Quantitative Analysis Model for Fiber-Optic Chemical Sensors Based on Fluorescence Quenching for Analytes and Its Application

根据光纤化学传感器的结构与信号传输特征 ,推导并建立了适合于定量描述基于荧光猝灭原理的光纤化学传感器对待测物响应的非线性数学模型 .以此为基础 ,为更加简捷、直观地反映响应信号与待测物浓度的函数关系 ,建立可线性化的回归方程 ,准确预报待测物的浓度 ,提出了多模型回归技术建立此类光纤化学传感器定量分析模型的方法 .为验证定量分析模型的适用性 ,将该法应用于芘丁酸光纤化学传感器对甲硝唑、呋喃妥因和氧氟沙星等待测物响应的最佳数学模型的选择之中 .结果表明 ,所选的数学模型对各待测物在一定范围内的浓度都能进行准确的预报Because the fiber-optic chemical sensors based on the fluorescence quenching has an adjustable short distance from the membrane to the end of fiber optics, forming a space of microcell, a diminution of fluorescence is induced by inner filter effects, involving absorption of both excited light from the light source and emitted light from the membrane. If the absorption spectra of analytes overlap the fluorescence excitation and/or emission spectra of the fluoroprobe in the membrane, the quenching signal of the fiber-optic chemical sensors would be produced by the resonance energy transfer. In addition, dynamic quenching happens to some haloid, heavy metal compounds and aromatic nitro organic compounds. According to the structural characterization of the fiber-optic chemical sensors based on fluorescence quenching and the mechanism of fluorescence multiple quenching, a non-linear mathematical model was deduced and described for the quantitative analysis model for the fiber-optic chemical sensors. A multiple model regression technique for the quantitative analysis model was reported to provide rapidly and directly the relations between the response signal and the concentration of analytes and establish linear regression equation for predicting the concentration of analytes. The technique was applied to fit best mathematical model from the control samples of the therapeutic drugs based on the response of fiber-optic chemical sensors, such as metronidazole, nitrofurantoin and ofloxacin. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the fiber-optic chemical sensor to response the samples. The experimental results showed that these models had some good characteristics and gave an alternative method for establishing quantitative analysis models for the fiber-optic chemical sensors.国家自然科学基金 ( 2 9775 0 2 2 )资助项

谷氨酸棒杆菌合成生物絮凝剂分批发酵过程的动力学模拟

对谷氨酸棒杆菌生产生物絮凝剂的分批发酵过程进行了动力学研究。以Logistic方程来描述菌体生长过程,并对Gaden生长相关模型进行了修正,加入时间修正因子,建立了产物生成动力学模型,同时还提出了关于葡萄糖和尿素的两个非线性基质消耗动力学模型。利用软件Origin8.0对实验数据进行拟合,结果显示各模型均能较好地描述谷氨酸棒杆菌合成生物絮凝剂的分批发酵过程

Quantitative analysis model for fiber-optic chemical sensors based on fluorescence quenching for analytes and its application

Because the fiber - optic chemical sensors based on the fluorescence quenching has an adjustable short distance from the membrane to the end of fiber optics, forming a space of microcell, a diminution of fluorescence is induced by inner filter effects, involving absorption of both excited light from the light source and emitted light from the membrane. If the absorption spectra of analytes overlap the fluorescence excitation and/or emission spectra of the fluoroprobe in the membrane, the quenching signal of the fiber - optic chemical sensors would be produced by the resonance energy transfer. In addition, dynamic quenching happens to some haloid, heavy metal compounds and aromatic nitro organic compounds. According to the structural characterization of the filer - optic chemical sensors based on fluorescence quenching and the mechanism of fluorescence multiple quenching, a non - linear mathematical model was. deduced and described for the quantitative analysis model for the fiber - optic chemical sensors. A multiple model regression technique for the quantitative analysis model was reported to provide rapidly and directly the relations between the response signal and the concentration of analytes and establish linear regression equation for predicting the concentration of analytes. The technique was applied to fit best mathematical model from the control samples of the therapeutic drugs based on the response of fiber - optic chemical sensors, such as metronidazole, nitrofurantoin and ofloxacin. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the filer - optic chemical sensor to response the samples. The experimental results showed that these models had some good characteristics and gave an alternative method for establishing quantitative analysis models for the filer - optic chemical sensors

