Function analysis of VP466-Rab-tropomyosin-actin complex in shrimp immunity and white spot syndrome virus (WSSV) infection

对虾白斑综合症病毒（WhiteSpotSyndromicVirus,WSSV），是一种严重危害对虾养殖业的主要病原。本论文从病原和宿主两方面入手，着重探讨了WSSV病毒的主要膜蛋白VP466与对虾体内蛋白Rab、原肌球蛋白（tropomyosin）以及肌动蛋白（actin）之间形成的复合体（complex）在对虾的免疫和病毒的感染过程中的所起的重要作用，不仅为在宿主体内寻找病毒膜蛋白受体提供了理论基础而且为建立有效的防治方法提供了科学依据。 本论文首先证明了VP466-Rab-tropomyosin-actin是一个复合体。根据GST-pulldown、质谱的结果，发现Rab蛋白与对虾白斑综...White spot syndrome virus (WSSV) is one of the major pathogens in the worldwide shrimp aquaculture. The paper was started at two aspects including virus and host, and the important role of VP466-Rab-tropomyosin-actin complex in shrimp immunity and in WSSV infection was emphatically studied. Our results will not only provide strong theoretical basis for finding WSSV membrane repcepor in shrimp but ...学位：理学硕士院系专业：生命科学学院生物学系_水生生物学学号：2012005130200

The Composition and Evolution of Southern Jiangxi’s Local Military System in Ming Dynasty——Also On Local Militarization in Late Ming Dynasty

在传统中国，军事组织作为重要的政治与社会组织，探讨其中各种军事力量的构成、表现形式及其盛衰演变，有助于了解传统社会政治结构的特点及其历史变迁。本文着眼于“区域史”的角度，以明代赣南为研究个案，试图通过考察明代赣南地方军事体系的构成及其历史演变，探讨和揭示明中叶以后地方军事化的早期历史过程。 明代赣南的地方军事体系，包括了三种军事力量，既有明初建立的赣州卫和会昌、信丰、南安三个千户所以及后来出现的各地营兵，是属于国家/中央常备军事力量；也有明宣德至正统年间以来各府县大力组织的民兵民壮，是属于地方政府领导的地方防卫力量；还有就是明中叶前后广泛兴起的民间自发组织的乡兵义勇，是属于乡村自卫的乡族武装...In traditional China, the military organization was an important political and social organization. Investigating the composition, the form and the evolution of the various military forces is helpful to understand the characteristics and the history of political and social structure in traditional China. From the perspective of regional history and on the case study of southern Jiangxi, This arti...学位：历史学硕士院系专业：人文学院历史学系_专门史学号：1032007115157

基于碳烟颗粒催化氧化的三维有序大孔催化剂制备及性能

三维有序大孔钴锰尖晶石催化剂（3DOMCoMn2O4）以及三维有序大孔镧、铈掺杂的钴锰尖晶石催化剂(3DOMRxCo1-xMn2O4（R=Ce, La）)由胶晶模板法成功合成。通过对所得的催化剂在NOx协助下的碳烟催化氧化活性评价,优化了Ce/Co和La/Co的配比。此外,还对目标催化剂进行了XRD、N2吸脱附、Raman、H2-TPR、SEM、XPS等表征。结果表明:3DOM结构增强了催化剂与碳烟颗粒之间的接触,对碳烟氧化等\"固-固-气\"非均相催化反应具有明显的提升作用。此外,铈和镧的掺杂增大了活性氧物种的浓度,从而增强了钴锰尖晶石催化剂的催化氧化能力。在松散接触工况下,3DOM Ce0.9Co0.1Mn2O4的Tig（起燃温度）和Tm（CO2出口浓度最大时的温度）分别为285℃和377℃,3DOM La0.3Co0.7Mn2O4的Tig和Tm分别为287℃和376℃。国家自然科学基金资助项目(21276214);;福建省科技厅重点项目(2016H6024);;福建省自然科学基金资助项目(2015J05033

