Study of the PTHrP and Notch pathways on the Proliferation of epiphysis stem cells

目的：探索PTHrP、Notch双信号通路对骨骺干细胞增殖的调控规律及二者之间的相互作用。 方法：体内实验：取24h内新生大鼠股骨体外器官培养，分别用PTHrP信号通路激活剂PTHrP（1~34）和PTHrP受体竞争抑制物PTHrP（7~34），Notch信号通路激活剂Jagged1/Fc和抑制剂DAPT处理，空白对照加PBS缓冲液，培养72h后收集标本行HE染色比较骨骺干细胞层在骨骺全长中比值和BrdU染色检测细胞增殖情况，并用免疫组化染色方法检测当PTHrP信号通路被干预时Notch信号通路配体Jagged1、受体NICD蛋白的表达情况，及当Notch信号通路被干预时PTHrP蛋白的表达...Objectives: To investigate the regulation of PTHrP and Notch signaling pathway on the proliferation of epiphysis stems cells, and to understand the relationship between PTHrP and Notch pathway. Methods: An organ culture system was used to cultivate femurs of SD rat in 24h after birth. PTHrP(1~34) was used as the activator of the PTHrP signaling passway and PTHrP(7~34) as the antagonist of PTH/P...学位：医学硕士院系专业：医学院临床医学系_外科学学号：2452008115345

一期翻修术治疗膝关节假体周围感染的疗效分析

目的探讨一期膝关节翻修术治疗膝关节假体周围感染的安全性及有效性。方法选取2015年1月～2018年6月厦门大学附属福州第二医院因膝关节假体周围感染接受一期膝关节翻修术的患者28例。收集患者的人口统计学资料、感染病原,并回顾性分析患者术前及术后KSS、HSS与OKS评分及影像学资料,评估膝关节假体周围感染接受一期膝关节翻修术的安全性及临床疗效。结果 28例接受一期膝关节翻修术的患者,男14例,女14例,平均年龄(65.3±10.1)岁,截止到末次随访,除1例患者死亡(死于心肌梗死)失访外,其余27例患者平均随访25.7个月(13～39个月),未发现假体松动及假体周围感染复发。患者术前KSS评分平均(34.19±13.00)分,末次随访时平均(76.37±16.46)分;术前HSS评分平均(36.03±13.39)分,末次随访时平均(71.24±16.39)分;术前OKS评分平均(16.62±3.47)分,末次随访时平均(27.43±6.02)分,差异有统计学意义(P <0.05)。结论在选择合适适应证情况下,膝关节置换术后假体周围感染的患者通过一期膝关节翻修术可以有效控制感染并重建膝关节功能。福建省自然科学基金项目(2017J01333

The relation between the fluidity of cement paste without bleeding and concrete-slump-lost controlling ability

　在研制合成氨基磺酸系高效减水剂的过程中,对比了合成产品的无泌水水泥净浆在2h内的流动度和掺入了合成产品的混凝土在2h内的坍落度损失值。结果表明,利用合成产品的无泌水水泥净浆流动度的保持性能可以定性判定合成产品在保持混凝土坍落度方面的能力。采用这一方法,在检测合成产品性能的过程中,可以大大减少后期混凝土性能检测的试验量。This paper mainly discusses the performances of cement paste applying Point-AH superplasticizer of sulfonic acid series.And the fluidity of cement paste without bleeding in two hours and the slump loss of concrete in two hours are studied.The results show that the ability of the synthesis production to control the slump loss can be qualitatively determined through the ability to keep the fluidity of cement paste without bleeding.By this method,concrete experiments can be largely reduced in the performance testing of the synthesis production

人骨髓间充质干细胞分离培养方法的改进

背景:当前分离培养骨髓间充质干细胞方法的有多种,但在外科手术中进行获取的方法仍然较少,而外科分离所得细胞对于相应疾病的具有揭示原因和提供研究线索的价值。目的:在骨科手术中以直接贴壁差速贴壁分离培养纯化与鉴定骨髓间充质干细胞。方法:在骨科关节置换等大手术中吸取少量人骨髓血,采用直接贴壁法分离骨髓间充质干细胞,在贴壁后36～48h行洗盘处理,并通过长期体外培养,特定培养基自身的筛选作用对细胞进行筛选,当细胞生长达到足量时进行鉴定。结果与结论:采用直接贴壁法能够在长骨髓腔内混合血中分离培养骨髓间充质干细胞并形成集落,其形态学表现和表面分子经鉴定符合骨髓间充质干细胞特点,能够获得符合要求的人骨髓间充质干细胞,从而进行后续实验

