阵列式多通道芯片液相色谱初探

分离技术的微型化不仅可以节约试剂和样品,还具有高效、快速、高分辨和高通量等优点。本研究以微流控芯片作为液相分离平台,构建了阵列式并行多通道分离与多道检测系统。基于玻璃芯片、聚甲基丙烯酸甲酯芯片和聚二甲基硅氧烷芯片构建了三重复合芯片作为分离平台,实现了单张芯片上8个色谱填充床的制备。芯片系统的承压考察实验证明,玻璃芯片可以作为可靠的芯片材料支持压力驱动的芯片液相色谱。通过层级式分流获得单根芯片柱300 nL/min的纳升级流速。考察了8通道芯片色谱分离的可行性和有效性,获得了80000 plates/m的优良柱效和保留时间RSD=1.1%的柱间重现性。以蛋白酶解物为样品,考察了此系统在复杂生物样品高通量分离分析中的应用潜能。国家自然科学基金项目(Nos.21475110,21535007,J1310024);;中央高校基本科研业务费(No.20720160051);;厦门市科技计划项目(No.3502Z20173019)资助~

Survey on the Core Techniques of Cloud Computing

云计算作为一种新型的计算模式,在工业界和学术界得到了越来越广泛的应用和研究.本文首先阐述了云计算的基本概念,然后从云存储结构、编程框架、虚拟化和资源管理、安全与隐私、网络拓扑结构、能源管理等几个方面论述了当前云计算核心关键技术的发展现状,分析了其中的优势和不足,并进一步介绍了当前工业界主流的云计算平台以及学术界的开源云计算框架和实验系统.最后本文探讨了云计算进一步发展的研究问题,并展望了云计算的发展趋势.Cloud Computing as a new computing model, has been more widely used and studied in industry and academia. This paper introduces the basic concept of the Cloud Computing, and then discusses the c state-of-the-art of key technologies and analysises its trengths and weakness from the cloud storage architecture, programming framework, virtualization and resource management, security and privacy, network topology, energy management and other aspects of cloud computing. And further information on the current mainstream of the industrial cloud system and academic open source framework and test platform of Cloud Computing. Finally, this paper discusses the main challenges of cloud computing research issues,and the cloud computing trend

Benefit-Aware On-Demand Provisioning Approach for Virtual Resources

基于虚拟机的资源重配置操作具有耗时长并伴随性能衰减的特点。已有的 Cost-Oblivious 和 Cost-Aware方法在面对Internet突变负载时存在因经常执行资源重配置操作而导致频繁QoS违约的不足。提出了一种收益敏感(benefi t-aware)的资源按需提供方法。该方法首先考虑了 Web 应用在配置稳定期内的 QoS 获益,并以此构建收益函数;然后,以收益率最大化原则作为资源重配置策略选择的依据。实验结果表明,该方法与Cost-Oblivious方法相比最多能 节约25%的资源成本,而与Cost-Aware方法相比则能有效减少Web应用在突变负载下的QoS违约次数。It is a challenging problem to provide QoS (quality of service) in the virtualization-based cloud computing environment. Existing efforts have addressed this challenge based on either Cost-Oblivious approaches, or Cost-Aware approaches. However, both approaches may suffer frequent QoS violations under typical flash crowd workload. For instance, both approaches ignore the benefit gained after configuration changes. In this paper, a benefit-aware approach according to the profit rate maximization principle is introduced to address this problem. Here, the benefit means the satisfaction percept of the duration that application continuously guarantees the QoS in the new configuration. Experimental results based on TPC-W benchmark show that this benefit-aware approach can save the costs of VM resources as much as 25%and can effectively reduce the QoS violations compared through a cost-aware approach

Efficient Management System for Virtual Machine Snapshots

链式虚拟磁盘快照技术是广泛实现的一种支持虚拟机失效恢复的技术手段，针对链式结构磁盘快照技术引入多余I/O请求导致虚拟机性能低效问题，分析和研究了支持高效虚拟机快照实现的无链式关联磁盘快照技术，通过集成ZFS及OCFS2文件系统给出了无链式关联磁盘快照技术实现，并设计和实现了虚拟机快照存储组织模型和快速检索算法，提高了虚拟机快照检索效率。设计试验验证了系统的有效性。 Virtual disk snapshot is a prevailing technology to support virtual machine failover. It’s usually organized into linked structure, which may cause virtual machine performance degradation because this method would introduce additional disk I/O overhead. In this paper, we analyze the efficiency of disk snapshot technology with non-linked structure. By integrating ZFS and OCFS2 file system based on non-linked structure, we implement an efficient management system for virtual machine snapshots. In order to improve the efficiency of snapshot retrieval, we also propose a virtual machine snapshot storage organization model and optimize a snapshot search algorithm. The result of comparative experiment indicates our system’s effectiveness

