Si-Cu合金化渣剂精炼去除冶金级硅中金属杂质

采用Si-Cu合金精炼与CaO-SiO2-CaCl2造渣精炼相结合的方法对冶金级硅进行精炼,通过多种分析方法考察了合金造渣过程、渣剂添加剂和合金成分对金属杂质铁（Fe）、铝（Al）和钙（Ca）去除效果的影响。结果表明,提高Si-Cu合金中的Cu含量可以有效增加Cu3Si相在Si中的含量,合金造渣过程会产生多而弥散的合金相,造渣后的金属杂质Fe、Ca聚集在Si-Cu合金的Cu3Si相中。在渣剂中添加CaCl2助溶剂可有效提高金属杂质Fe、Al和Ca的去除率。此外,随着Si-Cu合金中Cu含量的增加,Cu在合金中的析出现象明显,Fe的去除率上升,Al的去除率不变,Ca的去除率下降。当Si-30%Cu合金与45%CaO-45%SiO2-10%CaCl2渣剂进行精炼后,Fe的去除率为68%,Al的去除率为94%,Ca的去除率为86%

Expression of RGS16 protein induced by wide type p53 gene in rat glioma C6 cell line

目的:探讨内外源性野生型p53是否可以诱导大鼠胶质瘤细胞C6RGS16表达。方法:在大鼠胶质瘤细胞C6中,分别转染pEGFP-C3-wtp53或以400ng/ml表阿霉素诱导内源性野生型p53表达,于处理后0h、4h、8h、16h、26h、32h和52h收集细胞爬片、固定后免疫细胞化学检测p53蛋白与RGS16蛋白表达。结果:转染pEGFP-C3-wtp53后4h、8h、16h、26h和32h时均检测到p53蛋白表达,在8h和16h时检测到RGS16蛋白表达;在400ng/ml表阿霉素处理后,仅在26h时检测到p53表达而始终未见到RGS16蛋白表达。结论:内外源性野生型p53可以诱导大鼠胶质瘤细胞C6RGS16蛋白的表达

Detection of point mutations of Axin gene and its expression in gliomas

目的　检测胶质瘤中Axin基因的点突变及其表达情况 ,初步探讨Axin与胶质瘤发生的关系。方法　采用聚合酶链反应 单链构象多态性 (PCR SSCP)技术及DNA测序方法检测Axin基因外显子 8,9及 10在 2 8例胶质瘤中的突变情况 ;同时对上述胶质瘤及正常脑组织进行免疫组化染色。结果　在 2 8例胶质瘤组织中Axin的第 10个外显子共有 6例样本 (2 1.4 % ) 3处发生了错义突变 ;3例 3处发生了同义突变 ;2 8例胶质瘤中 8例 (2 8.6 % )Axin表达阳性 ,正常脑组织中神经元表达阳性 ,神经胶质细胞表达阴性 ,检测到突变的样本中 1例表达阳性。结论　Axin基因的点突变可能参与胶质瘤的发生 【英文摘要】 Objective To detect the point mutations of Axin gene and its expression in glioma and explore the relationship between Axin gene and the occurrence of human glioma.Methods The point mutations of exon 8,9,10 of Axin gene were analyzed in 28 cases of glioma by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) analysis, silver staining and DNA sequencing. Immunohistochemistry was used to detect the Axin expression in these cases and normal brain tissues.Results Three missense poi...高等学校骨干教师计划资助项目;; 归国留学人员科研启动基金资助项目 (1 999747

Rat glioma C6 cell apoptosis induced by UV radiation via p38-MAPK

目的 :探讨 p38MAPK在紫外线损伤刺激细胞中的特异性信号转导作用 .方法 :流式细胞仪检测紫外线照射 30min后 1,2及 4h的C6细胞周期变化和是否有凋亡发生 ;应用免疫细胞化学技术观察紫外线刺激前后 p38MAPK在C6细胞中的表达强度和分布特征 .结果 :细胞周期结果显示 1,2和 4h后G1期细胞数分数各增多 0 .12 ,0 .2 1和 0 .19,而S期细胞数分数减少 0 .10 ,0 .14和 0 .15 ;各组的凋亡率分别是12 % ,4 9%和 34% ;未受刺激的细胞中 ,p38MAPK在胞质和胞核表达较弱 ;紫外线损伤作用 2h后 ,细胞核区的染色强度即明显增强 ,而胞质区域的染色强度相对降低 .结论 :C6细胞受紫外线损伤后可通过 p38MAPK通路发生凋亡. 【英文摘要】 AIM: To study the signal transduction of p38 mitogen activated protein kinase in rat glioma C6 cells after the stimulation of UV radiation. METHODS: Flow cytometry was applied to measure the fraction number changes in the cell cycle phase and to detect whether UV could induce apoptosis of C6 cells. The level and distribution of p38MAPK expression was examined by immunocytochemical method both before and after the UV radiation. RESULTS: Flow cytometry indicated that the numbers of G1 phase fraction of 1,...高等学校骨干教师计划资助 ;; 留学归国人员科研启动基金([1 999] 747号

