Effect of Rab39 on Autophagy

Rab蛋白属于Ras超家族，是其中最大的一个家族。在哺乳动物细胞中已经发现70种以上的Rab蛋白。Rab蛋白通过结合GTP或GDP的转换来发挥其分子开关的作用，进而调控细胞囊泡的形成、靶向、黏附和融合，在细胞内蛋白质运输过程中扮演非常重要的角色，Rab功能异常与很多疾病的发生密切相关。Rab39是一种新发现的Rab蛋白，Rab39有两个成员：Rab39a和Rab39b。人类Rab39a基因定位于常染色体11q22.3上，而Rab39b基因定位于性染色体Xq28上。不管是对于Rab39a亦或是Rab39b，关于它们的研究都甚少。 本文通过对Rab39的定位研究发现，Rab39a和Rab39b都...Rab protein belongs to the Ras superfamily and is one of the largest family. More than 70 Rab proteins have been found in mammalian cells. Rab protein plays a very important role in the process of intracellular protein transport by regulating the formation, targeting, adhesion and fusion of cell vesicles by combining the effects of GTP or GDP conversion, The disease is closely related. Rab39 is a ...学位：理学硕士院系专业：药学院_化学生物学学号：3232014115340

县级财政科技投入结构及其动力研究——基于安徽省C县的实证调查

县域财政科技投入主要体现为财政局拨付、科技局统筹管理的分配模式,但农林等其他县直单位同样也存在科技投入。县本级科技支出居主导地位,其他资金主要来自于上级部门的科技专项。科技推广体系呈现以县级科技集群为轴心的向下辐射形态,与企业、各类生产合作社、个人发生复杂的供需互动,相应对各类需求主体也产生不同的效益。以科技考核竞争"、发展—晋升"竞争、建楼面子之争和邻区比对等构成的混合竞争系统,是影响县域财政科技投入的主要动力

Research on facial spectrum and color characteristics of five-zang disease status

目的:观察疾病状态人群面色光谱色度情况,为疾病状态五脏病评价提供可供依据。方法:采用日产柯尼卡美能达CM-2600d分光测色仪,观测健康组(183例)、疾病组(370例)额部、眉间部、鼻部、下颏、左右颧部、左右眼胞8处明亮度l、红光度A、黄光度b、饱和度C值及波长段(400~700nM)下的面色反射率值等指标,并据中医理论对疾病状态进行五脏病位分析。结果:疾病组l值显著低于健康组,b、C值均显著高于健康组;疾病组各波长段下反射率显著低于健康状态组(P<0.05)。疾病组除左右眼胞外的6个位点间的l、A、b、C值有显著性差异(P<0.05),不同点位有其特异性的色度变化特征。疾病组五脏病各组光谱色度特征比较有显著差异(P<0.05),结果与中医面部脏腑五色理论在一定程度上相符合。结论:运用光谱色度测定方法,可以作为诊断疾病状态五脏病的重要指标。Objective: To observe disease status facial spectrum and color,providing good quantitative basis for disease assessment.Methods: We gathered the facial color information in health(183) and disease groups(370) including L,a,b,C values and reflection of different wavelengths in 400-700nm with CM-2600D spectral photometric color measuring instrument on 8 points including frontal part,glabellas nose,mandible two cheeks and eye cells and analysized disease status five-zang positioning according to the principle of TCM syndrome differentiation.Results: L value of Disease group was lower than health status group and b,C values were higher than health status group,reflection of different wavelengths of disease group were lower than health group(P<0.05).6 points in disease group between loci L,a,b,C values with varying degrees of significant differences(P<0.05).Different point had its own specificity facial complexion variation characteristics.Facial spectrum and color of each five-zang disease group had significant difference through comparion of facial complexion.To a certain extent the result was consistent with TCM facial organs Five-color theory.Conclusion: There existed diagnostic value in distinguishing five-zang disease status in some degree by spectral photometric color measuring technique.国家科技支撑计划(No.2012BAI37B06);国家高技术研究发展计划(863计划)资助项目(No.2008AA02Z407);国家自然科学基金资助项目(No.30873463;No.81173200);国家自然基金青年项目(No.81102558);上海市重点学科(第三期)建设项目(No.S30302;No.S30303)---

Synonymous Codon Usage of Both Alternativelyand Commonly Spliced Genes in Human Chromosome 1 I: Synonymous Codon Usage Bias Analysis