Effects of Serum Medicated with Duhuo Jisheng Decoction on the Induced Degeneration of Articular Chondrocytes in Vitro Culture Based upon “caveolin-p38MAPK” Signal Pathway

目的:观察独活寄生汤含药血清对兔退变软骨细胞“CAVEOlIn-P38MAPk“信号通路的调控作用,探讨独活寄生汤治疗骨关节炎的作用机制。方法:将20只3月龄新西兰兔随机分为生理盐水组(空白血清组)和独活寄生汤组(含药血清组),每组10只。分别在24 H、36 H、48 H不同采血时间点采集独活寄生汤含药血清和空白血清,将5%、10%、15%、20%不同浓度两种血清作用于体外培养第2代软骨细胞,确定含药血清最佳干预条件;建立体外退变软骨细胞模型,分别给予独活寄生汤含药血清(含药血清组)和空白血清(空白血清组)干预36 H,收集软骨细胞,运用WESTErn blOT法检测血清干预后退变软骨细胞CAVEOlIn-1、P38、P-P38蛋白表达,rT-PCr法检测血清干预后退变软骨细胞白细胞介素(Il)-1β、肿瘤坏死因子(Tnf)-α、基质金属蛋白酶(MMP)-3、MMP-13、CAVEOlIn-1 M rnA表达。结果:浓度为15%的36 H采血时间点含药血清的促增殖作用最明显;退变软骨细胞中存在“CAVEOlIn-P38MAPk“信号通路的激活,独活寄生汤含药血清可抑制CAVEOlIn-1、P-P38蛋白表达及Il-1β、Tnf-α、MMP-3、MMP-13、CAVEOlIn-1 M rnA的表达,差异有统计学意义(P<0.05)。结论:独活寄生汤能通过抑制“CAVEOlIn-P38MAPk“信号通路的激活及其下游效应分子,从而有效抑制软骨细胞凋亡。Objective:To observe the effects of serum medicated with Duhuo Jisheng Decoction on the induced degeneration of articular chondrocytes in vitro culture based upon "caveolin-p38MAPK" signal pathway and probe the mechanism of Duhuo Jisheng Decoction in the treatment of osteoarthritis.Methods:20 March old New Zealand rabbits were randomly divided into normal saline group(blank serum group)and Duhuo Jisheng Decoction group(drug containing serum group),10 rats in each group.The second generation of cartilage cells was cultured in vitro using different time points of sample collection such as 24 h,36 h and 48 h,serums medicated with 5%,10%,15% and 20% concentrations of Duhuo Jisheng Decoction and blank serums.The best intervention time of medicated serum was determined,which was then used to deal with the degenerating chondrocytes.The degenerated cartilage cell models in vitro were established,which were intervened by the serum medicated with Duhuo Jisheng Decoction(drug containing serum group)and the blank serum(blank serumgroup)after 36 h,collecting chondrocytes.The Western Blot method was used to detect the expression of degenerated cartilage cells caveolin-1 and p-p38 protein after serum intervention.The RT-PCR method was usedto detect the expression of degenerated cartilage cells IL-1β,TNF-α,MMP-3,MMP-13 and caveolin-1 m RNA after serum intervention.Results:The concentration of 15% and 36 h sampling time point serum on the proliferation;the activation of "caveolin-p38MAPK" signaling pathway in the degeneration of cartilage cell,Duhuo Jisheng decoction containing serum can inhibit the expression of caveolin-1,p-p38 protein expression and IL-1β,TNF-α, MMP-3,MMP-13,caveolin-1,m RNA,the difference was statistically significant(P < 0.05).Conclusion:Duhuo Jisheng decoction can inhibit the "caveolin-p38MAPK" signal pathway and its downstream effector molecules, which can effectively inhibit the apoptosis of cartilage cells.国家自然科学基金资助项目(81302986); 福建省卫生厅青年科研课题资助计划项目(2012-2-69); 福建省自然科学基金(2013J01389); 福州市卫生系统科技计划(2013-S-wq6

