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雄激素不敏感综合征（AIS）是临床上较为常见的男性假两性畸形，属于性发育障碍疾病（DSD）的一种。患者的染色体核型为46，XY，目前认为是一种伴X连锁隐性遗传性疾病，患者可表现为一系列雄激素抵抗性临床症状，从无生育能力但外貌正常的女性到完全正常表型有原发性不育或者无精少精症的男性不等，给患者的身心健康带来严重的影响。福建省自然科学基金杰青项目（2015D012

Analysis on gene detection results of 3715 cases with thalassemia in Xiamen

目的:调查厦门地区地中海贫血基因的携带率、基因突变类型及其频率分布。方法:对2013年1月~2014年8月在该院住院或门诊治疗的患者及健康体检人员共3 715例进行地中海贫血基因检测,对检测结果进行统计分析。结果:检测出静止型α-地中海贫血200例(5.4%),中间型α-地中海贫血44例(1.2%),标准型α-地中海贫血1 213例(32.6%),β-地中海贫血787例(21.2%),α-合并β-地中海贫血69例(1.8%)。结论:不同就诊对象地中海贫血检出率具有明显差异,血常规是筛查地中海贫血高危孕妇的一个有效手段,但也存在一些缺陷,需引起重视。Objective: To survey the carrying rate,types of gene mutation,and frequency distribution of thalassemia gene in Xiamen.Methods: Thalassemia gene detection was conducted among 3 715 patients treated in the hospital or in outpatient department of the hospital and healthy cases receiving physical examination,the results were analyzed statistically.Results: Among the cases,200 cases( 5.4%) were diagnosed as silent α-thalassemia,44 cases( 1.2%) were diagnosed as intermediate α-thalassemia,1 213 cases( 32.6%)were diagnosed as standard α-thalassemia,787 cases( 21.2%) were diagnosed as β-thalassemia,69 cases( 1.8%) were diagnosed as α-thalassemia combined with β- thalassemia.Conclusion: The detection rates of thalassemia among different objects vary,blood routine screening is an effective means for screening high-risk pregnant women,but there are some defects,which should be paid more attention to

Epidemiological survey and study of gestational diabetes mellitus

目的:调查厦门地区妊娠期糖尿病(gdM)患病率及妊娠结局,研究与gdM发病相关的高危因素。方法:收集2013年6月~2014年3月就诊于厦门市妇幼保健院的2 151例孕妇的一般资料及75 g口服葡萄糖耐量试验(OgTT)结果,并随访其妊娠结局。将调查对象分为对照组和gdM组,分析、比较两组妊娠并发症、高危因素和妊娠结局。结果:厦门地区gdM患病率为15.20%。gdM组羊水过多发生率及剖宫产率明显高于对照组,差异有统计学意义(P0.05)。年龄、孕前体重及孕前体重指数(bMI)与gdM发病率显著相关(P 0.05).Age,progestational weight and progestational body mass index( BMI) were significantly correlated with morbidity of GDM( P < 0.05).Conclusion: The pregnant women with GDM are more likely to have polyhydramnios and cesarean section.Age,progestational weight and progestational BMI are high risk factors of GDM.福建省医药卫生科技创新项目〔2014-CXB-46

RNA 干扰 mPGES-1 基因对 K562/A 细胞增殖及凋亡的影响

【目的】探讨RNA干扰膜结合型前列腺素E2合酶1（mPGES-1）对阿霉素耐药的人红白血病细胞株K562/A细胞增殖、凋亡及耐药性的影响及其可能的机制。【方法】通过RNA干扰技术抑制K562/A细胞中mPGES-1表达。分组：①未处理组（K562/A），②干扰后的阴性对照组（K562/A-NC），③干扰组（K562/A-KD），④干扰后加入外源性PGE2组（K562/A-KD+PGE2）；CCK-8法检测细胞活力；流式细胞术检测细胞凋亡；ELISA法检测PGE2浓度；Western blot检测蛋白水平。【结果】RNA干扰可明显下调K562/A细胞mPGES-1表达，抑制PGE2合成（<0.0001）。RNA干扰后，K562/A细胞增殖受抑，凋亡增加，对各化疗药物敏感性均有不同程度的增强（<0.05），同时β-catenin、MDR1的表达量减少（<0.01）。外源性PGE2可逆转RNA干扰对K562/A增殖、凋亡水平、药物敏感性的影响（<0.05），同时β-catenin、MDR1的表达上调（<0.01）；β-catenin抑制剂XAV939可浓度依赖性抑制β-catenin、MDR1蛋白的表达（<0.05）。【结论】RNA干扰mPGES-1基因可抑制K562/A细胞增殖、诱导细胞凋亡、增强细胞对化疗的敏感性，其机制与减少PGE2的合成进而下调β-catenin、MDR1的表达有关。Wnt/β-catenin通路可能参与了mPGES-1/PGE2对MDR1的调控

