮.这些鲜明的特色赋予了中国海碳储库和通量研究的典型代表意义.文章从不同海区（渤海、黄海、东海、南海）、不同界面（陆-海、海-气、水柱-沉积物、边缘海-大洋等）,以及不同生态系统（红树林、盐沼湿地、海草床、海藻养殖、珊瑚礁、水柱生态系统等）多层面对海洋碳库与通量进行了较系统地综合分析,初步估算了各个碳库的储量与不同碳库间的通量.就海气通量而言,渤海向大气中释放CO2约0.22Tg Ca-1,黄海吸收CO2约1.15Tg Ca-1,东海吸收CO2约6.92～23.30Tg Ca-1,南海释放CO2约13.86～33.60Tg Ca-1.如果仅考虑海-气界面的CO2交换,中国海总体上是大气CO2的\"源\",净释放量约6.01～9.33Tg Ca-1.这主要是由于河流输入以及邻近大洋输入所致.河流输入渤黄海、东海、南海的溶解无机碳（DIC）分别为5.04、14.60和40.14Tg Ca-1,而邻近大洋输入DIC更是高达144.81Tg Ca-1,远超中国海向大气释放的碳量.渤海、黄海、东海、南海的沉积有机碳通量分别为2.00、3.60、7.40、7.49Tg Ca-1.东海和南海向邻近大洋输送有机碳通量分别为15.25～36.70和43.39Tg Ca-1.就生态系统而言,中国沿海红树林、盐沼湿地、海草床有机碳埋藏通量为0.36Tg Ca-1,海草床溶解有机碳（DOC）输出通量为0.59Tg Ca-1;中国近海海藻养殖移出碳通量0.68Tg Ca-1,沉积和DOC释放通量分别为0.14和0.82Tg Ca-1.总计,中国海有机碳年输出通量为81.72～103.17Tg Ca-1.中国海的有机碳输出以DOC形式为主,东海向邻近大洋输出的DOC通量约15.00～35.00Tg Ca-1,南海输出约31.39Tg Ca-1.综上,尽管从海-气通量看中国海是大气CO2的\"源\",但考虑了河流、大洋输入、沉积输出以及微型生物碳泵（DOC转化输出）作用后,中国海是重要的储碳区.需要指出的是,文章数据是基于中国海各海区碳循环研究报道,鉴于不同研究方法上的差异,所得数据难免有一定的误差范围,亟待将来统一方法标准下的更多深入研究和分析.国家重点研发计划项目(编号:2016YFA0601400);;国家自然科学基金项目(批准号:91751207、91428308、41722603、41606153、41422603);;中央高校基础研究项目(编号:20720170107);;中海油项目(编号:CNOOC-KJ125FZDXM00TJ001-2014、CNOOCKJ125FZDXM00ZJ001-2014)资

Combustion Synthesis and Electrochemical Properties of the Small Hydrofullerene C50H10

通讯作者地址: Xie, SY (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 3. Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China 电子邮件地址: [email protected]; [email protected] hydrofullerene C50H10 is synthesized by low-pressure benzeneoxygen diffusion combustion. The structure of C50H10 is identified through NMR, mass spectrometry, and IR and Raman spectroscopy as a D5h symmetric closed-cage molecule with five pairs of fused pentagons stabilized by ten hydrogen atoms. UV/Vis and fluorescence spectrometric analyses disclose its optical properties as comparable with those of its chloride cousin (C50Cl10). Cyclic and square-wave voltammograms reveal that the first reduction potential of C50H10 is more negative than that of C50Cl10 as well as C60, with implications for the utilization of C50H10 as a promising electron acceptor for photovoltaic applications.973 projects 2011CB935901 NSFC 21031004 21021061 2077310

振動感測器結構

[[abstract]]本發明涉及一種振動感測器結構，特別係指一種利用布拉格光纖光柵特性及原理作為振動感測之振動感測器結構，該振動感測器包含有一底座、一簧片座、一質量塊及一預拉板，其中質量塊係利用簧片座設於底座上，且質量塊具有一向上斜伸懸臂，又質量塊於懸臂頂面與預拉板頂面之間分別利用一具彈性作用的夾壓塊來夾掣光纖，透過上述的設計，不僅可有效簡化結構，且可克服如利用鎖固件直接鎖掣所產生的光纖斷裂問題，且可調整其靈敏度