Study on Diatom Composition of Mangrove Region at Jiulong River Estuary,Fujian Province

[目的]初步分析了福建九龙江浮宫红树林区的硅藻组成。[方法]此次研究分析鉴定了浮宫红树林区6个表层样和1个柱状样的硅藻属种组成。[结果]1浮宫红树林沉积硅藻植物群属种多样性强,共鉴定出硅藻34属103种(含不定种),大部分为中心纲的圆筛属;2红树林区底泥中硅藻数量丰富。[结论]沉积硅藻种群以浮游的海水种和咸水半咸水种为主,说明浮宫红树林处于以海水作用为主的沉积环境中。[Objective] Diatom composition of mangrove region at Jiulong river estuary,Fujian Province was preliminarily studied.[Method]We analysed and identified 6 surface sediment samples and 1 core sample,which are located in the Fugong mangrove.[Result]The diatom assemblages are diverse and a total of one hundred and three diatom species and varieties belonging to 34 genera were identified which are dominated by Coscinodiscus.[Conclusion]The diatom assemblages are dominated by marine planktonic species and brackish planktonic species indicating that the seawater plays more important role in the sedimentary environment of Fugong mangrove.国家自然科学基金项目“红树林湿地底栖动物多样性和环境效应机制研究”(41176089

生物传感器对抗戊型肝炎病毒单克隆抗体部分特性的研究

目的　应用生物传感器对新制备的抗戊型肝炎病毒单克隆抗体 (mAb)的亲和力、Ig亚类 (型 )及抗原结合的动力学进行研究。方法　用HEVORF2区基因工程重组蛋白NE2 ,免疫BALB/c小鼠 ,经杂交瘤技术制备mAb ,采用ELISA、Westernblot和生物传感器鉴定其有关特性。结果获得 12株可稳定分泌抗NE2mAb的杂交瘤细胞系。用ELISA及生物传感器等鉴定各株mAb ,分别为IgM和IgG1、IgG2a,轻链均为κ型。其中在用ELISA法对mAb3F5亚类鉴定过程中 ,发现其与HRP GAMIgG2a和HRP GAMIgM均有反应 ,生物传感器鉴定其为IgM。Westernblot验证各株mAb的特异性 ,同时各株mAb对于不同聚合形式的NE2蛋白的反应性有一定的差别。应用生物传感器测定了 4株mAb的KD 值 ,mAb 8C11为 4 .36× 10 7,mAb8H3为 1.5 3× 10 5,mAb 13D8为 1.2 1× 10 6,mAb 16D7为8.0 3× 10 7。从生物传感器测得的各株mAb与NE2的结合、解离过程中发现 ,mAb 8H3与NE2可快速结合、快速解离 ,其它 3株mAb结合稳定。结论　经多种方法鉴定 ,所获得的 12株杂交瘤细胞分泌的抗体 ,为抗HEVORF2区段的特异性抗

大肠杆菌重组颗粒性戊型肝炎疫苗对恒河猴的免疫保护

大肠杆菌表达的戊型肝炎病毒 (HEV)衣壳蛋白ORF2片段HEV 2 39重组蛋白颗粒 ,经铝佐剂吸附后 ,分别以5 μg、1 0 μg和 2 0 μg剂量免疫恒河猴 ,2 8天时以相同剂量加强免疫 1次 ,3周后分别以不同病毒滴度的基因Ⅰ型或基因Ⅳ型HEV静脉攻击。结果 ,加强后 3周 ,3个免疫剂量组猴的抗体几何平均滴度分别为 1∶2 71 75、1∶344 0 9、1∶4 16 0 7,以世界卫生组织参比血清定量 ,则分别为 1 0 98IU/ml,1 35 7IU/ml、1 72 4IU/ml。每个剂量免疫组及对照组各有 3只猴接受 1 0 7病毒滴度基因Ⅰ型HEV感染 ,对照组 3只猴均被成功感染 ,2只出现肝炎 ;2 0 μg免疫组 3只猴均未被感染 ;1 0 μg和 5 μg免疫组各有 2只猴未被感染 ,另 1只猴出现短暂感染 ,免疫猴均未出现肝炎。 3个剂量免疫组及对照组另外 3只猴 ,接受 1 0 4病毒滴度基因Ⅰ型HEV感染 ,对照组 3只猴均被感染 ,1只出现肝炎 ;而免疫猴均未被感染 ,也未出现肝炎。 3只 1 0 μg免疫猴和 3只对照猴分别接受 1 0 7病毒滴度基因Ⅳ型HEV感染 ,对照组均被感染并出现肝炎 ,而免疫组均未出现肝炎 ,有 2只未被感染 ,另 1只被短暂感染。另有 3只 1 0 μg免疫猴和 3只对照猴分别接受 1 0 4病毒滴度基因Ⅳ型HEV感染 ,对照组均被感染 ,而免疫组均未被感染。这些结果表明 :HE

