SPECIATION ANALYSIS OF TRACE ELEMENTS IN GANODERMA ATRUM

研究黑灵芝中微量元素含量及溶出特性对黑灵芝质量控制、灵芝产品的合理开发利用有重要作用。用ICP-MS法同时测定黑灵芝中十余种微量元素的初级形态含量及分布,考察了黑灵芝中微量元素在不同极性溶剂中的浸出率,并通过国家一级标准物质验证了分析结果的准确性。结果表明,随着溶剂极性增大,大部分元素的溶出率增大。水提取黑灵芝,铜、锰、铁、锌等元素的提取率均小于40%,对黑灵芝样品进行了加标回收试验,回收率在88.2%~110.6%。It is important to study the trace element contents and transference characteristics and element speciation in the extract of Ganoderma atrum for the quality control and reasonable exploitation. In this experiment the contents and speciation of more than ten elements in Ganoderma atrum were simultaneously determined with microwave assisted digestion of sample combined with inductively coupled plasma mass-spectrometry (ICP-MS). The dissolution percentage of trace element in different solvents were also studied and the veracity of the analytical results were validated with CRMs′ under the same conditions.The results indicated that the dissolution percentage of most elements in solvents were increased with its polarities. In the aqueous solution of the Ganoderma atrum, the extraction percentage of Cu, Mn, Fe, Zn were all lower than 40%. The recoveries of the elements were ranged from 88.2% to 110.6%

Flame Characteristics and Burning Rate of Typical Solid Fuel Under Reduced Gravity

利用实验时间为2.2 s，低重力水平为l0-2 90简易实验系统，对易升华典型固体燃料试样从常重力到低重力环境过渡的瞬态燃烧过程进行了实验研究，考察了火焰形态、火焰亮度和温度以及燃料试样燃烧速率受重力水平影响的变化趋势，结果表明，随着重力水平的变化，火焰从常重力环境中的湍流扩散火焰，过渡为低重力环境中的层流火焰，火焰高度、亮度和温度以及燃烧速率都随重力水平的下降而降低．由于进入低重力状态后，常重力燃烧诱导的空气流动并没有完全消失，观察到了逆风侧火焰温度、火焰平均亮度，以及燃烧速率因气流作用而回升的现象，这种现象会使低重力环境中的火灾复杂性和危害性增强

Measuring trace elements in alisma orientalis (Sam) juzep from four districts with ICP-MS

本文采用电热板—HNO_3—H_2O_2消解体系,电感耦合等离子体质谱(ICP—MS)法对分别产于福建建瓯、福建龙海、江西彭山、四川罐县等四个不同地区的泽泻进行微量元素的定量分析,通过数据处理统计,对泽泻中微量元素含量测定的结果、方法的准确性及其应用进行初步探讨。TCP - MS analytical technique has been used to measure the content of multi-elements in Alisma orientalis (Sam). Juzep, samples with the electrothermal plate and HNO3 - H2O2 dissolved system. The samples come from four districts: Jian'ou of Fujian province, Longhai of Fujian province, Pengshan of Jianxi province and Guanxian of Sichuan province. According to the data processing, we discuss the different content of trace elements from the selective four districts. evaluate the accuracy of this mthod and debate the application primarily.国家自然科学基金;; 福建省中药质量控制重点项

Photosynthetic capacity of three common species of macroalgae and the application in coral aquarium

