A Brief Analysis of Diversif ication of Public Ownership in Current Period

摘　要:从内因和外因两个方面论述了公有制实现形式多样化的原因,其内因就是公有制本身的内部矛盾,内部矛盾潜藏了多种实现形式的需要。其外因就是不同的交易特征结构及制度变迁中的利益冲突。不同的交易特征要求有不同的产权实现形式与之相适应,而制度变迁中的各利益集团之间的政治谈判力的对比又使产权实现形式更加不确定。 Abstract :This paper discusses the causes of diversification of public ownership from the two points of internal and external . The internal cause is just the internal contradiction in the public ownership itself , which hides the need of diversification , while the external cause is just the beneficial conflict in the different transaction characteristics structures and in the institutional changes. Different transaction characteristics require different forms of property rights , and in the institutional changes the differentials of political negotiation abilities among every beneficial group have also made the forms of property rights more indefinite

Preparation and identification of polyclonal antibody raised against heat shock protein 90 of fission yeast

作者简介:李文珠(1983－),女,博士研究生,主要研究方向:细胞生物学 通信联系人：靳全文，教授，主要研究方向：细胞周期调控及表观遗传学， [email protected][中文文摘]为制备兔抗裂殖酵母Hsp90多克隆抗体,在通过PCR获得了裂殖酵母Hsp90(Swo1)基因后,构建了pMALc2x-Swo1表达载体,可用于表达编码正确氨基酸序列的目的基因。转化大肠杆菌BL21(DE3),IPTG诱导表达,Amylose Resin柱纯化。目的蛋白表达量占菌体总蛋白的30%以上。纯化后,蛋白纯度达95%以上。纯化后的MBP-Swo1融合蛋白抗原加福氏完全佐剂背部皮内注射首次免疫新西兰大白兔,第28天用MBP-Swo1融合抗原加福氏不完全佐剂同样剂量加强免疫,第35天时再次免疫。第49天心脏采血。收集血清后,用免疫印迹(westernblot)检测Swo1多克隆抗体的特异性。免疫印迹检测结果显示该抗体能够特异性识别内源性的裂殖酵母Hsp90/Swo1蛋白,但并不识别人类细胞中的Hsp90α/β。[英文文摘]To prepare the polyclonal antibody of Hsp90(Swo1) of fission yeast Schizosaccharomyces pombe,the encoding gene swo1+was amplified from the yeast genome and then inserted into expression vector pMALc2x.The resulted plasmid pMALc2x-Swo1 was transformed and expressed in E.coli BL21(DE3).The expressed fusion protein was purified through Amylose Resin column.The proteins were expressed mainly as secretion with the yield of more than 30% of total bacterial proteins.After purification,the purity of the proteins was about 95%. The New Zealand white rabbits were immunized with Freund’s complete adjuvant plus purified MBP-Swo1 fusion antigen through the back skin intradermal injection for the first time. The same dose of Freund’s incomplete adjuvant plus MBP-Swo1 was injected to strengthen the immunity after 28 and the 35 days respectively. After 49 days, blood sample was collected from heart, and antisera were extracted. The specificity of Swo1 polyclonal antibody was examined by Western blot. It showed that the antibody obtained had a high specificity to detect endogeneous Swo1 from fission yeast, but it could not recognize Hsp90α/β in human cells. Key words: fission yeast；Hsp90/Swo1；polyclonal antibody.高等学校博士学科点专项科研基金资助项目(20100121110003);国家自然科学基金资助项目(30871376);教育部科学技术研究重点项目(108076

Function of heat shock protein 90 (Hsp90) in heterochromatic gene silencing in fission yeast

鉴于高等生物中HSP90与ArgOnAuTE蛋白的功能相关性,研究了裂殖酵母中HSP90/SWO1与AgO1蛋白之间的相互关系以及对异染色质区基因沉默的影响。结果表明,裂殖酵母中SWO1蛋白通过与AgO1蛋白的相互作用,可以稳定AgO1蛋白,并且这种相互作用依赖于SWO1的n端和中央结构域以及AgO1的n端和PAz结构域。在着丝粒的OTr区和IMr区,SWO1+基因的突变会引起区域内基因沉默的解除,并且与rnAI组分双突变后(AgO1Δ或dCr1Δ),基因沉默解除的效果加强。在交配型区,SWO1+基因突变后也会引起显著的基因沉默解除现象。当SWO1+基因突变后,依赖于TAS3的人工异染色质区基因沉默解除。研究发现了裂殖酵母中热激蛋白HSP90的新功能,即参与异染色质区的基因沉默调控。Given that Hsp90 functionally correlates with Argonaute in higher eukaryotes,we examined the interaction between Hsp90(Swo1) and Ago1and the function of Hsp90 on heterochromatic gene silencing in fission yeast Schizosaccharomyces pombe.The results showed that Swo1 could stabilize Ago1 through interaction with Ago1 in fission yeast.The interaction between Swo1 and Ago1 depends on N domain and the central region of Swo1,as well as the N and PAZ domains of Ago1.The centromeric silencing was alleviated in the swo1-26 mutant,but the silencing of reporter gene at telomere and rDNA regions remained unchanged.Double mutants of swo1-26 dcr1Δ and swo1-26 ago1Δ showed enhanced silencing defects at centromeric regions.In the mating type region,we observed a significant derepression of gene silencing in the swo1-26 mutant.The artificial RITS complex-dependent silencing system was also defective in the swo1-26 mutant.Our results found a new role of Hsp90 in gene silencing at heterochromatin regions in S.pombe.高等学校博士学科点专项科研基金资助项目(20100121110003);国家自然科学基金面上项目(30871376);教育部科学技术研究重点项目(108076

