Research on Buddhism Repertoire of Yuan Drama

元杂剧作为元代社会文化语境产物，存在一些宣扬佛教思想的杂剧。对这些杂剧中所表现的佛教思想进行探讨，可以使我们从中领略到其社会教化意义。本文试图从佛教理论这一视角对元代戏曲，尤其是元杂剧进行分析。 全文共分为前言、正文、结语三大部分。 第一部分是前言，简要回顾了前人相关的研究成果，并论证了用佛教理论研究元杂剧的可行性。 第二部分为正文，分为三章。第一章主要探讨佛教思想与元杂剧之因果报应。我们认为因果剧是作家用来表现佛教因果报应的观点，并融合儒家思想构思故事情节，表现现实人生的一种手段。第二章主要是元曲佛教思想之“度脱剧”举要及解析。在给出了度脱剧的基本概念和代表作品之外，我们认为度脱剧中体...Yuan Drama as a socio-cultural context of the Yuan dynasty, convey the drama of Buddhism exist, to explore the Buddhist thought of drama, from which allows us to enjoy the significant impact on the social education. This article attempts to Buddhist theory perspective on the drama in the Yuan dynasty, in particular analysis of the Yuan dynasty. This article is divided into four parts. The first pa...学位：文学硕士院系专业：人文学院_戏剧戏曲学学号：1022008115375

Construction and identification of interference plasmid targeting on TNFAIP8

目的:构建并筛选出干扰效率最佳的TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒。方法:通过生物软件选择3个TnfAIP8基因干扰位点,构建干扰质粒并测序验证,将干扰质粒及对照质粒分别转染至A549细胞,通过rT-PCr、WESTErn blOT检测干扰效率。结果:经rT-PCr和WESTErn-blOT证实TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒能有效干扰并抑制细胞内TnfAIP8基因的表达,通过流式检测发现降低TnVAIP8表达可以提高细胞对A dr5SC fV诱导凋亡的敏感性。结论:成功构建和设计了对TnfAIP8基因具有显著干扰效率的干扰质粒,为进一步研究TnfAIP8基因的功能奠定了基础。Objective: To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-p SIRENRetro Q.Methods: Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-p SIREN-Retro Q interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results: We successfully design and built three TNFAIP8-shRNA-p SIREN-Retro Q interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.国家自然科学基金项目(81272720); 福建省卫计委医学创新课题(2014-CXB-43); 厦门市科技计划项目(3502Z2083008)资

Progress in Researches on Diphenylarsinic Acid Pollution of Soil-water Environment and Its Remediation

Chemical warfare agents containing organoarsenic compounds such as Clark I (diphenylcyanoarsine) and Clark II (diphenylchloroarsine) were widely produced and used during World Wars I and II. After the wars, remains of these agents were simply dumped into the sea or buried underground, thus inevitably polluting the soil-water environments of the sits where they were disposed with the arsenic contained in the chemical weapons. In the environment, these abandoned chemical agents are easily hydrolyzed and oxidized into diphenylarsinic acid (DPAA), rather stable in structure, and other organoarsenic compounds. So far, DPAA has been detected in quite a number of the areas where these chemical weapons were dumped. The detection has aroused extensive concerns because the presence of DPAA may bring about environmental and health risks. Scholars both at home and abroad have already begun doing some researches, trying to find ways to analyze DPAA in the soil and water environments, determine their status and behaviors and remedy the polluted environments. However, few have done any to summarize systematically progresses in the research. In this paper, a review is presented to introduce some high-effect inorganic and organic extractants and GC as well as LC analytical methods for DPAA in the soil, and sources and status of the pollutant in the soil-water environments. Generally speaking, the DPAA contaminated areas are located mainly in Northeast China, and South and Southeast Japan. Especially in the chemical weapons dumping sites, the concentration of total arsenic is far beyond the criteria for safety. At the same time, the paper also discusses how DPAA is adsorbed/desorbed, translocated and transformed in the soil-water environment, what are the factors affecting the processes and what are the mechanisms. Studies in the past reported that the adsorption/desorption of DPAA in soil was controlled by a variety of factors, including pH, inorganic ions, Fe/Al oxides, organic matter, redox potential (Eh), etc. and adsorption of the substance was completed via ligand exchange reactions between hydroxyl groups of Fe/Al oxides and arsenate of DPAA, rather than the hydrophilic effect of organic matter; the effective transformation of DPAA in the soil occurred under flooded anaerobic conditions, and under sulfate-reducing conditions, in particular; and iron reduction and sulfate reduction were the two key factors controlling desorption and transformation of DPAA. In the end, the paper elaborates the physical, chemical and biological technologies available for remediation of DPAA contaminated soil-water environments, and their remediation efficiency, controlling factors and mechanisms as well. In terms of physic-chemical remediation, application of activated carbon, Fenton and Fenton-like oxidation and photochemical degradation has been demonstrated to be able to effectively remove DPAA in soil-water environments. In terms of bioremediation, certain progresses have been made, like screening of highly efficient DPAA degrading bacteria, unfolding microbial remediation and combined microbial-phytoremediation and previewing directions of the future researches. The paper holds that all the relevant research findings will serve as theoretical reference for future in-depth studies on DPAA pollution of soil-water environments, remediation of DPAA polluted environments, and protection of environmental quality and human health from DPAA pollution. For further researches, emphases should be laid on the following aspects: (1) To perfect quality assurance and quality control system for DPAA analytical methods, with focus on development of standard alternatives, purgation of internal standards and markers; (2) To launch investigations on scope and extent of DPAA contamination, while taking into the consideration of geographical locations, soil types and land-use patterns of the chemical weapon burial sites; (3) To explore forms of DPAA bonding with soil colloids, clay minerals and oxides in the soil and molecular binding mechanisms, and elucidate the mechanisms responsible for adsorption/desorption, translocation and transformation of DPAA in multi-media environment and at microscopic interfaces; (4) To explore for develop new remediation materials, intensify researches on physic-chemical-phyto combined remediation and continue to screen out highly efficient DPAA degrading bacteria and probe mechanisms of their effectiveness at molecular as well as genetic levels, while integrating genetic engineering, molecular biology with phytoremediation technologies, so as to eventually establish a bioremediation technical system applicable to DPAA contaminated media different in type and condition

