The role of RANKL in the regulation of NFATc1, c-Src and RANK gene expression in osteoclast-like cells

目的：体外实验观察不同浓度核因子κB受体活化因子配体（RANKL）对大鼠破骨细胞活化T细胞核因子1(NFATc1)、原癌基因c-Src、核因子κB受体活化因子（RANK）mRNA表达的影响；研究破骨细胞中活化T细胞核因子1、原癌基因c-src和核因子κB受体活化因子在骨质疏松中的发病过程中基因的表达是否与核因子κB受体活化因子配体的浓度存在相关性。 方法：实验采用5周龄SD大鼠获取骨髓基质细胞（BMSC），加入集落刺激因子（M-CSF）及RANKL体外诱导培养破骨细胞，在实验过程中每组分别加入低、中、高（50ng/ml、75ng/ml、100ng/ml）3种浓度的RANKL及均加入M-C...Background and aims The aim of this study was explored the relationships of different concentration of Receptor Activator of NF-kB Ligand (RANKL) and the gene expression of NFATc1, c-Src and RANK in vitro ,in order to find the relationships of the pathogenesis of osteoporosis and gene expression of NFATc1, c-Src and RANK with the concentration of RANKL. Methods We havest bone marrow stromal cel...学位：医学硕士院系专业：医学院_内科学学号：2452011115340

低频脉冲超声对骨关节炎中软骨细胞的影响

超声被广泛应用于临床的诊断和治疗,但是临床上低频脉冲超声(lIPu)的应用却非常有限。在实验中,lIPu的应用却是很广泛。在许多的动物实验中证实,lIPu对许多结缔组织疾病有一定疗效。骨关节炎(OA)是以关节软骨细胞、细胞外基质、软骨下骨等合成与分解代谢失衡,关节软骨损坏为特征的全关节疾病。国家自然基金资助(81272168

Effects of Morinda officinalis-containing serum on the m RNA expression of C-FOS and Cbfa1 in osteoblast and osteoclast co-cultured system

目的观察不同浓度巴戟天含药血清对体外培养成骨-破骨细胞共育体系中原癌基因(C-fOS)、核心结合因子(CbfA)1 MrnA表达的影响。方法提取24 H内新生Sd乳鼠颅盖骨分离培养成骨细胞,采用5周龄Sd大鼠双侧股骨、胫骨的骨髓基质细胞,加入集落细胞刺激因子(M-CSf)和细胞核因子κb受体活化因子配体(rAnkl)诱导培养破骨细胞。采用碱性磷酸酶(AlP)染色鉴定成骨细胞,抗酒石酸酸性磷酸酶(TrAP)染色、骨吸收陷窝甲苯胺蓝染色、电镜扫描等鉴定破骨细胞,体外建立成骨-破骨细胞共育体系,设置低、中、高三种浓度巴戟天含药血清组和不含药血清组,干预3 d后提取各组总rnA,应用逆转录-聚合酶链反应(rT-PCr)方法测定各组C-fOS、CbfA1 MrnA表达量。结果不同浓度巴戟天含药大鼠血清对成骨-破骨细胞共育体系C-fOS有抑制作用,对CbfA1 MrnA的表达有促进作用。高浓度含药血清组两者的表达差异显著(P<0.05,P<0.000 1)。结论巴戟天含药血清可抑制成骨-破骨细胞共育体系C-fOS的表达,促进CbfA1 MrnA的表达,从而达到降低破骨细胞分化成熟及骨吸收活性,促进骨形成。Objective To observe the effects of the serum of Morinda officinalis( RMO) on the expression of C-FOS and core binding factor Alpha1( Cbfa1) in osteoblast and osteoclast co-cultured system.Methods Osteoblasts were separated from the cranium of 24 hours newborn SD rat.Bone marrow cells were harvested from bilateral femora and tibiae of five weeks old SD rat,and M-CSF and RANKL were used to induce osteoclast formation.Osteoblast cells were confirmed by alkaline phosphatase( ALP) stain,osteoclast cells were confirmed by tartrate resistant acidphos phatase( TRAP) stain,Toluidine blue stain and bone resorption assay.Osteoblast and osteoclast co-cultured system was established in vitro.Low,middle,high concentrations of serum RMO and control groups were set.Total RNA was extracted after intervention 3 days,C-FOS and Cbfa1 mRNA expression were measured by real-time PCR.Results Different concentrations serum of RMO had inhibitory effect on the expression of C-FOS and enhance the mRNA expression of Cbfa1 mRNA,and the function on both indicated a statistically significant difference at high concentration( P<0.05,P<0.000 1).Conclusions The serum of RMO could down-regulate the expression of C-FOS and up-regulate Cbfa1 mRNA in osteoblast and osteoclast co-cultured system,consequently reduce osteoclast differentiation and activity of bone resorption,enhance bone formation.国家自然科学基金资助项目(No.81272168); 福建省医学创新课题资助项目(No.2012-CXB-32

Automatic Web Information Extraction Based on Maximal and Frenquent Equivalence Classes

