Methodologic Study of HPCE Fingerprints of Radix paeoniae Alba and Radix paeoniae Rubra

目的确定赤芍和白芍的高效毛细管电泳分析方法,建立赤芍和白芍的高效毛细管电泳法(HPCE)指纹图谱。方法HPCE工作条件:采用未涂层熔融石英毛细管(内径75μm,有效长度50 cm),分离电压为25 kV,柱温25℃,二极管阵列检测器(DAD)检测波长为220 nm,缓冲液为30 mmol/L硼砂(pH=9.0)溶液。按此条件对来自不同产地的7种赤芍样品和8种白芍样品进行了分析。结果建立了赤芍和白芍HPCE指纹图谱,采用中药指纹图谱相似度计算软件,以系统生成的对照指纹图谱为对照模板对不同样品的图谱进行相似度计算。结论该方法简捷、有效,可以用于赤芍和白芍药材的质量控制。Objective To set up a modern High Performance Capillary Electrophoresis analysis method for separating and detecting Radix paeoniae Alba and Radix paeoniae Rubra and establish their fingerprints.Methods Seven samples of Radix paeoniae Alba and eight samples of Radix paeoniae Rubra were carried out by HPCE under the following conditions: bared fused silica capillary(50 cm ×75 μm i.d.),30 mmol/L borate(pH = 9.0) as buffer,the run voltage is +25 kV,detection wavelength of UV at 220 nm,and column temperature of 25℃.Results The fingerprints were confirmed and compared by the software of the similarity evaluation system for chromatographic fingerprint.Conclusion The results showed that the method of HPCE fingerprint is reliable and accurate to control the quality of two Chinese traditional medicines.国家自然科学基金重点项目(No.20235020);; 青岛“2004将才计划”(No.04-3-JJ-11);; 青岛市崂山区科技计划项目(No.LS-05-KJZX-76

A New Mathematics Model of the Temperature Programmed Analysis Technology

以表面作用包括表面吸/脱附以及表面反应本征速率方程为基础,提出新的程序升温分析技术(TPAT)的数学模型。与经典的理论模型相比,这种新的TPAT理论模型更接近实际的程序升温分析实验过程。设计和进行特定催化剂的TPd、TPr及TPO实验,得到相应的程序升温谱图,采用新的TPAT理论模型模拟上述谱图,计算出相应的表面作用活化能等重要热力学参数。结果表明,新的理论模型具有良好的模拟性能,平均相对误差(Ard)小于1%。Based on the surface effect including surface adsorption/desorption and intrinsic kinetics rate equation,a new mathematical model of the temperature programmed analysis technology was proposed.This model is different from the classical TPAT theory models,which indicates much more coincidence with the actual reactions than other models.TPAT(TPD,TPR and TPO) experiments were designed and carried out to receive their profiles.Based on these profiles of TPAT and the simulated experiments,the novel theory model was designed and the thermodynamics parameters(such as activation energy,etc.) were deduced and calculated by this model.The results show that this model has excellent simulation with the actual experiments,and the average relative errors are easily controlled less than 1%

Evaluation of DPPH Free Radical Scavenging Capability of Hippocampus japonicus Kaup. and Their HPLC Fingerprint