基于FPGA的CSR高频控制系统相位求解模块设计

CSR高频控制系统需要对高频正弦波激励信号的幅度、相位、频率进行稳定控制。相位arctan(Q/I)求解是必不可少的模块。论述了一种新的基于FPGA平台和对称查找表法(SBTM)的求解相位arctan(Q/I)的方法,做了详细的理论分析,给出了具体实现的代码和结果。该模块精度高,消耗资源少,可直接应用于CSR高频控制系统

直接前侧入路全髋关节置换是否导致髋臼前壁偏心磨挫?（英文）

背景:目前有学者认为直接前侧入路行髋关节置换术会导致髋臼前壁的过度磨挫,但是缺少确切的临床证据来证实。目的:试验拟通过髋臼CT影像测量,观察比较直接前侧入路与后外侧入路行全髋置换髋臼前后壁偏心磨挫情况的差异。方法:纳入2015年10月至2017年12月在厦门大学附属福州第二医院由同一主刀医生行初次关节置换患者共60例,直接前侧入路组及后外侧入路组各30例(各占50%)。采用影像阅读系统收集并测量所有病例手术前后髋臼前壁、后壁横断面积,髋臼前后壁高、髋臼横径及髋臼前倾角的变化,以评价髋臼骨储量相关指标的变化。参加研究的人员均具有骨科手术5-10年的临床经验,手术医生职称为副主任医师以上。试验经厦门大学附属福州第二医院伦理委员会批准,批准时间2017-06-01。试验方案经患者和相关家属知情同意,并签署知情同意书。结果与结论:术后髋臼骨储量相关指标变化:两组术后前后壁横断面积、髋臼前后壁高度均小于术前(P0.05)。直接前侧入路组术后髋臼前壁面积高于后外侧入路组(P0.05)。结果证实:与后外侧入路行全髋置换相比,直接前侧入路术后髋臼前壁面积变大,其他髋臼前后壁骨储量参数指标变化不显著,试验推测直接前侧入路行全髋关节置换并不是导致髋臼前壁偏心磨挫的危险因素,未来还需多中心、前瞻性的随机对照研究来进一步验证。the Natural Science Foundation of Fujian Province,No.2017J01333(to FEY)~

The preparation and performance of alumina ultrafiltration membrane

由溶胶-凝胶法与水热法相结合,成功制备了氧化铝超滤膜。利用SEM、Xrd等表征手段,研究膜层晶型、粒子形貌、孔径大小及其分布、膜层厚度等的合理调控,并表征其通量、截留率和耐酸碱性能。结果表明,水热处理溶胶并于合适温度下烧结,可有效调控超滤膜膜层的晶型和粒子的形貌;通过分散剂PEg合适型号的选择,可有效调控膜孔孔径及其分布;合适的涂膜液浓度并适量的成膜助剂PVA,可有效调控膜层厚度并避免其开裂;当氧化铝呈α态时,超滤膜具有优秀的耐酸碱性能。Alumina ultrafiltration membrane was prepared by sol-gel hydrothermal method.SEM and XRD were employed to characterize the crystal form,particle morphology,pore size distribution and thickness of membrane layer surface so as to achieve a good control of the membrane layer characteristic.In addition,the flux, rejection,acid and alkaline resistances of the membrane were also tested.The results showed that crystal form and particles surface morphology of ultrafiltration membrane could be efficiently controlled by treatment with hydrothermal method followed by sintering at suitable temperature.Pore size and pore size distribution could be well tuned by using different types PEG as dispersant agents.The thickness and cracking of membrane layer could be efficiently controlled by changing the concentration of the coating solution and by adding suitable amount of PVA.It was found thatα-Al2 O3 ultrafiltration membrane displayed a better acid and alkaline resistance than that of transition-state

大连极紫外相干光源

先进光源的发展在前沿科学研究中发挥的作用越来越重要。近十年来,飞速发展的自由电子激光技术为科学家们提供了探索未知世界、发现新科学规律和实现技术变革的重要工具。建成的大连极紫外(EUV)相干光源的运行波段为50~150nm,单脉冲能量大于100μJ,且可提供10-12 s和10-13 s量级的超快激光脉冲,是我国第一台自由电子激光用户装置,并且是国际上唯一运行在极紫外波段的自由电子激光用户装置,在世界范围内为用户提供具有高峰值亮度和超短脉冲的极紫外激光。大连EUV相干光源是由国家自然科学基金委资助、由中国科学院大连化学物理研究所和上海应用物理研究所共同承担的重大科学仪器研制项目,目标是打造一个以先进极紫外光源为核心、主要用于能源基础科学研究的光子科学平台