多变量抗堵塞滴头

本发明涉及一种多变量抗堵塞滴头，其出水流量可调节、抗堵塞，即装即卸易维修，表层输水，深层灌溉，高效节水灌溉，进一步提高了干旱地区灌溉水的利用率，缓解灌溉水资源不足的矛盾。本发明包括与输水管连接的滴头，滴头包括滴头套，滴头套由可拆卸连接的滴头上套和滴头下套构成，滴头套的腔内插有变量过滤管，变量过滤管两端分别卡入滴头腔上下缩口内，滴头套并与地下引水管连接，滴头套下端设置有连接咀，滴头套与地下引水管通过连接咀连接，连接咀出水孔壁内设置有变量阀，地下引水管管壁内设置有变量滴头，变量过滤管和变量滴头的管孔内镶有海绵状材料

用于山地的移动式节水灌溉装置

本实用新型涉及一种节水灌溉装置，尤其是涉及一种用于山地的移动式节水灌溉装置。目前，由于缺乏与山地相配套的高效节水灌溉设备，只能是被动地采取传统的漫灌浇地方式灌溉，由于地表蒸发量大，传统漫灌方式灌溉水的利用率低，致使至今山地农业生产仍未摆脱靠天吃饭的局面。本实用新型提供一种用于山地的移动式节水灌溉装置，其特征在于：包括带有注水口的储水器，储水器底部连接有导流水管嘴，导流水管嘴上接有滴灌软管，滴灌软管上设置有水流控制器。与现有技术相比，具有的优点为：结构简单，易制造，成本低廉；实施不受地形条件的影响，水源多少的限制，易于推广使用；滴灌水既无地表蒸发，又不破坏土壤表层疏松结构，水利用率极高

一种控水渗灌袋

本发明涉及一种控水渗灌袋，其实现了将有限的灌溉水能持续不段地供给根际局部土壤，使造林穴内土壤长时间保持较好的湿度，确保干旱地区造林成活，结构简单，节水效果好。本发明包括控水渗灌袋本体，渗灌袋本体下端设置有自锁扣，自锁扣下部连接咀上插接有地下引水管，在地下引水管管壁内插接渗灌头，控水渗灌袋本体上部设置有带自封条的注水口，在自锁扣腔壁内插有过滤管，过滤管内镶过滤材料，控水渗灌袋本体采用聚乙烯复合膜热压封口制成

泉州地区肝炎患者TTV感染状况调查

近年来,人们注意到除HAV、HBV、HCV、HDV、HEV和HGV等肝炎病毒外,仍有5％～10％的肝炎患者不能确定原因。1997年底Nishizawa等使用代表性差异分析法(RDA)首次从1例非A～G型的输血后肝炎患者血清中分离到一种新的单链DNA的病毒基因,暂命名为输血传播病毒(transfusiontransmitted virus,TTV)。我们根据公布的TTV序列,设计了两对特异性引物,建立了检测血清TTV DNA的巢式PCR方法,对泉州地区各类型肝炎患者TTV感染状况进行调查,报