在定义模板的基础上,提出了页面创建模型。该模型描述了如何使用模板将来自于后台数据库的值编码生成页面。基于这个模型,设计了一个基于最大频繁等价类的抽取算法EBMFEC,通过分析给定的数据导向型页面的终端符号的出现情况,找出最大频繁等价类,并推导出用于生成页面的未知模板。然后使用推导出的模板,从输入页面中提取出相关信息。在大量实际HTML页面上的实验证明,EBMFEC在大部分情况下都可以从给定页面中推导出模板,并正确抽取出数据信息。A novel approach based on MFEC(Maximal and Frenquent Equivalence Classes)is proposed to solve the problem of automatically extracting data from data-intensive Web pages.A template is defined and a model of page creation is proposed to describe how values are encoded into pages using the defined template. We present an algorithm,EBMFEC that takes,as input,a set of template-generated pages,analyzes the page-tokens of given pages to discover MFEC,deduces the unknown template used to generate the pages and extracts,as output,the values encoded in the pages. Experiments on a large number of HTML pages indicate that our algorithm correctly extracts data in most cases and the results are also provided.国家自然科学基金(50474033);; 福建省自然科学基金(A0310008);; 福建省重点科技项目(2003H043)

基于主客体识别的自愈合功能材料

近年来,自愈合材料的研究引起了广泛的兴趣,已报道的自愈合材料大多为结构性自愈合材料。具有特殊光、电等性能的功能性自愈合材料尚处于初期研究阶段。本课题组基于环糊精自组装的研究发展了一系列自愈合功能材料。1)自愈合缓冲材料。二茂铁改性的聚乙烯亚胺和环糊精修饰的多官能脂肪酸形成的超分子材料具有良好的缓冲性能,愈合前后材料的缓冲性能基本相同。2)自愈合导电材料。发展了两类自愈合导电材料:金刚烷改性的聚乙烯亚胺和丙烯酸乙二醇酯分别与环糊精改性的碳纳米管形成的复合材料。该系列自愈合材料表现出优异的弹性、机械性能和导电性能。3)自愈合多功能涂料。基于课题组构筑自愈合功能材料的理念,制备了含有Fe_3O_4和..

Fabrication of Gold Nanoparticles Array Film as Surface-Enhanced Raman Spectroscopy Substrate

表面增强拉曼光谱(SERS)是一种灵敏度很高的分析技术,可以给出分子水平的光谱信息,常被用于痕量分析检测。金纳米颗粒(AuNPs)由于其可控制备、独特的光学特性,是最常用的SERS基底材料之一。然而,如何经济、高效地制备出一种性质稳定、耐保存的拉曼基底材料,仍是亟待解决的问题。文中通过静电纺丝工艺,将修饰了beta-环糊精的AuNPs聚合液制备成静电纺丝薄膜;将此薄膜用作SERS基底材料,以罗丹明6G作为探针分子,获得了优异的SERS效果,增强因子约为105,检测限达到10-6级以下,且基底材料的重复性优越

Design and experimental study of space continuous robots applied to space non-cooperative target capture

Space capture actuators face problems such as insufficient flexibility and electrical components that are vulnerable to extreme space environments. To address these problems, a centralized-driven flexible continuous robot based on a multiple scissor mechanism units is proposed in this study. The continuous robot body is composed of two scissor mechanism units coupled in series, and the base container&rsquo;s three motors to drive the robot. The two scissor mechanism units ensure a wide range of flexible operations and the light weight of the robot. The centralized drive with three motors not only reduces the number of driving sources, but also ensures temperature control and protection of electrical components in the space environment. The kinematics and dynamics of the robot are analyzed, and the workspace and deformation performance of the robot are verified through experiments. Compared with other continuous robots, the proposed continuous robot retains the characteristics of continuous robots in a wide range of flexible operations. At the same time, the configuration is light and a small number of driving sources are used, which is suitable for extreme temperatures, vacuum, radiation, and strict resource-constrained environments in space.</p

论草牧业的理论体系及其实践

"草牧业"是在传统畜牧业和草业基础上提升的新型生态草畜产业,包含饲草料生产、加工以及畜禽养殖(含加工)三个生产过程,其精髓在于"草-畜结合""草-畜协调""草-畜互为依存、不可分割".草牧业试图解决长期困扰我国饲草料生产和畜牧业发展中的草畜矛盾、草畜"两张皮"的问题,强调饲草料(广义的牧草、饲草,包括饲用作物和饲用木本植物)在畜牧业发展中的基础和决定作用;与传统畜牧业相比,它具有明显的科学性、生态性和先进性.本文在简述草牧业内涵及其意义的基础上,论述了我国草牧业发展中存在的关键科技问题及其解决的途径;提出了发展草牧业应遵循的8个基本原理,即草畜良种选育原理、人工草地建植原理、天然草地保护恢复与适度利用原理、草地的生产功能和生态功能合理配置原理、饲草高效收获与加工原理、畜禽高效饲喂原理、水分有效利用原理以及区域系统发展原理,它们构成了草牧业发展的科学基础.为了践行"草牧业"理念,在内蒙古呼伦贝尔农垦集团开展了草牧业试验示范工作,取得了显著的生态和经济效益