Study on Corrosion Resistance of Zr-0.8Sn-1Nb-0.3Fe Alloy after Kr~+ Ion Irradiation

采用高压釜腐蚀实验研究了2种不同制备工艺下的Zr-0.8Sn-1Nb-0.3Fe合金(1#,2#)经360℃、5~25; dpa的Kr~+辐照后、在400℃/10.3 MPa过热蒸汽中的耐腐蚀性能,用透射电子显微镜(TEM)、扫描电镜(SEM)、; X射线衍射仪(XRD)分析合金腐蚀后氧化膜显微组织结构。结果表明,100; d腐蚀后,合金的腐蚀增重随着辐照剂量的增加而增加,由于1#合金中的第二相比2#合金更为细小、弥散,相同辐照剂量下,前者的腐蚀增重较低。腐蚀转折前; ,从蒸汽腐蚀侧到锆合金基体,氧化膜中的氧含量逐渐降低,靠近蒸汽侧的氧化膜主要由等轴晶形态的单斜ZrO_2组成,而基体界面处的氧化膜主要为柱状晶形; 态的四方ZrO_2和六方Zr_3O;腐蚀转折后,基体界面处的氧化膜呈"花菜"状生长,"花菜"尺寸大小与氧化膜生长速率的高低及不均匀生长趋势的大小; 呈对应关系。The corrosion resistance of Zr-0.8Sn-1Nb-0.3Fe alloys prepared by two; different processes was investigated in 400℃/18.6MPa superheated steam; by static autoclave after irradiated by 360℃ with Kr~+-irradiation of; 5~25 dpa. The microstructures of oxidation film after corrosion were; analyzed by TEM, SEM, and XRD. The results showed that the corrosion; weight-gain increased with the irradiation dose, while the weight-gain; of 1# alloy with smaller and more dispersive SPPs than 2# alloy was; lower under the same irradiation dose. Before corrosion turning, the; oxygen content in the oxidation film decreased from the steam-side to; the zirconium matrix. The oxidation film beside the steam-side was; mainly composed by equiaxied monoclinic ZrO_2 crystal, while near the; film/matrix interface by columnar quartet ZrO_2 crystal and hexagonal; Zr_3O crystal. After transition of corrosion weight, the film near the; interface grew like cauliflowers, and the size of cauliflowers were; corresponded to the growth rate and uneven growth trend of oxidation; film

Expression of RGS16 protein induced by wide type p53 gene in rat glioma C6 cell line

目的:探讨内外源性野生型p53是否可以诱导大鼠胶质瘤细胞C6RGS16表达。方法:在大鼠胶质瘤细胞C6中,分别转染pEGFP-C3-wtp53或以400ng/ml表阿霉素诱导内源性野生型p53表达,于处理后0h、4h、8h、16h、26h、32h和52h收集细胞爬片、固定后免疫细胞化学检测p53蛋白与RGS16蛋白表达。结果:转染pEGFP-C3-wtp53后4h、8h、16h、26h和32h时均检测到p53蛋白表达,在8h和16h时检测到RGS16蛋白表达;在400ng/ml表阿霉素处理后,仅在26h时检测到p53表达而始终未见到RGS16蛋白表达。结论:内外源性野生型p53可以诱导大鼠胶质瘤细胞C6RGS16蛋白的表达

Effect of Exogenous RGS16 Gene Transfection on Growth of Glioma Cell Line C6

背景与目的:有研究表明野生型p53可以诱导RGS16表达,而RGS16可能与胶质瘤的发生有关。本研究旨在探讨RGS16基因转染对大鼠胶质瘤C6细胞生长的影响。方法:构建真核表达载体pIRES2-EGFP-RGS16,脂质体法转染C6细胞,经G418筛选后荧光显微镜观察细胞中增强型绿色荧光蛋白(enhancedgreenfluorscentprotein,EGFP)的表达,RT-PCR证实RGS16的mRNA表达,免疫细胞化学方法检测细胞中RGS16蛋白表达。最后利用流式细胞仪、生长曲线、平板克隆形成等方法研究RGS16基因对胶质瘤细胞周期、生长及增殖的影响。结果:成功构建了稳定表达RGS16和表达空载体的细胞系C6-RGS16、C6-GFP。C6-RGS16、C6-GFP和C6经流式细胞仪检测S期的细胞比例分别为28.5%、18.9%和14.3%(P<0.05);生长曲线表明C6-RGS16生长的速度明显快于C6-GFP及C6,但其平板克隆形成率分别为12%、25%和25%(P<0.05)。结论:RGS16促进C6细胞周期从G1期向S期过渡,加快细胞生长速度,但是并不促进细胞克隆形成。 【英文摘要】 BACKGROUND & OBJECTIVE: Wild type p53 could induceRGS16 mRNA expression, and RGS16 protein is related with carcinogenesisof glioma. This study was designed to investigate the effects of exogenousRGS16 gene transfection on the growth of rat glioma cell line C6.METHODS : A eukaryotic expression plasmid pIRES2-EGFP-RGS16 wasconstructed, and transfected into C6 cells. The cells were selected by G418.The expression of enhanced green fluorescent protein (EGFP) was observedunder fluorescent microscope, and the exp...军队医药卫生科研基金项目(No.02ma04)~