埼玉県行田市埼玉愛宕山古墳の測量・GPR調査

早稲田大学東アジア都城・シルクロード考古学研究所 デジタル調査概報 第4

Study on Filmy Flow Model and Minimum Wetting Rate for Packed Column

根据液体在填料表面膜状流动的特点 ,建立了填料塔的膜流模型 ,并将该模型用于填料塔最小润湿速率的研究。结果表明 ,填料塔的最小润湿速率与填料大小无关 ,而与堆积方式有关。文中提供的最小润湿速率算式可以作为确定填料塔喷淋密度的依据Depending on characteristics of liquid flowing over the surface of packing,a filmy flow model is developed,by which the minimum wetting rate for packed column is studied.Results show the minimum wetting rate changes with the stacking manner but does not with the packing size,and our developed model is available for determining the spray density of packed column

共球藻纲丝状藻中国新记录属种———洪泛拟寇丽藻

从甘肃省张掖市黑河流域分离了一株不分枝的丝状绿藻,对其进行纯培养并保藏于中国科学院淡水藻种库,编号FACHB-2451。形态观察表明,该藻株具有相对较长的营养细胞(细胞长为宽的3~8倍)、双叶形的片状叶绿体、末端细胞顶端钝圆或略尖细等特征。结合形态比较和基于18SrDNA基因序列的系统发育分析,确定该藻株为中国淡水共球藻纲新记录属种———洪泛拟寇丽藻(Koliellopsis inundata Lokhorst)。分子系统发育研究结果表明,所分离的藻株与分离自比利时与荷兰边境农地的洪泛拟寇丽藻模式藻株序列相似度极高,且拟寇丽藻属与寇丽藻属、针丝藻属和拟针丝藻属等不分枝丝状拟寇丽藻科成员亲缘关系密切,可以较好地聚成一个亚支

Distribution characteristics of diatom in surface sediment and its relation with environment factors in Sansha Bay of Fujian

通过对三沙湾24个站位表层沉积物样品进行硅藻分析,共鉴定硅藻31属84种(包括变种和变型),其中夏季和冬季分别鉴定出31属71种和31属67种,主要属种以中心纲硅藻为主,如爱氏辐环藻(ACTInOCyCluS EHrEnbErgII)、波状辐裥藻(ACTInOPTyCHuS undulATuS)、横滨盒形藻(bIddulPHIA grundlErI)、中心圆筛藻(COSCInOdISCuS CEnTrAlIS)等.在生态习性上,底栖海水种占绝对优势.硅藻丰度从湾顶向湾外递减,夏季丰度高于冬季,平均丰度分别为9 003个/g和6477个/g.沉积物粒度分析显示,三沙湾表层沉积物以粉砂质粘土为主,研究表明三沙湾硅藻丰度与平均粒径存在一定相关性,夏季硅藻丰度变化范围为2 104~28 209个/g,粒径范围为5.26~7.36Φ,硅藻丰度与平均粒径变化范围都较大;而冬季硅藻丰度变化在1 929~14 372个/g之间,粒径范围为5.51~6.98Φ,相对夏季其变化较小,论证了水动力是影响硅藻沉积分布的一个重要因素.对影响三沙湾表层沉积硅藻分布的主要环境因子进行探讨,三沙湾表层沉积硅藻主要受潮流、深度和盐度等影响较大.Diatoms were analyzed from 24 surface sediment samples in Sansha Bay.In total,84 species belonging to 31 genera of diatoms were identified.Among them 71 species belonging to 31 genera collected in summer and 67 species belonging to 31 genera collected in winter were mainly Centricaes.The dominant groups are ctinocyclusehrenbergii,Actinoptychus undulates,Biddulphia grundleri and Coscinodiscus centralis.In view of ecological characters,the diatoms are dominated by marine epipelic species.What's more,the abundance of diatom in surface sediments decreased sharply from northern area to the entrance of the bay and the abundance in summer is higher than winter.The average abundance are 9 003 cells / g and 6 477 cells / g,respectively.The sediment grain size analysis shows that the surface sediment grain size are basically Silty clay and the study shows that diatom abundance have a certain correlation with average grain size.Diatom abundance range from 2 104 to 28 209 cells / g,Grain size varies greatly and ranges from 5.26 to 7.36Φ in summer.Diatom abundance ranges from 1 929 to 14 372 cells / g in winter.Grain size changes from 5.51 to 6.98Φ and it varies less than that in summer indicating that the hydrodynamic force is an important factor influencing the diatom distribution.The main environmental factors are also discussed in the surface sediment diatom distribution and the surface sediment diatom of Sansha Bay is controlled by tidal current,depth and salinity.国家海洋局公益性行业科研经费资助项目(201205009

Temporal and spatial variation characteristics of inorganic nitrogen and active phosphorus and relations with environmental factors in Sansha Bay of Fujian in summer and winter

