Proteomics approach for E.coli K-12 outer membrane proteins related to disinfectant tolerance and antibiotic resistance

采用蛋白质组学和基因组学研究方法，对革兰氏阴性病原菌大肠杆菌K-12的外膜蛋白进行了较系统的研究，重点筛选和鉴定了与消毒剂耐受和抗生素耐药相关的蛋白质组，并对这些差异外膜蛋白进行验证和一系列的功能研究，有助于了解细菌对消毒剂耐受和对抗生素耐药的机制，这对于寻找新药物靶点和研制新的抗菌药物或疫苗具有重要的意义。 通过对多种消毒剂的蛋白质组学进行分析，成功地在大肠杆菌K-12中分别鉴定出与异丙醇(7种)、乙醇(7种)和苯酚(5种)耐受相关的外膜蛋白，并发现异丙醇与乙醇耐受相关外膜蛋白一致，均为Tsx、OmpC、OmpF、FadL、OmpT、OmpA和LamB，其中FadL、OmpT、OmpA、L...Using proteomics and genomics technologies, we invesigated the outer membrane proteins of Gram-negative bacterium E. coli K-12 in response to disinfectant-tolerance and drug-resistance. This investigation was performed with the use of proteomic methodologies for altered proteome, Western blotting for verification of the altered proteins, and gene-deficient and protein-overexpressed approch for pr...学位：理学博士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：B20042602

The Research on Instauration and Basic System Design of Chinese Property Tax

随着住房制度的深入改革，我国房地产业得到了迅猛发展，已经日渐成为国民经济的“支柱产业”。但是在房地产业发展的同时，成型于五十年代的房地产税制却多年未变。2003年底党的十六届三中全会通过的《关于完善社会主义市场经济体制若干问题的决定》中提到：“实施城镇建设税费改革，条件具备时对不动产开征统一规范的物业税，相应取消有关收费。”自此，“物业税”一词引起了政府、学术界、实业界的高度关注，成为学术界研究和媒体报道的热点。不少专家、学者通过与国外房地产税制的对比分析，结合我国国情，提出了我国房地产税制改革的一些思路。但是目前的研究还存在很多不足，因此我国物业税的研究仍然任重道远。 本文在结合前人研究的...China's real estate industry has greatly developed along with the deeply reform of housing rules and regulations, and has become the support industry of national economy. However, China's real estate tax system has not changed ever since 1950’s, whose characters are not fit for the new requirements of economic development. In Oct, 2003, CCP passed 《CCP′s Resolutions On Perfecting Socialism Market ...学位：经济学硕士院系专业：经济学院财政系_财政学(含税收学)学号：1552006115094

Association of TolC expression with resistance to tetracycline in Escherichia coli K-12

随着抗生素的开发与使用,细菌在对多种抗生素的适应过程中逐渐发展出对抗生素耐药的反应机制。TolC是药物排出转运体系的外膜成分,与AcrAB一起形成主要的药物排出泵,有关其表达量与耐药间的关系目前尚不清楚。试验将tolC克隆到pET-32a载体上进行诱导表达,镍柱纯化,免疫新西兰大白兔,获得1:4000的抗血清。Western blotting分析表明,TolC的表达量在耐四环素的大肠埃希菌K-12中比对照组提高50%。细菌TolC高表达试验发现,其MIC从100μg/ml提高到200μg/ml。结果说明TolC的表达量与四环素耐药性直接相关,提示细菌可以通过调节外膜蛋白的表达实现对抗生素的耐受。Association of TolC expression with resistance to tetracycline in Escherichia coli K-12 was studid.TolC was an outer membrane protein,which was bound with AcrAB to form a critical multidrug efflux pump.However,association of TolC amount with drug resistance was ill-defined.In the study,tolC was cloned into pET-32a vector induced expression and its recombinant proteins were purified by Ni-NTA.The purified recombinant proteins were used for preparing of anti-TolC.The prepared antiserum with titer 1:4000 was utilized as the primary antibody in Western blotting.It showed that TolC amount was increased 50% in tetracycline-resistant E.coli K-12 compared to its original control strain.When TolC was overexpressed,the strain showed strong resistance to tetracycline with its MIC from 100μg/ml to 200μg/ml.These findings indicated that TolC amount was directly correlated to bacterial resistance to tetracycline,suggesting that bacteria mounted an adaptive feedback to antibiotics by regulating outer membrane protein expression

新型Bola两亲分子作为基因治疗药物输递载体的研究

为检测新型Bola两亲分子(Orn-C16-G)用作基因载体的性能,制备了Bola与siRNA的复合物(Bolaplexes),用原子力显微镜观察其在不同复合比下的形貌;并转染Hela细胞,用CCK-8检测Bolaplexes的细胞毒性,用荧光显微镜、流式细胞仪表征Bolaplexes的细胞内吞效果。实验结果显示,复合比1∶1条件下,Bola分子可以有效地压缩siRNA分子形成较小尺寸的复合物(100～200 nm)。Bolaplexes可高效地进入Hela细胞,并且毒性低于商售转染试剂,有较好的应用前景。本研究为下一步Bola两亲分子用于基因治疗的临床研究奠定了基础

彩色滤光膜硅覆液晶微显示器中像素尺寸及滤光膜厚度对彩色侧向场效应的影响

应用彩色滤光膜硅覆液晶(Color Filter Liquid Crystal on Silicon，CF-LCoS)微显示器件的三维光学模型; 研究了像素尺寸和彩色滤光膜厚度对彩色侧向场的影响。这两个参数可以影响微显示器件的色纯度、光反射率以及对比度等性能参数。对于特定的应用，需要考虑器件所有的性能参数要求，才能对像素尺寸和彩色滤光膜厚度进行优化。The relation of color fringing field with the pixel size and thickness of color filters was researched in this paper, and the study was based on the three-dimensional (3D) optical modeling we established before. These two parameters may affect the color, reflectivity, contrast, and other properties of the CF-LCoS microdisplays. The optimization of pixel size and thickness of color filters should be compromised among these display properties for specific applications

基于“协同育人”平台的工业设计实践教学条件共建路径

根据“新工科”应用型人才培养目标要求，为落实“中国制造 2025”国家战略，开展基于“协同育人”平台开展高等学校的实践教学的立体式校企合作与校际合作，兼顾校本位教学实际改善与学生毕业后职业适应度提高，注重实践环节社会主义核心价值观认同度提高教育，利用该教学改革应用的成果，是培养提供工程与管理知识兼备的复合型应用技术优秀人才的有效路径。</jats:p

新型Bola两亲分子作为基因治疗药物输递载体的研究

为检测新型Bola两亲分子（Orn-C16-G）用作基因载体的性能,制备了Bola与siRNA的复合物（Bolaplexes）,用原子力显微镜观察其在不同复合比下的形貌;并转染Hela细胞,用CCK-8检测Bolaplexes的细胞毒性,用荧光显微镜、流式细胞仪表征Bolaplexes的细胞内吞效果。实验结果显示,复合比1∶1条件下,Bola分子可以有效地压缩siRNA分子形成较小尺寸的复合物（100～200 nm）。Bolaplexes可高效地进入Hela细胞,并且毒性低于商售转染试剂,有较好的应用前景。本研究为下一步Bola两亲分子用于基因治疗的临床研究奠定了基础