目的：核糖核不均一蛋白（hnRNPA2/B1）作为一个可变剪切子，在乳腺癌组织中过度表达，参与细胞多种生理过程。本文研究乳腺癌细胞中hnRNPA2/B1调控凋亡相关基因PLK3表达和BIM的可变剪切。 方法：通过CRISPSR-Cas9技术敲除乳腺癌细胞MCF-7和MDA-MB-231中hnRNPA2/B1基因后，验证凋亡相关基因的表达；结合转录组测序分析以及RTPCR验证凋亡相关基因是否发生可变剪切，并通过RNA免疫沉淀技术检测hnRNPA2/B1蛋白是否与凋亡相关基因的pre-mRNA结合。体内实验中用Tunel法检测MCF-7细胞hnRNPA2/B1敲除前后小鼠移植瘤凋亡变化。通过CC...Objectives: As a splicing regulator, Previous studies found hnRNP A2/B1 overexpression in breast cancers associated with various physiological processes of cells. Our studies are to illustrate hnRNPA2/B1 regulates apoptosis-related gene PLK3 expression and BIM alternative splicing in breast cancer cells. Methods: We knocked out hnRNP A2/B1 by CRISPSR-Cas9 technique in MCF-7 and MDA-MB-231 cell, c...学位：理学硕士院系专业：医学院_微生物学学号：2452014115346
