Research on the Anti-perimenopausal Depression Mechanism of Chaiyu Dixian Prescription

目的： 1.观察卵巢去势手术与慢性轻度不可预见性应激对大鼠的影响，探讨围绝经期抑郁症的发作与生理因素、环境应激的关系。 2.观察柴郁地仙方对围绝经期抑郁症模型大鼠的药理效应；从单胺类神经递质、HPO轴及血清微量元素方面探讨柴郁地仙方抗围绝经期抑郁症作用机制。 方法： 通过对健康成年雌性大鼠行双侧卵巢去势手术建立内源性雌激素撤退围绝经期模型，在此基础上通过孤养及21天慢性轻度不可预见性应激复制大鼠抑郁模型。实验动物分为假手术组、去势组、模型组、氟西汀组、雌二醇组、柴郁地仙方组，应激过程给药23天。通过大鼠体质量、敞箱实验得分及糖水消耗量评价大鼠行为学变化；采用ELISA法检测大鼠血清E2...Objective: 1. Observe the effects of ovariectomy surgery and chronic mild chronic stresses (CUMS) in rats, to explore the relationship between environmental stresses, physiological factors and the emergence of permenopausal depressive disorder. 2. Observe the pharmacological effect of using Chaiyu Dixian Prescription to treat rats with permenopausal depressive disorder; to investigate the mechan...学位：医学硕士院系专业：医学院_中医妇科学学号：2452014115358

Molecular cloning, expression of heat shock protein 60 (HSP60) gene in Marsupenaeus japonicus and the association of its polymorphisms with thermal tolerance

日本囊对虾(Marsupenaeusjaponicus)是我国重要经济养殖虾类，但其在南方地区存在着高温度夏死亡率较高的养殖难题。因此，急需了解日本囊对虾的耐热性状及耐温的分子机制，为此，我们开展了日本囊对虾MjHSP60基因的克隆、表达分析及其多态性与耐热性状相关性的研究，获得了如下主要结果： 1.MjHSP60基因克隆 在日本囊对虾MjHSP60cDNA序列基础上克隆到了该基因DNA全序列。日本囊对虾MjHSP60基因全长4055bp，结构与拟穴青蟹一致，均由8个外显子及7个内含子构成，且对应的外显子长度极其相近，序列相似度达94%，而内含子长度存在较大差异。 2.MjHSP60mR...Marsupenaeus japonicus is an important commercial species in China, however, high temperature has been a negative factor for M. japonicus farming in summer. Therefore, it’s important to understand the thermal resistance and mechanism of heat resistance of M. japonicus. In the present study, a HSP60 gene from M. japonicus was cloned, the expression of MjHSP60 mRNA during early developmental stages ...学位：理学硕士院系专业：海洋与地球学院_海洋生物学学号：2232012115132

Environmental Geochemistry Characteristics of Phthalate Esters in the Jiulong River and its Estuary, Fujian, China

邻苯二甲酸酯（Phthalateesters，PAEs）是一种普遍存在的全球性污染物，具有内分泌干扰效应和“致畸、致癌、致突变”作用。PAEs难溶于水，进入水环境后易吸附于颗粒物而进入沉积相，或被水生生物吸收而蓄积于生物体内。河流及河口作为连接陆地与海洋之间的过渡带，是陆源污染物质输送至海洋的最重要通道，研究PAEs在陆地河流及入海河口的污染状况、迁移过程、输送通量以及污染历史，是环境地球化学的重要内容，可为河流、河口及海洋生态环境PAEs的污染控制提供重要的理论依据。 论文选择九龙江及其河口为研究区域，以PAEs为研究对象，系统研究2014年4月-2015年1月期间，该区域PAEs在丰水期...Phthalate esters (PAEs) are one group of pollutants most widely used worldwide, which have endocrine disruptors, teratogenic, carcinogenic and mutagenic effects. Due to their hydrophobic properties, PAEs in the aquatic environment are apt to absorb to particles and accumulate into sediment, and also easily absorbed by aquatic organisms and accumulated into their body. River and estuary, as the tra...学位：理学博士院系专业：环境与生态学院_环境科学学号：3312012015355

The analysis of securities margin trading’s impact on china stock market volatility

2010年3月31日，融资融券交易的正式推出，结束了我国股票市场长期以来的“单边”格局。对于融资融券交易机制的引入能否提高我国股票市场的运行质量，学术界及业内人士的看法不一。鉴于此，本文通过选取上海股票市场的相关交易数据进行分析，试图探究推出融资融券交易对于国内股票市场的影响。 本论文首先整理并分析国内外融资证券交易的相关资料，确定研究思路和方向；其次，对融资融券含义、发展过程、交易方式、对市场的影响作用，股票市场波动性、流动性的影响因素及融资融券交易对于股票市场流动性和波动性影响进行具体解析，为实证研究奠定基础；然后，以上海股票市场作为研究对象，利用VAR模型、格兰杰（Granger）因果...On March 31,2010,the official launch of securities margin trading, ended the China Stock Market "long-term unilateral" pattern. Whether the introduction of securities margin trading can improve the operation of China Stock Market,academics and insiders have different opinions. In view of this, the paper attempts to explore the influence of securities margin trading by analyzing the deal date of Sh...学位：金融硕士院系专业：经济学院_金融硕士学号：1562013115213