Effect of Exogenous RGS16 Gene Transfection on Growth of Glioma Cell Line C6

背景与目的:有研究表明野生型p53可以诱导RGS16表达,而RGS16可能与胶质瘤的发生有关。本研究旨在探讨RGS16基因转染对大鼠胶质瘤C6细胞生长的影响。方法:构建真核表达载体pIRES2-EGFP-RGS16,脂质体法转染C6细胞,经G418筛选后荧光显微镜观察细胞中增强型绿色荧光蛋白(enhancedgreenfluorscentprotein,EGFP)的表达,RT-PCR证实RGS16的mRNA表达,免疫细胞化学方法检测细胞中RGS16蛋白表达。最后利用流式细胞仪、生长曲线、平板克隆形成等方法研究RGS16基因对胶质瘤细胞周期、生长及增殖的影响。结果:成功构建了稳定表达RGS16和表达空载体的细胞系C6-RGS16、C6-GFP。C6-RGS16、C6-GFP和C6经流式细胞仪检测S期的细胞比例分别为28.5%、18.9%和14.3%(P<0.05);生长曲线表明C6-RGS16生长的速度明显快于C6-GFP及C6,但其平板克隆形成率分别为12%、25%和25%(P<0.05)。结论:RGS16促进C6细胞周期从G1期向S期过渡,加快细胞生长速度,但是并不促进细胞克隆形成。 【英文摘要】 BACKGROUND & OBJECTIVE: Wild type p53 could induceRGS16 mRNA expression, and RGS16 protein is related with carcinogenesisof glioma. This study was designed to investigate the effects of exogenousRGS16 gene transfection on the growth of rat glioma cell line C6.METHODS : A eukaryotic expression plasmid pIRES2-EGFP-RGS16 wasconstructed, and transfected into C6 cells. The cells were selected by G418.The expression of enhanced green fluorescent protein (EGFP) was observedunder fluorescent microscope, and the exp...军队医药卫生科研基金项目(No.02ma04)~

Effects of transfected HSP70 on p38MAPK signal pathway

目的:探讨热休克蛋白 (HSP70 )在人胶质瘤细胞BT 32 5 p38MAPK信号通路中的作用。方法:用脂质体介导法将hsp70基因导入人胶质瘤细胞BT 32 5中 ,倒置显微镜观察转染细胞的形态学及粘附性变化 ,紫外线照射 30min后 ,采用免疫组化和Western blot方法测定转染前后HSP70的表达水平及照射前后 p38MAPK表达情况。结果:免疫组化和Western blot证实hsp70基因成功转染入BT 32 5中 ,转染细胞受到紫外线照射后 p38MAPK表达减弱。结论:体外转染hsp70基因可抑制紫外线照射后BT 32 5细胞 p38MAPK的表达. Objective To study the role of HSP70 in p38MAPK signal transduction of human glioma cells BT 325.Methods pBBS212 hsp70 gene was transfected into BT 325 cells by lipofectin. The morphological and adhesive changes of the cells were observed under an inverted microscope. The level of HSP70 was measured by immunohistochemistry. Then the transfected cells were put into ultraviolet (UV) for 30 minitues, and expression of p38MAPK and HSP70 were examined by immunohistochemistry and Western blot methods bo...国家自然科学基金资助项目 (30 1 0 0 2 1 8);; 高等学校骨干教师资助计划 (2 0 0 0 - 65 - 66);; 留学归国人员科研启动基金资助项目 (1 999- 747

Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

作者简介: 陈芳育(1978-) , 男, 讲师。E-mail : cfy307@ sohu. com * 通讯作者(Corresponding author) : 陈亮( 1963-) , 男, 教授, 博士生导师, 研究方向: 细胞与分子生物学。E-mail: chenlg@ xmu. edu. cn[中文文摘]运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。[英文文摘]Rice bacterial leaf streak( BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola ( Xooc) is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan (Oryzae sativa L. ) highly resistant to the disease, were infected by"89773-1- 1" strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two- dimensional electrophoresis. It was found that there were thirty- eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was "new". Of the thirty- eight responsive proteins, thirty-three were identified by MALD-I TOF-MS and database searching.Based on the predicted function, we grouped them into four clusters: signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological react ions, including signal recognition and transduction, antioxidant react ion, carbonhydrate metabolism, cel-l wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduct ion and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis- related(PR) proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistan-t related genes in rice to BLS.生物农药与化学生物学教育部重点实验室( 福建农林大学) 开放课题基金项目( KF0411

