Degradation performance of the digital coralline hydroxyapatite artificial bone scaffold in vitro

背景:前期实验成功制备了数字化珊瑚羟基磷灰石人工骨支架材料,并已证实其具有良好的理化性能和生物相容性。目的:评价数字化珊瑚羟基磷灰石人工骨的体外降解性能。方法:将珊瑚羟基磷灰石与左旋-聚乳酸分别以3∶1和4∶1的质量比混合,制备数字化珊瑚羟基磷灰石人工骨支架材料试件。将两种数字化人工骨支架材料、珊瑚羟基磷灰石及左旋-聚乳酸分别置于初始p H值为7.4的50 m L模拟体液中,在37℃恒温箱中降解16周,动态观察溶液p H值、钙及磷离子浓度,以及材料降解率、抗压强度及微观结构变化。结果与结论:降解16周时,两种数字化人工骨组的p H值维持在7.34-7.36,高于左旋-聚乳酸组(P3∶1数字化人工骨组>左旋-聚乳酸组>4∶1数字化人工骨组;两种数字化人工骨微孔结构增多,孔隙率升高,孔径增大,说明数字化珊瑚羟基磷灰石人工骨支架具有良好的降解性能。BACKGROUND: We have successfully prepared the digital coralline hydroxyapatite artificial bone scaffold in previous experiments, and it has good physicochemical properties and biocompatibility. OBJECTIVE: To evaluate the in vitro degradation performance of the digital coralline hydroxyapatite artificial bone. METHODS: We used the mixtures of coralline hydroxyapatite and L-polylactic acid at the mass ratio of 3:1 and 4:1 as raw materials to prepare the digital coralline hydroxyapatite artificial bone scafflold specimens, and then they were immersed in the 50 m L stimulated body fluid with the initial p H value of 7.4 in an incubator at 37 ℃ for degradation. After 16 weeks of degradation, the p H value, calcium and phosphate ion concentration, degradation rate, compressive strength and changes of microstructure were dynamically observed. RESULTS AND CONCLUSION: At the 16 th weeks of degradation, the p H values in the two kinds of digital artificial bone groups maintained at 7.34-7.36, which were higher than that in the L-polylactic acid group(P 3:1 digital artificial bone group > L-polylactic acid group > 4:1 digital artificial bone group. The cellular structure, porosity and pore size in the two kinds of digital artificial bone groups were all increased. These results show that the prepared digital coralline hydroxyapatite artificial bone scaffold has good degradation propertyies.福建省教育厅科技项目(JA12416);; 福建省自然科学基金青年创新项目(2013D013);; 漳州职业技术学院院级科研项目(ZZY1204)~

海水富营养化评价方法的研究进展与应用现状

简要概述了海水富营养化的概念及对生态系统的影响。介绍了几类常用的海水富营养化评价方法,包括单因子法、富营养化指数法、水质指数法和营养状态质量法等国内常用的评价方法,以及近年来国际上发展起来的以富营养化症状为考虑因素的第二代海水富营养评价方法,包括综合评价法、河口富营养化评价法和波罗的海行动计划等,第二代方法的评价标准更为细化,对富营养化带来的环境效应也提供一定的判断标准。阐述了现阶段我国海水富营养化评价方法的应用现状,尤其是在近海河口区域针对富营养化程度进行的区域等级划分,旨在提出各种海水富营养化评价方法应用现状和特点,为不同海域在选用富营养化评价方法提供参考。最后,对海水富营养化评价的应用方向提出未来展望,给出水质监测管理以及海洋生态环境灾害预测防御的指导性建议。国家重点研发计划海洋环境安全保障专项“中国近海与太平洋高分辨率生态环境数值预报系统”(编号:2016YFC1401605);;国家自然科学基金青年科学基金项目“南海低营养级生态模型营养盐循环过程的数值模拟研究”(编号:41206023)资助~

贺黄本立院士九十华诞

黄本立院士,1925年9月21日出生于香港,祖籍广东新会;光谱化学家,厦门大学教授。1945年—1949年就读于岭南大学。1950年—1986年任职于中国科学院长春应用化学研究所,历任助理研究员、副研究员、研究员;1986年调厦门大学任教至今。曾任中国化学会理事长、中国光谱学会副理事长、分析化学学科委员会主任委员、《光谱学与光谱分析》主编,《分析化学》、《分析科学学报》、《分析试验室》、《冶金分析》、《化学进展》等十多种国内期刊编委或顾问,SPECTrOCHIM

Study and develop on monitoring system database for precision manufacturing environment