目的采用体外1,1-二苯基苦基苯肼(dPPH)抗氧化模型对海马提取物的抗氧化性质进行评价,并建立小海马HPlC特征指纹图谱,用于小海马药材的鉴别及质量评价。方法利用离线dPPH抗氧化评价体系对海马不同提取物进行评价,结果表明,海马水提物抗氧化能力最强,在此基础上又探明了海马水提物抗氧化能力随时间和浓度的变化规律,为海马抗氧化活性提供了科学依据。依据抗氧化活性实验结果,建立了海马水提物HPlC特征指纹图谱分析方法。结果海马水提物大部分化合物达到基线分离,方法的精密度、重现性、稳定性良好;建立小海马药材HPlC指纹图谱,采用中药指纹图谱相似度计算软件,对小海马进行真伪辨别和质量评价。结论表明该方法简捷、有效,是小海马药材鉴别及质量控制的有效方法。OBJECTIVE To evaluate the antioxidant activity of the extracts of Haima,and to establish the HPLC fingerprint of Haima for the discrimination and the quality evaluation of Haima.METHODS Antioxidant properties of Haima extracts with different solvent were assayed in terms of antioxidant activity by scavenging activities on 1,1-diphenyl-2-picrylhdrazyl(DPPH).The effect of time and concentration of Haima extract on the antioxidant activity was also studied.Secondly,a chromatographic fingerprint method was developed.RESULTS The antioxidant activity of water extract of Haima was higher than all the other extracts of Haima.The developed HPLC method was simple,accurate and reliable for the development of Haima fingerprint.Ten Haima samples collected from different medicine store were analyzed and the Haima HPLC fingerprint was established.The similarity of the HPLC chromatogram was performed for authentication and quality control of Haima.CONCLUSION The HPLC fingerprinting techniques have high potential in authentication or source-tracing types of applications.国家自然科学基金重点项目(20235020);青岛市共建生物医药研发测试中心资助项目(LS-05-KJZX-76

Substituent effect on the assembly of coordination polymers containing isophthalic acid and its derivatives

通讯作者地址: Yang, SY (通讯作者), Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surface, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surface, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. W Anhui Univ, Dept Chem, Luan 237012, Peoples R China 电子邮件地址: [email protected] coordination polymers [Zn4(H2O)(ip)4(py)6]n1, {[Zn2(hip)2(py)4]2·(py)}n2, [Zn(tbip)(py)2]n3 and [Mn(tbip)(py)2]n4 (H2ip = isophthalic acid, H2hip = 5-hydroxyisophthalic acid, H2tbip = 5-tert-butylisophthalic acid, py = pyridine), have been hydrothermally synthesized and characterized. The photoluminescent properties of 1–3 have also been investigated. For 1–3, as substituents change from H, OH to tert-butyl, the coordination numbers of Zn2+ ions decrease, and the dimensionalities of the structures of 1–3 decrease from 2D (4,4) net, 1D double stranded chain to 1D chain. For 3 and 4, as Mn2+ tends to have a higher coordination number as compared to Zn2+, the structure of 4 is a 2D (4,4) net while the structure of 3 is a 1D chain. The substituents on isophthalic acid influence the coordination environments of metal ions and the coordination modes of the carboxyls, and thus determine the structures of the coordination polymers. The coordination behavior of metal ions also affects the formation of the structures.National Natural Science Foundation of China 20471049 20721001 20725310 20673085 National Basic Research Program of China (973 Program) 2007CB815301 2007CB81530

福建外来船舶压舱水中浮游植物种类组成与丰度及其影响因素的初步研究

2006~2008年间对进入福建沿海4个主要港口的12艘国际航船所携带的压舱水进行浮游植物种类组成和丰度分布的调查.此调查共检测出浮游植物7个门86属239种(含变种和变型),77μm孔径网滤样和20μm孔径网滤样的平均藻类丰度分别为1.2×102cells/dm3(变动在0~9.1×102cells/dm3间)和3.4×103cells/dm3(变动在0~3.0×104cells/dm3间).同时对其中6艘船舶的压舱水样品用f/2培养基培养,共有13种硅藻和1种甲藻被成功培养.文中还分析了压舱水中浮游植物丰度与盐度、水龄的关系,并结合历史资料讨论了压舱水里各类浮游植物的分布