Construction of the eukaryotic expression vector pIRES2-EGFP-Axin and its expression in glioma cells

目的:构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞系C6中进行表达。方法:用PCR的方法扩增Axin基因,构建真核表达载体pIRES2EGFPAxin,经NheI及SalI双酶切鉴定并测序。通过脂质体法转染C6细胞,用荧光显微镜检测细胞中增强型绿色荧光蛋白(EGFP)的表达,用免疫组化染色的方法检测细胞中Axin的表达。结果:构建了真核表达载体pIRES2EGFPAxin,用脂质体法转染神经胶质瘤细胞C6后,经荧光显微镜和免疫组化染色法检测,可见细胞内有EGFP及Axin的表达。结论:成功地构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞C6中表达,为研究Axin对肿瘤的生物学作用以及Axin在肿瘤基因治疗中的应用奠定了基础。国家杰出青年自然科学基金项目(No.30125012);; 军队医药卫生科研基金项目(No.02ma04);; 高等学校骨干教师及归国留学人员科研启动基金(No.1999747

Construction of the eukaryotic expression vector pIRES2-EGFP-Axin and its expression in glioma cells

目的:构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞系C6中进行表达。方法:用PCR的方法扩增Axin基因,构建真核表达载体pIRES2EGFPAxin,经NheI及SalI双酶切鉴定并测序。通过脂质体法转染C6细胞,用荧光显微镜检测细胞中增强型绿色荧光蛋白(EGFP)的表达,用免疫组化染色的方法检测细胞中Axin的表达。结果:构建了真核表达载体pIRES2EGFPAxin,用脂质体法转染神经胶质瘤细胞C6后,经荧光显微镜和免疫组化染色法检测,可见细胞内有EGFP及Axin的表达。结论:成功地构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞C6中表达,为研究Axin对肿瘤的生物学作用以及Axin在肿瘤基因治疗中的应用奠定了基础。 【英文摘要】 AIM: To construct the eukaryotic expression vector pIRES2-EGFP-Axin, and to express Axin in C6 glioma cells. METHODS: The Axin gene was amplified by PCR using pCMV5-HA-Axin as a template, and confirmed by DNA sequencing. The eukaryotic expression vector pIRES2-EGFP-Axin was constructed by introducing Axin DNA fragment into the sites of Nhe I and Sal I of pIRES2-EGFP vector. The plasmid was transfected into the C6 cells using lipofectamine. The expressed EGFP was observed under fluorescent microscope and the...国家杰出青年自然科学基金项目(No.30125012);; 军队医药卫生科研基金项目(No.02ma04);; 高等学校骨干教师及归国留学人员科研启动基金(No.1999747

Expression and relationship of RGS16 and p53 in human glioma

目的研究G蛋白信号调节子16(RGS16)与p53在人胶质瘤中表达及其相关性。方法利用免疫组织化学链霉亲合素生物素过氧化物酶复合物(SABC)法检测42例胶质瘤标本中RGS16与p53的表达。结果RGS16在10例胶质瘤旁正常脑组织有8例神经元阳性但胶质细胞阴性、42例胶质瘤中37例肿瘤细胞阳性,二者差异显著(P0.05);其中,RGS16与p53共同阳性表达11例,共同阴性表达1例,Kappa检验二者表达呈负相一致(P<0.05)。结论RGS16在胶质瘤中高表达而与病理分级无关,推测RGS16可能在胶质瘤发生中起作用。另外,胶质瘤中RGS16与p53表达呈负相关。 【英文摘要】 Objective To study the expression of RGS16 in human glioma and its relationship with p53. Methods The expression of RGS16 and p53 in protein level was studied by immunohistochemistry strept avidin-biotion-peroxidase-complex (SABC) method in 42 samples.Results In 10 normal brain tissues beside the human glioma, 8 cases expressed RGS16 in the neurons,but none in gliocytes; in 42 human gliomas, 37 cases expressed RGS16 in the glioma cells, and the difference between the normal tissue and the glioma was observe...军队医药卫生科研基金资助项目(02ma04);; 国家杰出青年自然科学基金资助项目(30125012);; 高等学校骨干教师及归国留学人员科研启动基金资助项目([1999]747