Over-expression of PC-1 Gene Increases Survival Ability of C4-2B Prostate Cancer Cell Line

建立稳定表达外源PC-1基因的人前列腺癌骨转移C4-2B细胞模型,初步探讨PC-1基因表达对前列腺癌发展的影响.通过脂质体介导的方法,将融合PC-1基因的真核表达载体pcDNA3·1-PC-1稳定转染C4-2B细胞,Western印迹和RT-PCR技术,分别从蛋白水平和RNA水平确定外源PC-1基因表达.MTT和软琼脂集落形成能力等一系列方法,研究PC-1基因的功能,RT-PCR和实时定量PCR检测前列腺癌发生发展相关基因表达的变化.结果表明,PC-1基因的高表达能够诱导雄激素受体(AR)调控基因和一系列重要的信号通路成员基因PSA、PSMA、NKX3·1、Jagged1、EphA3、SGEF和NOTCH3等表达发生变化.实验结果初步证明,PC-1基因表达在晚期前列腺癌中,以及在雄激素非依赖的转变中可以发挥作用,PC-1基因表达可调控一些重要信号通路.对PC-1基因功能深入研究将有可能为发现新的前列腺癌的诊断治疗分子靶标提供线索.To investigate the potential role of PC-1 gene over-expression on prostate cancer progression, a metastasis and androgen independent prostate cancer cell line C4-2B was used, cell clones constitutively expressing PC-1, and their mock-transfected counterparts were constructed. The expression of ectopic PC-1 was analyzed by Western blot and RT-PCR assay. MTT and colony anchor-independent growth in soft agar were performed to evaluate the effects of PC-1 gene expression on prostate cancer progression. RT-PCR and real-time PCR were used to analyse the expression of differential genes between C4-2B-PC-1 and C4-2B-neo cell lines. Our results showed that stable expression PC-1 induced transcription change of some genes, including PSA,PSMA,NKX3.1,Jagged1, EphA3, SGEF and NOTCH3, which were the androgen receptor, regulated genes and important signal transduction pathway molecules. These observations indicated that PC-1 over-expression might play important role in advanced prostate cancer progression, promote prostate cancer cell androgen independent growth, and may regulate multiple signal transduction pathways. Further study of PC-1 gene function may provide clue to discover new molecular targets for prostate cancer diagnosis and treatment.国家自然科学基金资助(No.30070296)~

Study of the Modified Electrolytic Manganese Dioxide

用电解法制备一种掺杂铋的改性二氧化锰材料（Ｂｉ－ＥＭＤ）．通过ｘ射线衍射及光电子能谱分析，Ｂｉ－ＥＭＤ属于γ－ＭｎＯ＿２，Ｂｉ以Ｂｉ＿２Ｏ＿３形式存在于改性材料中，且材料中Ｂｉ含量随电解时Ｂｉ￣（３＋）浓度增加而增加．当电解液中Ｂｉ￣（３＋）浓度为０．００８ｍｏｌ·Ｌ￣（－１）时，材料中Ｂｉ含量高达１４．３％（重量百分比）．用Ｂｉ－ＥＭＤ装配成ＡＡ型Ｚｎ／ＭｎＯ＿２试验电池．通过循环寿命实验，Ｂｉ－ＥＭＤ表现出良好的可逆性．A modified electrolytic manganese dioside doped with Bi(Bi-EMD)wasprepared and examined by XRD and XPS.It is predominantly γ-mnO_2 and the Bi exists as Bi_2O_3 inthe modified material.The content of Bi in Bi-EMDs increased with the increasement of Bi￣(3+)concentration in electrolyte,and reached14.3(wt)% from the bath of 0.008 mol·1￣(-1)Bi￣(3+)+ used.Some Zn /Bi-EMD prototype batteries of AA size were assembled,and which cycle life tests showedthat the Bi-EMD has better reversibility.作者联系地址：哈尔滨工业大学应用化学系Author's Address: Department of Applied Chemistry,Harbin Institute of Technology,Harbin,15000

Electrochemical Properties of Polymeric Gel Electrolyte with GBL/EC as Plasticizers