针对精密加工中对环境参数控制的要求以及监控数据管理的需求,提出一种加工环境全局监控系统,研究其支撑的数据库开发。采用WIndOWS系统环境支持下的SQl SErVEr、VISuAl C++软件进行开发。系统承载数据实时监控、数据存储与淘汰、环境状况分析和及时报警等模块功能,提高了诊断的准确性,并针对精密非球面磨削数控加工,设计了监控系统实例。最后,通过系统测试验证了其可行。This paper presents a monitoring and whole situation control system for machining process according to the characteristic of precision manufacturing,and studies on its system database development.In this paper,the database was established by adopting Visual C++ and SQL Server supported based on Windows system.There are real time monitoring,data storage and elimination,environment conditions analysis,and timely alarm modules in this system,for improving the data management and accuracy of the system.Finally,an example of monitoring system for precision aspheric surface manufacturing was designed and verified by test.国家自然科学基金项目(51075343

Solid-State Electrochemiluminescence on Tris(2,2′-bipyridine) ruthenium(Ⅱ) Modified Electrode

由于电致化学发光(ECL)的连续可测性、高灵敏度、稳定和方法简单,被广泛应用于分子生物学、药学、化学和环保等领域。而将可电化学再生的ECL试剂固定于电极表面,可获得ECL传感器,从而减少分析过程中试剂的消耗并简化实验装置。联吡啶钌及其衍生物在电极表面的固定化研究成为ECL研究的重要方向。本文综述了2004年以来联吡啶钌及其衍生物修饰电极的固相ECL的研究进展,并简要介绍了各种固定方法及其相关的应用情况。Electrochemiluminescence(ECL) has been widely used in biological,pharmic,chemical and environmental applications due to its continuance,sensitivity,reproducibility and relative easy to control.The ECL sensor can be assembled by immobilizing electrochemically reproducible ECL reagents on an electrode surface.This approach will reduce the reagents consuming and simplify the detection apparatus.Recently,the studies on the immobilization of tris(2,2′-bipyridine)ruthenium(Ⅱ) [Ru(bpy)_3~(2+)] and its derivations have becoming an attractive research field.This review summaries the solid-state ECL development of Ru(bpy)_3~(2+) and its derivations since 2004.The approaches for reagent immobilizations as well as the related applications are also discussed.教育部新世纪人才奖励计划;; 福建省科技项目(No.2005-I-030)资

Effects of Jiangu Granules on the proliferation of osteoblasts through G_1/S phase cell-cycle regulated proteins

目的观察健骨颗粒对成骨细胞G_1/S期调控的影响,探讨健骨颗粒促进成骨细胞增殖的作用机制。方法制备健骨颗粒血清组、模型血清组和雌二醇血清组。采用酶消化法培养SD大鼠成骨细胞,健骨颗粒含药血清干预,以模型血清和雌二醇血清为对照。运用流式细胞术检测成骨细胞增殖周期,荧光定量PCR法检测成骨细胞G_1/S期调控蛋白Cyclin E、CDK2、p21和转录因子E2F-1 m RNA的表达。结果 15%的雌二醇血清与健骨颗粒血清干预48 h,成骨细胞增殖速度均明显快于模型血清组(P<0.01);G_0/G_1期成骨细胞比例明显降低(P<0.01),S期、G_2/M期细胞比例及增殖指数则明显高于模型血清组(P<0.01);与模型血清组比较,雌二醇血清组与健骨颗粒血清组能提高成骨细胞Cyclin E、CDK2及转录因子E2F-1 m RNA的表达(P<0.01),而降低p21的表达(P<0.01)。结论健骨颗粒通过调节成骨细胞G_1/S期调控机制,推进成骨细胞顺利通过G_1/S检测点,促进成骨细胞增殖。Objective To observe the effects of Jiangu Granules on the G_1/S phase cell-cycle regulated proteins of osteoblasts, so as to reveal the mechanism of Jiangu Granules in promoting osteoblasts proliferation. Methods The Jiangu Granules-serum group, model-serum group and estradiol-serum group were prepared. Osteoblasts of SD rats were cultivated by enzymatic digestion and intervened with Jiangu Granules-serum, model-serum and estradiol-serum(as control) respectively. The cell cycles were analyzed by flow cytometry, while the fluorescence quantitative PCR was applied to measure cyclin E, CDK2, p21 and E2F-1 m RNA. Results The cell proliferation rates of osteoblasts intervened with estradiol serum and Jiangu Granules-serum for 48 hours were faster than that with the same concentration model-serum(P < 0.01). Compared with the model-serum group, the proportion of osteoblasts in the G_0/G_1 phase were significantly reduced after intervention with 15% of estradiol serum and Jiangu Granules-serum for 48 hours(P < 0.01),while the proportion of cells in S phase, G_2/M phase and proliferation index was much higher(P < 0.01). The expression of cycling E, CDK 2 and transcription factor E2F-1 m RNA of osteoblasts in the Jiangu Granules-serum group and the estradiol-serum group was higher than that of model-serum group(P < 0.01), while the expression of p21 m RNA was lower than the model-serum group(P < 0.01). Conclusion Jiangu Granules-serum can adjust the G_1/S phase cellcycle regulated mechanism in osteoblast, so as to push the cells passing G_1/S checkpoint and accelerate the proliferation of osteoblast.国家自然科学基金资助项目(81473706

