深化高校职员制度改革促进职务与职级的和谐统一

文章介绍了厦门大学试行职员制度的聘用原则、聘用条件、主要特点和试行的意义与成效,并提出了深化高校职员制度改革的思考与建议,可供其他高校参考

化学模拟生物固氮——Ⅸ 铁钼辅基模型化合物的合成和性能表征

本文曾在全国化学摸拟生物固氮协作组领导小组扩大会议(昆明,1980,8)上宣读。[中文文摘]采用乙二醇基阴离子作为活插头(可通过水解除去的双配位螯形配位体),对前文提出的合成方法作了重要改进,以期所合成的铁钼辅基模型化合物中Mo~(Ⅳ(Ⅲ))第一配位界内两个不稳定的配位体处于相邻的位置,如厦门模型Ⅲ(或厦门模型Ⅱ)所要求。合成和重组活性评价结果,重组活性比使用乙腈等为活插头的提高2个数量级,化学催化活性和选择性接近于天然FeMo-co水平。[英文文摘]A significant improvement has been made to the method previously proposed[3] for synthesizing FeMo-co modeling compounds. With the use of ethylene glycolate anion as labilizable blocking agent (hydrolyzable bidentatc chelating ligand)to protect two neighboring coordination sites in the first coordination sphere of MoIV(III) of the synthesized FeMo-co modeling compound, an increase in reconstituted-nitrogenase activity of 2 orders of magnitude of the sample synthesized (as compared with the use of acetoni-trile or other monodentare ligands as bloeking agents)has been obtained.Catalytic activity and selectivity assays as well as preliminary characterization by EPR method have also been made

ECHS1 Involved in Antagonising Apoptosis in HepG2 Cells via the Mitochondrial Pathway

目的:观察烯脂酰辅酶A水合酶短链1(EnOyl-COA HydrATASE SHOrT CHAIn 1,ECHS1)对HEPg2细胞凋亡的影响。方法:构建ECHS1基因的干扰质粒,转染HEPg2细胞后通过嘌呤霉素筛选稳定干扰ECHS1的细胞株(HEPg2-SIECHS1),利用WESTErn blOT验证其干扰效率;利用流式细胞仪及TunEl方法检测ECHS1干扰后HEPg2细胞凋亡情况,再采用WESTErn blOT检测凋亡相关蛋白表达水平。结果:成功构建了ECHS1的干扰质粒;WESTErn blOT验证了ECHS1干扰后HEPg2细胞株中ECHS1表达低于空白对照组(未转染HEPg2细胞)及阴性对照组(空载体Pu6转染HEPg2细胞)(P<0.05);流式细胞仪术显示HEPg2-SIECHS1组细胞株凋亡率(14.98±1.07)%,阴性对照组(10.55±0.19)%,两者差异有统计学意义(P<0.05);TunEl实验显示HEPg2-SIECHS1组细胞株凋亡率(6.13±0.12)%,阴性对照组(2.89±0.21)%,两者差异有统计学意义(P<0.05);与阴性对照组比较,ECHS1干扰后P53、促凋亡蛋白bAX及bId均表达增加。结论:ECHS1通过线粒体途径拮抗HEPg2细胞凋亡。Objective: To investigate the impact of enoyl-CoA hydratase short chain 1(ECHS1) on apoptosis of HepG2 cells.Methods: The ECHS1 interference plasmids were constructed and transfected into HepG2 cells.The stable interference cell lines were screened via puromycin.Interference efficacy was detected by Western blot.Flow cytometry and TUNEL assays studied the apoptosis of HepG2 cells.Apoptosis-related proteins were detected by Western blot.Results: The HepG2 cells with stable ECHS1 gene interference were successfully established.The expression level of ECHS1 protein in HepG2 cells after transfection with ECHS1 siRNA was significantly lower than that in the blank control cells(HepG2 cells without transfection) and the negative control cells(HepG2 cells transfected with pU6 vector)(P< 0.05).Apoptosis ratio of the ECHS1 siRNA group was significant higher than that of negative control group by both Flow cytometry and TUNEL assays(P<0.05).Expressions of p53,Bax and Bid in ECHS1 siRNA group were higher than those in negative control group.Conclusion: ECHS1 may antagonise the apoptosis of HepG2 cells via the mitochondrial pathway.南京军区医学科技创新课题(10MA070); 厦门市科技计划医疗卫生创新项目(3502Z20124029)资助项