嗜人类T淋巴细胞病毒Ⅰ型核心蛋白P24基因的克隆与表达

目的 从感染HTLV-1的中国患者外周血单核细胞（PBMCs）中扩增编码HTLV-1核心蛋白P24的cDNA,并在大肠杆菌中进行表达。方法 提取感染者PBMCs基因组DNA,应用巢式PCR方法扩增出HTLV-1核心蛋白P24的cDNA并测序,与pGEX-20T载体构建重组质粒,在大肠杆菌BL21中表达,采用ELISA和Westernblot分析重组蛋白活性。结果HTLV-1核心蛋白P24基因序列高度保守,构建重组载体后,在大肠杆菌中表达的重组蛋白,经检测具有较强的抗原活性。结论 成功地表达了HTLV-1型病毒的核心蛋白P24基因,为国产诊断试剂和疫苗的研发打下了基础

云南鲁甸6.5级地震灾害特点浅析/Hazard Analysis on the Characteristics of the M6.5 Ludian Earthquake[J]

通过对2014年8月3日云南鲁甸6.5级地震震害开展实地调查,对灾区破坏情况进行总体介绍,并就各烈度区特征和建筑物震害、地震地质灾害、工程结构震害进行分析,初步得出本次地震的一些震害特点.一是灾区人口密度大,人员死亡较集中.人员死亡主要集中在Ⅷ和Ⅸ度区.二是地震振动强,灾区破坏严重.本次地震震源深度12km,极震区烈度高达Ⅸ度,震源破裂在11s内集中释放.三是抗震能力弱,房屋破坏严重.灾区属国家级贫困区,农村民居抗震能力弱,且多数民房坐落在河谷陡坡上,边坡效应加重房屋震害,重灾区砖木和土木房屋成片损毁、倒塌.四是灾区条件恶劣,救灾难度大.震区活动断裂密集发育、地质破碎疏松、地形崎岖不平,又恰值雨季,诱发极其严重次生地质灾害,导致人员伤亡,造成灾区大面积交通、通信、电力中断,救援物资与救援力量无法及时发挥作用

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements