AC-AC Converter With High Frequency Link

电力电子变换技术在过去六十年里已经取得较大发展，人们在AC/DC、DC/DC、DC/AC等领域内的中、小功率变换器研究已经取得了显著成就。然而，对AC/AC变换器研究仅局限于可控硅变换器、矩阵变换器和交流斩波器等交流电网与负载无电气隔离领域。目前国内外对于AC/AC变换技术的研究仍处于初级阶段，寻找一种结构简洁、控制模式方便的主拓扑具有很重要应用意义。 本文通过了解各种典型和新兴的交流变换器主拓扑结构及其性能特点，对高频连接型全桥桥式变换器的电路拓扑、控制原理等进行了深入研究，设计出一款可运行恒压、恒流两种工作模式的AC-AC变换器。经过仿真分析、实际软硬件电路设计等步骤后完成了该变换器样机...In the past 60 years, power electronic conversion technology has achieved great development. People study in AC/DC, DC/DC, DC/AC fields, in medium and small power converter, has made remarkable achievements. However, the study of AC/AC converter is very little, only to do some research about the SCR converter, matrix converter and AC chopper, there is no electrical isolation between their input an...学位：工学硕士院系专业：物理与机电工程学院_机械设计及理论学号：1992012115272

从“个体”概念看自然选择理论的抽象化与扩展

上世纪西方学界关于自然选择单位与层次的讨论,可视为一个更大的进程,即自然选择理论的抽象化与扩展的开端。本文以\"个体\"概念的演变为线索,描绘出关于该进程的一幅完整图景,并整理出三个维度,其一是自然选择从有机体层次向其它组织层次的扩展,其间奠定了自然选择理论抽象化的两个基本框架——经典进路和复制子进路;其二是从共时结构到历时结构的扩展;其三是选择对象从典范实体向非典范实体的扩展。本文指出,复制子进路具有启发价值,但经典进路更适合构建一般性的抽象框架,未来进化理论发展的方向应当是不同维度的进一步综合,以及进化生物学与其它生物学学科的\"扩展综合\"。国家社科基金重大项目“生物哲学重要问题研究”(项目编号:14ZDB171

Studies on Transformation of Tomato with Vaccine of Recombinant M2 Peptide of AIV and Keratinocyte Growth Factor

禽流感(AvianInfluenza,AI)是由正粘病毒科A型流感病毒引起的一种以侵害呼吸系统为主的高度接触性传染病。其高致病力毒株可引起90%以上的死亡率，每次爆发都给养禽业带来巨大的经济损失，因此被国际兽疫局列为Ⅰ类烈性传染病。禽流感病毒M2蛋白是由97个氨基酸组成的寡四聚体，与HA和NA基因相比，M2基因具有高度保守性。因此，与其他禽流感多肽疫苗相比，M2蛋白具有交叉保护能力的“通用”流感疫苗研究的候选抗原的优势。 人角质细胞生长因子(Keratinocytegrowthfactor,KGF)是由163个氨基酸残基组成的26-28KD单链多肽。KGF对皮肤创面的上皮细胞具有促进增殖、促...Avian influenza virus (AIV) infect almost all wild birds and domestic poultries and then decimate poultries. Severe economic loss of the poultry industry was brought because of the AIV prevalence. The M2 protein of AIV is a kind of cross-protection constructed by 97 amino acids , and a high evolutionary conservative protein in different subtypes. Because AIV infects animals by the respiratory and ...学位：理学硕士院系专业：生命科学学院生物学系_发育生物学学号：2012005130204

Studies on Construction of Plant Expression Vector of Recombinant LBM2eHBc+ Gene and Tomato Transformation

禽流感病毒一直是家禽和家畜健康养殖的巨大威胁,甚至威胁着人类的健康,人们也一直在研究各种形式的疫苗来应对禽流感病毒的威胁.番茄作为生物反应器应用于动物疫苗生产具有独特的优点,本研究优化了番茄子叶的再生体系,同时构建了lbM2EHbC+融合基因的植物表达载体并对番茄进行了遗传转化.结果表明在玉米素(zT)浓度为0.5 Mg/l时番茄子叶外植体的芽再生率达到93.33%;测序结果证明植物表达载体PbI121-lbM2EHbC+构建成功;利用农杆菌介导的转化方法获得再生抗性苗34株,经PCr检测,阳性率为75.0%,本研究结果为进一步开发禽流感口服疫苗奠定了基础.Avian influenza virus(AIV) infect almost all wild bird and domestic poultries and then decimate poultries,even the threat of AIV overhangs human.It also has been researching a variety of vaccines to combat the AIV threat.Tomato as a bioreactor used in animal vaccine production has a unique advantage.This study optimized the regeneration system of tomato cotyledon,while construct LBM2eHBc + gene plant expression vector,and then carried out the genetic transformation of tomato.The results show that the bud regeneration rates of tomato reach the highest 93.33% when MS medium supplemented 0.5 mg/L Zt+ 1 mg/L IAA;sequence results show that plant expression vector pBI121-LBM2eHBc + constructed successfully.A total of 34 putative transgenic tomato plants were obtained by using of Agrobacterium-mediated transformation method,and the positive rate was 75.0% by PCR detection.The results laid a foundation for the further development of oral AIV vaccines.福建省科技厅重大项目子课题(2006NZ0003-2);福建省教育厅A类科技项目(JA09168

Expression and Construction of Yeast Expression Vector of LBM2eHBc+ gene in P.pastoris

禽流感病毒一直是家禽和家畜健康养殖的巨大威胁,之前的研究表明,原核系统中表达的lTb和M2EHbC+融合基因的产物能有效诱导免疫动物对禽流感病毒M2E多肽产生特异性的粘膜免疫应答.酵母作为生物反应器应用于生物制品生产具有独特的优点.本研究构建了PPIC9k-lbM2EHbC+酵母表达载体并对酵母gS115进行了遗传转化,甲醇诱导表达结果表明lbM2EHbC+融合基因在酵母细胞中得到表达,表达出的融合蛋白能够被M2抗体识别.Avian influenza virus(AIV) has been a constant threat to the healthy development of livestock and poultry breeding industry.Previous research has shown that the products of LTB and M2eHBc+ fused gene expressed in prokaryotic cells can effectively induce mice mucosal immune responses against M2e epitope.P.pastoris yeast has a unique advantage as a bioreactor used in the production of biological products.LBM2eHBc+ fused gene fragment was obtained by PCR,then inserted into pPIC9K plasmid which contains methanol promoter and yeast signal peptide to construct yeast expression vector pPIC9K-LBM2eHBc+.The recombinant vector was transformed into P.pastoris GS115.The methanol induction result has indicated that the LBM2eHBc+ fusion gene can be efficiently expressed in GS115 cell and the expressing protein has the specific binding activity to M2 antibody by Western blotting analysis..福建省科技厅重大项目子课题(2006NZ0003-2);福建省教育厅A类科技项目(JA09168

The Effects of Different Lights and Gibberellin on Establishment of Parasitism between Dodder and Its Hosts

首次使用lEd作为光源,研究不同光照条件及gA3对菟丝子(CuSCuTA SPP.)弯钩打开、缠绕发生与吸器形成的影响。结果表明,光照信号作为一个必要条件参与了菟丝子对寄主的识别及缠绕发生的调控,而化学信号可能起到一定的促进作用;gA3参与了对菟丝子缠绕发生的调控,但对弯钩打开没有明显的作用。除了典型的光敏色素作用外,还有另一类光反应(HEr)参与了上述过程,这类光反应可由879 nM远红光引发,证明菟丝子存在HEr,还有Pfr向Pr的暗转化过程,在缠绕发生过程中光敏色素和隐花色素发生相互作用。The effects of different light treatments and GA3 on hook opening,twining and parasitism of Cuscuta australis were studied using LED as light sources for the first time.The results showed that light was a necessary factor for dodders to parasitize the hosts successfully and chemical signals might facilitate host recognition and twining.GA3 involved in controlling twining response,but no distinct effect on hook opening.Furthermore,besides typical(phytochrome reaction,another photoreaction called HER was involved in these processes,which could be caused by 879 nm far red light.So it demonstrated directly that there was not only HER in dodders,but also dark conversion from Pfr to Pr,and there were mutual interaction of phytochromes and cryptochromes in twining.教育部重点项目(101102)资