中国东南沿海港口外轮压舱水生物的调查

随机选取东南沿海港口的17艘外来船舶(含8条集装箱船和9条散货船)作为监测对象,进行压舱水浮游植物及动物的物种鉴定和丰度测定,并对监测数据进行统计学分析。检出分属于7个浮游植物门类和5个动物门类的309种外来压舱水生物(包括60种赤潮生物)。外轮压舱水生物的分布及生存状态与水样的水龄和盐度相关。船舶压舱水排放是大家熟知的外来水生生物入侵的主要媒介。本调查结果表明,中国东南沿海外来散货船的压舱水排放所具的潜在生物入侵风险比集装箱船更应受关注

Damage to mangroves from extreme cold in early 2008 in southern China

通讯作者Author for correspondence (E-mail: [email protected])2008年初,我国南方19个省经历了50年一遇的持续低温雨雪冰冻天气。极端气候对华南沿海各省的红树林区造成不同程度的危害。2008年3月,在我国南方各省红树林区的10个代表性地点,对这次寒害造成的红树植物伤害程度进行了系统的调查。结果表明:冬季低温对红树林的影响极为显著,特别是在低纬度的海南、广西和广东湛江,由于极端低温正值夜间退潮,对红树林的影响更为显著;在纬度较高的福建,本地红树种类秋茄(Kandelia obovata)、桐花树(Aegiceras corniculatum)和白骨壤(Avicennia marina)及引种的木榄(Bruguiera gymnorrhiza)等,由于长期适应于冬季较低的气温或在种植前经过抗寒锻炼,具有较强的抗寒能力。各地主要红树植物中,广布种秋茄、桐花树和白骨壤最为耐寒,其耐寒性均大于红树科的木榄、海莲(Bruguiera sexangula)和红海榄(Rhizophora stylosa)。海桑(Sonneratia caseolaris)对温度的敏感性最强,抗寒能力最低,因此,即使在其原产地海南也受到较为严重的寒害,在纬度更高的引种地出现大面积受害甚至全部死亡,而从孟加拉国引种的无瓣海桑(Sonneratia apetala)却显示出一定的抗寒能力。同一地点的红树植物幼苗的抗寒能力低于大树。此次寒害也造成了苗圃场的种苗大量死亡,成熟的植株提前落花落果,这势必会影响后继一两年内红树林的自然更新和人工造林。因此,在未来红树林造林或人工引种中,一定要考虑到红树植物的抗寒能力。国家自然科学基金(30671646和30700092);厦门大学“闽江学者”启动基

C3醇电氧化过程中铂电极的表面结构效应和反应物的分子结构效应：循环伏安法和原位FTIR反射光谱联用的研究

学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：MJ10013

基于支持向量机的干散货航运市场运价预警

为分析预测干散货航运市场运价波动的警情,建立基于支持向量机的运价预警模型,并构造相应的算法。选择波罗的海海岬型指数(Baltic Capesige Index BCI)、波罗的海巴拿马指数(Baltic Panamax Index BPI)、波罗的海灵便型指数(Baltic Supramax Index BSI)、波罗的海小灵便型指数(Baltic Handsige Index BHSI)等四个干散货运价指数作为警兆指标,结合航运专家知识经验,确定干散货航运市场运价的实际警度。依据训练样本数据,利用支持向量机的学习功能,通过编制MATLAB软件程序,获得市场运价警度的分类超平面及预测警度区间,并进行内插和外推检验。检验结果表明此方法对于干散货航运市场运价预警有很好的适用性

基于衔接组合的集装箱多式联运服务分段采购优化

为解决集装箱多式联运服务分段采购中的运输费用和运输时间优化难题,从多式联运经营人的视角出发,提出了集装箱多式联运总费用最小化和总运输时间最小化的双层优化目标.基于运输路径选择和时、空、量的衔接组合,结合托运人运单、集装箱多式联运组织形式、集装箱交接地点、运输路段及方式、运输时间、运费率等要素约束,建立了集装箱多式联运服务分段采购优化模型.运用自然约束语言设计搜索算法程序并求解.数值实验分析结果表明,该模型及搜索算法不仅易于实施求解,而且应用效果良好,达到了集装箱多式联运一体化无缝运输的目的