交联型聚芳醚基阴离子交换膜的制备及性能研究

燃料电池由于能量转化率高、环境友好等优点,是最具应用前景的能量转化装置之一。阴离子交换膜作为燃料电池的核心部件,仍存在低电导、高溶胀等问题.这里,通过设计聚芳醚结构使其侧链末端含有碳碳双键,在Grubbs二代催化剂作用下进行烯烃复分解反应接枝离子基团,利用剩余不饱和双键在高温下的交联反应,制备出交联型聚芳醚基阴离子交换膜.由于亲水性侧链与疏水性主链的不兼容性,使膜内形成了有利于离子传输的微相分离结构,交联膜的最高电导率达到81.1mS/cm(80℃).此外,交联结构能有效地抑制膜的溶胀,交联膜C-FPAE-PH-1.5在30℃下的溶胀率仅为7.51%,表现出优异尺寸稳定性.C-FPAE-PH-1.5膜在60℃下1mol/L KOH水溶液中浸泡360h后,保留了93.1%的电导率,表现出优异的耐碱性能.国家自然科学基金(21576226

Effect of Exogenous Nitric Oxide Donor Sodium Nitroprusside on Stomatal Movement in Leaves of Mangrove Plant Aegiceras corniculatum

探索一种用材简单、操作方便、真实性强的观察红树植物桐花树叶片气孔的制片技术,并利用该技术研究不同浓度、不同处理时间的一氧化氮(nO)供体硝普钠(SOdIuM nITrOPruSSIdE,SnP)对桐花树气孔开闭的影响,探讨了nO调控的气孔运动与外源CA2+的关系以及nO与H2O2在调节气孔运动过程中的关系。结果表明:在搅碎法、指甲油印迹法、牛皮胶印迹法三种观察气孔方法中,牛皮胶印迹法是观察气孔开度变化的最佳方法。nO能够诱导桐花树气孔快速关闭,且表现出明显的时间效应与浓度效应。nO导致的气孔关闭与CA2+的参与有密切关系,nO与H2O2存在明显的协同效应,可以促进气孔关闭。The aim of the present paper was to find out a simple and easy-to-go method to observe the stomatal movement of a mangrove plant Aegiceras corniculatum.The new method was used to study the following areas, inculing the effect of exogenous nitric oxide (NO) on the leaf stomatal movement under different concentrations and different treated time, the effect of Ca2+ in the regulation of stomatal movement induced by exogenous NO, the interactional effect between NO and H2O2 in the regulationb of stomatal movement.The results suggested that the oxhide pastern print was the best way to observe stomatal movement of A.corniculatum compared with the blender method and the nail enamel print method.The stomatal closure induced by NO was associated with Ca2+ in dose dependent manner.It was also proved that a rapid stomatal closure could be co-induced by NO and H2O2 in A.corniculatum guard cells.国家自然科学基金项目(30770192;30670317);厦门大学“新世纪优秀人才支持计划”项目(X07115);湖北民族学院博士启动基金项

哌啶阳离子功能化侧链型阴离子交换膜的制备

燃料电池因其能量转化率高、污染小等特点,成为当前的研究热点.然而,作为关键部件的阴离子交换膜仍然存在离子电导率低、耐碱性能差等缺点,这限制了燃料电池的发展.利用威廉姆森成醚反应在聚醚酮主链接枝哌啶鎓盐,成功制备了侧链型阴离子交换膜.设计的哌啶鎓盐通过长柔性亚甲基与主链相连,使膜内形成有利于高效离子传输的微相分离结构,PEKCQA-1.0膜在80℃的电导率高达72.7mS/cm.此外,哌啶阳离子基团离主链较远,减弱了对主链的吸电子作用;同时,环型结构的哌啶阳离子具有一定的空间位阻,减少了OH~-对阳离子基团的攻击,使膜表现出优异的耐碱性能.PEK-CQA-0.8膜在60℃下1mol/L KOH水溶液中浸泡360h后,离子电导率仅下降了8.8%,有望应用于碱性燃料电池.国家自然科学基金面上项目(21376194,21576226

新型环三藜芦烃衍生物的合成及其热致液晶行为的研究

设计了先关环再衍生的方法,合成了系列外围含不同反应性官能团的新的环三藜芦烃(CTV)衍生物.利用1 H-NMR、13C-NMR、元素分析、质谱等对合成的CTV衍生物进行了结构表征.研究表明,分子间作用力增大会导致CTV衍生物的热致液晶性减弱,结晶性增强.合成的CTV-A具有热致液晶性;CTV-D和CTV-E由于氨基的存在产生了较强的分子间氢键作用,既形成了热致液晶态,又发生冷结晶;而CTV-B和CTV-C由于含有羧基、羰基、羟基或氨基等,分子间作用力强,只出现冷结晶,不能形成热致液晶.研究发现,通过外围基团的分子设计,调节氢键能力或柔性间隔基,可调节分子间作用力和熵的大小,从而控制CTV衍生物的热致液晶性