Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c

Corresponding author. Email: [email protected]; Tel: +86-592-2189663.[中文文摘]金纳米粒子(AuNPs)的颜色会随着细胞色素c(Cytc)构象变化而发生较大的变化,作者在改变原测定流程的基础上利用这种有规律的变化研究和测定了H+和L-半胱氨酸(L-Cys)对Cytc的构象变化.实验中分别加入pH=1-13的Cytc,可以使AuNPs显示青、蓝、紫、红等明显不同的颜色,从而可以利用AuNPs比色快速测定不同pH值的Cytc构象变化.在pH=7时,当附加L-Cys浓度从低浓度变化到高浓度时,AuNPs颜色逐渐从紫色变化到蓝色、青色,从而实现利用AuNPs比色测定由L-Cys所引起Cytc构象变化.圆二色(CD)光谱证实了pH=1-13和不同浓度L-Cys下的Cytc构象变化.借助紫外-可见吸收光谱和扫描电子显微镜(SEM)进一步明确了加入Cytc后AuNPs的不同聚集状态与其颜色变化的关系.[英文文摘]The colors of Au nanoparticles(Au NPs) change along with conformational changes in cytochrome c(Cyt c).We exploited this property for the colorimetric detection of Cyt c conformational changes induced by H + and L-cysteine(L-Cys).We improved the conventional procedure for this detection.After the addition of Cyt c within different pH values,the Au NPs are either cyan,blue,purple or red.This indicates that the Au NPs can be applied to the rapid colorimetric detection of pH-induced conformational changes in Cyt c conformational changes caused by interaction with L-Cys. The conformational changes of Cyt c were verified by circular dichroism (CD) spectroscopy. The relationship between the aggregation states and colors of the Au NPs after the addition of Cyt c was characterized by UV-Vis absorption spectroscopy and scanning electron microscopy (SEM).国家自然科学基金(20603027,20423002)资助项

胸腺素原体内抑制小鼠肝癌细胞H22的研究

根据PArkIn等[1]报道,2000年全球新发癌症病例为1 010万例,死亡620万。在fErlAy等[2]报道中最常见的癌症中致死率排列第三是肝癌。在我国肝癌的死亡率占全部恶性肿瘤死亡的18.8%,仅次[3]厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103);福建省医药卫生创新项目(2009-CXB-51)资

胸腺素原体内抑制小鼠肝癌细胞H22 的研究

作者简介: 蔡报伟( 987年－ ) ，男，在读硕士，主要从事免疫学及基因工程药物研究，E-mail: ncuskcaibaowei@163. com; 通讯作者及指导教师: 周克夫(1966 年－ ) ，男，博士，副教授，主要从事免疫学及基因工程药物研究，E-mail: [email protected]。厦门市科技项目(3502Z20083004); 国家“973”项目(2007CB513103); 福建省医药卫生创新项目(2009-CXB-51)资

Electrochemical Behavior of Redox Proteins on ZnO Nanorod-Modified Electrodes Prepared by Electrodeposition

Corresponding authors. ZHOU Jian-Zhang, Email: [email protected]; Tel: +86-592-2189663.[中文文摘]采用恒电位阴极还原法在金电极表面一步修饰ZnO纳米棒,制备成ZnO纳米棒修饰电极.扫描电子显微镜(SEM)和X射线衍射(XRD)结果显示制得的ZnO为直径约100nm的六棱柱状纤锌矿晶体纳米棒.使用ZnO纳米棒修饰的金电极研究细胞色素c的直接电化学行为,结果表明:ZnO纳米棒修饰的金电极能有效探测到细胞色素c的铁卟啉辅基在不同价态下的电化学行为;细胞色素c吸附后,ZnO纳米棒修饰的金电极对过氧化氢的电流响应呈现良好的线性关系.[英文文摘]We successfully prepared ZnO nanorod-modified Au (ZnO nanorod/Au) electrodes using one-step cathodic electrodeposition. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to characterize the morphology and crystal phase of the ZnO nanorods. The data showed that the ZnO nanorods were wurtzite type crystals with a hexagonal rod shape and a diameter of about 100 nm and that the ZnO nanorods were arranged well on the surface of electrodes. These ZnO nanorod-modified electrodes were able to detect direct electron transfer from cytochrome c (cyt c). Cyclic voltammograms showed that the direct electron transfer of cyt c with heme iron in different valence states was easily achieved by the ZnO nanorod/Au electrode. Data of amperometric responses demonstrated that a linear amperometric response to hydrogen peroxide was observed on the ZnO nanorod/Au electrodes after adsorbing cyt c.国家自然科学基金(20603027,21021002,20973134); 国家高技术研究发展专项经费项目(2009AA03Z327)资

