2,647 research outputs found
hnRNPA2/B1 regulates apoptosis-related gene PLK3 expression and BIM alternative splicing in breast cancer cells
目的:核糖核不均一蛋白(hnRNPA2/B1)作为一个可变剪切子,在乳腺癌组织中过度表达,参与细胞多种生理过程。本文研究乳腺癌细胞中hnRNPA2/B1调控凋亡相关基因PLK3表达和BIM的可变剪切。 方法:通过CRISPSR-Cas9技术敲除乳腺癌细胞MCF-7和MDA-MB-231中hnRNPA2/B1基因后,验证凋亡相关基因的表达;结合转录组测序分析以及RTPCR验证凋亡相关基因是否发生可变剪切,并通过RNA免疫沉淀技术检测hnRNPA2/B1蛋白是否与凋亡相关基因的pre-mRNA结合。体内实验中用Tunel法检测MCF-7细胞hnRNPA2/B1敲除前后小鼠移植瘤凋亡变化。通过CC...Objectives: As a splicing regulator, Previous studies found hnRNP A2/B1 overexpression in breast cancers associated with various physiological processes of cells. Our studies are to illustrate hnRNPA2/B1 regulates apoptosis-related gene PLK3 expression and BIM alternative splicing in breast cancer cells. Methods: We knocked out hnRNP A2/B1 by CRISPSR-Cas9 technique in MCF-7 and MDA-MB-231 cell, c...学位:理学硕士院系专业:医学院_微生物学学号:2452014115346
Research on Urban-Rural Verkehr, Human Development and Chinese Urban-Rural Income Gap
刚过去的“十二五”时期中国经济总量稳居世界第二,人均国内生产总值增至7654美元。改革开放以来,我国经济发展成果世界瞩目,居民生活水平和质量得到显著提高。然而在经济高速发展的同时,我国城镇和农村二元经济结构愈发明显,特别是收入差距逐渐扩大,虽然近年来略有下降,但仍处于有风险的差距水平。城镇和农村收入差距导致了一系列的社会问题,农村消费不足、城乡心理失衡、二元特征更加明显等等。党和政府高度重视城镇和农村差距问题,在“十三五”规划中不仅提出了国内生产总值和城乡居民人均收入比2010年翻一番的总体目标,同时强调要推动城镇和农村协调发展,缩小城镇和农村的收入差距。要实现城乡协调发展,就得找到症结所在,...China has been remaining the largest economy stably over the Twelfth Five-Year Plan period. Though China has the largest population, Chinese per capita income has increased to about 7800 dollars. Since Chinese Reform, Chinese economic development has been drawing worldwide attention for people’s living standard and quality are improved notably. Meanwhile, the characteristic of dual economic struct...学位:经济学博士院系专业:经济学院_政治经济学学号:1532013015399
The Basic Study on Rare Earth Nd3+ Ion Doped Polymer Optical Waveguide Amplifier
近年来,有机聚合物材料以其独特的性能、低廉的成本受到越来越多的重视。聚合物光电子器件如阵列波导光栅、电光调制器、光开关和光波导放大器等几乎已经涉及了集成光学的各个领域。光波导放大器的原理是利用掺入光波导中的稀土离子在泵浦光作用下产生受激辐射来实现信号光的放大。本论文以三元钕配合物Nd(TTA)3(TPPO)2作为有源材料,制备了硅基聚合物光波导放大器,研究了器件在1060nm波长处的增益特性。所做的具体工作如下: 1.合成了三元钕配合物Nd(TTA)3(TPPO)2(HTTA:2-thenoyltrifluoroacetone,TPPO:triphenylphosphineoxide),将其...In recent years, organic polymer material has been attracting more and more attention because of its unique performance and its low cost. Polymer optoelectronic devices such as arrayed waveguide gratings, electro-opticmodulators, optical switches and waveguide amplifiers has been involved in almost every field of integrated optics. The waveguide amplifer make use of the rare earth ions incorporat...学位:工学硕士院系专业:信息科学与技术学院_工程硕士(电子与通信工程)学号:2312013115312
Design and Implementation of Passive Optical Network Management System for a Community Ethernet
