72 research outputs found
Determination for the Contents of Neu5Ac, Neu5Gc and KDN in Organ Meats and Muscle of Pigs by LC-MS/MS
目的:建立液相色谱-三重四级杆质谱联用法(LC-MS/MS)测定猪内脏和肌肉组织中唾液酸N-乙酰神经氨酸(Neu5Ac)、N-羟乙酰神经氨酸(Neu5Gc)和脱氨神经氨酸(KDN)浓度的分析方法。方法:将内脏肉组织匀浆后,利用三氟乙酸(TFA)将结合态的唾液酸从糖脂或糖蛋白链上解离下来后进样分析。色谱条件:色谱柱为ZORBAX Eclipse Plus C18(5μm,4.6×250 mm,Waters),流动相为0.1%醋酸铵-100%乙腈(9:1),柱温:25℃,流速800μL·min~(-1),进样量20μL。质谱条件:离子源为电喷雾化离子源(ESI),扫描方式为多重反应监测(MRM),监测离子对:Neu5Ac:307.7→87.0,Neu5Gc:323.3→116.0,KDN:266.7→87.0,13C3Neu5Ac内标品:310.9→90.0。结果:Neu5Ac、Neu5Gc和KDN分别在0.1~20.0、0.05~10.00和0.005~1.000μmol·L~(-1)的浓度范围内和对照品与内标峰面积比值线性关系良好,重复性平均RSD为1.2%,稳定性平均RSD为1.9%,日间和日内精密度试验RSD均小于6.7%,平均回收率为92.9~106.4%。结论:本方法简便、快速、灵敏度高,可广泛运用于组织和体液中唾液酸的测定。Objective: To establish an LC-MS/MS method for the detection of N-acetylneuraminic acid(Neu5Ac),N-glycolylneuraminic acid(Neu5Gc) and 2-keto-3-deoxy-D-glycero-D-galactonononic acid(KDN) in organ meats and muscle of pigs.Methods: Tissues were acid hydrolyzed with trifluoroacetic acid(TFA) to get ganglioside-bound and glycoprotein-bound sialic acid,followed by injected and analyzed. The analysis was performed on an ZORBAX Eclipse Plus C18(5 μm, 4.6 ×250 mm, Waters)column, and the mobile phase consisted of 0.1 % ammonium~-100 % acetonitrile(9:1), the flow rate was 800 μL/min, the temperature was maintained at 25 ℃, injection volume was set up 20 μL. Negative electrospray ionization(ESI) and multiple reaction monitoring(MRM) mode was used, MRM: Neu5Ac: 307.7→87.0, Neu5Gc: 323.3 →116.0, KDN: 266.7→87.0, 13C3Neu5Ac(IS): 310.9→90.0.Results: The ratio of internal with standard sample peak area and content of Neu5 Ac, Neu5 Gc and KDN showed good linearity in the range of 0.1~20.0, 0.05~10.00, and 0.005~1.000 μmol/L, RSDs of the repeatability and stability were 1.2 % and 1.9 %, Intra-and inter-day RSDs were all lower than 6.7%, the recoveries of sialic acid were 92.9~106.4%. Conclusion: The established method is simple,accurate and rapid, higher sensitivity and can be applied to determination of Neu5 Gc, Neu5 Ac and KDN in tissues and fluid of animals and human.国家自然科学基金项目(31271038
岩体微平面损伤模型的Abaqus用户子程序Vumat验证
本文探讨岩体微平面损伤模型~([1])在Abaqus中的实现。对课题组编制的Vumat用户子程序,采用砂岩三轴压缩试验进行算例验证。算例分析表明,该模型能表征砂岩的脆延转换宏观力学行为对应的各向异性损伤力学机制
企业集团对创新产出的影响:来自制造业上市公司的经验证据
基于2003—2015年中国制造业上市公司的研究样本和手工收集的企业集团信息,本文系统检验了企业集团如何影响企业的创新产出及其影响机制。研究表明,企业集团与专利产出之间存在着显著的正向关系,并且这一关系对发明专利更为显著。基于2003年国务院国有资产监督管理委员会成立后各地区推出"企业集团促进政策"改革构建工具变量进行检验,上述发现仍然显著成立。进一步研究发现,从供给侧看,企业集团通过内部资本市场缓解了创新的"融资约束"难题,通过内部知识市场缓解了创新的"信息匮乏"难题;从需求侧看,由于专利产出可以在各成员间灵活地配置和使用,因此,企业集团创新产出的市场回报率更高。本文的研究不仅丰富了企业组织形式与创新产出的相关文献,也对推进国有经济战略布局调整、支撑创新驱动发展提供了有益启示。国家自然科学基金青年项目“企业集团、货币政策与现金持有水平”(批准号71402005);;广东省普通高校人文社会科学研究项目“互联网金融模式及其风险监管研究”(批准号2014GWXM017);;广东省哲学社会科学“十三五”规划项目“企业专利质量评估及其价值相关性研究”(批准号GD16YYJ03
The Research on the Relationship between the College Students’ Parenting Style and Self-Confidence
Recent progress in flexibility effects on wing aerodynamics and acoustics