利用氣象資料估測尼羅草之牧草生產

In the previous investigation [Lee et al. (2004)], the aboveground fresh-based forage yield at harvest was found best-fitted to a four-variable linear regression model established from seasonal accumulated values of weather data. In this study, whether forage production during the growing periods may also be estimated by a multivariate regression model from weather data was further investigated. Both fresh and dry weights of the aboveground plant parts and plant height of nilegrass (Acroceras macrum Stapf) were measured from field experiments conducted at Taiwan Livestock Research Institute Experimental Farm (Hsinhua) during eight growing seasons from 2002 to 2004. The meteorological data were collected from on-site weather station to calculate the cumulative values along plant development during growing seasons, including the accumulated values of daily mean air temperature (DMAT), daily precipitation (DP), daily evaporation (DE), daily irradiance (IR), and daily sunshine hours (DSH). These climatic variables were used to correlate with plant height and forage production. Results showed that changes of forage production and plant height were shown linearly corresponding to the cumulative values of DMAT, DP, DE, IR, and DSH. By multiple linear regression analyses, estimations of fresh-based and dry-based forage production may be improved by two-variable linear regression models containing DP and IR, with R2=0.667 (P<0.0001) for fresh-based and R2=0.767 (P<0.0001) for dry-based forage production, respectively. Plant height may be best estimated by a two-variable model (R2=0.808, P<0.0001) containing DP and DE. When validating the models with another set of data from different growing seasons, models gave slight over-predicted values of fresh-based and dry-based forage production and plant height. Results also indicated that forage fresh weight was linearly correlated with plant height (R2=0.702, P<0.0001) and forage dry weight (R2=0.874, P<0.0001). Accordingly, unit forage production of nilegrass can be estimated by plant height at the time of sampling. 本系列研究之前項報告[Lee et al. (2004)]指 出，尼羅草(nilegrass, Acroceras macrum Stapf) 收獲時之鮮草產量可以由四氣象變因構成之直 線複迴歸模式的累積氣象數據加以合理的估測。本研究持續探討在尼羅草各生長季節期間，是否依然可由適當的多氣象變因直線複迴歸模式予以評估牧草生產量。本試驗在2002年至2004年的不同生長季節進行牧草栽培，期間分別 量測植株地上部高度(株高)、鮮重(鮮草產量)及乾重(乾草產量)，同時收集位於臺南縣新化鎮試區之農委會畜產試驗所一級農業氣象測站之氣 象測值。將各生長季節之每日氣象測值累積，即成為探討牧草生產量、株高關係之氣象變因，包括每日日均溫(DMAT)、每日降水量(DP)、每日蒸發量(DE)、每日日射量(IR)及每日日照時數(DSH)等五種。根據試驗結果，發現DMAT、DP、DE、IR 及 DSH 之個別累積測值均與牧草 生產量及株高之間呈現直線相關。再由多變因直 線複迴歸分析結果，顯示對鮮草及乾草生產量之估測，可藉由DP及IR兩變因累積值構成之二變因直線複迴歸模式提高估測準確度，鮮草及乾草的決定係數分別達R2=0.667 (P<0.0001)及R2=0.767 (P<0.0001)。對株高之估測，則可藉由DP及DE兩變因累積值構成之二變因直線複迴歸模式提高估測準確度，決定係數為 R2=0.808 (P<0.0001)。經以另一組生長季節氣象資料驗證模式對其他氣象環境之適用性，發現預測值均略為高於實測值。試驗結果又顯示，鮮草生產量與乾草生產量及株高之間均呈現直線相關，顯然吾人可以在生長期間取樣調查株高，再以之推估尼羅草之單位牧草生產量

Short-term Soil Water Deficits Occurring in Vegetative Phase to Growth and Development of Asiatic and Oriental Hybrid Lilies

