Preparation of Monoclonal Antibody against Stevioside and Development of ELISA Method for Stevioside

甜菊苷(stevioside，ST)为甜叶菊中所含的主要甜味成分，是理想的高甜度、低热量，对糖尿病和高血糖脂有很好疗效的一种新型高安全性甜味剂，也可作为一些药品的品位改良剂和矫味剂，从而广泛应用于食品和药物。目前，甜菊叶中甜菊苷的提取工艺已经日趋成熟，然而甜菊苷的准确含量的检测方法主要是使用HPLC，还存在不足之处，因此，建立简单，快速，准确的甜菊苷定量方法对于甜菊苷的生产和应用都具有极其重要的意义。 本研究利用高碘酸纳法将甜菊苷（ST）分别与人血清白蛋白（HSA）和牛血清白蛋白（BSA）进行偶联，成功制备了免疫抗原和包被抗原，经MALDI-TOFMS鉴定得HSA，BSA上分别挂13，8个S...Stevioside, as the major sweet ingredient of Stevia rebaudiana, is a new kind of natural high sweetness, low-calorie and high safety sweetener, which has physiological functions including anti-hyperglycemia and anti-diabetes. It’s also can be used as improving the tastes of medicine. Its stable chemical and physical properties and non-fermentability lead to long shelf life and widely using in food...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162007115201

The cause of truth of science being asleep and references

科学史上,有不少“真理沉睡“的事例。分析发现:既有内在原因,也有外在原因;内在原因是理论自身表述不够完善,缺乏应有的实践指称,相关学科发展滞后,仪器技术水平落后等;外在原因是意识形态的遏制、权威的压制、范式理论的束缚和时代认识水平的限制等。从中可以得到如下启示:作为年轻的科学工作者要敢于冲破范式理论的束缚,学术上应该追求完善,面对权威和习俗的阻力需要策略和冷静;作为知名科学前辈要有与时俱进的思想品格和精心育人的意识。In the history of science,there are many sleeping examples of truth.Through analyzing found that:there are both internal causes and external factors;the internal causes are that the theory itself are inadequate representation,lack of proper allegated practices,the relevant disciplinary subject development and the technological level of equipment lag behind,etc.;the external reasons are that the impact of ideologies,the repression of authorities,the restrictions of traditional ideas and contemporary level of awareness.From these analyses we can obtain the following references: as a young science workers must dare to break through the traditional concept,pursue academic excellence,facing resistances from authorities and customs need the policies and calming;as a well-known scientific predecessors need change by the times and educate youths carefully

Evolution of Scientific Models and Its Characteristics of Epistemology

模型是人们科学理解和解释研究对象的重要桥梁。模型作为对研究对象“数学筹划“的结果,必然具有简约近似的特征。模型的不同形态和精细程度表征了科学研究的不同发展阶段。随着科技的发展,科学模型从实体到观念化、从理论到数学化、从定性到定量、从计算机模拟到仿真,逐步从形象变得抽象,最后却又从高度抽象回归到形象。另外,理解科学模型具有的主体性、诠释学特征,这对于理解当今科学哲学发展的新动向具有启发性。In scientific research,model is an important bridge to understand and explain the objects of study.Model,as the research object,is the result of "mathematical projection" and it is bound to be simple and approximate.The different forms and sophistication degree of Models show different stages of development in scientific research.With the development of science and technology,science models have evolved from being physical to conceptualized,theoretical to mathematical,qualitative to quantitative,and computer simulation to reality simulation,which means a gradual development from concrete to abstract,but finally returned to concrete from the high level of abstraction.This process reflects the essential requirements of visualization nature of human thinking

一种自供电多传感器无线环境质量监测系统的设计

为了实时精确监测既定区域的环境质量状况,解决传统无线传感网因电池不耐用又不能及时更新而导致网络中断的问题,研究设计了一种自供电多传感器的无线环境质量监测系统。所研究设计的无线传感网节点利用太阳能光伏电池板发电完成自供电,并对最大功率点跟踪控制算法进行了优化改进,解决了因电池不能及时充电而导致的传感器节点工作异常的问题,实现了环境质量状况无人值守实时监测。利用数据融合算法,将多节点传感器采集到的数据进行数据融合,通过实验测试表明,所设计系统的测试数据和当地当天中国环境监测总站发布的环境质量状况数据基本吻合。复杂系统优化与大数据处理广西高校重点实验室科研课题资助(No.2017CSOBDP0103);;广西高校科学与技术研究项目(201012MS185);;贵州省科学与技术基金(LKS[2012]34

In-situ SERS of formic acid electro-catalytic oxidation on Au-core@Pt-shell/Pt electrode

Au-core@Pt-shell nanoparticles were synthesized by chemical reduction method. The samples were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). In-situ surfac-enhanced Raman scattering (in-situ SERS) spectroscopy combined with cyclic voltammetry was utilized to investigate the electro-oxidation behavior of formic acid adsorbed on Au-core@Pt-shell nanoparticles coated on platinum electrode, and SERS spectra with high quality were acquired. Results showed that the intermediates COad and HCOOad, the dissociated products of HCOOH, were produced at open circuit potential. As the potential moved to positive, the first oxidation wave for CO, was observed at +0.10 V. The study demonstrated that the electrode prepared by Au-core@Pt-shell nanoparticles coated on platinum substrate exhibited good electro-catalytic properties and SERS activity for the oxidation of HCOOH, and the electro-oxidation proceeds of formic acid via "dual path" mechanism