Fabrication of the materials containing fluorine with low surface free energy and their properties

润湿性是固体表面的重要性质之一，主要由表面的化学组成和微观几何结构共同决定。近年来，随着微纳米技术的飞速发展以及仿生学研究的兴起，人们对在固体上构筑特殊润湿性表面表现出了极大的兴趣。氟原子半径小、电负性大，与碳原子组成共价键时，C-F键长短，键能大，碳骨架外层排列紧密，并且由于F原子间的排斥作用，形成螺旋结构排列，对主链碳原子和碳链骨架形成有效屏蔽作用，故氟碳化合物表现出卓越的低表面能、化学稳定性、耐腐蚀等性能。含氟单体易与结构相似的丙烯酸酯类单体共聚，并且具有低的表面能及表面张力。含氟（甲基）丙烯酸（酯）共聚物具有“三高”(即高耐候性、高耐热性、高稳定性)、“两憎”(憎水、憎油)的特性，在日...Wettability of a solid surface is an important property governed by both the surface energy and the geometric structure. In recent years, much research effort has been devoted to generating special wettabilities, by combining the fabrication of geometric structure and chemical modification. Because of their small size, fluorine atoms can shield a fluorinated carbon atom without any steric stress. ...学位：工学硕士院系专业：材料学院材料科学与工程系_材料物理与化学学号：2072007115003

Study on organochlorine pesticide and PCBs at surface water in Xiamen Harbour

利用GC -ECD对厦门西港 1998年 7月取得的 9个站位表层水体中的 18种有机氯农药 (HCHs ,DDTs等 )和 12种多氯联苯 (PCBs)进行分析。其中有机氯农药的浓度范围 6 .6 0～ 32 .6ng/L(其中HCHs:3.5 1～ 2 7.8ng/L ,均值 8.5 7ng/L ;DDTs:0 .95～ 2 .2 5ng/L ,均值 1.45ng/L) ,多氯联苯的浓度为 0 .0 8～ 1.6 9ng/L ,同国内外其他港口海区相比较 ,其污染程度相对较低。同时 ,对有机氯污染的分析表明 ,发现近年来仍有有机氯的污染输入 ,其农药的使用主要集中在六六六和滴滴涕上 ;多氯联苯和有机氯农药在厦门西港有着相似的分布及来源特征。organochlorine pesticides and 12 polychlorinated biphenyls(PCBs) at the sruface water in Xiamen Harbour were determined. The range of organochlorine pesticides was 6.60～32.60 ng/L, and the PCBs concentrations were 0.08～1.69 ng/L. Compared with the results of other harbor and estuaries, it showed that the contamination for organic chlorine pollutants was more slight than those of others. At the same time, it was found that organochlorine pesticides and PCBs have the similar geological destribution and resources.福建省自然科学基金!项目 (D9810 0 0 3

DNA polymorphism difference between root system and rhizosphere soil arbuscular mycorrhizal fungi of Prunus mume by AFLP analysis

应用巢式PCr并进行梅根系与根围土壤丛枝菌根真菌(ArbuSCulAr MyCOrrIHIzAl fungI,AMf)dnA扩增片段长度多态性(AMPlIfIEd frAgMEnT lEngTH POlyMOrPHISM,AflP)的差异比较,研究梅共生AMf的作用机理。结果表明,从18个梅品种的30个根围土壤样品中有28个样品获得纯化的dnA片段,占样品数93.3%,样品平均多态性位点数为6.5个,nEI’S基因多样性为0.3559±0.1382,SHAnnOn信息指数为0.5299±0.1676。与梅根系AMf dnA多态性比较,根围土壤的平均多态性位点数明显较多;且根系AMf的dnA多态性位点绝大多数存在于土壤AMf的dnA多态性位点中,表明根系内AMf是由土壤AMf发育而来;根系与根围土壤AMf dnA的聚类均与梅品种群、品种关联性不强,表明AMf对宿主梅品种或品种群没有特异的共生关系。DNA polymorphism of arbuscular mycorrhizal fungi(AMF) in root system and those in rhizosphere soil of Prunus mume was analyzed through DNA amplification by nested PCR based on AFLP marker.The results show that the purified DNA can be extracted from 28 soil samples, accounting for 93.3% of totally 30 samples, collected from rhizosphere of 18 cultivars, averaging 6.5 loci for each sample.The average genetic identity was 0.3559±0.1382, Shannon information index was 0.5299±0.1676.The number of loci in rhizosphere soil was much more than that of loci in root sample, and most of loci in root sample could be found in soil sample.It was proved that the AMF in root might be developed from soil AMF.The clustered groups of AMF DNA by AFLP marker both from roots and from soils were not connected with cultivar groups or cultivars of P.mume, and indicated that there was no specific symbiotic relationship between AMF and P.mume.国家自然科学基金(30470006); 厦门市科技项目(3502Z20072010;3502Z20112004)~