随着通信技术的迅速发展,用户对带宽的需求越来越高。以成熟的以太网技术为基础的以太无源光网络(EPON)采用点到多点结构、无源光纤传输,能够在以太网之上提供多种业务。相对于传统的穿射设备以及技术而言,EPON其更加便于维护,承载业务更为灵活,因此是未来现代宽带入网的最佳选择之一。 虽然EPON设备都自带有网管系统,但是也存在着多种问题:首先,EPON网元管理系统只能分散管理自己的设备,不同厂商其设备存在较大差异性,从而造成了设备之间缺乏兼容性,没有办法实现各个设备之间的有效联结。其次,不同时期采用的网络体系结构、技术及通信监控网络差异很大,这就造成了对于设备的维护以及监督效能的降低,提升了设备...With the rapid development of communication technology, the user demand for bandwidth is more and more high. Ethernet passive optical network with Ethernet technology based (Ethernet Passive Optical Network, EPON) with point to multipoint architecture, passive optical transmission, can provide multiple services over Ethernet. It combines the advantages of passive optical network technology and Eth...学位:工程硕士院系专业:软件学院_工程硕士(软件工程)学号:X201323224
The Components and Promoting Mechanism of the Public Service Dynamic Adaptability in the Customs
伴随国家间竞争的加剧以及公民权利意识的觉醒,公共服务面临的环境挑战越来越复杂多变,在此背景下,如何更好地适应环境、解决公共服务能力建设中存在的问题成为政府部门亟需解决的重大问题,这也成为了海关谋求提升自身服务能力的内在动力。 近年来,海关以“服务型”海关为目标,强调“把好国门”、“做好服务”,大力推进公共服务能力建设,不断适应管理相对人的服务需求和其他环境因素的变动,积极提高公共服务水平,但由于科层制固有的缺陷以及部门本位思想的残存,海关公共服务能力建设中仍然存在有对环境敏感度不高、对管理相对人需求回应迟缓等问题。对此,笔者认为,海关在公共服务能力建设当中可以借鉴动态能力理论,从动态能力的研...Due to the aggravation of countries’ competition and the awakening of citizens’ right consciousness, Customs public service is facing plenty of challenges which come from complicated environment situations. In this background, “How to better adapt to the environment” and addressing the existing issues of the public service ability became government’s priority problems which urge Customs to seek fo...学位:公共管理硕士院系专业:公共事务学院_公共管理硕士学号:1512014115034
Financial Analysis And The Value Of Comparative Study About TianQi Co.,Ltd And GanFeng Co.,Ltd
近年来,受到国家政策支持和下游新能源市场需求的双重带动,锂电企业保持着快速发展和优秀的创收能力,尤其是2016年,以天齐锂业和赣锋锂业为代表的锂电企业不仅在经营上取得了优异的成绩,在资本市场上也表现耀眼。短期内,受市场需求旺盛的影响,锂电企业仍将处于高景气度,但是锂电企业股价在资本市场已有巨大涨幅,对于投资者来说呢,它们是否有继续投资的价值,值得分析和关注。 本文着重从财务角度出发,通过战略、财务和估值分析,多角度了解天齐锂业和赣锋锂业公司的经营状况、发展潜力和投资价值。通过本文分析,可以发现两家企业在发展过程中采用着不同的经营战略,天齐锂业公司采用差异化战略、而赣锋锂业公司则通过纵向一体化...In recent years, Driven by policy and demand, lithium enterprises have maintained rapid development and excellent income. Especially in 2016, such as TianQi Co.,Ltd and GanFeng Co.,Ltd, they have acheived excellent performance not only in the business but also in the capital market. In the short term, by the impact of macro demand, lithium companies will remain in a high degree of prosperity. But ...学位:工商管理硕士院系专业:管理学院_工商管理硕士(工商管理硕士)学号:1792015115156
China's Green Economic Growth and the Corresponding Energy Transformation
资源耗竭和环境污染已经成为中国可持续发展的严重制约,特别是雾霾等环境问题的大面积集中爆发,更是将这一问题摆在了公众和政府的面前。但是,对于中国这样的发展中大国,“发展是第一要务”,因为解决十三亿人口的吃饭和就业问题都离不开经济较快增长。如何在稳增长、资源节约、环境友好这三者之间的权衡取舍是政府面临的难题。因此,对于中国社会经济的可持续发展,关键是在兼顾经济增长的同时尽可能实现资源节约和环境改善。这就需要实现绿色经济增长的转型。 另一方面,气候目标成为中国经济发展的约束条件。在2014年11月的中美联合声明中,中国首次提出“计划2030年左右二氧化碳排放达到峰值且将努力早日达峰”,这是中国政府...Resource depletion and environmental degradation have become big challenges for China's sustainable development. In particular, as the terrible haze appeared in large areas of China, resources and environmental constraints become an unavoidable problem. However, as the largest developing county with over 1.3 billion people, economic development is the first priority, which is the requirement of em...学位:经济学博士院系专业:经济学院_能源经济学学号:3132012015366