A ROLE-BASED METHOD FOR SOLVING SINGLE POINT OF FAILURE IN VIRTUAL MACHINE RESOURCE POOL

现有的虚拟机池化管理普遍采用的是master/slave模式，存在单点失效问题。针对此问题，设计并实现一种基于角色的虚拟机池单点失效处理方法。该方法采用角色划分和选取机制自动指定主备节点，并基于序列法解决了自动选取机制中存在多个master节点冲突问题。实验表明，该方法能够实现master节点对用户透明的失效恢复。 Themaster/slavemodeisthemostpervasivemanagementpolicyforvirtualmachineresourcepoolatpresent,buthasthe problem of the single point of failure.In light of this,we design and implement a role-based method to solve the single point of failure for virtual machine resource pool.In this method,the master or backup node can be automatically activated according to the role appointment and the selection mechanism.Furthermore,a sequence method is proposed to solve the conflicts among multiple master nodes in automatic selection mechanism.Experiments show that the method can realise the recovery against the failure of transparency of master node on users.The master/slave mode is the most pervasive management policy for virtual machine resource pool at present, but has the problem of the single point of failure. In light of this,we design and implement a role-based method to solve the single point of failure for virtual machine resource pool. In this method, the master or backup node can be automatically activated according to the role appointment and the selection mechanism. Furthermore, a sequence method is proposed to solve the conflicts among multiple master nodes in automatic selection mechanism. Experiments show that the method can realise the recovery against the failure of transparency of master node on users

利用本体的WFS要素语义检索研究

为了实现基于多源WFS服务要素层次的语义检索,提出了一个要素本体的五元模型。在此框架下,研究了要素本体的自动构建方法,形式化定义了要素的空间关系,并扩展了逻辑关系以及空间关系的推理规则来实现要素层次的联合推理检索,并给出了联合规则推理的要素语义检索算法。实例证明,本文方法对多源WFS的语义查询具有实际的可行性,为多源WFS服务要素的语义检索提供了一个新的方法

焦化废水强化处理关键技术研究与探讨

介绍了通过真空碳酸钾脱硫废液预处理工艺的开发和专用药剂的应用,可分别去除废液中99%以上的硫化物和90%以上的氰化物;研究了A2/O2强化生物脱氮技术,COD和氨氮去除率均可在95%以上;经开发的专用高效脱氰混凝剂,COD去除率达40%以上,总氰化物降至0.2mg/L以下。研制了高效非均相臭氧催化剂,结合开发的两段法催化氧化设备可使COD去除率在50%以上,臭氧利用率达80%以上,示范工程出水各项指标均可达到《炼焦化学工业污染物排放标准》(GB16171—2012)要求

焦化废水强化处理关键技术研究与探讨

介绍了通过真空碳酸钾脱硫废液预处理工艺的开发和专用药剂的应用,可分别去除废液中99%以上的硫化物和90%以上的氰化物;研究了A2/O2强化生物脱氮技术,COD和氨氮去除率均可在95%以上;经开发的专用高效脱氰混凝剂,COD去除率达40%以上,总氰化物降至0.2mg/L以下。研制了高效非均相臭氧催化剂,结合开发的两段法催化氧化设备可使COD去除率在50%以上,臭氧利用率达80%以上,示范工程出水各项指标均可达到《炼焦化学工业污染物排放标准》(GB16171—2012)要求

Effects of NS-Lactobacillus Complex on Immunity Levels in Weaning Piglets

选择二元杂交(杜/长)20日龄断奶仔猪90头,平均断奶体重8.99kg±1.60kg,按体重和性别分成3个处理组,每处理3个重复。A组为对照组,饲喂玉米、豆粕基础日粮;B组为基础日粮添加NS复合乳酸菌组2g/kg;C组为基础日粮添加抗生素组(2g/kg金霉素、0.25g/kg阿莫西林和0.125g/kg的支原净)。试验共30d,研究微生态制剂NS复合乳酸菌对断奶仔猪体液免疫和细胞免疫水平的影响。结果表明,试验30d时,NS复合乳酸菌组与对照组相比,断奶仔猪血清中的IgM含量和干扰素INF-γ的含量分别高出19.7%和10.2%,猪瘟病毒抗体阳性率高出33.3%,差异显著(P<0.05)。NS复合乳酸菌制剂对加强仔猪体液免疫,维持仔猪的猪瘟、伪狂犬病毒抗体水平和促进机体细胞免疫具有良好效果

The Effects of NS-lactobacillus Complex on Productivity Performance, Plasma Biochemical Indexes and Dilarrhea Rate in Weaned Piglets

选择二元杂交(杜/长)的(28)日龄断奶仔猪90头,平均断奶体重(8.99&plusmn;1.60)kg,按体重和性别分成3个处理组,每处理3个重复。A组为对照组,饲喂玉米、豆粕基础日粮;B组为添加0.2%(m/m)NS复合乳酸菌制剂到基础日粮中;C组为添加0.2%(m/m)金霉素、0.025%(m/m)阿莫西林和0.012 5%(m/m)的支原净。试验分为3个阶段:试验共30 d,研究微生态制剂NS复合乳酸菌对断奶仔猪生产性能、血液生化指标和减少腹泻率的影响。结果表明,NS复合乳酸菌能显著提高断奶仔猪的生产性能,平均末重、平均增重、平均日增重分别比A组高9.12%,7.64%和7.67%,差异显著(P〈0.05);料肉比降低9.70%,差异显著(P〈0.05);有效降低断奶仔猪的腹泻率50%,差异极显著(P〈0.01);B组的血糖(GLU)含量比A组高20%、超氧化物歧化酶(SOD)活性高10.9%,差异显著(P〈0.05),对增强机体抗氧化功能,减少应激作用具有良好效果。</p