Preparation andProperties of Fluorescent Gold Nanoclusters Functionalized Silk Biomaterials

家蚕蚕丝作为天然的蛋白质纤维，具有独特的力学性能、良好的生物相容性和可控的生物降解性，已被作为生物医学理想材料。然而，蚕丝材料作为生物医学材料植入体内后，进行体内的检测和追踪仍是目前一大难点。本研究通过共混荧光金纳米簇和丝素蛋白，制备可用于体内荧光成像的蚕丝材料（3维支架、薄膜、凝胶），为生物材料的体内成像提供了思路。本研究主要内容如下： 冷冻干燥法制备三维多孔荧光丝素蛋白/金簇复合支架。复合支架具有红色荧光，研究发现丝素蛋白/金簇混合溶液的pH会影响金簇的荧光强度，发射峰位和荧光效率，支架荧光量子产率可达18%。金簇的加入增加丝素蛋白β-折叠和结晶度。丝素蛋白/金簇复合支架的孔洞之间相互贯...Bombyx mori silk fibers, as a natural protein, have been adsorbing great interests in the fields of biomedicine, due to robust mechanical properties, excellent biocompatibility and controllable biodegradation. However, the monitoring of the evolution of the overall structure and organization of the materials in vivo or in vitro remains as a neck bottle for the further refinement of silk bio-materi...学位：理学硕士院系专业：材料学院_生物医学工程学号：3142013115012

Functionalization of Silkworm Silk Materials in Optics

家蚕蚕丝是一种天然的蛋白质纤维,蚕丝及其衍生物(凝胶、蚕丝薄膜和海绵等)具有独特的力学性能、良好的生物相容性和生物降解性,已经被应用在纺织、生物医学等领域。最近,利用蚕丝独特的性质制备光学功能化蚕丝吸引了研究者的兴趣。基于对蚕丝的丝胶和丝素结构组成的总结,介绍了目前蚕丝结构的相关研究进展,综述了近年来对光学功能化蚕丝的研究,具体包括光学功能化蚕丝的重要种类、制备和应用。重点介绍了荧光性功能蚕丝基因工程、喂食法和复合等制备方法,该种材料用于生物支架成像、药物缓释载体和光学器件等领域。总结了利用生物仿生技术制备具有光子晶体结构色的蚕丝材料,这为化妆品,变色服饰和生物光控器件开辟了新方向;此外,蚕丝材料还可以应用于非线性光学领域,如光限幅和激光防护。As a kind of nature protein,silkworm silk and its derivatives( gels,silk fibroin foams and films) possess the unique mechanical properties,biocompatibility and biodegradability,which have been applied to textile and biomedical industry.Recently,there is a growing interest in introducing optical functionalities into silk.Based on the structure of sericin and fibroin,functionalization of silk was summarized in optics,specifically including the important species,preparation and application of fluorescent silk.This paper highlighted that the fluorescent silk can be produced by genetic engineering,feeding,and compositing etc.,which can be applied in scaffold imaging,drug delivery,and optical devices.In addition,this paper reviewed the fabrication of silk fabrics with photonic crystals structure colors using biomimetic techniques,which opens up a new direction of cometic,color dress,various devices and biological optical devices.At last,silk materials can also be applied in nonlinear optics,such as optical limiting and laser protection.厦门大学中组部千人计划项目配套经

纳米金簇复合丝素薄膜的性能及其在生物成像中的应用

具有荧光性能的丝素薄膜可用于生物体内外实时的、无损伤的生物成像,为直观监测体内生物材料及其化学环境的变化提供了新思路。采用浇铸法制备纳米金簇复合丝素薄膜,研究了纳米金簇、甘油和制备过程中pH值对纳米金簇复合丝素薄膜结构和性能的影响。结果表明:纳米金簇和甘油的加入都会提高纳米金簇复合丝素薄膜β-折叠和结晶度,从而提高其断裂强度;在碱性条件下制备的纳米金簇复合丝素薄膜在680nm处可发射较强荧光;细胞能在制备的薄膜上正常黏附和生长,实现薄膜和细胞同时荧光成像。国家自然科学基金资助项目(51773171,U1405226)福建省科技厅自然科学基金资助项目(2017J06019)高等学校学科创新引智计划资助项目(B16029)厦门大学校长基金资助项目(20720160088,20720150218