Preliminary Study of Neutralizing Mimotope Vaccine for High Pathogenic Avian Influenza Virus H5N1

禽流感，是目前威胁人类健康与生命的急性传染病。禽流感在人类间的传播已引起广泛的国际关注，专家预言人类正处于全球流感大流行的危险关口。由于许多流感病毒甚至具有由人传人的能力，其所造成的危害将是灾难性的。近年来由高致病性流感毒株H5N1引起的遍及东南亚十个国家的流感暴发尤为严重，对禽流感的有效控制已刻不容缓。本文对禽流感相关疫苗的开发情况进行了简要的回顾，并着眼于具有巨大发展潜力的新型疫苗－表位疫苗的研制。首先，运用目前抗原表位研究最有力的工具之一－噬菌体肽库技术开展本研究。基于本实验室在禽流感单克隆抗体筛选与制备方面的优势，以流感病毒HA基因中和性单克隆抗体8H5为靶分子，对噬菌体随机环7肽库进...Avian Influenza, the new threat against we human beings, has been on the stage over the past few years and still yet to give their ways. Cases of human infection have raised international concern that we could be on the brink of a global influenza pandemic. Since many of these infections have been able to transmit efficiently from person to person, the effect would have been devastating. The outbr...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：20032606

Advances for the Ruthenium Complexes-Based Homogeneous Catalytic Hydrogenation of Oxalates to Ethylene Glycol

草酸酯加氢是重要的有机化学反应,在工业制乙二醇生产中有着重要的应用.对钌金属配合物均相催化反应的研究进行了综述.以催化反应体系为焦点,探讨了多种; 因素如温度、氢气压力、催化剂浓度、反应时间、添加剂等对底物转化率以及产物选择性的影响,并讨论了催化反应机理,其中金属-配体协同促进的H_2分子异; 裂,进而完成对底物分子中酯基依次加氢的反应机理是探讨的重点,这为新型催化剂的设计和应用提供参考.Hydrogenation of oxalates is one of the important organic reactions, which has an ultimate use for the industrial production of ethylene glycol. The studies on the ruthenium complexes-based homogeneous catalytic reaction systems are herein summarized. With the focus on the catalytic reaction systems, the important factors with significant influences on the oxalate transformation efficiency as well as the product selectivity are discussed, including temperature, H-2 pressure, catalyst concentration, reaction duration, additives, and so on. The catalytic reaction mechanisms are also discussed in detail, where the mechanism for the H-2-heterolysis promoted under the metal-ligand cooperation for the oxalate hydrogenation to ethylene glycol is enhanced. This study would be useful for designing the new catalyst applicable in industry.National Natural Science Foundation of China [21473142, 91545115,; 21473145]; Innovative Research Team of China [IRT_14R31, J1310024

人肝细胞内戊型肝炎病毒结合蛋白的酵母双杂交筛选

为进一步深入研究戊型肝炎病毒(HEV)感染机制以及致病机理,用酵母双杂交系统从人肝细胞cDNA文库中筛选与戊型肝炎病毒衣壳蛋白E2相互作用的蛋白,核酸序列分析及同源性检索表明,4个克隆与E2相互作用,其中一个克隆与P38IP高度同源,细胞免疫共沉淀实验结果显示:在哺乳动物细胞水平仍能够检测到E2与P38IP片段的特异的相互作用

超声速压缩-膨胀构型激波与湍流边界层干扰直接数值模拟研究

采用直接数值模拟方法对来流马赫数2.924&deg;压缩-膨胀构型中的激波与湍流边界层干扰问题进行了数值研究。系统探究了膨胀角法向高度对干扰区内的若干基本流动现象的影响,如分离泡、激波非定常运动、湍流边界层的演化特性等。研究发现,随着膨胀角法向高度减小,膨胀效应导致分离泡流向与法向尺度均急剧减小。物面压力脉动信号预乘谱结果表明,膨胀效应抑制了分离激波的低频振荡运动。相较于压缩折角构型,压缩-膨胀构型下游存在复杂的膨胀波系结构,导致下游湍流边界层的内层、外层出现相反的恢复过程。</p

Decomposition of mean skin friction in incident shock wave/turbulent boundary layer interaction flows at Mach 2.25

The evolution characteristics of the mean skin friction beneath the supersonic turbulent boundary layer that interacts with incident shock waves at Mach 2.25 are analyzed using Direct Numerical Simulation (DNS). The separated and attached boundary layers in the interaction region that respectively correspond to 33.2 degrees and 28 degrees incident shock angles are considered. The mean skin friction recovery rate for the separated boundary layer is much gentler and distinctly less than that for the attached case where the skin friction completes its recovery within one boundary layer thick-ness. The novel mean skin friction decomposition method for compressible flows proposed by the recent research is applied in the interaction region to investigate the internal evolution characteristics quantitatively. The results reveal that the three decomposition components are distinctly unequal between the two cases. The contributions of the turbulent motions at different scales to the associated term are focused on using empirical mode decomposition technology. It indicates that the outer large-scale structures dominate separation and reattachment regions, while contributions from inner small-scale structures are limited. In contrast, contributions from the outer large-scale structures are dramatically reduced in the attached case, which results in the outer large-scale and inner small-scale motions being of equal importance.(c) 2023 Production and hosting by Elsevier Ltd. on behalf of Chinese Society of Aeronautics and Astronautics. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/)

压缩-膨胀湍流边界层平均摩阻分解

采用直接数值模拟对来流马赫数2.9、24°压缩-膨胀折角构型中激波与湍流边界层干扰问题进行了研究。重点关注膨胀折角法向高度对激波干扰区以及下游平板边界层流动的影响。研究发现,当高度足够大时,激波干扰区内未受下游膨胀波的影响,此时的流动特征与传统的压缩折角干扰构型一致。高度较小时,脱体剪切层的再附过程受到下游膨胀波的加速影响,导致再附点向上游移动,分离泡发生剧烈收缩。对上、下游平板湍流边界层应用了平均摩阻分解技术,比较了湍流边界层在平衡和非平衡状态下的差异。分析发现,膨胀折角区域的高摩阻现象主要与摩阻分解后的C_(f1)项与C_(f3)项相关。高度变化对C_(f1)项影响较小,而对C_(f2)项影响显著。高度变化体现在:下游平板上G9rtler涡结构强度以及层流化现象对C_(f2)项贡献的差异