人类1号染色体可变剪接(选择性剪接)基因344非冗余蛋白质编码序列(188183密码子)和普通剪接(非可变剪接)基因的386蛋白质编码序列(223116密码子)被用于研究人类密码子使用偏爱模式。全部密码子使用数据分析表明,人类可变剪接基因密码子的偏爱水平显著高于普通剪接基因。在人类1号染色体基因中,密码子第三位置的G+C含量有很大的异质性(0 24～0 95),并且可变剪接基因密码子第三位置平均G+C含量(64 66%)大于普通剪接基因(59 97%)。Nc值对GC3s图显示密码子偏爱使用除了受核苷酸组成制约外,其它的因子可能也影响密码子的使用变化。此外,可变剪接基因中以G或C结尾的密码子比普通剪接基因出现的频率高。密码子使用的差异可能是由可变剪接基因pre mRNA特有的结构特征和多种剪接模式决定的。It is already clear that alternative splicing has an extremely important role in expanding the protein diversity. Comparative study of the codon usage patterns of alternatively and commonly spliced genes may thereby be necessary. In this paper, the patterns of codon usage bias of two kinds of human genes, alternatively spliced genes and commonly spliced genes, were formulated through analyzing 344 non-redundant protein coding sequences from alternatively spliced genes (188183 codons) and 386 from commonly spliced genes (223116 codons) in human chromosome (1. Overall) codon usage data analysis indicated that the alternatively spliced genes showed a stronger codon usage bias than commonly spliced genes. Very extensive heterogeneity of G+C content in silent third codon position (GC3s) was evident among these genes, and GC3s content of alternatively spliced genes was higher than that of commonly spliced genes. G- or C-ending codons were more abundant in alternatively spliced genes than commonly spliced genes in human chromosome 1. The causation of differences created could be explained by pre-mRNA structural characteristics of alternatively spliced genes influencing their codon usage bias.Chinapostdoctoralprogramsfoundation( 2 0 0 2 11

Synonymous Codon Usage of Both Alternativelyand Commonly Spliced Genes in Human Chromosome 1 II: Expression Pattern and Gene Length Shape Codon Usage

进一步研究基因表达水平和基因长度与密码子使用偏爱之间的关系。多变量统计分析发现,人类1号染色体选择性剪接基因和普通剪接基因密码子使用变化都呈现单一趋势,且它们之间的密码子使用模式也非常相似,推测的高表达基因确实偏爱以C或G结尾的密码子,基因表达水平与密码子使用偏爱之间的关联也达到显著水平。因此,人类1号染色体高表达基因密码子的使用偏爱可能主要被翻译选择所决定。此外,基因长度与密码子偏爱水平之间也存在高度相关,说明相对较短的基因具有较高的密码子使用偏爱,翻译选择可能缩短了高表达基因的长度从而提高翻译效率。We further explored the correlation between synonymous codon bias and gene expression level and gene length. Correspondence analysis showed that there was only a single trend in codon usage variation among alternatively and commonly spliced genes, and the pattern of codons usage was very similar in them. The putatively highly expressed genes preferred C- or G-ending codons, and the correlations between synonymous codon bias and gene expression levels were also very significant, which implied that translation selection might play an important role in dominating codon usage bias in highly expressed genes in humans. Surprisingly, a strong correlation between codon-usage bias and gene length was also observed, e.g. the shorter a gene′s length, the higher its expression capacity. It suggested that the selection of codons might result in the reduction of the length of highly expressed genes and enhancement of translational efficiency.Chinapostdoctoralprogramsfoundation( 2 0 0 2 11

Transcription of turbot Scophthalmus maximus Mx gene induced by polyinosinic-polycytidylic acid given in different ways

以POlyI∶C(又称聚肌胞)为诱导剂,分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆SCOPHTHAlMuS MAXIMuS体内抗病毒蛋白MX基因的转录,利用半定量rT-PCr方法检测干扰素下游基因-MX基因的转录水平来确定该诱导剂的诱导效果。结果显示,以上3种途径都能高效诱导MX蛋白MrnA的转录,均在48H之后达到高峰,其中以浸泡的方式更容易诱导MX基因转录,且在120H时仍保持较高水平。MX基因转录的时相变化证明了国产POlyI∶C可以通过多种途径诱导抗病毒蛋白MX的表达,为实际应用中确定用药途径提供了理论依据。另外,实验初步建立了半定量rT-PCr方法,为检测鱼体内干扰素的表达提供了技术方法。Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI∶C with intraperitoneal injection,immersion and oral administration.Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI∶C’s induction.The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI∶C application.The immersion method seemed to be the most effective way to induce Mx mRNA,as Mx mRNA could last more than 120h at a high level.This experiment proved that the homemade PolyI∶C could effectively induce Mx mRNA transcription in different applications,which provided a reference to use PolyI∶C in aquaculture practice.国家863计划(2003AA622070);国家十一五科技支撑计划(2006BAD09A11);行业专项(nyhyzx07-046)共同资