Effects of electropuncture of acupoints on the foot Yangming stomach meridian on metabolic profiling of gastric mucosa in gastric ulcer rats

目的:采用核磁共振氢谱技术(1H; NMR)研究电针胃经穴对应激性胃溃疡大鼠胃黏膜组织代谢物谱的表达。方法:40只SD大鼠随机分为正常组、模型组、胃经组和胆经组。利用束缚-冷应激法; 制备应激性胃溃疡模型大鼠,光镜下观察大鼠胃黏膜组织病理形态学的变化,采用1H NMR技术获取胃黏膜组织1H; NMR谱,并利用模式识别方法分析各组间的代谢物谱的差异。结果:与正常组比较,模型组大鼠胃黏膜损伤和胃黏膜组织代谢轮廓差异明显,胃黏膜组织乙酸、肌; 醇、磷酸胆碱、谷氨酸、甘氨酸、乙醇胺、赖氨酸、3-羟基丁酸、缬氨酸、异亮氨酸、牛磺酸、亮氨酸和甘露醇含量上升(P<0.05),乳酸、甲硫氨酸和天; 冬氨酸含量下降(P<0.05);与模型组比较,胃经组和胆经组大鼠胃黏膜得到明显修复,胃经组大鼠胃黏膜组织代谢轮廓更接近正常大鼠,胃黏膜组织甲硫氨; 酸、天冬氨酸含量上升(P<0.05),乙酸、肌醇、磷酸胆碱、谷氨酸、甘氨酸、赖氨酸、异亮氨酸、牛磺酸、乳酸、亮氨酸和甘露醇含量下降(P<0.05; );胆经组大鼠胃黏膜组织3-羟基丁酸、甲硫氨酸含量上升(P<0.05),肌醇、磷酸胆碱、谷氨酸、乙醇胺、异亮氨酸、乳酸、亮氨酸和甘露醇含量下降(; P<0.05)。结论:电针胃经穴可调节胃溃疡大鼠胃黏膜组织特异代谢物的表达,促进胃黏膜损伤的修复。Objective: To research the effects of electropuncture of acupoints on; the foot Yangming stomach meridian on metabolic profiling of gastric; mucosa in rats of gastric ulcer induced by stressusing the 1H nuclear; magnetic resonance (1H NMR). Methods: Forty SD rats were randomly; divided into the control group, the model group, the stomach meridian; group and the gallbladder meridian group. The rat model of gastric ulcer; was induced by the restrained plus cold stress. The pathological changes; in the rat gastric mucosa tissue were observed by light microscope. The; 1H NMR spectroscopy was determined with the 1H NMR technique. And,; meanwhile the differences in the metabolic profiling were identified; with the pattern recognition method. Results: Compared to normal group,; the gastric mucosa damage and gastric mucosa metabolic profiling are; obvious, the concentration of acetic acid, choline, inositol phosphate,; glutamic acid, glycine, ethanolamine, lysine, 3-hydroxy butyric acid,; valine, isoleucine, taurine, leucine and mannitol increase and the; concentration of lactic acid, methionine and aspartic acid decrease in; model group rats (P<0.05). Compared to model group, the gastric mucosa; damage have been recovered in stomach meridian group and gallbladder; meridian group, the metabolic profiling of gastric mocusa tissue in; stomach meridian group are similar to the normal group. The; concentration of methionine, aspartic acid increase and acetic acid,; choline, inositol phosphate, glutamic acid, glycine, lysine, lactic; acid, leucine, isoleucine, taurine, and mannitol decrease in stomach; meridan group (P<0.05). The concentration of 3-hydroxy butyric acid,; methionine increase and choline, inositol phosphate, lactic acid,; leucine, isoleucine, glutamic acid, ethanol amine and mannitol decrease; in gallbladder meridian group (P<0.05). Conclusion: The electropuncture; of acupoints on the foot Yangming stomach meridian could regulate the; metabolic profiling of gastric mucosa, resulting in improving the; gastric mucosa repair of gastric ulcer rats.国家自然科学基金项目; 国家重点基础研究发展计划(973计划)项目; 广东省自然科学基金项目; 深圳市科技计划项