The Japanese Watakushi shosetsu/Shi shosetsu as Seen in Relation to Asian Culture : Comparison with Asian Language and English Translations as Opportunity

研究成果の概要 (和文) : 2年間の研究実施期間においては、主にワークショップと研究集会を開催することによって研究をすすめた。18年度には夏・冬にワークショップ、秋に研究集会と合計8回、19年度には夏にワークショップ、春・冬に研究集会と合計6回、あわせて14回の勉強会を開催した。これらの内容報告については、本研究課題ウェブサイト(http://www.i-novel-hosei.org/kaken.htm)にて公開している。西欧ではなくアジア文化との比較から日本の「私小説」を考えるという目的から出発した本研究は、研究実施期間において次の5つの研究を行った。1つめは、中国、韓国、台湾における「私小説」の認知状況のアンケート調査である。これらの結果からは認知を越えて、各地域での「私小説」に関する認識を得ることができた。2つめは、アンケート結果から示唆を得た各地域における、日本近代の「私小説」に影響を受けた作品、「私小説」に類似する現代の個人化傾向のある作品についての具体的検討である。3つめとして、これら各地域における「私小説」的作品の質的な差違が、歴史や文化的背景によるものであることを検討し確認した。4つめは、志賀直哉「城の崎にて」の中国語訳、韓国語訳との翻訳比較である。5つめは、海外の「私小説」研究書として、魏大海『私小説 20世紀日本文学的一个"神話"』(中国)、安英姫『日本の私小説』(韓国)の全訳の完成、E・ファウラー『告白のレトリック-20世紀初期の日本の私小説(米国)の「序論 私小説における現象と表象」の翻訳を行った。これらの成果は「研究成果報告書」に収めている。研究成果の概要 (英文) : We have made progress by holding workshops and research meetings in the 2 years of research period. For example, we had 8 meetings including the summer and winter workshops as well as research meetings in autumn in 2006. In 2007, 6 meetings were held including 1 summer workshop and 5 research meetings in spring and winter. As a result, a total of 14 meetings were held during our research period. The outcomes of our research are disclosed on the dedicated website of this research program (http://www.i-novel-hosei.org/kaken.htm). The research program features a Watakushi shosetsu/Shi shosetsu comparison between Japanese works and Asian works instead of Japanese-Western comparison. It is composed of 5 sub researches: 1) The 1st sub research was an investigation on the cognition of Watakushi shosetsu/Shi shosetsu in China, Korea and Taiwan. We caught a picture of how Watakushi shosetsu/Shi shosetsu are recognized in these regions through this initial sub research. 2) In the 2nd sub researc h, we chose several locals works which are identified as being influenced by or considerably close to the modern Watakushi shosetsu/Shi shosetsu in Japanese, and discussed these works in details. 3) We discussed and formulated our conclusion on reasons of the substantial differences among these Asian works, which we believe were developed from their different historical and cultural background. 4) We made a comparison between the Chinese translated version and the Korean version of Shiga Naoya\u27s “Kinosaki nite" (“At Kinosaki"). 5) We translated several overseas works into Japanese. Those works include Wei Da hai\u27s “The Watakushi shosetsu/Shi shosetsu-a miracle of 20th Century\u27s Japanese Literature" (China), An Young hee\u27s “The Japanese Watakushi shosetsu/Shi shosetsu “(Korea), Edward Fowler\u27s “The Rhetoric of Confession-the early 20th Century\u27s Japanese Watakushi shosetsu/Shi shosetsu-Introduction phenomenon and symbols of Watakushi shosetsu/Shi shosetsu" (US). All the outcomes of our research mentioned above are presented in our “Research Report"

