改良分子信标-双重实时荧光PCR快速检测SARS病毒

目的建立改良分子信标-双重实时荧光PCR检测SARS病毒的方法,用于SARS的早期诊断和动物溯源。方法利用改良分子信标技术、装甲RNA和双片段双色荧光技术,根据GenBank公布的SARS病毒聚合酶基因1b的阅读开放框架结构的保守序列,自行设计一对引物和探针,以部分临床标本的酶联吸附实验结果和传统细胞培养方法作为对照,建立分子信标检测SARS病毒的方法。对368份临床标本(咽漱液、血液、粪便、尿液)、52份细胞培养液和50份动物标本进行荧光PCR扩增。结果分子信标检测SARS病毒的方法灵敏度为10～100个拷贝ml,与流感病毒等呼吸道病毒无交叉反应。分子信标检测368份临床标本,20份阳性。其中确诊病例阳性率为21.27%(1047),确诊病例的咽漱液阳性率为43.48%,还分别从粪便和血清中检测到SARS病毒。52份细胞培养液,29份阳性,阳性率为55.77%。50份动物标本,23份阳性,阳性率为46%。结论改良分子信标-双重实时荧光PCR检测SARS病毒方法灵敏度高、特异性强,可用于SARS的临床早期诊断和动物溯源

钙化合物的种类对脱氯特性的影响

以PVC为HCl的释放源，采用热重法对不同气氛下各种钙化合物的脱氯特性进行了研究，探讨了各种钙化物的脱氯机理以及脱氯效率。结果表明和PVC作为氯化氢的释放源是可行的。3种钙化物中氢氧化钙的脱氯效果最好。氧化钙与氢氧化钙的脱氯机理基本相似。各种钙化物在不同气氛下的脱氯机理是不同的。燃烧状态下，在高温段钙化物脱氯产物氯化钙有分解迹象

The Concept Change and Organization Construction of Faculty Development in China

理念决定着大学教师发展实践探索与组织建设的方向。我国大学教师发展理念正在经历三重转变:一是性质上由补偿性发展向提升性发展过渡,二是内容上由泛指的教师一般发展向教师教学发展深化,三是组织上从分散的行政管理部门职能向专业的学术服务机构转型。基于教师发展的现状及新理念的萌芽,加强大学教师发展的组织建设成为回应大学教师发展理念转变的当务之急。我国大学教师发展组织需通过推进教师发展组织自身的专业化、实现教师发展组织建设的系统化、提升教师发展组织建设的科学化等三条路径来进行有针对性的变革,以实现组织建设与创新。The practice exploration and organization construction of faculty development is guided by its concept.The concept of faculty development in China is going through three shifts: the first is from compensatory development to promotion development in nature, the second is from focusing on general development to teaching development, and the third is transforming from scattered administrative management functions to professional academic service organization.Based on the current situation of faculty development, strengthening the organization construction becomes urgent in response to the concept changes.In order to achieve the organization construction and innovation objectives, the faculty development organization in China should promote its own professionalization, systematize its organization construction, and make it more scientific.教育部人文社会科学重点研究基地2014年度重大项目“大学教师发展的理念、内涵、方式与动力”(项目编号:14JJD88003

Interplay of Nanoparticle Properties during Endocytosis

Nanoparticles (NPs) have been widely applied as drug carriers in drug delivery, due to their unique physical and structural properties. To achieve the drug delivery purpose, receptor-mediated endocytosis is a primary explored mechanism to internalize NPs into tumor cells. During the endocytosis process, properties of NPs, including size, shape, and surface functionality, play an important role in determining the final drug delivery efficacy. Many of these NP properties have been extensively explored individually. However, the multiple NP properties naturally interplay with each other in the endocytosis process to determine the internalization efficiency together. Therefore, it is significantly important to understand the interplay of different NP properties to improve the NP's final delivery efficacy. In this review, we focus on the interplay of NPs properties on the endocytosis process to summarize the relevant experimental observations and physical mechanisms. Particularly, three different aspects are discussed in detail, including the interplay between size and shape; size and elasticity; shape and elasticity. We have summarized the most recent works and highlighted that building up systematic understandings for the complex interplay between NP properties can greatly help a better design of NP platforms for drug delivery

胞质分裂过程中的力学因素对细胞分裂速度的调控研究

目的细胞进行胞质分裂时,位于细胞赤道区域的收缩环产生的收缩力使细胞质膜内陷形成卵裂沟将细胞一分为二。胞质分裂过程是一个高度依赖于力学作用的过程,在胞质分裂过程中收缩环的收缩力、细胞赤道区域之外的两极皮层骨架产生的力以及细胞的内压都对细胞胞质分裂过程具有重要影响。胞质分裂过程中的力学因素对于胞质分裂速度的影响目前尚不明确

脂筏对细胞黏附蛋白键合作用的影响

目的细胞黏附通过受体、配体蛋白的键合作用介导实现,其调控着信号转导、组织成形、癌症转移、免疫应答等诸多关键的细胞生命活动。深刻认识受体与配体的键合作用对理解细胞通讯、促进药物研发具有重要的理论和现实意义。脂筏作为细胞膜上富含胆固醇与鞘磷脂的信号转导平台,通过募集蛋白质调控着大量的细胞生物学功能。目前关于脂筏对细胞黏附中受体-配体键合作用的影响研究仍处于探索阶段,其具体的调控机制尚不明确。方法 基于统计力学理论与Monte Carlo计算机模拟方法,针对细胞-细胞黏附与细胞-基质黏附两种典型的细胞黏

Research on Controlling Mobile Robots Using GPRS System

以移动探察机器人为平台,介绍了一种利用GPRS通讯系统控制机器人的新方法.重点介绍了对GPRS通信进行端到端加密的改进方法,该方法在ELGam al公开密钥算法中加入时间标志,增强了系统的安全性.最后,给出了实验结果

Imitating trunk flexible mechanism

本装置属于机器人连接机构,其结构是由多节并联机构1、2、3串联而成。而每节并联机构由三个分支杆、上下两平台连接及铰链组成。由多节这样的并联机构串联起来,就构成了具有超多自由度的柔性臂机构。由于本机构能实现转动与移动的复合运动,因此具有较高的柔性避障钻洞能力。且还具有高的刚度和精度。</p