A Study of Advertising Research

本研究采用内容分析的方法，以美国广告学术期刊《JournalofAdvertisingResearch》为研究对象，通过对该杂志文章标题、作者人数、作者国别、发表年份的分析，全面系统地探讨30多年来国际上广告研究的发展状况及趋势，期望为今后的广告理论建构和广告实践活动和提供新的指导意义，并为中国广告理论研究的发展进步提供借鉴经验。研究结果显示：①30多年来，研究者们对于广告研究的热情一直在不断提高之中，其研究生产力也在不断发展之中。同时，JAR杂志为研究者发表、共享研究结果提供了更大空间。②在广告研究涉及的10个领域中，各领域得到的研究关注轻重不一。广告媒体和消费者行为是最受关注的两个研究领域...The study used content analysis to reach the purpose. It chose ‘Journal of Advertising Research ’, which is one of the three leading Advertising Journals in American, as the research object. The purpose is to entirely investigate the development and trends of advertising research of the world during 30 years, to provide new guidance for the establishment of advertising theory and advertising activ...学位：文学硕士院系专业：人文学院新闻传播系_新闻学学号：20033203

The effect of low glucose and high lactic acid on the survival of Hela

目的:探讨低糖高乳酸环境对宫颈癌细胞生存的影响,以及对EGFR-mTOR通路的调节作用。方法:将Hela细胞培养于常糖(葡萄糖10mmol/L); 、低糖(葡萄糖3mmol/L)、高乳酸(葡萄糖10mmol/L,乳酸2.5mmol/L)、低糖高乳酸(葡萄糖3mmol/L,乳酸2.5mmol/; L)4种环境下,CCK-8法测定Hela细胞的生长抑制率,流式细胞术测定细胞周期。荧光实时定量PCR法检测EGFR和mTOR; mRNA水平表达。结果:与常糖组相比,高乳酸组的细胞抑制率显著升高(P<0.01),48h细胞G_1/G_0期比例显著升高(P<0.01),细胞; 凋亡率、EGFR和mTOR; mRNA表达水平均无变化。与常糖组相比,低糖组的细胞抑制率显著升高(P<0.01),48h细胞凋亡率显著降低(P<0.01),细胞各周期比例变化; 与常糖组无差异,EGFR表达水平降低(P<0.05)。与常糖组相比,低糖高乳酸组的细胞抑制率显著升高(P<0.01),但低于低糖组(P<0.01; )和高乳酸组(P<0.05),48h G_1/G_0期比例显著升高(P<0.01),细胞诱导凋亡率显著升高(P<0.01),EGFR和mTOR; mRNA表达水平均显著升高(P<0.01)。结论:Hela细胞在低糖高乳酸环境中的存活状况好于单纯低糖和单纯高乳酸环境,且伴随着EGFR和mTO; R基因表达水平上升。Objective:To explore the effect of the low glucose and high lactic acid; environment of survival of cervical carcinoma cells,and the regulation; of EGFR-mTOR signal way.Methods:Hela cells were cultured in four; conditions:normal glucose (glucose 10mmol/L),low glucose (glucose; 3mmol/L),high lactic acid (lactic acid 2.5mmol/L) and low glucose add; high lactic acid (glucose 3mmol/L,lactic acid 2.5mmol/L).Growth; inhibition rate of Hela cell was determined by CCK-8.Flow cytometry; (FCM)were performed to evaluate the cell cycle.The expression levels of; EGFR and mTOR mRNA were detected using real-time quantitative polymerase; chain reaction (real-time PCR).Results:Compared with those in regular; sugar environment,the cell growth inhibition rates were significantly; increased in high lactic acid environment(P<0.01).At 48h,the rate of; G_1/G_0 was significantly increased (P<0.01),while the apoptosis rate of; the cells and the expressions of EGFR and mTOR mRNA had no; change.Compared with those in regular sugar environment,the cell growth; inhibition rates were significantly increased in high lactic acid; environment,(P<0.01),while lower than those in low glucose; environment(P<0.01) and in high acid environment(P<0.05).The rates of; G_1,G_2 and S phase had no change.The expressions of EGFR mRNA was; reduced (P<0.05).The cell growth inhibition rates were significantly; increased in high lactic acid add low glucose environment (P<0.01).At; 48h,the apoptosis rate and the rate of G_1/G_0 were significantly; increased (P<0.01),while the EGFR and mTOR expression levels were also; increased (P<0.05).Conclusion:Hela in the low glucose add high lactic; acid survives better than those in low glucose and in high lactic acid; environment,while the expression of EGFR and mTOR is increased.泉州市科技计划立项项目; 国家面上项

The Review and Prospect About the Design of WuShu Content in PE

采用文献资料法和调查研究法,对福建省14所中小学和我国92所普通高校武术教学内容设置进行调查。在回顾建国以来我国学校武术教学内容设置历程的基础上,分析探讨当前大中小学校武术教学内容设置与教学存在的问题,并对新时期学校武术教学建设的的、课程目标、教材建设、教学模式、教学评介等方面提出展望。This article presents the research of the teaching content and method about WuShu in part colleges and the part primary schools and middle schools in FuJian.On the historical basis of the design of the teaching content about colleges,middle schools and primary schools in modern times,author analyses the problems existing in ti and puts forward some ideas about guiding ideology,curriculum objective,construction of teaching material,teaching model,teaching appraise and so on