Influence of Proportion of Treg Cells and NKG2D-positive Cells by Recombinant ProTαin Mice Bearing Liver Cancer

为观察不同时期应用重组胸腺素α原(ProTα)药物干预下肝癌荷瘤小鼠的抑瘤率,并研究其对Treg细胞和NKG2D阳性细胞的影响,将36只昆明小鼠随机分成空白组、荷瘤组、药物干预组,H22小鼠肝癌细胞皮下移植建立荷瘤小鼠模型,腹腔注射重组ProTα,观察7和14d后肝癌荷瘤小鼠的抑瘤率,并检测外周单个核细胞中调节性T细胞(Treg细胞)和NKG2D阳性细胞的比例.结果表明:移植瘤7d后,药物干预组和荷瘤组瘤质量对比无显著差异;而移植瘤14d后,药物干预组瘤质量较荷瘤组有显著减小.荷瘤14d后,荷瘤组较空白组Treg细胞比例升高,NKG2D阳性细胞比例下调,差异显著;而不论是早期药物干预组还是进展期(即移植瘤7d后)药物干预组,Treg细胞比例均显著降低,NKG2D阳性细胞显著升高.由此可见,重组ProTα能够抑制肝癌荷瘤小鼠肿瘤生长,且早期、长期用药效果更好,相关作用机制涉及下调Treg细胞数量从而抑制肝癌细胞免疫逃逸,并上调NKG2D阳性细胞数量从而提高其介导的抗肿瘤效应.To observe the tumor inhibition rate by recombinant ProTαin liver cancer-bearing mice model,and to study the influence of the proportion of Treg cells and NKG2D-positive cells,36 Kunming mice were randomly divided into control group,tumor-bearing group and drug intervention group.Then a tumor-bearing mice model was established by transplanting H22 cells subcutaneously.By medicating recombinant ProTαthrough intraperitoneal injection,we observed the tumor inhibition rate after 7days and 14 days,and detected the proportion of Treg cells and NKG2D-positive cells in PBMCs.Results showed that the difference in tumor mass between the tumor-bearing group and the drug intervention group was not significant after bearing tumor for 7days.However,after bearing tumor for 14 days,the difference in tumor mass was significant.Additionally,the proportion of Treg cells increased and the number of NKG2D-positive cells declined significantly in the tumor-bearing group after bearing tumor for 14 days.No matter 14 days intraperitoneal injection of recombinant ProTαor drug intervention from the advanced stage,the proportion of Treg cells declined and the number of NKG2D-positive cells increased significantly.The results suggest that recombinant ProTαinhibits tumor growing,and the early and long-term drug intervention benefits the most.It is believed that the putative mechanism is related to that recombinant ProTαdown-regulates the proportion of Treg cells,inhibiting the immune escape of hepatocellular carcinoma cells,and up-regulates the number of NKG2D-positive cells,improving the NKG2D-mediated anti-tumor effect.福建省自然科学基金(2013J01383);; 福建省医学创新课题(2009-CXB-51

液压式材料试验机改为疲劳试验机的一种简易方法

疲劳研究是许多工业部门迫切需要开展的课题,但是要开展疲劳方面的研究,必须有疲劳试验设备,疲劳试验机就是最基本的设备之一。现在市场上销售的疲劳试验机,价格昂贵,一般都在十几万元至一百多万元。许多小企业和厂家都难以购买,而这些企业和厂家却有进行静力试验的材料试验机。如果将材料试验机改装为疲劳试验机,既可以充分发挥试验机的效率,又可以开展疲劳试验,节省大量的资金

Germination strategies of Suaeda salsa at different temperature and under different salt stress