In situ investigate typical polycyclic aromatic hydrocarbons (PAHs) distributed on the root epidermis and its processes transported into tissues
红树植物根去除外界环境(气相、沉积物相和水相)中多环芳烃(PAHs)是环境中PAHs的重要去除途径。因此,大量研究围绕解决环境中(大气、水和沉积物)PAHs进入植物体的途径、在植物体内的迁移路径、赋存情况等一系列相关问题展开。然而,因缺乏合适的红树植物根表皮PAHs的原位分析方法,对于PAHs在红树植物根表皮赋存情况及向组织内部迁移这一红树植物根去除PAHs步骤尚不清楚。鉴于此,本文开展了相关研究,分别构建了激光诱导纳秒时间分辨荧光光谱法(LITRF)、导数激光诱导纳秒时间分辨荧光光谱法(D-LITRF)以及显微荧光光谱法(MFSA)法实现了红树植物根表单/双组分PAHs的原位定量测定,并初步...The depuration of PAHs by mangrove plants in the external environment (gas phase, sedimentary phase and water phase) was one of the important ways to remove PAHs. Therefore, there existed many studies focused on investigate a series of related processes such as the transport pathways and distribution of PAHs in plant. Among them, the process and mechanism of PAHs located on the epidermis and trans...学位:理学博士院系专业:环境与生态学院_环境科学学号:3312014015398
A Report on the Translation of Introduction and Chapter One of Graphene: Fundamentals, Devices and Applications
当代语言学重视对语言系统的机制以及实际使用的研究,其研究成果推动了翻译研究向深层次的语言研究方向发展,并且促进了比较科学合理的翻译理论体系的建立。元话语理论自1959被提出以来,多位学者对其进行了不同视角的论述。经过50多年的发展,元话语理论的内涵逐渐丰富与完善,尤其是Hyland(2005)基于以往学者的观点再次定义了元话语概念,提出了元话语人际分类模式,并且探讨了许多与元话语相关的问题。他的分类模式也逐渐被应用到不同领域。由于元话语理论本身讨论的是作者-读者关系,所以可以使用到翻译研究中,用来探究原文(作者创作)与译文(读者阅读)之间的关系,故此元话语理论能为翻译的研究与实践提供实际可操作...Modern linguistics puts great emphasis on the study of the mechanisms of language system and its actual use, thus promoting translation studies to shift to in-depth linguistic research and resulting in the establishment of a more scientific and reasonable theoretical system of translation. Ever since 1959 when metadiscourse was coined, many scholars have tried to interpret the concept from differe...学位:翻译硕士院系专业:外文学院_翻译硕士学号:1202014115271
The analysis of securities margin trading’s impact on china stock market volatility
2010年3月31日,融资融券交易的正式推出,结束了我国股票市场长期以来的“单边”格局。对于融资融券交易机制的引入能否提高我国股票市场的运行质量,学术界及业内人士的看法不一。鉴于此,本文通过选取上海股票市场的相关交易数据进行分析,试图探究推出融资融券交易对于国内股票市场的影响。 本论文首先整理并分析国内外融资证券交易的相关资料,确定研究思路和方向;其次,对融资融券含义、发展过程、交易方式、对市场的影响作用,股票市场波动性、流动性的影响因素及融资融券交易对于股票市场流动性和波动性影响进行具体解析,为实证研究奠定基础;然后,以上海股票市场作为研究对象,利用VAR模型、格兰杰(Granger)因果...On March 31,2010,the official launch of securities margin trading, ended the China Stock Market "long-term unilateral" pattern. Whether the introduction of securities margin trading can improve the operation of China Stock Market,academics and insiders have different opinions. In view of this, the paper attempts to explore the influence of securities margin trading by analyzing the deal date of Sh...学位:金融硕士院系专业:经济学院_金融硕士学号:1562013115213
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