Primary Exploration on Quality Evaluation of Holothurian Sold on the Market by HPLC Fingerprint

以10批不同产地刺参HPlC指纹图谱中的6个共有峰为评价指标,结合相似度分析,对不同刺参质量进行评价和检验。结果表明:不同批次刺参样品的6个色谱峰在指纹图谱分析过程中色谱行为相同,峰面积大,特征性强,能反映刺参的固有化学特征。结合相似度分析,可用于刺参质量评价。An optimized high performance liquid chromatography (HPLC) method was developed for the analysis of holothurian and the HPLC fingerprint was established from 10 batches of the holothurians from different habitats.The HPLC fingerprint showing 6 common characteristic peaks was used to explore the quality evaluation of holothurian and distinguish from the fakes with the similarity analysis.This method is accurate and reliable,providing a scientific basis for the quality control of holothurian and can be used to evaluate the quality of holothurian sold on the market.我国近海海洋综合调查与评价908专项(908-02-05-04):海洋药用生物资源评价和《中华海洋本草》编纂;海洋一所基本科研业务专项(GY-022008T32):海洋绿藻活性成分分析、鉴

Damage to mangroves from extreme cold in early 2008 in southern China

通讯作者Author for correspondence (E-mail: [email protected])2008年初,我国南方19个省经历了50年一遇的持续低温雨雪冰冻天气。极端气候对华南沿海各省的红树林区造成不同程度的危害。2008年3月,在我国南方各省红树林区的10个代表性地点,对这次寒害造成的红树植物伤害程度进行了系统的调查。结果表明:冬季低温对红树林的影响极为显著,特别是在低纬度的海南、广西和广东湛江,由于极端低温正值夜间退潮,对红树林的影响更为显著;在纬度较高的福建,本地红树种类秋茄(Kandelia obovata)、桐花树(Aegiceras corniculatum)和白骨壤(Avicennia marina)及引种的木榄(Bruguiera gymnorrhiza)等,由于长期适应于冬季较低的气温或在种植前经过抗寒锻炼,具有较强的抗寒能力。各地主要红树植物中,广布种秋茄、桐花树和白骨壤最为耐寒,其耐寒性均大于红树科的木榄、海莲(Bruguiera sexangula)和红海榄(Rhizophora stylosa)。海桑(Sonneratia caseolaris)对温度的敏感性最强,抗寒能力最低,因此,即使在其原产地海南也受到较为严重的寒害,在纬度更高的引种地出现大面积受害甚至全部死亡,而从孟加拉国引种的无瓣海桑(Sonneratia apetala)却显示出一定的抗寒能力。同一地点的红树植物幼苗的抗寒能力低于大树。此次寒害也造成了苗圃场的种苗大量死亡,成熟的植株提前落花落果,这势必会影响后继一两年内红树林的自然更新和人工造林。因此,在未来红树林造林或人工引种中,一定要考虑到红树植物的抗寒能力。国家自然科学基金(30671646和30700092);厦门大学“闽江学者”启动基

表面活性剂辅助球磨——一种制备稀土永磁材料的新工艺

介绍了采用表面活性剂辅助球磨工艺制备永磁纳米材料,主要制备了永磁纳米颗粒、各向异性永磁纳米片、低温制备纳米材料以及"自下而上"技术制备纳米晶块状材料。该方法不仅适用于稀土永磁纳米材料的制备,也可用于软磁纳米片和颗粒的制备。表面活性剂辅助球磨制备的永磁纳米颗粒和片状材料在众多领域具有重要应用。采用X射线衍射仪、扫描电子显微镜以及振动样品磁强计等技术研究了不同的表面活性剂辅助球磨工艺对粉体微结构、形貌及磁性能的影响。研究表明,表面活性剂在球磨过程中发挥了重要的作用,改变表面活性剂的种类和工艺条件,不仅能调控粉体的形态,还能控制粉体的磁性能,但该工艺仍存在很多技术难题和理论问题需要进一步的研究