Ocean surface wind and wave monitoring at Typhoon Fung-Wong by HFSWR OSMAR071

介绍了OSMAr071高频地波雷达系统“凤凰“台风期间海面风、浪遥测结果。文中给出高频地波雷达OS-MAr071风、浪反演算法:利用SbM模型结合多波束采样法反演风向;利用改进的bArrICk模型进行有效波高的反演;利用SMb关系式进行风速反演。将雷达定点观测结果与浮标数据进行对比,表现出很好的一致性,其中有效波高相关系数为0.72,均方根误差为0.48M,风向相关系数为0.97,均方根误差为27.7°,风速的相关系数为0.6,均方根误差为3.5M/S。文中还对影响探测精度的因素进行了分析。This paper provides wind and wave observation results detected by high frequency surface wave radar OSMAR071 during Typhoon Fung-Wong.Methods for extracting ocean surface parameters from HFSWR sea echo are introduced.The Stewart Barnum and Maresca SBM method combined with multi-beam sampling method is used to invert wind direction.A modified Barrick's model is proposed to obtain significant wave height and the SMB formulation is used to extract wind speed information.Compared with buoy data of wind and wave,radar inversion results show good agreement with in-situ observations.The root-mean-square error RMSE of significant wave height,wind speed and wind direction between radar-derived and those from the buoy are 0.48 m,3.5 m/s,27.7°,and the coefficients of determination(R2) are 0.72,0.60 and 0.97,respectively.Factors affecting detecting accuracy are also discussed in this paper.国家高技术研究发展计划(863计划)(编号:2009AA09A301);国家自然科学基金(编号:60571065)---

Preparation of monoclonal antibodies against 3D protein of EV71 based on HBc particles as expression vector

目的:预测肠道病毒71型(EV71)非结构蛋白3D的表位,以HBc蛋白为载体展示多肽,制备并鉴定抗EV71-3D的特异性单克隆抗体(mAb); 。方法:应用生物信息学方法分析预测出EV71; 3D蛋白亲水性和免疫原性指数较高的多肽片段,并运用HBc颗粒型蛋白载体展示肽段,构建多肽融合蛋白,免疫BALB/c雌鼠,通过杂交瘤技术和亲和层析; 技术制备和纯化抗EV71-3D蛋白的特异性mAb,用间接ELISA、ELISPOT、IFA和IHC对mAb的性质进行初步鉴定。结果:构建表达分别; 嵌合3D蛋白34~ 43位氨基酸残基、61~ 76位氨基酸残基、151~; 164位氨基酸残基的HBc重组蛋白,免疫并经过多轮克隆化筛选,获得抗EV71-3D单克隆抗体3E1,其亚类为IgG2a;免疫荧光试验、ELISP; OT法和免疫组织化学染色结果显示其可与EV71特异性结合。结论:成功制备可特异性识别EV71的单克隆抗体3E1,为病毒的检测及进一步研究3D蛋白; 的功能奠定了基础,同时还验证了生物信息学技术与HBc颗粒型载体展示多肽技术相结合可快速高效地制备单克隆抗体。Objective: To prepare and preliminarily identify the monoclonal; antibodies(mAbs) specifically against 3D protein of Enterovirus; 71(EV71),using bioinformatics to predict the epitopes of 3D,with HBc; protein as a carrier.Methods: Artificial screening of 3D protein epitope; sequences by bioinformatic method,inserted into the major immunodominant; region(MIR) area of Hepatitis B virus core protein(HBc),to construct the; recombinant protein.BALB/c mice were immunized with the recombinant; virus like particles(VLPs),to prepare the mAbs against 3D protein of; EV71.Affinity chromatography technology was used to purify the mAb.The; indirect ELISA,ELISPOT,immunofluorescence and immunohistochemistry; staining methods were used to identify the characteristic of the; mAb.Results: We displayed 3D(aa34-43),3D(aa61-76) and 3D(aa151-164); epitopes by constructing fusion protein using HBc VLPs as a vector,after; hybridization,one positive hybridoma cell line(3E1) was selected by; ELISA.The isotype of 3E1 was IgG2a.The results of immunofluorescence and; immunohistochemistry staining assay showed that the mAb 3E1 could; specifically recognize EV71.Conclusion: The prepared mAb 3E1 can; specifically recognizes the EV71,which laid the foundation for the; detection of virus and further study on 3D protein,and verified the; bioinformatics technology combined with HBc carrier displaying peptides; could prepare mAb quickly and efficiently.国家自然科学基金项

The Difference of Luminous Performance Between Traditional Phosphor Packaging LED and Remote Phosphor LED

