Development and Application of a Novel Neutralization Test for Echovirus 25

目的:建立一种新型的快速、高通量的埃可病毒25型(ECHO25)中和抗体检测方法,并初步评价其在ECHO25中和抗体筛选和血清流行病学调查中的应用价值。方法:应用免疫荧光方法筛选ECHO25高亲和性抗体并将其作为检测单抗,结合酶联免疫斑点检测技术(ELISPOT)建立ECHO25中和抗体检测方法;使用不同效价的血清评价该方法的准确性;采用所建立的中和方法对ECHO25单克隆抗体、临床血清样品进行检测。结果:建立了快速检测ECHO25中和抗体的Nt-ELISPOT方法,以ECHO25单克隆抗体5B9作为检测抗体;相比经典的中和实验方法 Nt-CPE,该方法可显著缩短检测时间(从5~7 d缩短至1 d以内),检测结果具有较好的一致性;采用所建立的Nt-ELISPOT方法首次筛选获得3株对ECHO25具有较好中和能力的单克隆抗体;临床血清样品检测结果显示厦门地区可能存在ECHO25的流行。结论:该方法可以应用于中和抗体筛选和血清学的临床辅助诊断,为ECHO25的防治研究提供支持。Objective: To establish a rapid and high-throughput neutralization test for echovirus 25(ECHO25),and evaluate its application in neutralizing antibody screening and seroepidemiological surveys. Methods: Immuno-fluorescence assay was applied to screen a high affinity antibody, which was used as the detection antibody forECHO25, and a rapid neutralization test was established based on enzyme- linked immunospot assay(Nt-ELISPOT). The accuracy of this method was evaluated by detecting serum samples with different titer. Monoclonalantibodies against ECHO25 and clinical serum samples were detected via the established neutralization test. Results: A rapid method to detect neutralizing antibody against ECHO25 was established and an anti-ECHO25 anti-body, 5B9, was used as the detection antibody. The detection period could be shortened significantly comparedwith the classical neutralization test(Nt- CPE)(from five to seven days to less than one day), and the Nt-ELISPOT had good consistency with the Nt- CPE. Meanwhile, three neutralizing antibodies for ECHO25 werescreened firstly by this method. The detection results of clinical serum samples showed that infection of ECHO25 might be popular in Xiamen. Conclusion: This method can be used in neutralizing antibody screening and seroepi-demiological surveys, and it may provide support for the control of ECHO25.国家自然科学基金(81371817,81401669

肾移植术后淋巴漏及淋巴囊肿的诊治

[目的] 探讨肾移植术后淋巴漏或淋巴囊肿的诊断及治疗方法。[方法]回顾性分析21例肾移植术后淋巴漏（或囊肿）患者1，对淋巴漏或囊肿的可能原因进行探讨并通过引流液生化检查、乳靡试验和B超检查，分为非手术治疗和手术治疗。对诊断和治疗方法进行总结。[结果]21例中，17例为淋巴漏，4例为淋巴囊肿。6例单纯引流后治愈；15例经引流管注入无水酒精；其中9例治愈．6例治疗无效，最后经开放手术治愈。本组病例中，3例淋巴漏伴有感染，1例淋巴漏伴有移植肾急性排斥．经抗排斥治疗好转，淋巴漏治愈。[结论]肾移植术后淋巴漏或淋巴囊肿多与手术操作欠仔细或术中漏扎小淋巴管有关；多数病人可经非手术治疗痊愈，少数病人尤其淋巴囊肿形成者需开放手术治疗

中国陆地边境地区入境旅游市场的时空特征研究

本文通过对全国三大陆地边境旅游片区(东北、西北和西南)的入境旅游市场时空动态特征的分析,为我国陆地边境旅游发展战略、产品战略与营销战略的制定提供决策支撑。依据中国边境三大片区(东北、西北、西南)2003-2012年间客源市场的有关数据,尝试采用旅游地理、旅游市场分析原理及数理统计与实证分析等研究方法,从时间和空间两个层面对三大片区入境客源市场的年际变化、空间结构进行了深入系统的分析。研究结果显示:①三大片区的入境旅游在2003-2012年总体上呈现平稳上升的态势,入境客源量均呈现出一种拉伸型的"N"形曲线,且入境游客普遍停留时间较短;②入境客源市场空间分布极不均衡,且呈现地域近邻性特征:东北片区入境客源市场主要集中在以俄罗斯为代表的东北亚国家;西北片区主要集中在以哈萨克斯坦为代表的中亚国家;西南片区则主要集中在以越南为代表的东盟国家。3个片区的国际旅游市场均有待开拓

Preparation and Properties of δ-MnO_2

用过二硫酸铵((NH4)2S2O8)氧化Mn(Ⅱ)盐制备-δMnO2。比较了反应过程中Mn2+与OH-的摩尔比、反应温度、(NH4)2S2O8用量等因素对MnO2晶型的影响。用X射线衍射(XRD)、红外光谱(IR)分析了制得的-δMnO2的结构。测试了样品的热性能和循环伏安、恒电流充放电性能。结果表明所制备的-δMnO2具有良好的层状结构,热稳定性能较好,放电容量较高(约160mA.h/g,2.0~4.5V)。δ-Manganese dioxide(δ-MnO_2) was prepared from a Mn(Ⅱ) salt oxidized by ammonium persulfate ((NH_4)_(2)S_2O_8). The crystalline form of the δ-MnO_2 was affected by the molar ratio of Mn~(2+) to OH~(-), (reaction) (temperature), and the amount of (NH_4)_2S_2O_8. The structure of the δ-MnO_2 was characterized by means of X-ray (diffraction)(XRD) and Infrared spectroscopy(IR). Its electrochemical performance was also (studied) via cyclic voltammetry and constant current charge/discharge experiments. The results indicate that (as-prepared) δ-MnO_2 has a good layer-structure and a high discharge capacity(~160 mA·h/g, 2.0~(4.5 V)).福建省教育厅资助项目(JB02193

一例白鲸应激反应综合症的病例报道

本文就一例幼年雌性白鲸在运输后发生的应激反应综合症进行了诊断、治疗。临床表现为精神不振、拒食、体重下降、体色暗淡、巩膜及结膜充血、皮肤瘙痒;血液学检查表明轻度贫血和脱水、肌肉炎症及胃炎。后进行综合措施调整后,4d后恢复采食,2周后康复。同类鲸的疾病研究国内文献未见报道