利用水下饱和脉冲调制叶绿素荧光仪(dIVIng-PAM)测定了珊瑚养殖水处理中3种常见大型海藻石莼(ulVA lACTuCA)、条浒苔(EnTErOMOrPHA ClATHrATA)和羽状蕨藻(CAulErPA MEXICAnA)光能转化效率(fV/fM),快速光曲线(rlC)相关参数和非光化学淬灭(nPQ)。结果表明:石莼和条浒苔的fV/fM分别为0.808±0.004和0.816±0.009,显著高于羽状蕨藻。最大相对电子传递速率rETrMAX(17.52±2.92和19.59±4.43μMOl E·M-2·S-1)、半饱和光强Ik(53.41±8.18和59.71±13.52μMOl PHOTOnS·M-2·S-1)也明显高于羽状蕨藻(rETrMAX和Ik分别为13.72±5.41μMOl E·M-2·S-1和32.67±14.06μMOl PHOTOnS·M-2·S-1),表明石莼和条浒苔有着比羽状蕨藻更高的光合能力和对强光的耐受能力,光保护能力更强;羽状蕨藻较高的rlC初始斜率α表明其在弱光下拥有更强的光捕获能力,弱光下光合效率更高;随光照的增强(0~373μMOl PHOTOnS·M-2·S-1),羽状蕨藻nPQ的增幅有限(0~0.2),最大值仅为条浒苔和石莼的38.1%和62.5%,表明这种藻类更容易受到强光的抑制。因此,在珊瑚养殖的水处理中,可以根据不同的光照选择适宜的藻种或藻种组合并根据光梯度进行布局,反之也可以根据藻种来选择合适的光源,以达到最佳的处理效率。This study investigated the difference of photosynthetic capacity in three macroalgae species( Ulva lactuca,Enteromorpha clathrata,and Caulerpa mexicana) commonly used for nutrient removal in coral aquariums.The photochemical efficiency( Fv/Fm),parameters relevant to rapid light curve( RLC) and non-photochemical quenching( NPQ) in the macroalgae were measured by underwater saturation pulse modulated chlorophyll fluorometer( Diving-PAM).The results showed that Fv/Fmwas 0.808 ± 0.004 for U.lactuca and 0.816 ± 0.009 for E.clathrata,which were higher than that for C.mexicana.Higher maximum relative electron transfer rates( rETRmax)( 17.52 ±2.92 and 19.59 ±4.43 μmol e·m- 2·s- 1) and half-starved value of light intensities( Ik)( 53.41 ±8.18 and 59.71 ±13.52 μmol photons·m- 2·s- 1) were observed in U.lactuca and E.clathrata than that in C.mexicana( 13.72 ± 5.41 μmol e·m- 2·s- 1for rETRmaxand 32.67 ± 14.06 μmol photons·m- 2·s- 1for Ik),indicating that U.lactuca and E.clathrata have stronger photosynthetic capacities,photo-protection capacities and tolerance to higher intense light than C.mexicana.A significantly lower RLC initial slope α in C.mexicana implied that this algal species has a higher photosynthetic capacity under weak light condition.With increasing the light intensity( 0- 373 μmol photons·m- 2·s- 1),however,C.mexicana had a limited increase of NPQ( 0- 0.2),the maximum of which was equivalent to 38.1% of that in U.lactuca and 62.5% of that in E.clathrata.These results indicate that the photosynthesis of E.clathrata may be more easily limited by intense light.Our study suggests that the selection of algal species for nutrient removal in a coral aquarium should be adapted to the light intensity.Single species or the combination of different species of macroalgae can be selected according to the illumination of the coral aquarium in order to maximize the efficiency of nutrient removal.国家海洋局第三海洋研究所基本科研业务项目(海三科2011033); 厦门海洋研究开发院共建项目; 海洋公益性行业科研项目(201105012)资

Study on the Elements and Element Speciation in the Extract of Taizishen

目的对太子参水提液中3种金属元素Cu,Mn,Zn进行形态研究。方法采用电感耦合等离子体质谱(ICP-MS)测定太子参及太子参水提液中3种金属元素的含量,并用反相高效色谱(RP-HPLC)-电感耦合等离子体质谱(ICP-MS)联用技术对太子参水提液中的Cu,Mn,Zn元素进行形态分析。结果太子参水提液中金属元素Cu,Mn,Zn含量较高,其中Cu,Mn,Zn均主要以结合态形式存在。结论该实验所建立的HPLC-ICP-MS分析方法是太子参水提液中金属元素形态分析的良好方法。ObjectiveTo develop a modern analysis method for speciation of metal elements(Cu,Mn,Zn) in Taizishen.MethodsThe contents of three elements in Taizishen and the extract of Taizishen were determined by ICP-MS,and the coupling of high performance liquid chromatography(HPLC) with inductively coupled plasma mass spectrometry(ICP-MS) was applied for elemental speciation.ResultsIn the extract of Taizishen,the contents of Cu,Mn,Zn were high,and the organic speciation of Cu,Mn,Zn were the main speciation.ConclusionThe HPLC-ICP-MS method is efficient to separate inorganic speciation and organic speciation of the metal elements in Taizishen.国家自然科学基金资助项目(No.20235020

西洋参多糖及总皂苷中无机元素的ICP/MS法测定

采用电感耦合等离子发射光谱—质谱联用法(ICP/MS),对西洋参样品、西洋参多糖、西洋参总皂苷中无机元素Mg、Al、P、Ca、V、Cr、Mn、Fe、Co、Ni、Cu、Zn、As、Se、Sr、Mo、Cd、Pb的含量进行测定分析。结果表明,西洋参多糖中各种元素的含量均明显高于西洋参样品,而不同方法提取的多糖中元素含量有一定差异;西洋参总皂苷中Cr、Ni、Cu、Zn、Pb元素的含量明显高于西洋参样品,而其它元素含量较低。这就为西洋参多糖、西洋参皂苷的综合开发利用及其药效提供理论依据