不同温度无水乙醇消融兔VX2移植性肝癌的实验研究

【目的】探讨不同温度无水乙醇瘤内注射对兔VX2移植性肝癌消融治疗效果。【材料与方法】32只新西兰大白兔肝内植入瘤块，随机分为25℃、50℃、60℃、70℃4组，各8只，在CT导向下经皮穿刺向瘤内注入不同温度的无水乙醇各1mL，CT扫描测定碘油范围，一周后取肿瘤行病理学检查，观察相同剂量不同温度无水乙醇对肿瘤组织所致凝固坏死的范围。【结果】肿瘤坏死面积随温度增加而扩大，50℃组与常温组差异无统计学意义，60℃和70℃组与常温组差异有统计学意义，60℃组和70℃组差异有统计学意义（P均〈0．0）。【结论】加热至60℃以上的无水乙醇对兔VX2移植性肝癌的消融效果较常温好。是一种不增加无水乙醇用量又能达到扩大消融范围的肝癌局部消融方法

聚苯乙烯接枝聚甲基丙烯酸甲酯共聚物的合成新方法

聚苯乙烯接枝聚甲基丙烯酸甲酯共聚物的合成新方法①郭金全邹友思戴李宗袁亿文潘容华（厦门大学化学系厦门３６１００５）阴离子聚合［１］和基团转移聚合（ＧＴＰ）［２］技术可分别使非极性单体和极性单体进行活性聚合，得到分子量分布窄、实际分子量和设计分子量相近的..

一种高效、稳定的分泌型原核表达载体的构建及应用

以本室构建的原核表达载体pTO-T7为基础载体,PCR合成ompT引导序列,插入该载体多克隆位点上游,构建了分泌型原核表达载体pTO-OT。将2个外源基因克隆至pTO-OT,2个重组质粒在大肠杆菌中均得以高效表达,表达量为25％-30％。Western印迹分析证实了重组蛋白在大肠杆菌中表达后可被信号肽酶有效识别,切割后的重组蛋白具有良好的免疫学活性。对重组表达菌株的连续传代实验证实了该表达载体具有良好的遗传稳定性,显示了该原核表达载体在基因工程中的应用价值

一种高效、稳定的分泌型原核表达载体的构建及应用

以本室构建的原核表达载体pTO-T7为基础载体,PCR合成ompT引导序列,插入该载体多克隆位点上游,构建了分泌型原核表达载体pTO-OT。将2个外源基因克隆至pTO-OT,2个重组质粒在大肠杆菌中均得以高效表达,表达产量在25％～34％之间。Western blot分析证实了融合蛋白可被大肠杆菌信号肽酶有效地切割,并具有良好的免疫学活性。对重组表达菌株的连续传代实验证实了该表达载体具有良好的表达稳定性,显示了其在基因工程中的应用价值

抗HEV嵌合抗体的构建及在CHO细胞中的表达

通过RT-PCR方法从分泌戊型肝炎(戊肝)病毒中和性鼠源单克隆抗体(单抗)8C11的杂交瘤细胞中克隆出抗体基因的重链可变区(VH)、轻链可变区(VK)序列,并分别克隆到含有人gamma1重链和kappa轻链恒定区序列的pcDNA3 1/Hygro和pcDNA3 1(+)质粒中,共转染中华仓鼠卵巢癌细胞(CHO)细胞。RT-PCR结果表明,转染的CHO细胞转录了嵌合重链及轻链基因,间接ELISA及Westernblot结果表明:翻译出的两种多肽在细胞内正确组装成嵌合抗体分子,并可分泌至细胞外,表达的嵌合抗体保留了原鼠单抗的抗原结合特异性及对8H3结合抗原的增强作用。8C11嵌合抗体的成功表达可降低鼠源性,为探讨戊肝抗体治疗的可能性奠定了基础

Ni/SiO2在甲烷部分氧化反应中的稳定性:W修饰的影响

甲烷部分氧化制合成气反应(POM)是天然气、页岩气资源利用的重要途径之一,常用的Ni/SiO2催化剂在反应中易发生表面积炭而失活。为了解决这一问题,我们采用尿素沉淀法制备W修饰的Ni基催化剂,并考察其在POM反应中的稳定性和W的作用。结果表明,催化剂中适量W的存在可显著改善其POM反应稳定性。其原因为Ni-W作用修饰了Ni的化学态或其亲氧能力,从而改善了其表面抗积炭能力。此外,反应中催化剂表面形成的α-WC具有一定的抑制表面积炭形成的能力,且该α-WC具有良好的稳定性。国家自然科学基金(21373169);;\n教育部创新团队(IRT1036)资助项目~