Since the theoretical aeroelasticity for flapping-wing aerodynamics was introduced in the 1920s, the effects of flexibility on aeroelasticity have been paid more attention to aerodynamic design. In recent years, the trait of the wing flexibility is applied for small-scale wings of biomimetic flyers including micro air vehicles and mini unmanned aerial vehicles. Until now, the aerodynamic performance and great agility of these flyers, which are beneficially used for military missions and other civilian applications, have been improved through surrogate flapping wings with the favorable effects of the flexibility. As per the aeroelasticity principle for the forward flying, the chordwise flexibility of an elastic flapping wing can generate thrust and lift redistributions, whereas the spanwise flexibility can result in variations of the angle of attack and the shift of phase along the wingspan direction. Consequently, all vortices generated by the flapping wing i.e. (1) leading-edge vortices, (2) tip vortices, and (3) trailing-edge vortices are blended supportively, thereby improving the aerodynamic performance and agility. Hence, the growth of research and development of the aerodynamic performance and agility for these flyers under the influence of flexible wings increases through experimental and computational studies dynamically and rapidly. This review aims to highlight the important role of the flexibility in the recent progress in wing aerodynamics of these flyers through several wing models done by famous groups of experts in this field. In addition, this review includes the acoustics of the wings under the flexibility effects which is considered as a new key for better flyer design and improvement. A comprehensive understanding of the integrated aerodynamics and acoustics under the wing flexibility is, therefore, needed
退火温度对金属催化四面体非晶碳转变为石墨烯过程的影响
设计了金属催化剂Ni/四面体非晶碳(ta-C)/基底三层结构,使用磁过滤阴极真空电弧设备制备了ta-C薄膜,用电子束蒸镀技术制备Ni薄膜,并对其进行快速热处理调控非晶碳转变石墨烯的过程,重点研究了热处理温度对石墨烯生长的影响。结果表明,沉积态的ta-C和Ni层均表面平整、均匀致密,其中Ni薄膜呈(111)晶面择优取向生长,为石墨烯的高质量生长提供了条件。同时,退火温度显著影响了非晶碳的石墨烯转变,当退火温度高于400℃时Ni表面能生成多层石墨烯,在500℃保温15 min可制备出质量较高的多层石墨烯
Analysis of NPP driving force in an arid region of Northwest China: A case study in Yili Valley and parts of Tianshan Mountains, Xinjiang, China
Application of smell identification test in Parkinson's disease in China
目的 建立我国嗅觉识别测验材料与方法,测查帕金森病患者的嗅觉功能,验证测验的有效性,分析其影响因素。方法 选取诊断明确的原发性非痴呆帕金森病患者55例以及与其人口学资料相匹配的健康对照55名作为研究对象,采用中国科学院心理研究所研发的基于中国人群的嗅觉识别测验方法,进行嗅觉识别测验。结果 帕金森病组嗅觉识别测验的正确选择数(19.3±5.4)显著少于健康对照组(26.5±5.7),差异有统计学意义(t=-6.827,P〈0.01);依据不同年龄段的参考值进行分段比较,发现63.6%的帕金森病患者存在显著嗅觉减退,Hoehn-Yahr分级2~3级显著嗅觉减退的发生率为77.8%;通过帕金森病组与健康对照组受试者工作特征(ROC)曲线分析显示,嗅觉识别测验正确识别数取界值为22.5时,诊断嗅觉减退的敏感度为74.6%,特异度为70.9%;帕金森病患者嗅觉识别测验正确数不受年龄、教育年限、Hoehn-Yahr分级、病程、统一帕金森病评定量表的第三部分得分、MMSE评分的影响(均P〉0.05)。结论 帕金森病患者存在嗅觉识别能力的减退,中国人群的嗅觉识别测验能够有效地测查帕金森病患者的嗅觉功能。</p
羊草种子萌发相关基因的筛选及表达分析
前期实验表明,变温处理(28℃12h/16℃12h)可显著提高羊草种子的萌发率,且羊草种子萌发中的第1天是接受变温信号的关键时期。以此为研究基础,结合羊草种子变温萌发的转录组测序数据,针对羊草种子萌发初期对变温处理的响应筛选出与种子萌发、休眠及低温相关的基因24个,利用测序结果中这些基因的RPKM值制作基因表达热图并分析其表达差异。以萌发率高、低的两种羊草种质的种子为材料,对24个基因在恒温12h(28℃)和变温1d(28℃12h/16℃12h)萌发处理中的表达分别进行了定量分析。结果表明,与恒温对照相比,变温处理12h后,表达明显上调的基因有SAIN1,PP2C62,EXPB3,EXPB4,GA3ox,EXPA2和EXPA7,而表达明显下调的基因有bHLH49,GID1,ABI8,Chi1,11833,CBF3,NAC2,PP2C72,SAIN2和5423。通过进一步分析相关基因在高、低萌发率两个种质中表达的差异,筛选出其中可能与羊草种子萌发相关的基因有几丁质酶基因Chi1,转录因子基因CBF3,羊草新基因5423,赤霉素合成基因GA3ox,细胞松弛素蛋白基因EXPB4和羊草新基因SAIN1,将为下一步阐明羊草种子萌发的分子作用机理奠定基础
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