The trends and characteristics of organochlorines pollution in surface sediments of Xiamen Western Bay

利用GC ECD对厦门西港 1 998年 7月取得的 8个站位表层沉积物中的 1 8种有机氯农药 (HCHs,DDTs)等和 1 2种多氯联苯 (PCBs)进行分析 .其中有机氯农药的浓度范围ND(未检测出 )— 0 58ng/g(其中HCHs和DDTs的含量分别为ND—0 1 4ng/g与ND— 0 0 6ng/g) ,多氯联苯的浓度为ND— 0 32ng/g ,与 1 986、1 993年的厦门西港的分析结果相比较 ,污染程度明显降低 ;说明近年来的厦门的有机污染得到逐步控制 ,也与有机物随时间推移逐渐详解有关 ,分析表明多氯联苯和有机氯农药在厦门西港有着相似的分布特征 .The contents of 18 organochlorine pesticedes and 12 polychlorinated biphenyls(PCBs) in suface sediments from Xiamen Western Bay were analyzed by GC\|ECD. The range of organochlorine pesticides was ND—0 58?ng/g,and the PCBs were ND—0 32?ng/g.Compared with the results of Xiamen Harbor in 1986 and 1993, it showed that the pollution of organochlorines decreased gradually along the time, in Xiamen Harbor became and the contamination was more slightly than that of other Harbors or Estuaries. At the same time, it was found that the geological distributions of organochlorine pesticides and PCBs, also the HCHs and DDTs were similar to each other, which indicated that the polltion was cansed by a same source

Evaluation and Fate of the Organic Chlorine Pesticides at the Waters in Jiulong River Estuary

1999 0 6,对九龙江口 1 5个站位的表层水 ,1 3个站位的间隙水进行了 1 8种有机氯农药的测定结果表明 ,有机氯农药总含量在表层水中的浓度范围为 51 3～ 2 4 79ng L ;在间隙水中的浓度范围是 2 66～ 33355ng L .对不同有机氯的含量在各站位的分布特征进行了探讨 ,发现Methoxychlor(甲氧滴涕 ) ,EndosulfanSulfate(硫酸硫丹 ) ,Endrinaldehyde(乙醛异狄氏剂 )以及EndosulfanII(硫丹 ) ,Dieldrin(狄氏剂 ) ,Deta HCH和Beta HCH 7种有机氯农药在 1 8种有机氯农药中都占主要部分 ;九龙江口的六六六的含量顺序 :β >δ α >γ ;对于滴滴涕 ,表层水中的含量 :DDE DDD >DDT ;间隙水中的含量 :DDE DDT >DDD ,二者DDE的含量都在总DDTs的 50 %以上 ,说明环境中的DDTs主要降解为DDE ;九龙江口有机氯农药随着盐度梯度 ,在河口中呈去除趋势 ;且间隙水中有机氯农药比表层水中的浓度高 ,说明其倾向于吸附在沉积物颗粒上 ,其浓度差使得有机污染物可能通过再悬浮等过程从底层向上层迁移 .九龙江口的有机氯农药污染与其他港湾相比 ,污染水平相当 ,部分站位水质有机氯农药 (HCHs和DDTs)超过国家一类水质的标准 .Organochlorine Pesticides at the water(surface water and porewater) in Jiulong River Estuary were determined. The range of organochlorine pesticides at surface water was 51.3～2479ng/L, and the concentration in porewater was 266～33355ng/L. The results showed that the organochlorine pesticides were removed in the estuary and the sediments were the reservior of the organochlorine pesticides. Compared with the results of other harbor and estuaries , it showed that the contamination for organic chlorine pollutants in Jiulong River Estuary was similar to those of others. At the same time,the risk of organochlorine pesticides in the estuary was evaluated.1998年教育部重点项目、基金委资助留学人员短期回国工作讲学专项基金!项目联合资助 (4 9910 76 0 74

含氟硅低表面能共聚物的制备与表征

将丙烯酸六氟丁酯和γ-甲基丙烯酰氧基丙基三甲氧基硅烷与(甲基)丙烯酸(酯)进行共聚,合成含氟硅共聚物。用红外光谱(FT-IR)、X射线光电子能谱(XPS)和热重分析(TGA)对共聚物的结构和性能进行表征,并测试了共聚物膜的吸水率及对水和油的接触角。XPS结果表明,共聚物膜表面的F和Si元素相对物质的量明显高于150 nm深处,同时共聚物膜对水和油的静态接触角分别达到110°和80°以上,说明F、Si元素自组装迁移,形成了低表面能表面;TGA研究结果表明,共聚物膜的热分解温度从195℃提高到226℃,升温过程中分解速率明显降低。该含氟硅共聚物具有良好的热稳定性、低的吸水率和良好的疏水防污性

