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京都大学0048新制・課程博士工学博士甲第1959号工博第527号新制||工||385(附属図書館)5291UT51-52-N24京都大学大学院工学研究科資源工学専攻(主査)教授 平松 良雄, 教授 伊藤 一郎, 教授 港 種雄学位規則第5条第1項該当Kyoto UniversityDA

Application of Electrochemical Techniques to the Researches in Life Science

本文简要介绍作者近两年来采用电化学方法并结合其他现代生物、化学技术 ,在生命科学几个热点领域所开展的一些工作 ,主要包括 :蛋白质的功能转换、生物信号分子及其与蛋白质的相互作用、生物分子的电子传递、基因检测、结构生物学、生化分析与第三代生物传感器等几个方面The research work of our group in the last two years is briefly reviewed in this paper. By using electrochemical method, and combining some other chemical and biological techniques, lots of achievements have been obtained. And these researches are towards some hot topics in life science, such as function conversion, interaction between signal molecules and proteins, electron_transfer of biomolecules, gene detection, structural biology, biochemical analysis and the third generation biosensors.作者联系地址：南京大学生物化学系医药生物技术国家重点实验室,南京大学生物化学系医药生物技术国家重点实验室,南京大学生物化学系医药生物技术国家重点实验室 江苏南京210093 ,江苏南京210093 ,江苏南京210093Author's Address: Dept. of Biochem. and National Key Lab. of Pharmaceutical Biotechnology, Nanjing Univ., Nanjing 210093,Chin

Electrochemical Study of Protein Modification

蛋白质的翻译后修饰对于生命体执行正常的生理功能具有十分重要的作用，是蛋白质科学研究的重要内容.目前研究蛋白质修饰的方法主要有质谱法、亲和层析等，然而由于蛋白质修饰研究的复杂性，迫切需要发掘新的技术手段.电化学方法理论成熟、应用广泛，在生命科学许多领域发挥着越来越重要的作用.蛋白质的体外修饰必将导致蛋白质特定位点基团的变化，可以利用巧妙设计的电化学方法予以表征和分析，以期探明修饰对蛋白质结构和功能的影响.此外，又可以利用电化学定量分析的独特优势快速准确地测定蛋白质修饰中涉及的相关酶活.正因为如此，蛋白质体外修饰的电化学研究已引起越来越多的关注.本文以作者课题组近期研究工作为主，结合国内外同行的相关代表性工作，介绍电化学方法在蛋白质修饰方面的近期研究进展，并探讨了今后的发展方向和趋势.Protein post-translational modification is a key physiological procedure, which has received more and more research interests from the scientists not only in protein science but also in some other fields. The usually employed techniques for the study of protein modification include mass spectrum, affinity chromatography, etc. Considering the complexity of protein modification and the urgent demand of such kind of study, it is highly required to develop new techniques to perform more studies. Electrochemical methods, which have been widely used in chemical study and analysis, are playing an increasingly important role in many fields of biological studies. The modification of protein may induce chemical changes of specific groups, which may be characterized by using electrochemical methods via various approaches. Therefore, the changes of protein structure and function through protein modification can be revealed and the enzyme activity involved in protein modification may be detected rapidly, sensitively and accurately by taking the advantages of electrochemical methods, and consequently more and more achievements have been obtained. Based on some recent studies in the group of the authors and also some interesting works of other groups, this article reviews the progress of electrochemical study on protein modification, so as to exchange the research ideas with the colleagues in electrochemical community and some others related to protein science.国家自然科学基金资助项目（90406005, 20575028）和国家杰出青年科学基金资助项目（20925520）资助作者联系地址：1.上海大学生命科学学院，上海 200444；2.南京大学生物化学系，医药生物技术国家重点实验室，江苏 南京 210093Author's Address: 1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; 2. Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China通讯作者E-mail：[email protected]

Lithium Intercalation Performance of P-Doped Phenolic Resin Pyrolysis Carbon

以添加不同含量五氧化二磷的热塑性酚醛树脂为前驱体 ,经热固化后升温至 70 0℃裂解制备掺磷树脂裂解炭 .X射线衍射考察了裂解炭的微晶结构变化 ;恒电流技术研究了裂解炭的充放电性能 .实验表明 ,五氧化二磷的加入使树脂裂解炭微晶结构发生了很大改变 ,随着磷含量的增加微晶层间距减小 ,而且更加无序 ;其放电可逆容量随五氧化二磷掺杂量的增加先增而后减 ,当P2 O5含量为 9wt%时 ,可逆容量达到最大值 (5 2 8mA·h·g- 1) ,是未掺杂 (2 30mA·h·g- 1)的 2倍多Phosphorus-doped phenolic resin carbon was prepared by low temperature(700 ℃)pyrolysis of thermoplastic phenolic resin containing different amounts of phosphorus pentoxide after curing. X-ray diffraction pattern and the constang-current charge-discharge properties of phenolic resin carbon were studied. The results showed that the phenolic resin carbon microstructure became out of order with increasing of content phosphorus pentoxide,otherwise,the first discharge reversible capacities increased firstly and then decreased. The first discharge reversible capacity of phenolic resin carbon containing 9wt% phosphorus pentoxide was 528 mA·h·g -1 which was higher than that of non-phosphorus doped phenolic resin carbon(230 mA·h·g -1 ) did.作者联系地址：中国科学院山西煤炭化学研究所煤转化国家重点实验室,中国科学院山西煤炭化学研究所煤转化国家重点实验室,中国科学院山西煤炭化学研究所煤转化国家重点实验室,中国科学院山西煤炭化学研究所煤转化国家重点实验室,中国科学院山西煤炭化学研究所煤转化国家重点实验室,中国科学院山西煤炭化学研究所煤转化国家重点实验室 山西太原030001 ,山西太原030001 ,山西太原030001 ,山西太原030001 ,山西太原030001 ,山西太原030001Author's Address: State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001,Chin