食管癌患者血清可溶性肿瘤坏死因子受体水平测定

背景:目前尚无敏感性和特异性较高的血清肿瘤标志物用于临床诊断食管癌。目的:通过测定食管癌患者血清可溶性肿瘤坏死因子受体(sTNFR)水平的变化,探讨其在食管癌诊断和监控中的临床意义。方法:应用双抗体夹心酶联免疫吸附测定(ABC鄄ELISA)检测56例食管癌患者和10名健康成人的血清sTNFR鄄Ⅰ、sTNFR鄄Ⅱ水平,并与血清癌胚抗原(CEA)水平进行比较。结果:食管癌患者的血清sTNFR鄄Ⅰ和CEA水平显著高于健康对照组(P<0.001),而sTNFR鄄Ⅱ水平则显著低于健康对照组(P<0.001);sTNFR鄄Ⅰ与CEA的阳性率无显著差异,但均显著低于sTNFR鄄Ⅱ(P<0.001)。食管癌切除术后,患者的血清sTNFR鄄Ⅰ、sTNFR鄄Ⅱ水平均呈升高趋势,而CEA水平则无明显变化。结论:同时检测血清sTNFR鄄Ⅰ、sTNFR鄄Ⅱ水平有助于诊断食管癌和评估机体的免疫状态

大功率开关电源叠层母排方法

本发明涉及一种大功率开关电源B型叠层母排方法，即在大功率开关电源设计中，不用导线连接，而采用铜板连接，用铜板取代导线，铜板与铜板之间铜板之间都是相叠而安装的，完成了滤波电容，功率开关管或整流器多个器件的电气连接。本发明减小了开关柜的体积，又减少了内部连线，达到了抑制电磁干扰的目的

Preparation of Deacetylmycoepoxydiene with Reversed Phase Chromatography

研究不同条件下反相柱层析对去乙酰真菌环氧乙酯分离纯化的影响.通过rP-18中压柱层析对从红树林内生真菌拟茎点霉(PHOMOPSISSP.)A-1-2-3诱变后突变株g1-1固体发酵提取物中的去乙酰真菌环氧乙酯进行了纯化精制.对rP-18柱层析纯化去乙酰真菌环氧乙酯操作条件进行了考察,使去乙酰真菌环氧乙酯的含量从28%左右提高到81.5%左右,回收率大于90%.采用rP-18直接洗脱,溶析结晶,重结晶,结晶残液rP-18分步洗脱的总工艺路线,去乙酰真菌环氧乙酯的总回收率为87.7%,产品最终纯度达到99%以上.The effects on purification of deacetylmycoepoxydiene(DAM) in different conditions were studied.Preparation of DAM from the crude extract of fermentation of strain G1-1,a mutant strain of mangrove endophytic phomopsis sp.A-1-2-3,was conducted using C18-silica gel reversed phase chromatography at moderate pressure.In the process,the operating conditions of C18-silica gel chromatography were studied experimentally,then the purity of DAM reached about 81.5% from about 28% in the crude extract,and the recovery of DAM was over 90%.The whole process of preparation was as follow: direct elution with RP-18,solvent-out crystallization,recrystallization and segment elution of residue of crystallization with RP-18.Through the whole process flow,the general recovery of DAM was 87.7%,and the purity of DAM reached 99% or higher.国家海洋863项目(2007AA091503)资

Feeding habit of Erisphex potti from Changjiang Estuary and adjacent sea in spring

采用碳氮稳定同位素技术和聚类分析法对春季长江口及其邻近海域虻(Erisphex potti)的饵料组成、摄食随体长的变化进行研究,结果表明:(1)虻的食物由大于900μm的浮游动物、细螯虾(Leptochela gracilis)、太平洋褶柔鱼(Todarodes pacificus)、双喙耳乌贼(Sepiola birostrata)、火枪乌贼(Loligo beka)、日本枪乌贼(Loligojaponica)、脊腹褐虾(Crangon affinis)和仔稚鱼组成,优势饵料为浮游动物,其重量贡献率为69%~75%。(2)虻的食物组成及摄食量在体长为60mm时发生较大变化,脊腹褐虾、仔稚鱼及软体动物等饵料对体长大于60 mm的虻的贡献率增大,增幅为1%~12%。In order to assess the relative trophic importance of the spotted velvetfish in Changjiang Estuary and adjacent sea in summer,carbon(δ~(13)C) and nitrogen(δ~(15)N) stable isotope ratios were determined in spotted velvetfish and its potential food sources.Based on an organism's stable isotope ratios are an integration of the isotopic signatures of food sources that have been assimilated through time.The δ~(13)C and δ~(15)N values showed that the spotted velvetfish's food sources were composed of plankton >900 μm,Leptochela gracilis,common squid,Lantern cuttlefish,Beka squid, Common Japanese squid,larvae and juvenile and Japanese sand shrimp.plankton >900 μm was the most important food source,accounting for 69%-75% of the total food by weight.Food proportion of the spotted velvetfish varied among different classes of sizes.Cluster analysis dived the body lengths of the spotted velvetfish into two groups according to stable isotope ratios,>60 mm and 900 μm was 68%-87% to 75%-88% for the > 60 mm to 900 μm was the most important prey,while others were eaten occasionally by the spotted velvetfish.If the production of plankton >900 μm decrease in some cases,the spotted velvetfish would increase the other food proportion,sharing the large size food with other organisms,which would decrease the production of high trophic level organisms or shorten the length of food chain.国家自然科学基金重大项目(30490233);; 国家自然科学基金面上项目(30570293

一种用于促进红细胞生成的组合物及其用途

本发明提供了一种用于促进红细胞生成的组合物及其用途。本发明的组合物含有两种以上的作为活性成分的类黄酮化合物；其中所述的类黄酮化合物由下式I所示：式I，其中，R1为羟基或R2为H或羟基；并且在制备所述的组合物时，所述的类黄酮化合物是以纯化的形式使用的。本发明的组合物对增强HRE的转录活性、从而促进红细胞生成、以及预防和治疗贫血和/或缺氧症具有良好的协同效果，显著地降低了各组分的用量，价格低廉且毒副作用小