研究了传统白光lEd与蓝光激发的球冠形远程荧光粉白光lEd在不同电流、不同热沉温度下的发光性能,并对其机理差异展开了探讨。实验结果表明:随热沉温度和驱动电流的上升,传统白光lEd的量子效率和电光转换效率急剧下降,并导致其y/b比(yEllOW/bluE rATIO)下降,相关色温上升。而在远程荧光粉白光lEd中,其量子效率、光转换效率和相关色温在相同实验条件下变化幅度都较小。由光强空间分布和y/b比空间分布可知,远程荧光粉白光lEd的光强分布呈类似蝠翼分布,且y/b比空间均匀性远大于传统白光lEd。Under different drive current and heat sink temperature,luminous performances as well as physical mechanisms were studied for both traditional phosphor-dispensing packaging white LED and blue light-converting hemisphere remote phosphor LEDs.With the increase of the heat sink temperature and the drive currents,the quantum efficiency and light conversion efficiency in the traditional phosphor-dispensing packaging white LED drop rapidly,which is responsible for the decrease of Y/B ratio(Yellow/Blue Ratio) and the apparent increase of CCT(correlated color temperature).However,the parameters of quantum efficiency,light conversion efficiency and CCT in blue light-converting hemisphere remote-phosphor packaging LEDs have little change under the same experimental conditions.In addition,the remote phosphor LEDs exhibit a batwing spatial luminous intensity distribution and the spatial Y/B ratio distribution is much more homogeneous compared with the traditional phosphor-dispensing packaging white LED.国家自然科学基金(11104230;61102030); 福建省产学研重大科技项目(2011H6025;2013H6024); 福建省重点科技项目(2012H0039)资

Identification of antibodies with non-overlapping neutralization sites that target coxsackievirus A16

手足口病（Hand, Foot and Mouth Disease，HFMD）是一种由人肠道病毒引起的全球性传染病，主要发生于5岁以下的婴幼儿。2月5日，我校夏宁邵教授团队在《细胞》子刊《细胞•宿主与微生物》（Cell Host & Microbe）上在线发表题为“Identification of antibodies with non-overlapping neutralization sites that target coxsackievirus A16”的研究论文。该研究首次揭示了手足口病主要病原体柯萨奇病毒A组16型（CVA16）三种衣壳颗粒形式与三种不同类型的治疗性中和抗体的全面相互作用细节和非重叠的中和表位结构信息，阐明了CVA16成熟颗粒是疫苗候选主要保护性免疫原的理论基础，建立了可指导疫苗研制的免疫原特异检测方法，为CVA16疫苗及抗病毒药物研究提供关键基础。我校夏宁邵教授、李少伟教授、程通副教授和美国加州大学洛杉矶分校纳米系统研究所Z. Hong Zhou（周正洪）教授为该论文的共同通讯作者。我校博士生何茂洲、徐龙发博士后、郑清炳高级工程师、博士生朱瑞和尹志超为该论文共同第一作者。【Abstract】Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific neutralizing monoclonal antibodies (nAbs) with therapeutic potential: 18A7, 14B10, and NA9D7. We present atomic structures of these nAbs bound to all three viral particle forms—the mature virion, A-particle, and empty particle—and show that each Fab can simultaneously occupy the mature virion. Additionally, 14B10 or NA9D7 provide 100% protection against lethal CVA16 infection in a neonatal mouse model. 18A7 binds to a non-conserved epitope present in all three particles, whereas 14B10 and NA9D7 recognize broad protective epitopes but only bind the mature virion. NA9D7 targets an immunodominant site, which may overlap the receptor-binding site. These findings indicate that CVA16 vaccines should be based on mature virions and that these antibodies could be used to discriminate optimal virion-based immunogens.This work was supported by grants from the Major Program of National Natural Science Foundation of China ( 81991490 ), the National Science and Technology Major Projects for Major New Drugs Innovation and Development ( 2018ZX09711003-005-003 ), the National Science and Technology Major Project of Infectious Diseases ( 2017ZX10304402-002-003 ), the National Natural Science Foundation of China ( 31670933 and 81801646 ), the China Postdoctoral Science Foundation ( 2018M640599 and 2019T120557 ), the Principal Foundation of Xiamen University ( 20720190117 ), and the National Institutes of Health ( R37-GM33050 , GM071940 , DE025567 , and AI094386 ). 该研究获得了国家自然科学基金、新药创制国家科技重大专项、传染病防治国家科技重大专项和美国国立卫生研究院基金的资助

Private social work organization development path discussion Based on of Push-Pull Theory —take PH Organization as an example

随着当今社会迅速发展，伴随而来的社会问题越来越多，政府也在不断探索解决社会问题的办法，在此契机中，大批社会工作机构相继涌现承接政府公共服务部分职能以回应社会需求。不过，国内民办社工机构在发展过程中也面临着巨大的生存挑战，故而部分学者提出两种不同意见，一种是以依靠资源为主获得生存的发展路径，另一种则认为社工机构的诞生就是为了践行使命。 本文通过对东莞市一家大型民办社工机构的个案研究，运用推拉理论阐述PH社工机构发展的推力和拉力的影响因素，其中，政府财政资源是该机构发展的绝对拉力，与此同时，资源的控制也成为反拉力反作用于该机构；在践行使命中遇到的阻力，包含专业价值的不认同、行业生存空间的压力形成...With the rapid development of the current society, more and more problems came into being. In order to fill these requirements, a large number of social work organizations have emerged to undertake a part of functions of the government's public service. Under this situation, most of the private social work organizations are still facing a huge survival challenge. Some of the scholars put forward t...学位：社会工作硕士院系专业：公共事务学院_社会工作硕士学号：1402014115052