根据2012年8月和2013年1月对三沙湾海域开展的海域水质现场连续调查数据,阐述了无机氮和活性磷酸盐含量时空变化特征及其与环境因子的相关性.结果表明,三沙湾海域夏冬季无机氮含量范围分别为0.059~1.669、0.569~0.940 Mg/dM3,均值为0.582、0.676 Mg/dM3,活性磷酸盐含量范围分别为0.036~0.071、0.050~0.070 Mg/dM3,均值为0.056、0.060 Mg/dM3.无机氮和活性磷酸盐含量的高值区出现在陆源径流入海区及大量网箱养殖区,总体呈湾顶向湾口递减趋势,氮磷含量表层高于底层,冬季高于夏季,大部分海域都超过第四类海水水质标准.根据Cn/CP比值评价结果显示,三沙湾大部分海域浮游植物的生长为氮限制.相关性分析表明,三沙湾海域无机氮与硝酸盐含量相关性极显著(r=0.989),表明硝酸盐是无机氮的主要存在形态;无机氮还与盐度(r=-0.923)和温度(r=0.939)有极显著的相关性,论证了沿岸径流对三沙湾营养盐的输入有重要影响.活性磷酸盐与铵盐、亚硝酸盐含量存在显著的正相关.本次调研结果为三沙湾海域富营养化研究提供基础数据.Continuous field data were taken at 6 stations in August,2012 and January,2013 in Sansha Bay.Temporal and spatial variation characteristics of inorganic nitrogen and active phosphorus,as well as their correlations were presented.The results confirm that the content of inorganic nitrogen varies from 0.059 to 1.669,from 0.569 to 0.940 mg/dm3,and the average values are 0.582 and 0.676 mg/dm3 in summer and winter,respectively.The content of PO4-P are in ranges from 0.036 to 0.071,from 0.050 to 0.070 mg/dm3,the mean value are 0.056 and0.060 mg/dm3,respectively.The high values of DIN and PO4-P appeared in areas receiving the terrestrial runoff and areas of the cage culture,the value trends down from northern area to the entrance of the bay.The value is higher in surface water than the bottom and higher in winter than in summer,with most of areas exceed the fourth grade of seawater quality standard of China.The result of CN/CPratio shows that most phytoplankton growths are nitrogen limited.The correlation analysis shows that DIN is significantly correlated with NO3-N(r = 0.989),salinity(r =-0.923) and temperature(r = 0.939).PO4-P is also positive correlation with NH4-N and NO2-N.This study supplies basic information for the eutrophication research in Sansha Bay.国家海洋局公益性行业科研经费资助项目(201205009

A Peripheral Edge Detection Method for Multiple Indexable Inserts

针对可转位刀片周边刃边缘检测的问题,提出了一种适用于多个可转位刀片的基于局部模糊检测和双树复小波变换的边缘检测算法。该算法主要分为两步,第一步是先按事先规定的要求拍摄可转位刀片的图片,然后对拍好的图片进行局部模糊检测处理,其中的检测算法是以图片中像素的相关性系数和基于Laplacian算子的新型判别算子为评价指标判断其是否位于模糊区域（若位于模糊区域,则用白色显示它,反之,则用黑色显示）。模糊检测算法在剔除误检像素后最后会生成一副二值图,可转位刀片的周边刃边缘即为二值图中黑色区域和白色区域的交界线。第二步就是用双树复小波变换对二值图进行边缘检测,检测后的结果就是我们需要的结果。实验结果证明,该算法能准确快速地检测出多个可转位刀片的周边刃边缘。As for the problem of peripheral edge detection for indexable inserts , this paper propose an algorithm of edge detection based on local-blur detection and dual-tree complex wavelet transform for multiple indexable in- serts. The algorithm have two steps： the first step is to take pictures of indexable inserts according to the require- ments specified in advance firstly,and then make local-blur detection in the picture which was been taken,here the algorithm of edge detection judge whether the pixel is in the blur area with the correlation coefficients of the pixel in the picture and the new operator based on laplaeian operator as evaluation indexs （if it is in the blur area, show it in white; otherwise, show it in black ）.After eliminating the mistake pixels, the last step of the algorithm of edge detection will create a binary image,the peripheral edge of indexable inserts are the demarcation lines be- tween white areas and black areas in the binary image.The second step is to detect the edges in binary image by using dual-tree complex wavelet transform,the result ater transform is that we need. Experimental results show that this method can detect the peripheral edge of multiple indexable inserts quickly and accurately.闽发改投资[2016]482

巢湖蓝藻水华的季节变化

<正>巢湖是中国第5大淡水湖,位于安徽省中部。100多年前已有记载:到了夏天,巢湖水面上漂浮有"湖靛",即现在称作的蓝藻水华。历来,湖边农民捞这种湖靛作为肥料。这表明,巢湖蓝藻水华出现在环境污染严重化之前。上世纪90年代后,随着环境污染的日趋严