Regulation of Cell Cycle of Glioma C6 Cells by Regulator of G Protein Signaling 16

目的:探讨G蛋白调节子 16(RGS16)对胶质瘤C6细胞周期的影响。方法:利用脂质体介导法将RGS16基因导入C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁生长情况 ;免疫细胞化学法检测转染前后RGS16蛋白的表达情况 ;流式细胞仪检测转染pCMV 5 RGS16和 pCMV 5质粒后每隔 12h后的细胞周期变化。 结果:转染pCMV 5 RGS16质粒 2 4h后 3 0 .0 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ,72h之后细胞又恢复正常 ;RGS16蛋白的表达呈时相性 ,3 6h时表达率最高 (阳性率为13 .0 % ) ,72h表达终止 ;C6细胞的各期细胞比例变化与RGS16蛋白表达对应 ,在 3 6h时G1期比例从转染前的 70 .5 %降低到60 .2 % ,S期比例从 2 0 .9%增加到 3 4.9% ;在 48h时G1期增加到 76.2 % ,S期减少到 11.4% ;72h各期恢复到正常比例。对照组细胞转染前后形态变化不明显 ,RGS16蛋白表达阴性 ,细胞周期变化不明显。结论:RGS16能促进C6细胞周期的运行. 【英文摘要】 Objective To study the effect of regulator of G protein signaling 16(RGS16) on the cell cycle of glioma C6 cell.Methods pCMV5 RGS16 was transfected into C6 cells by lipofectin.The morphological and adhesive changes of the cells were observed under an inverted microscope.Expression of RGS16 was examined by immunocytochemical method both before and after the transfection.Flow Cytometry was adopted to measure the fraction number changes of the cell cycle phase every 12 h.Results 24 hours after the transfec..

Construction of the eukaryotic expression vector pIRES2-EGFP-Axin and its expression in glioma cells

目的:构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞系C6中进行表达。方法:用PCR的方法扩增Axin基因,构建真核表达载体pIRES2EGFPAxin,经NheI及SalI双酶切鉴定并测序。通过脂质体法转染C6细胞,用荧光显微镜检测细胞中增强型绿色荧光蛋白(EGFP)的表达,用免疫组化染色的方法检测细胞中Axin的表达。结果:构建了真核表达载体pIRES2EGFPAxin,用脂质体法转染神经胶质瘤细胞C6后,经荧光显微镜和免疫组化染色法检测,可见细胞内有EGFP及Axin的表达。结论:成功地构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞C6中表达,为研究Axin对肿瘤的生物学作用以及Axin在肿瘤基因治疗中的应用奠定了基础。国家杰出青年自然科学基金项目(No.30125012);; 军队医药卫生科研基金项目(No.02ma04);; 高等学校骨干教师及归国留学人员科研启动基金(No.1999747

Construction of the eukaryotic expression vector pIRES2-EGFP-Axin and its expression in glioma cells

目的:构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞系C6中进行表达。方法:用PCR的方法扩增Axin基因,构建真核表达载体pIRES2EGFPAxin,经NheI及SalI双酶切鉴定并测序。通过脂质体法转染C6细胞,用荧光显微镜检测细胞中增强型绿色荧光蛋白(EGFP)的表达,用免疫组化染色的方法检测细胞中Axin的表达。结果:构建了真核表达载体pIRES2EGFPAxin,用脂质体法转染神经胶质瘤细胞C6后,经荧光显微镜和免疫组化染色法检测,可见细胞内有EGFP及Axin的表达。结论:成功地构建真核表达载体pIRES2EGFPAxin,并在神经胶质瘤细胞C6中表达,为研究Axin对肿瘤的生物学作用以及Axin在肿瘤基因治疗中的应用奠定了基础。 【英文摘要】 AIM: To construct the eukaryotic expression vector pIRES2-EGFP-Axin, and to express Axin in C6 glioma cells. METHODS: The Axin gene was amplified by PCR using pCMV5-HA-Axin as a template, and confirmed by DNA sequencing. The eukaryotic expression vector pIRES2-EGFP-Axin was constructed by introducing Axin DNA fragment into the sites of Nhe I and Sal I of pIRES2-EGFP vector. The plasmid was transfected into the C6 cells using lipofectamine. The expressed EGFP was observed under fluorescent microscope and the...国家杰出青年自然科学基金项目(No.30125012);; 军队医药卫生科研基金项目(No.02ma04);; 高等学校骨干教师及归国留学人员科研启动基金(No.1999747