为了研究黄河三角洲优势种盐地碱蓬在不同胁迫环境条件下的萌发策略,分别在不同温度、盐度、碱度以及海水原溶液条件下,进行了室内萌发实验,并且测量了其幼苗体内的Na+和K+含量。结果表明,盐地碱蓬种子发芽所需要的积温和最低温度分别为24.57℃.d和0.62℃,最适发芽温度为20℃~35℃,在温度5℃~40℃下均表现出较高的发芽率而且5℃~35℃下发芽速度随温度升高而显著增加。盐地碱蓬具有较高的耐盐性,当盐浓度达到500 mmol.L-1时,发芽率均高于50%,并且在100%海水溶液浓度下发芽率也能达到38%,高盐条件下未萌发的种子转移到淡水中,均表现出较高的复萌率。盐地碱蓬幼苗体内Na+含量随盐度(NaCl溶液浓度)升高而显著增加,而K+含量在该盐度下差异不显著;幼苗体内Na+、K+含量在高碱度(200和300 mmol.L-1NaHCO3)中均显著低于其在低碱度(100 mmol.L-1NaHCO3溶液)中的含量,说明碱胁迫对幼苗生长产生了显著性影响;Na+、K+含量均随着海水溶液浓度增加而显著增加。因此,盐地碱蓬种子萌发的广温性、高耐盐性、高盐环境中的种子高存活率以及幼苗的较强的耐盐能力是盐地碱蓬种群在黄河三角洲适应滨海盐碱湿地复杂环境的主要生存策略。

Intensity Change of Human Disturbance and Its Response to Landscape Pattern in the Yellow River Delta

The coastal wetlands of the Yellow River Delta is the most integral, broadest and youngest wetland ecosystem in the warm temperate zone of China. Also, it has unique ecological landscape as well as important ecological service functions. With the increase in population and the intensification of high- intensity development and construction activities, the sensitivity/fragility of the area is highlighted, which poses a great threat to the sustainable development of coastal areas. Aiming at the unique geographic location and resource characteristics of the Yellow River Delta, based on RS and GIS, the hemeroby model as well as the man-made ditches density were used in this study to intuitively reveal the temporal and spatial characteristics of human disturbance intensity in 1995,2004 and 2015 at the regional and local scales. The response of the wetland landscape pattern to human interference activities was explored as well. A grid analysis method was used to calculate the hemeroby index and man- made ditches density. The data source included the following: Landsat TM、ETM~+ and OLI Remote Sensing Data (30 m resolution) in 1995、2004、2015, SPOT image of 2004(5m resolution), GF-1 image of 2015 (2 m resolution) and topographic map. This study aims to provide decision support for ecological regulation and spatial control on human activities in coastal wetlands of the Yellow River Delta. The results showed that: (1) From 1995 to 2015,both the hemeroby index and the density of artificial ditches in the Yellow River Delta had increased significantly, and its spatial distribution showed a trend of expansion from the southwest to the east and north, and from inland to coastal areas; (2) With the increase of human activities, the area of natural wetlands in the study area have been decreased from 3232.11km~2 in 1995 to 2165.48km2 in 2015,the landscape diversity and spatial heterogeneity increased, ecological connectivity weakened, the plaque shape tended to be regular and simplistic. (3) The response of landscape pattern to hemeroby index varies regionally and temporally. In the Kenli Agricultural Area, Hekou Agricultural Area and Shengli Oil Field Area, where the intensity of human interference activities had been relatively high, the intensity increased significantly, and the regional landscape pattern changed obviously in different phases. In the Yiqianer Management Station and the Yellow River Estuary Management Station area, where the intensity of human interference activities had been relatively low, due to the increase in the density of artificial ditches, the landscape pattern had presented a decreasing trend in landscape diversity, patch complexity and ecological connectivity from 2004 to 2015. (4) The indices of hemeroby and the density of man-made ditches supplement each other and confirm each other, which can reflect the degree of human disturbance activities in the Yellow River Delta more comprehensively and objectively

硫酸化多糖在制备抗凝血活性药物中的应用

本发明涉及生物医药，是一种用于治疗和预防血栓疾病的药物，具体地说是硫酸化多糖在制备抗凝血活性药物中的应用。本发明优点为：菊粉来源丰富，硫酸化菊粉衍生物的制备条件容易操作，可适合大规模生产；抗凝血效果显著，发展前景广阔，意义深远。带填