Preparation and Characterization of TiO_2 Nanotubes Array by Anodic Oxidation in Anionic Modified Glycerol-Based Electrolyte

本文采用电化学阳极氧化法以含氟的甘油和水混合溶液为电解液在纯钛表面制备了一层排列规整的TIO2纳米管阵列,研究了电解液中额外添加3种2价阴离子、不同的电解时间及不同的添加物浓度等因素对所获得的TIO2纳米管阵列形貌的影响。结果表明,在改性电解液中制备的TIO2纳米管阵列的长度均超过了未改性的电解液中制备的,并随着氧化时间的增长,纳米管管口直径增大,管壁变薄;同时添加的(nH4)2TIf6浓度在0.025~0.1 MOl.l-1范围内均可获得管长更长且形貌较好的TIO2纳米管阵列。High-order TiO2 nanotube arrays on titanium foils were prepared in glycerol-based electrolyte containing fluorine and water by electrochemical anodic oxidation in this work.The influence of different dianion additives,different oxidation duration and concentration of electrolyte additives on the effect of the morphology of TiO2 nanotube arrays were investigated.Results showed that the length of TiO2 nanotube arrays in the modified electrolyte were longer than the samples in the unmodified electrolyte.And with the growth of oxidation duration,the diameter of the nanotubes increased,the wall were thinner;moreover better and longer TiO2 nanotube arrays can be prepared in the glycerol-based electrolyte with the range of the concentration of(NH4)2TiF6.国家自然科学基金(No.30970887;30600149); 卫生部科学研究基金(No.WKJ2008-02-037); 福建省杰青项目(No.2011J06019); 教育部重点项目(No.209061); 福建省纳米材料重点实验室科学基金(No.NM10-03)资助项

ERK干扰质粒的构建及对胃癌细胞株BGC823 DcR3表达的影响

目的探讨胃癌发展的分子机制,通过构建ERK1/2shRNA真核表达载体,体外研究其对人胃癌细胞株BGC823ERK1/2蛋白及DcR3表达水平的影响。方法应用PRNAT-U6.1/Neo载体构建ERK1/2基因shRNA重组质粒,经脂质体法导入BGC823细胞中,分别设置对照组、干扰组和U0126抑制剂组。Westernblot法检测转染后BGC823细胞及抑制剂使用后ERK1/2蛋白的表达变化,荧光显微镜检测质粒自带GFP基因的表达情况确认转染效率,ELISA法检测各组细胞上清中DcR3分泌蛋白的表达特点。结果成功构建ERK1/2基因shRNA重组质粒。证明了ERK1/2蛋白的表达与DcR3的分泌水平在BGC823细胞株中呈正相关。结论 ERK1/2干扰质粒明显降低BGC823细胞的ERK1/2蛋白表达水平,ERK信号通路对DcR3的分泌具有重要调控作用,为其下游调控机制的研究奠定了基础

Determination of Mercury in Hair by Atomic Absorption Spectrometry with Pyrolysis

建立了一种采用固体热解塞曼原子吸收光谱快速测定毛发中痕量汞的方法 ,样品不需要经过消解即可直接测定。在优化的实验条件下 ,方法的相对标准偏差为3.9 %(峰面积)和7.2%(峰高) ,检出限为26ng/g(3σ) ,回收率为94 %～103 %。使用标准参考物质进行对照分析和通过与ICP -MS分析结果进行比较 ,结果令人满意。应用本法通过对实验动物样本的检测表明 ,动物毛发中汞的含量与其体内受汞毒害程度存在一定的相关性A rapid and simple method for the determination of total mercury in hair by using atomic absorpˉtion spectrometry with pyrolysis is developed.No sample digestion was needed.Under optimum conditions,the reproducibilities of the method were3.9%for peak area and7.2%for peak height,respectively.The detection limit was26ng/g(3σ)and the recovery was within the range of94%～103%.Several Standard Reference Materials were analyzed and the results obtained agreed well with the certified values.The perforˉmances of the method were compared with those of the inductively coupled plasma-mass spectrometry(ICP-MS)and excellent agreements were observed.国家自然科学基金资助项目(20175022) ;; 福建省科技攻关计划重大招标资助项目(2003Y005