Effect of Exogenous RGS16 Gene Transfection on Growth of Glioma Cell Line C6

背景与目的:有研究表明野生型p53可以诱导RGS16表达,而RGS16可能与胶质瘤的发生有关。本研究旨在探讨RGS16基因转染对大鼠胶质瘤C6细胞生长的影响。方法:构建真核表达载体pIRES2-EGFP-RGS16,脂质体法转染C6细胞,经G418筛选后荧光显微镜观察细胞中增强型绿色荧光蛋白(enhancedgreenfluorscentprotein,EGFP)的表达,RT-PCR证实RGS16的mRNA表达,免疫细胞化学方法检测细胞中RGS16蛋白表达。最后利用流式细胞仪、生长曲线、平板克隆形成等方法研究RGS16基因对胶质瘤细胞周期、生长及增殖的影响。结果:成功构建了稳定表达RGS16和表达空载体的细胞系C6-RGS16、C6-GFP。C6-RGS16、C6-GFP和C6经流式细胞仪检测S期的细胞比例分别为28.5%、18.9%和14.3%(P<0.05);生长曲线表明C6-RGS16生长的速度明显快于C6-GFP及C6,但其平板克隆形成率分别为12%、25%和25%(P<0.05)。结论:RGS16促进C6细胞周期从G1期向S期过渡,加快细胞生长速度,但是并不促进细胞克隆形成。军队医药卫生科研基金项目(No.02ma04)~

Effect of Exogenous RGS16 Gene Transfection on Growth of Glioma Cell Line C6

背景与目的:有研究表明野生型p53可以诱导RGS16表达,而RGS16可能与胶质瘤的发生有关。本研究旨在探讨RGS16基因转染对大鼠胶质瘤C6细胞生长的影响。方法:构建真核表达载体pIRES2-EGFP-RGS16,脂质体法转染C6细胞,经G418筛选后荧光显微镜观察细胞中增强型绿色荧光蛋白(enhancedgreenfluorscentprotein,EGFP)的表达,RT-PCR证实RGS16的mRNA表达,免疫细胞化学方法检测细胞中RGS16蛋白表达。最后利用流式细胞仪、生长曲线、平板克隆形成等方法研究RGS16基因对胶质瘤细胞周期、生长及增殖的影响。结果:成功构建了稳定表达RGS16和表达空载体的细胞系C6-RGS16、C6-GFP。C6-RGS16、C6-GFP和C6经流式细胞仪检测S期的细胞比例分别为28.5%、18.9%和14.3%(P<0.05);生长曲线表明C6-RGS16生长的速度明显快于C6-GFP及C6,但其平板克隆形成率分别为12%、25%和25%(P<0.05)。结论:RGS16促进C6细胞周期从G1期向S期过渡,加快细胞生长速度,但是并不促进细胞克隆形成。 【英文摘要】 BACKGROUND & OBJECTIVE: Wild type p53 could induceRGS16 mRNA expression, and RGS16 protein is related with carcinogenesisof glioma. This study was designed to investigate the effects of exogenousRGS16 gene transfection on the growth of rat glioma cell line C6.METHODS : A eukaryotic expression plasmid pIRES2-EGFP-RGS16 wasconstructed, and transfected into C6 cells. The cells were selected by G418.The expression of enhanced green fluorescent protein (EGFP) was observedunder fluorescent microscope, and the exp...军队医药卫生科研基金项目(No.02ma04)~

Expression and relationship of RGS16 and p53 in human glioma

目的研究G蛋白信号调节子16(RGS16)与p53在人胶质瘤中表达及其相关性。方法利用免疫组织化学链霉亲合素生物素过氧化物酶复合物(SABC)法检测42例胶质瘤标本中RGS16与p53的表达。结果RGS16在10例胶质瘤旁正常脑组织有8例神经元阳性但胶质细胞阴性、42例胶质瘤中37例肿瘤细胞阳性,二者差异显著(P0.05);其中,RGS16与p53共同阳性表达11例,共同阴性表达1例,Kappa检验二者表达呈负相一致(P<0.05)。结论RGS16在胶质瘤中高表达而与病理分级无关,推测RGS16可能在胶质瘤发生中起作用。另外,胶质瘤中RGS16与p53表达呈负相关。 【英文摘要】 Objective To study the expression of RGS16 in human glioma and its relationship with p53. Methods The expression of RGS16 and p53 in protein level was studied by immunohistochemistry strept avidin-biotion-peroxidase-complex (SABC) method in 42 samples.Results In 10 normal brain tissues beside the human glioma, 8 cases expressed RGS16 in the neurons,but none in gliocytes; in 42 human gliomas, 37 cases expressed RGS16 in the glioma cells, and the difference between the normal tissue and the glioma was observe...军队医药卫生科研基金资助项目(02ma04);; 国家杰出青年自然科学基金资助项目(30125012);; 高等学校骨干教师及归国留学人员科研启动基金资助项目([1999]747