本研究係於臺灣省農業試驗所農場進行田間試驗，以兩類不同商用雜交型百合-亞洲型百合’Elite’和東方型百合’Suir gazer’爲試材，選擇雜交百合適宜生長的12月定植。在開花前予以缺水處理，使其土壤水分潛勢降至-0.20 MPa和-0.25 MPa兩種等級，探討短期缺水對進口百合營養生長、生殖生長及回收子鱗莖周徑和鲜重之影響。試驗結果顯示，’Elite’和’Star gazer’的植株高度和葉面稻隨著土壊水分潛勢降低而有減少的趨勢，在株高表現上，’Elite’較‘Star gazer’對缺水敏感，而葉面桢則反之。當土壤水分潛勢降至 -0.25 MPa時，’Elke’的單株花朵數減少1〜2朵，開花日數亦縮短3天，因此短期缺水將改變’Elite’之開花特性，’Star gazer’則未明顯變化。’Elite’在土壤水分潛勢達-0.25 MPa時所回收之子鳞莖周徑和鮮重，皆低於對照植株，顯示土壤缺水影響其鳞片充實的能力。 Bulbs of Asiatic hybrid lily 'Elite' and Oriental hybrid lily 'Star gazer’ were planted in the experimental field at Taiwan Agricultural Research Institute (120° 42’ E, 23° 30' N, elevation of 85 m) to study short-term soil water deficits occurred in the vegetative phase on plant growth, flowering, and daughter bulbs formation. Results indicated that plant height and leaf area were reduced by water deficits in both types of lily. The decrease of plant height in ’Elite’ was greater than that of 'Star gazer', and vice versa in the result of leaf area. The number of flower per plant and flowering duration in ’Elite’，but not in 'Star gazer’，were reduced when soil water potential lowered down to -0.25 MPa. Additionally, bulb circumference and fresh weight in 'Elite' were also decreased by soil water defict at -0.25 MPa

Studies on Seedling Productive Environment and Management Techniques of Strawberry

台灣草莓種苗生產環境通常尋求位在冷涼環境露天栽培，但露天栽培並無法完全克服因夏季高溫多濕及強降雨所誘發病蟲害之問題，鑑此，尋求通風良好且具防雨之設施，以供種苗經濟栽培所需，實為必要解決的問題。近年來草莓產業因受全球氣候變遷影響，台灣利用溫網室栽培草莓面積逐漸增加，並配合應用自動化環境控制及節能光電輔助系統而建置。本試驗研究於農業試驗所現有半封閉式開頂溫室為種苗量產之基礎運作平台，以溫室正壓送風暨環境隔離系統之技術、水質淨化處理和介質滅菌處理，利用無病毒之組培苗 (原原種)，並利用養液供水降溫維持體系，可將草莓生長的根溫控在28–31℃，再結合低農藥與非農藥資材之病蟲害監測管理等技術之整合與運用，期能建立草莓健康種苗生產環境整合管理體系，供草莓種苗產業之利用。由本試驗設施之種苗管理整合技術生產的每株草莓苗株數平均為50 株，相較於農民種植的35 株提高了35-40%，在高效隔離環境整合管理技術—草莓健康種苗生產標準作業程序技術下，每公頃可生產健康種苗25–28 萬株，年產值約為375–420 萬元。 The seedling production environments are usually cultivated in the cold climate and unsheltered field of strawberry in Taiwan, it is hard to overcome the problem of plant diseases and insect pests induced by higher temperature, humidity and rainy days. It is important to seek one protected facilities for seedling production of strawberry. To cultivate strawberry with greenhouse has increased in Taiwan by global climate change in recent years, and the application automation environment control and energy saving photoelectric systems were build. This project is to stabilize the plastic semi-enclosed greenhouse production system. We intend to integrate and develop technologies of cultivating strawberry in plastic greenhouse by application vertical culture system and hydroponic method about 28–31℃ (peat moss medium). We are also to maintain medium temperature, controlling air exchange, set up air clean filter and tougher prevention of pests and diseases. This technology provides healthy strawberry seedling cultivation and production of standard operating procedures, can produce 50 plant per mother plant higher than farmer, healthy seedlings 25–28 Million plants per hectare, the annual output value is about 375–420 Million dollars per hectare

鱗莖周徑及切花處理與亞洲型百合生長、開花及繁殖之關係

Potted experiments were conducted at Experimental Farm of Taiwan Agricultural Research Institute to evaluate bulb size and cut flower effects on growth, flowering, and propagation of Asiatic hybrid lilies `Elite' and `Jolanda' in 1994 and 1995.本研究係於臺灣省農業試驗所農場進行盆栽試驗，1994年試驗種植進口亞洲型百 合 `Elite' 和 `Jolanda' 周徑 12-13cm 的鱗莖，1995 年試驗種植不同周徑等級的鱗莖， 以探討鱗莖周徑及切花處理與亞洲型百合 `Elite' 和 `Jolanda' 生長、開花及繁殖之關係 。 試驗結果顯示，`Elite' 和 `Jolanda' 和莖高、節位數和葉面積皆隨鱗莖周徑之增大而 增加，呈正相關關係； `Elite' 和 `Jolanda' 植體的莖周徑隨鱗莖周徑增大而加粗，相對