诱导型一氧化氮合酶和血管内皮生长因子在舌癌细胞株中的表达

【目的】探讨诱导型一氧化氮合酶（iNOS）和血管内皮生长因子（VEGF）在舌鳞癌细胞株Tca8113中的表达,为进一步研究iNOS和VEGF在舌鳞癌肿瘤血管生成中的作用提供实验基础。【方法】采用逆转录-聚合酶链反应（RT-PCR）法和SP免疫组化方法,对舌鳞癌细胞株Tca8113中iNOS和VEGF mRNA和蛋白表达情况进行检测。【结果】RT-PCR检测到舌鳞癌Tca8113细胞株中iNOS／VEGF mRNA条带明显,SP免疫组化检测到iNOS／VEGF蛋白在舌癌细胞胞浆中呈强阳性表达。【结论】iNOS／VEGF mRNA和蛋白在舌鳞癌Tca8113细胞株中呈高水平表达

TT病毒与肝炎关系的临床流行病学研究

目的　对闽南地区各种肝炎患者、健康体检者、义务献血员和肝癌患者共480例从临床流行病学角度探讨TT病毒(TTV)的致病性及其与各种肝炎的关系。方法　采用巢式PCR检测血清TTVDNA、ELISA检测血清抗HAVIgM、HBsAg、抗HBcIgM、抗HCVIgG、抗HEVIgG,用EPIINFO60软件进行统计分析。结果　480名研究对象中TTVDNA的总检出率为23.96%。各种肝炎患者的TTV总阳性率为2394%,肝癌患者的TTV阳性率为2069%,而健康者的TTV阳性率为2484%,义务献血员的阳性率为3000%,均未见明显差别。从临床类型看,急性肝炎、慢性肝炎和重症肝炎的TTV阳性率都在25%左右;从病原类型看,非甲～戊型肝炎的TTV阳性率为2619%,并未见与相应健康者的2523%阳性率的差别;除HCV由于感染率太低而无法分析外,HAV、HBV、HEV阳性肝炎患者间TTV的阳性率分别为2000%、2314%、2179%,未见TTV与这些已知肝炎病毒的明显相关。对一个时期内的全部135例住院肝炎患者及153名健康者进行肝炎病原分析,HAV、HBV、HEV在肝炎患者中的阳性率都要明显高于健康人(P=00142),而TTV在肝炎患者中的阳性率与健康人没有明显差别(P=06021);对病毒的单独致病性进行分析,HAV、HBV、HEV在非重叠感染的肝炎患者中的阳性率都要明显高于健康人(P=00037),而TTV在非甲～戊型肝炎患者中的

Y型聚乙二醇干扰素琢-2b注射液治疗HCV基因2/3型慢性丙型肝炎患者疗效和安全性的多中心随机对照试验研究

目的以标准剂量的聚乙二醇干扰素(Peg IFN)α-2a联合利巴韦林作为阳性对照,评价新型试验药物Y型Peg IFNα-2b注射液联合利巴韦林治疗2型/3型慢性丙型肝炎(CHC)患者的疗效和安全性。方法采用多中心、随机开放、阳性药对照的Ⅲ期临床试验,筛选符合要求的2型/3型CHC患者,按照2:1的比例随机分配到Y型Peg IFNα-2b组和Peg IFNα-2a组,同时口服利巴韦林,疗程24 w,停药随访24 w。采用Abbott Real Time HCV Genotype II检测HCV基因型,采用Cobas Taq Man实时定量PCR法检测血清HCV RNA水平。详细记录不良事件。主要疗效指标为持续病毒学应答(SVR),并进行非劣效检验。结果本试验实际入组2型/3型CHC患者255例,实际治疗241例。全分析集(FAS)数据显示,158例试验组和83例对照组患者SVR分别为85.4%(95%CI 79.94%~90.94%)和79.5%(95%CI 70.84%~88.20%,P=0.2402);对符合方案分析集(PPS)人群分析显示,试验组和对照组患者SVR分别为87.9%(95%CI 82.45%~93.27%)和85.9%(95%CI 77.82%~94.01%,P=0.7060),率差的95%可置信区间均符合非劣效标准;对PPS人群分析显示,85.8%受试者获得了早期病毒学应答(RVR),RVR的阳性预测值为90.1%;试验组和对照组不良事件发生率相似,分别为95.6%和95.2%,严重不良事件发生率分别为3.8%和3.6%。结论应用Peg IFNα联合利巴韦林治疗2型/3型CHC患者,新型试验药物Y型Peg IFNα-2b具有与对照药物Peg IFNα-2a相似的疗效和安全性。国家科技部“十二五”重大专项(编号:2012ZX10002-003)；“重大新药创制”十二五科技重大专项(编号:2012ZX09303019)