Study on Tomato Transformation with Human Keratinocyte Growth Factor-1

人角质细胞生长因子在组织的损伤修复中起着重要的作用.以番茄子叶为外植体,通过根癌农杆菌介导法,将人kgf-1导入番茄中,共获得12株独立的抗性转化植株,PCr和dnA斑点杂交结果表明:kgf-1基因整合入番茄基因组中,为获得植物源表达的kgf-1蛋白打下了基础.Keratinocyte growth factor plays an important role in the tissue damage repair.Using sterile tomato cotyledons as explants,the human KGF-1 gene had been introduced into the tomato genome by Agrobacterium-mediated transformation method.As a result,a total of 12 independent putative transgenic tomato plantlets were obtained.The results of PCR and DNA dot-blot hybridization showed that KGF-1 gene was integrated into the tomato genome.This work has laid a foundation for further studies on the development of KGF-1 proteins expressed in tomatoes.福建省自然科学基金(2010J01240

Alienation of the Relationship Between Master and Apprentice in the Scientific Society: How to Reconstruct Their Relationship in the Era of Big Science

伴随小科学向大科学演变,科研劳动成为社会化大生产的一部分,任务导向的科研契约关系日益冲击师徒之间基于兴趣导向的自主探索,使得科研领域传统的师徒关系在三个方面呈现异化的趋势:兴趣导向的选择关系异化为任务导向的选择关系、导学关系异化为等级制主导的行政关系、合作关系异化为利益驱使的雇佣关系。师徒关系的异化抑制了师徒双方科研自主性的发挥,阻碍了师徒之间科学精神的传承,不仅可能造成学生能力素质的畸形发展,而且在更深层次上影响了科学谱系的延续和科学世代的繁衍。大科学时代师徒关系的重建,关键是要处理好师傅的角色定位以及社会对科学教育的需求定位。With the evolution of the small science to big science, scientifi c research has become a part of socialized mass production.The task-oriented contractual relationship in scientifi c research increasingly affects the interest-orientated autonomous exploration of master and apprentice, which leads to the alienation of the traditional relationship between master and apprentice in three aspects: The interest-oriented preference has become task-oriented; traditional teaching and learning have been administered with a hierarchy, the master as the superior and the apprentice as the inferior; previous cooperative relations have become profit-driven.The alienation of the relationship between master and apprentice inhibits their autonomy in scientifi c research, hinders the inheritance of the scientifi c spirit, which may not only cause the abnormal development of the apprentice's ability, but also affects the continuation of scientifi c lineage and scientifi c generation reproduction in a deep level.The key to reconstructing the relationship between master and apprentice in the era of big science is to defi ne the master's role appropriately and satisfy the need of the society for scientifi c education.依托全军高层次创新型科技人才研修平台深化“钱学森创新拓展班”教学改革研究与实践;国防科技大学“十二五”本科教育教学重点课题(项目编号:U2012011); 继续教育中MOOC教学模式应用研究与实践(课题批准编号:XJK014BCR001

并行计算集群在经济学实验室中的应用

现代经济学研究的最新特征对经济研究中所使用的计算方法与速度提出了更高的要求,并行计算方法的引入成功地解决了许多传统PC难以解决的运算问题。介绍了并行计算在经济学研究中的作用,并结合国外研究型大学经济学实验室并行计算集群建设的经验,提出在我国研究型大学经济学实验室中建设高性能计算集群的必要性与可行性。最后,结合实际建设经验提出建设方案及使用效果评价