Improvement of electrochemical reduction device for detection of bromate in wheat flour

依据电解、电渗析和电迁移原理改进电化学还原装置,在不使用化学试剂的条件下,将待测样品组分中的br O-3还原为br-后进行检测,可避免高浓度Cl-的干扰。溴酸盐实样的检测表明,溴酸盐检出限为0.32 Mg/kg,日内精密度为2.73%,日间精密度为3.81%,回收率可达92%~96%。An electrochemical reduction device developed according to electrolysis,electrodialysis and electromigration theories can reduce bromate to bromide without any use of reagent.The high concentration chloride in samples would interfered the detection of bromate by Ion Chromatography,but this defect could be overcome if bromate is reduced to bromide before detection.When useing the electrochemical reduction device to detect bromate in real samples,the detection limit of bromate was 0.32 mg / kg,the within day precision was 2.73%,the between days precision was 3.81%,and percent recovery was 92% ~ 96%.国家自然科学基金(20527005); 公益性行业科研专项(2012104012-3

Determination of trace amounts of gold(III) by cathodic stripping voltammetry using a bacteria-modified carbon paste electrode

A bacteria-modified carbon paste electrode has been prepared and used for the very sensitive and selective determination of trace amounts of gold(III). The modified electrode was able to detect a solution of 1.0 ppb Au(III) by applying cathodic stripping voltammetry. Advantages of the bacteria-modified electrode include high sensitivity, good stability, low cost and simple preparation. It could be a new class of modified electrode with practical value

Five-electrode direct current suppressor-detector combiner for ion chromatography: an integration of eluent suppression and resistance detection

An integration of eluent suppressor and resistance detector is proposed based upon the application of a common anode (or cathode), a common ion-exchange membrane, and two independent constant current sources.National Natural Science Foundation of China[20527005

Determination of Glucose in Serum by Enzyme-Ion Chromatography

利用葡萄糖氧化酶对β-D-葡萄糖催化氧化的高度专一性,催化性能高的优点,催化血清中β-D-葡萄糖被溶液中的溶解氧氧化为D-葡萄糖酸,同时结合离子色谱法快速、灵敏、准确的特点,检测生成的D-葡萄糖酸,并用标准工作曲线法来定量血清中的葡萄糖的含量,建立起酶-离子色谱法检测血清中葡萄糖的新方法.该法线性范围为1.00~90.00mg/L.测得值与采用贝克曼CX3测得值相比,误差为-2%~2%.β-D-Glucose in serum was oxidized by the oxygen dissolved in solution with the catalysis of glucose oxidase based on its high selectivity to glucose. We used the ion chromatography to determine the production, D-glucose acid and then the concentration of glucose in serum could be quantitatively determined through a standard working curve with a linear range of 1.00～90.00 mg/L.A new method has been developed to determine the glucose in serem.福建省重大科技项目资助(2003Y010

Constructing a Modern Chemical Experimental Teaching Demonstration Centre

介绍了厦门大学化学化工学院在深化实验教学改革,建设现代化的国家级实验教学示范中心方面的一些做法。全文分6个部分对实验教学体系与管理,教学内容改革与教师队伍建设等几方面进行了讨论。This paper introduced the reform of experimental teaching the school of chemistry and chemical engineering,Xiamen University did to establish a national modern chemical experimental teaching demonstration centre.A new teaching system including teaching methods,construction of teacher team and the management of experimental teaching were discussed.依托优势学科,构筑高水平大学创新性人才培养跨学科立体实验教学平台(教育部高等教育理工教育教学改革与实践项目