以GBL/EC复配体系为增塑剂, PVDF HFP和PMMA为聚合物基体制备胶态聚合物电解质. 研究聚合物电解质的离子传输特性和电化学稳定性. 实验表明,室温离子电导率达到 1. 2mS·cm-1, 电化学稳定窗口在 4. 5V以上. 以GBL/EC增塑聚合物电解质与表面经修饰的锂金属电极组成锂金属聚合物电池,其电极稳定性较好,充放电循环寿命得到很大的提高.Polymeric gel electrolytes (GPE) based on PMMA and PVDF-HFP were prepared using the binary mixtures of γ-butyrolactone (GBL) and ethylene carbonate (EC) as plasticizers. The ionic transport properties and electrochemical stability were studied. The room temperature conductivity reached 1.2 mS·cm~(-1), electrochemical stability window exceeded 4.5V. The interfacial stability between GPE and lithium electrode was also investigated. The lithium metal polymer battery (LMPB) prepared with the GPE showed better charge and discharge life cycle when the the lithium metal electrode was treated with 1,4-dioxane.作者联系地址：哈尔滨工业大学理学院应用化学系,哈尔滨工业大学理学院应用化学系,广东省江门三捷电池实业有限公司研究中心,广东省江门三捷电池实业有限公司研究中心,哈尔滨工业大学理学院应用化学系,哈尔滨工业大学理学院应用化学系 黑龙江哈尔滨150001广东省江门三捷电池实业有限公司研究中心广东江门529000 ,黑龙江哈尔滨150001 ,广东江门529000 ,广东江门529000 ,黑龙江哈尔滨150001 ,黑龙江哈尔滨150001Author's Address: 1. Department of Applied Chemistry, Harbin Institute of Technology,Harbin 150001,China,2.Research center of JJJ Battery Co. LTD.,Jiangmen 529000, Chin

Molecular Cloning and Characterization of a Putative Promoter Region of mPC-1 Gene Homologous to hPC-1

为了鉴定鼠mPC-1基因表达的调控元件,克隆并分析了该基因的启动子.构建了一系列mPC-1基因启动子的截短序列.通过荧光素酶报道基因,分析了它们在前列腺癌细胞和其它细胞中的表达.结果表明,在AR阳性细胞系中,mPC-1基因启动子活性远远高于SV40和p61-PSA启动子,mPC-1基因启动子599bp至449bp可能含有一个负调控元件;mPC-11.1kb启动子控制的表达主要在前列腺癌细胞系中;雄激素可调控mPC-11.1kb启动子表达.mPC-11.1kb序列是一个有前列腺癌细胞特异性和较强的启动子,经过进一步的修饰有可能作为一种有用的前列腺癌基因治疗元件.To identify the regulatory region that are responsible for the expression of mPC-1,we have isolated and characterized the mPC-1 gene promoter.Sequence analysis of the mPC-1 5'-flanking region and a series of truncated constructs were performed,which were transiently transfected into the prostate cancer cell lines and non-prostate cancer cell lines and analyzed through Dual-luciferase reporter assay system.The relative activity of mPC-1 gene promoter was by far higher than pGL3-control containing SV40 promoter and enhancer and p61-PSA containing hPSA 6 kb promoter in AR(androgen receptor,AR)-positive prostate cancer cell lines.The region from 599 bp to 449 bp of mPC-1 promoter might contain a negative regulatory element.The expression of mPC-1 1.1 kb fragment is mainly restricted into prostate cancer cell lines.The relative activity of mPC-1 1.1 kb 5'-flanking region was regulated by androgen.The results demonstrated that the 1.1 kb fragment of mPC-1 5'-flanking region was relatively strong and prostate cancer cell specific promoter region.The 1.1 kb promoter of mPC-1 gene might be well suited to prostate cancer gene therapy if the promoter was properly modified.国家高技术研究发展计划(863计划)(No.2002AA223061);; 国家自然科学基金(No.30070296)资助~

2000–2010年中国典型陆地生态系统实际蒸散量和水分利用效率数据集

蒸散是陆地生态系统水分循环和能量平衡的关键过程,水分利用效率是反映生态系统碳水循环间耦合关系的重要指标,二者在生态学、农学、水文学、气候学等多个学科中均具有重要的应用价值。涡度相关法被认为是现今唯一能直接测量生物圈与大气间物质与能量交换通量的标准方法,已成为生态系统尺度碳水交换通量观测的主要方法。本文通过整合中国陆地生态系统通量观测联盟(China FLUX)的长期观测数据和中国区域其他观测站点基于涡度相关法发表的文献数据,构建了一套中国典型陆地生态系统实际蒸散量和水分利用效率数据集。本数据集共有实际蒸散量数据记录143条、水分利用效率数据记录96条,涉及5种生态系统类型45个生态系统,时间跨度为2000–2010年。本数据集可以为陆地生态系统碳水循环、生态系统管理和评估、全球变化等相关领域的研究提供数据支持