ゴケイセイ ノ フ ノ レンサ フコウセイ ナ アツカイ ヲ ウケル ト ダイサンシャ ニモ フコウセイ ナ アツカイ ヲ スル ノカ

論文Research Articleヒトは相互協力を達成することで、他の種よりも大きな集団を形成し、それが国や文化といったさまざまなものを形成してきた。つまり、相互協力を達成することはヒト社会を未来につなげるための重要な要因の1 つであると考えられる。本研究では、非協力的な扱いを受けたとき、自分が別の人物に対して同じように非協力的な行動をとること、すなわち不公正の連鎖がどのような要因によって生じるのかを検討した。先輩から押し付けられた面倒な仕事を後輩または同期に押し付けるというシナリオで実験を行い、相手が後輩であるときに不公正の連鎖が生じ、相手が同期であるときには生じないと予測された。また、評価懸念の高さが後輩への押し付けを抑制すると予測された。実験の結果、相手が後輩か同期かに関わらず不公正の連鎖が生じ、評価懸念の高さは不公正の連鎖を抑制しておらず、仮説は支持されなかった。その理由として、同期と後輩に対して別の認知過程によって不公正の連鎖が生じたことや、本研究の方法が適切ではなかった可能性があり、さらなる検討が必要である。In this study, we examined what factors cause a person to behave in an uncooperative manner toward another person when he or she is treated uncooperatively, i.e., what factors cause a chain of unfairness. Two experiments were conducted using the following scenario; the participants were instructed to impose a troublesome task which was imposed by a senior colleague on a junior colleague or a peer. It was hypothesized that a chain of unfairness would occur when the other party was a junior colleague and not when the other party was a peer. It was also hypothesized that higher Fear of Negative Evaluation (FNE) would suppress the imposition on juniors. Contrary to the hypotheses, the results showed that a chain of unfairness occurred regardless of whether the partner was a junior colleague or a peer, and higher FNE did not inhibit the chain of unfairness. The reasons for this may include the possibility that a chain of unfairness was caused by a different cognitive process for peers and juniors and that the method of this study was not appropriate. Further study is needed

麝鼠生态养殖模式的初探

通过长期的、多种方式的麝鼠饲养实践,借鉴农业生态理论,总结出6种麝鼠生态养殖模型:1.麝鼠——沼泽地结合型2.麝鼠——鱼塘结合型3.麝鼠——稻田结合型4.麝鼠——园、林结合型5.麝鼠——庭院结合型6.综合利用型这些模型给出了许多种麝鼠饲养的方式,可以充分利用合适的空间,提高能量及废物利用率,具有很高的经济效益和社会效益

A Multivariate Linear Model for Calculating Response Feature Parameters of Chemical Sensor Based on Fluorescence Quenching for Analytes

根据基于荧光猝灭原理的化学传感膜的结构特征和发光机理 ,推导并提出量化作用于膜传感器的多种荧光猝灭因素的多元线性模型 .结合三杯法 ,该模型可简便地求出反映不同荧光猝灭机理的特征参数 .通过建立的数学模型 ,测定并计算了芘丁酸膜传感器对应于呋喃妥因等 2 3种药物多种荧光猝灭因素的响应数据和响应参数 .结果表明 ,传感膜对分析物的响应特征往往能得到主要猝灭因素响应模型的近似反映 ,这为基于荧光猝灭原理的化学传感器定量分析模型的建立提供了可借鉴的思路和方法A mathematical model was deduced and described for the different mechanism of fluorescence quenching to analytes based on the essential quenching models including the dynamic quenching, the resonance energy transfer and the inner filter effects involving absorption of both excited light from the light source and emitted light from membrane. The model reported here indicated that the logarithmic functions of the intensity of fluorescence modulation in virtue of multiple quenching factors conform to the rule of addition. The response feature parameters of chemical sensor based on fluorescence quenching for analyses could be calculated by combining the multivariate linear model with a tri cup method. A conception based on regarding the chemical sensor as the second source of light was advanced to get more useful information on analytes. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the chemical sensor to response a series of the therapeutic drugs. A train of thought for constructing the quantitative analysis model for the chemical membrane sensors based on the fluorescence quenching was afforded in the light of the experimental results.国家自然科学基金!(批准号 :2 9775 0 2 2

An attempt of peptidome/proteome analysis from in vitro to in vivo

An attempt of peptidome/proteome analysis from in vitro to in viv